Fingered citron beer and brewing method thereof
A bergamot and beer technology, applied in the field of bergamot beer and its brewing, can solve problems such as difficulty in development and utilization, and achieve the effects of scavenging oxygen free radicals, promoting gastrointestinal motility, and promoting appetite
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[0019] Example 1
[0020] (1) The growth of different yeasts in the bergamot juice medium
[0021] Because bergamot contains bitter substances that inhibit the growth of microorganisms, it is not known what effect it has on the growth and fermentation of yeast while it is used to impart a bitter taste to beer. To successfully ferment the bergamot beer, it is necessary to have the ability to A yeast strain grown and fermented in bergamot juice or bergamot-malt mixture.
[0022] Fifteen strains of yeast were activated by a slope and spotted on the agar plate of pure bergamot juice. They were cultured statically at 28°C. The colony size of the strains was observed, and the results were as follows figure 1 Shown; by comparison figure 1 The size of the colony before and after the culture in the plate showed that pure bergamot juice had different effects on the growth of different yeasts, among which bacteria 1, 3, 4, 5, 7, 12, 15 grew relatively well.
[0023] The whole wort with the sam...
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[0042] Example 2 Production and fermentation of bergamot beer
[0043] (1) Expansion of Saccharomyces cerevisiae (CGMCC No.6822)
[0044] 1 ring of slant bacteria sample → 1 9mL wort test tube (28°C, 48 hours) → 24 9mL wort test tubes each with 1 mL bacterial solution (25°C, 24 hours) → 2 wort test tubes with culture solution connected to 180mL wheat In the juice (23°C, 48 hours)→correspondingly insert a 1.8-liter wort triangle flask (21°C, 36 hours)→the culture broth is settled in the refrigerator for 12 hours, the clear liquid is decanted, and the yeast puree is reserved.
[0045] (2) Preparation of fermented wort
[0046] After the barley malt is crushed, add 50℃ water according to the material-to-water ratio of 1:3.8, stir and keep for 50min, increase to 63℃ at a heating rate of 1℃ / min, keep for 1 hour, continue heating to 72℃, 0.02mol / L dilute iodine Iodine test until the iodine solution does not change color, keep it for 20 minutes, and finally heat up to 78°C to filter the wor...
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