181 results about "Limonin" patented technology

The invention provides a method for extracting active ingredients from tangerine seeds. The method comprises the following steps of: crushing the tangerine seeds, performing supercritical extraction of tangerine oil, performing immersion and homogenizing treatment on oil-removed tangerine seed residue, performing centrifugal separation on the homogenate, performing ultra-filtration treatment on supernate A, and performing affinity chromatography on filtrate and the like to finally obtain limonin with high purity and other active ingredients such as acidic limonin aglycon, nomilin, neutral limonin aglycon (containing no limonin or nomilin) and the like extracted from the waste tangerine seeds so as to realize comprehensive utilization of a tangerine seed resource. The method has high yield, the consumption of an organic solvent is greatly reduced in the extraction process, the flammable and combustible problems caused by extraction with the organic solvent are avoided, the production cost is reduced, and the environmental pollution is reduced.

The invention discloses a method for continuously extracting orange oil and limonin from citrus reticulate by using supercritical carbon dioxide, and belongs to the fields of grease extraction methods and natural product separation. A process flow comprises the following steps of: drying and crushing citrus reticulate tangerine seeds; performing supercritical extraction; separating mandarin oil from residues; performing supercritical extraction; separating; and crystallizing and purifying to obtain the limonin. The method has the advantages of high product yield and no solvent residue. By continuously extracting the orange oil and the limonin from the tangerine seeds, full utilization of the waste citrus reticulate tangerine seeds is realized, the high-quality orange oil and high-purity limonin are obtained, the production cost is reduced, and the added value and the economic benefit of a product are improved.

The invention relates to a high performance liquid chromatography-mass spectrometry (HPLC-MS) quantitative method for measuring 10 components of a fructus evodiae formula granule. The method is characterized in that a HPLC-MS technology is used; acetonitrile is taken as the A phase; acetic acid (0.12%)-ammonium acetate (1 mmol/L) is taken as the B phase; gradient elution with four scale transformations is performed; the flowing speed is 0.8 mL/min; the column temperature is 40 DEG C; in a multiple reaction monitoring (MRM) mode, positive ions and negative ions are monitored at the same time, the ion source is an ESI source, the atomized gas (GS1) is N2, the auxiliary gas (GS2) is N2; nitrogen gas is used during the whole process; the collision gas pressure is medium; the resolutions of Q1 and Q3 are both UNIT; the method can detect 10 components in the fructus evodiae formula granule, namely chlorogenic acid, caffeic acid, rutin, hyperin, hesperidin, dehydroevodiamine, limonin, evodiamine, rutaecarpin, and evocarpine; 10 components can be all detected in 22 minutes, and the method is rapid and accurate and is not disturbed.

The invention relates to a method for extracting limonin substances from citrus limon seeds as a raw material. The method comprises the following steps: crashing the citrus limon seeds; adding water and an enzyme preparation for enzymolysis; standing; discarding enzymolysis liquid; adding alcohol to sediment for leaching; adopting the absorption chromatographic separation of leaching liquid to obtain the limonin substances; and drying the limonin substances. The method uses lemon product waste, i.e. lemon rods as the raw material so as to not only fully utilize the waste generated in the production process, lessen the waste of the raw material and reduce the environmental pollution, but also obtain the limonin substances with high content up to 95 percent and extraction rate up to 90 percent which is higher than that of the prior extraction rate by 30 percent, and adopts ethanol digestion, macroporous resin column chromatographic separation and silica gel chromatographic column purification to decrease the production cost.

The invention relates to compositions and methods for preventing, treating, or managing anxiety, pain, chronic pain, depression, and disorders such as premenstrual syndrome comprising the administration of a prophylactically and therapeutically effective amount of Rutaceae plant or extracts thereof to a mammal in need of such therapy. In a preferred embodiment, the mammal is human and the extracts are substantially free of the compounds obacunone or limonin. The invention also relates to compositions and methods for preventing, treating, or managing separation anxiety in domestic animals comprising the administration of a prophylactically and therapeutically effective amount of Rutaceae plant or extracts thereof to an animal in need of such therapy.

The invention provides a method for preparing a composition comprising high-purity limonin and a medicinal composition which is prepared by the method, has high safety and comprises the high-purity limonin. In the preparation method, the limonin of which the purity is over 98 percent can be obtained under the condition of recrystallization and purification for two times; and the impurities in the limonin do not damage the safety. Moreover, the invention also provides application of the medicinal composition in preparing a medicament for preventing and/or treating the colon cancer or the rectal cancer.

