Effective part of spreading hedyotis herb and preparation method thereof

A technology of Hedyotis diffusa and effective parts, applied in the field of medicine, can solve the problems of complexity, many active ingredients, no preparation method and use of Hedyotis diffusa effective parts, etc., and achieves improved stability and obvious anti-tumor effect. Effect

Inactive Publication Date: 2011-10-19
CAPITAL NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, many scholars have studied the anti-tumor effect of Hedyotis diffusa, and its anti-tumor active ingredients are many and relatively complex.
But so far no patent related to the effective parts of Hedyotis diffusa and its preparation method and use

Method used

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  • Effective part of spreading hedyotis herb and preparation method thereof
  • Effective part of spreading hedyotis herb and preparation method thereof
  • Effective part of spreading hedyotis herb and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0175] Example 1: Preparation of effective parts of Hedyotis diffusa of the present invention

[0176] Extraction: take the medicinal material Hedyotis diffusa and add 12 times by weight of 60% ethanol, extract twice for 2 hours each time, combine the extracts, and concentrate;

[0177] Purification: Extract the aqueous solution of the concentrate with petroleum ether for 3 times, remove the petroleum ether layer, put it on a D-101 macroporous resin column, the loading concentration is 3.733mg / mL, and perform dynamic adsorption according to the adsorption flow rate of 4mL / min. The height ratio is 1:10, elute with water, 20%, 30%, and 40% ethanol solution at a flow rate of 3 mL / min, collect 40% of the eluted part and evaporate to dryness, the temperature is not higher than 60 ° C, after vacuum drying The effective part of Hedyotis diffusa was obtained, and the content of 6-0-p-coumaroyl gallinetoside in the effective part was 70%.

Embodiment 2

[0178] Example 2: Preparation of effective parts of Hedyotis diffusa of the present invention

[0179] Extraction: take the medicinal material Hedyotis diffusa and add 9 times by weight of 60% ethanol, extract 3 times, each time for 2.5 hours, combine the extracts, and concentrate;

[0180]Purification: Extract the aqueous solution of the concentrate with petroleum ether for 5 times, remove the petroleum ether layer, put it on a D-101 macroporous resin column, the loading concentration is 4.825mg / mL, and perform dynamic adsorption according to the adsorption flow rate of 5mL / min. The height ratio is 1:12, elute with water, 25%, 40%, 55% ethanol solution at a flow rate of 5mL / min, collect 50% of the eluted part and evaporate to dryness, the temperature is not higher than 60 ° C, after vacuum drying The effective part of Hedyotis diffusa is obtained, and the content of 6-0-p-coumaroyl gallinetoside in the effective part is 80%.

Embodiment 3

[0181] Example 3: Preparation of effective parts of Hedyotis diffusa of the present invention

[0182] Extraction: take the medicinal material Hedyotis diffusa and add 15 times by weight of 60% ethanol, extract once, 2 hours each time, combine the extracts, and concentrate;

[0183] Purification: extract the aqueous solution of the concentrate twice with petroleum ether, remove the petroleum ether layer, put it on a D-101 macroporous resin column, the loading concentration is 2.8mg / mL, and perform dynamic adsorption according to the adsorption flow rate of 3mL / min. The height ratio is 1:8, elute with water, 15%, 25%, and 30% ethanol solution at a flow rate of 2.8mL / min, collect 30% of the eluted part and evaporate to dryness, the temperature is not higher than 60 ° C, and vacuum dry Afterwards, the effective part of Hedyotis diffusa is obtained, and the content of 6-0-p-coumaroyl gallinetoside in the effective part is 65%.

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Abstract

The invention discloses an effective part of spreading hedyotis herb and a preparation method thereof. The preparation method comprises the following steps of: purifying the effective part of the spreading hedyotis herb, determining the content of total iridoid glycoside and 6-O-p-coumaroyl scandoside methyl ester in the effective part of the spreading hedyotis herb, and further defining effective anti-tumor ingredients in the effective part; the preparation method is beneficial to increase of the stability of products; meanwhile, mice anti-tumor experiments and the contrast between the effective part and a clinically commonly-used anti-tumor medicine, namely, cyclophosphamide, show that the effective part of the spreading hedyotis herb has an obvious anti-tumor effect.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to an effective part of Hedyotis diffusa, a preparation method, a detection method thereof, and an application thereof in the preparation of antitumor drugs. Background technique [0002] Hedyotis diffusa is a traditional Chinese medicine commonly used by the people. It tastes bitter, sweet, and cold in nature, and it returns to the heart, lung, liver, and large intestine meridians. The main functions are heat-clearing and detoxifying, dampness-reducing, anti-cancer, mainly treating lung-heat cough, sore throat, intestinal abscess and sore, poisonous snake bite, hot stranguria, edema, dysentery, enteritis, damp-heat jaundice, cancer, etc. In recent years, many scholars have studied the anti-tumor effect of Hedyotis diffusa, and its anti-tumor active ingredients are many and complex. But so far, no patent related to the effective part of Hedyotis diffusa and its preparation method ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/748A61P35/00G01N21/31G01N30/02
Inventor 王英锋郑宜
Owner CAPITAL NORMAL UNIVERSITY
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