The invention discloses a method for extracting limonin analogues from tangerine seeds. The method comprises the following steps: firstly, obtaining raw materials, wherein the raw materials are subjected to ultrasonic extraction with 90% ethanol for 1 hour to obtain an extract, the extract is directly detected, the content of limonin is required to be equal to or greater than 0.6% and the content of nomilin is equal to or greater than 0.3%; secondly, breaking hulls of the raw materials and pulverizing until the hulls of the raw materials are completely broken; thirdly, extracting the raw materials with 90% ethanol for three times and 1.5 hours each time and ultimately controlling that the active ingredients in the residue account for no more than 3% of the raw material total target, wherein the ratio of material to solvent is 1 to 4 to 4 to 3; fourthly, concentrating the extract until the degree of alcohol is 20%, standing for 12 hours and removing floating oil on the surface of the liquid; fifthly, continuously recovering the solvent from a concentrated solution, controlling the extraction specific gravity to be 1.03+/-0.1, standing and crystallizing for 12 hours; sixthly, extracting an upper layer supernatant, filtering the lower layer precipitate with filter cloth and centrifuging; and seventhly, after centrifuging, carrying out coarse crystallization and vacuum drying at a temperature of less than 80 DEG C.

The invention provides a process for preparing limonin monomer by an industrial chromatography method. The process comprises the following steps: extracting oil from citrus seeds after being washed and dried by alcohol organic solvent, filtering and separating to obtain filtrate; decompressing and concentrating the filtrate, recycling the alcohol organic solvent, extracting by hydrochloric ether and concentrating to form extract; refluxing and extracting the extract by ether solvent, degreasing the extract, obtaining filtration residues by filtering and washing the filtration residues until no fatty acid exists; adding alcohol solvent into the filtration residues, carrying out recrystallization to obtain coarse crystal containing a plurality of limonin compounds, crystallizing and drying to obtain limonin compound crystal after carrying out the chromatography by an industrial chromatographic column. The process fully utilizes waste citrus seeds and adopts the industrial chromatography method to extract high-purity limonin, improves the comprehensive utilization ratio, has low cost, easy operation and control, repeated solvent utilization and reduced pollution and is suitable for industrialized large-scale production.

The invention discloses a method for debitterizing lemon juice, belonging to the technical field of beverage preparation. The method comprises the steps as follows: firstly, selecting an undamaged lemon, cleaning, peeling, juicing, filtering and centrifuging to obtain the lemon juice; secondly, putting the lemon juice into a water bath kettle being 85-95 DEG C, deactivating enzyme for 8-15min, and cooling; thirdly, measuring the bitter substance content and the Vc content of the lemon juice; fourthly, adding 0.3-0.7g of beta-cyclodextrin into every 100mL of the lemon juice subjected to enzyme deactivation, uniformly mixing, putting a mixture into the water bath kettle, and processing for 60-120min at the temperature of 30-50 DEG C; and lastly, measuring the bitter substance content and the Vc content of the debitterized lemon juice, and calculating the debitterizing rate and the loss rate of the lemon juice. According to the method provided by the invention, the removal rate of bitter substances-limonin and naringin in the lemon juice is high, the loss rate of Vc is low, and the lemon juice with good flavor can be obtained; and meanwhile, the cost is low, the required equipment is simple and the processing operation is convenient.

The invention relates to a process for hierarchically extracting limonin, flavonoid and dietary fiber from orange peel residue, and the orange peel residue is used as a raw material. According to different properties and characteristics of limonin, flavonoid and dietary fiber to be extracted, different methods and technical processes are adopted after primary production to avoid mutual interference among the contents and hierarchically and sequentially extract limonin, flavonoid, soluble dietary fiber and insoluble dietary fiber. Therefore, the invention achieves purpose of decreasing the cost, improving the efficiency, simplifying the process and respectively extracting a plurality of contents.

The invention discloses a grapefruit pericarp and pomace extractive preparation method and a preparation containing the extractive and belongs to the preparation method of plant active ingredients. The preparation method comprises the following steps: sequentially cutting up, drying and smashing fresh grapefruit pericarp and pomace to obtain powder; using a 95% ethanol solution to perform heating reflux on the powder for extraction so as to obtain an oily extractive; adding petroleum ether into the oily extractive for ungrease treatment so as to obtain a nonfat extractive; utilizing D101 macroporous resin to perform column chromatography on the nonfat extractive, using ethyl alcohol to perform elution, collecting the eluant, and performing rotary evaporation on the eluant to obtain the grapefruit pericarp and pomace extractive. According to the invention, the grapefruit pericarp and pomace waste is used as the raw material to establish the effective ingredient extractive technology, extract limonin and flavonoid substances, research and develop capsula capable of reducing blood fat and meet the demand of the current blood fat reduction health care product market, so that the grapefruit pericarp and pomace extractive has broad market prospects.

The present invention discloses a one-step debitterizing and reducing acidity method of high pulp content juices, and belongs to the technical field of juice preparation. The method includes the following steps: juicing fresh fruits and obtaining a raw juice, centrifuging the raw juice to obtain a crude separation of juice and pulp, pumping the crude separation juice into a buffer tank, pumping the crude separation pulp into a recovery tank for the subsequent backfilling; carrying out high-speed centrifugal separation of the crude separation juice and obtaining a fine separation of juice and pulp, pumping the fine separation juice into the buffer tank, and carrying out sterilizing and inactivating enzyme at a temperature of 85 to 90 DEG C for 10s to 30s, pumping the fine separation pulp into the recovery tank for the subsequent backfilling; after sterilizing and inactivating enzyme, pumping the fine separation juice from the buffer tank to a mixed resin tank, carrying out debitterizing and reducing acidity; backfilling the pulp, carrying out vacuum concentration and obtaining the high pulp content juices. The method can realize debitterizing and reducing acidity in one resin tank, maximize retain juice flavonoids, VC and other nutrients of the juice on the premise of removing limonin and fruit acid, and ensure the juice quality.

The invention discloses a acetobacter pasteurium and its application method in producing citrus vinegars and resulting citrus vinegar products. The invention provides acetobacter pasteurium named LSYY001.2, preserved in CGMCC on 14th June, 2010, preservation number: CGMCC No.4954. Method of the invention for producing citrus vinegar comprises: selecting materials, peeling citruses, removing segment membranes, crushing, filtering, adding yeasts for alcoholic fermentation, adding LSYY001.2 for acetic acid fermentation, then filtering. The acetobacter pasteurium provided by the invention not only ferments alcohol for producing acetic acid, but simultaneously produces iso-limonin hydrolase, thus realizing removing bitter of the citrus vinegar.

The invention discloses a raw material formula of the lemon lozenge and a preparation process thereof. The raw material formula comprises the following raw materials: lemon powder or lemon concentrated solution, excellent powdered sugar or xyllitol, glycyrrhizin, starch, one or more of lemon yellow or sunset yellow and lemon oil. The lemon lozenge solves the problem that the lemon is peracid and diet and cannot be directly eaten, and solves the technical problems for directly performing the fruit powder. The lemon lozenge has the advantages of effective biological activity and easy absorption, and maintains a great amount of nutrients and Vc of the lemon. The product is also a leisure product with low calorie and functions, not only maintains the original unique flavor of the lemon, but also maintains the biological activity and the unique pharmaceutical value of the lemon. The product is rich in vitamins, organic acid, limonoid, flavonoid and hemiterpene substances, can moisten the throat, and can diminish the inflammation. Organic acid can resist the fatigue, activate the blood vessel, and can promote the digestion of the human body, and also has the auxiliary functions for clearing the intestines and stomach, reducing the cholesterol, improving the appetite, and the like.

The invention discloses an extraction method of limonin. The extraction method comprises the following steps: pretreatment: adding lemon seeds and lemon peel residues into water, carrying out soaking,and adding a pretreatment enzyme to decompose impurities; extraction: adding an extracting enzyme into a pretreatment product for enzymolysis, adding acid for acidolysis, performing solid-liquid separation, removing solid residues, and reserving liquid to obtain a primary product; removal of pectin: carrying out vacuum concentration on the primary product, adding alcohol for alcohol precipitation, separating a floccule precipitate, and reserving liquid; and purification: carrying out extracting at first, and then conducting separating by using macroporous resin. The extraction method disclosed by the invention is reasonable in design, all the steps are associated with one another, impurities are removed one by one, and extraction and macroporous resin separation are combined for use; andextraction is carried out at first and then macroporous resin separation is carried out, and the advantages of two separation means are combined, so separation efficiency is high, and product purity is high.