High performance liquid chromatography-mass spectrometry (HPLC-MS) quantitative method for measuring 10 components of fructus evodiae formula granule

A technology of ultra-high performance liquid phase and formula granules, which is applied in the field of quantitative determination of 10 components in Evodia rutaecarpa formula granules by ultra-high performance liquid chromatography mass spectrometry, achieving high accuracy, high selectivity, and the effect of expanding the detection range

Active Publication Date: 2017-09-05
HEBEI UNIV OF CHINESE MEDICINE +1
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  • High performance liquid chromatography-mass spectrometry (HPLC-MS) quantitative method for measuring 10 components of fructus evodiae formula granule
  • High performance liquid chromatography-mass spectrometry (HPLC-MS) quantitative method for measuring 10 components of fructus evodiae formula granule
  • High performance liquid chromatography-mass spectrometry (HPLC-MS) quantitative method for measuring 10 components of fructus evodiae formula granule

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[0059] (1) Detection conditions Ultra-high performance liquid chromatography: chromatographic column: Diamonsil C 18 Column (150×4.6mm, 5μm); Acetonitrile is used as phase A, 0.12% acetic acid / 1mmol / L ammonium acetate is used as phase B, and gradient elution is adopted, that is, 0~6min, 15%~28%A; 6~10min , 28%~68%A; 10~11min, 68%~90%A; 11~22min, 90%A; flow rate 0.8mL / min; column temperature 40℃; The positive and negative ions are monitored at the same time in the scanning mode of monitoring (MRM), the ion source is ESI source, the ion spray voltage is set to 5500 and -4500V, the source temperature (TEM) is 650°C, the atomizing gas (GS1, N2) is 60psi, and the auxiliary The gas (GS2, N2) is 65psi, the interface is continuously heated, nitrogen is used throughout the process, the curtain gas (N2) is set to 25psi, the collision gas pressure is medium, the resolution of Q1 and Q3 is UNIT; the monitoring ion pair of each compound to be tested is the molecular ion peak and product i...

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Abstract

The invention relates to a high performance liquid chromatography-mass spectrometry (HPLC-MS) quantitative method for measuring 10 components of a fructus evodiae formula granule. The method is characterized in that a HPLC-MS technology is used; acetonitrile is taken as the A phase; acetic acid (0.12%)-ammonium acetate (1 mmol/L) is taken as the B phase; gradient elution with four scale transformations is performed; the flowing speed is 0.8 mL/min; the column temperature is 40 DEG C; in a multiple reaction monitoring (MRM) mode, positive ions and negative ions are monitored at the same time, the ion source is an ESI source, the atomized gas (GS1) is N2, the auxiliary gas (GS2) is N2; nitrogen gas is used during the whole process; the collision gas pressure is medium; the resolutions of Q1 and Q3 are both UNIT; the method can detect 10 components in the fructus evodiae formula granule, namely chlorogenic acid, caffeic acid, rutin, hyperin, hesperidin, dehydroevodiamine, limonin, evodiamine, rutaecarpin, and evocarpine; 10 components can be all detected in 22 minutes, and the method is rapid and accurate and is not disturbed.

Description

technical field [0001] A quantitative method for the determination of 10 components in Evodia rutaecarpa formula granules by ultra-high performance liquid chromatography-mass spectrometry, which belongs to the field of quality control, that is, an ultra-high performance liquid chromatography and a mass spectrometer are connected in series, the liquid chromatography is used to separate the mixture, and the mass spectrometer It can detect and quantitatively determine multiple components, and has the characteristics of high sensitivity, high selectivity, and high accuracy. The determination of 10 components can be completed within 22 minutes. It is no longer troubled by different optimal detection wavelengths, different levels of content, and great differences in polarity for different components. The detection limit can be reduced from the μg level of HPLC to the ng level, which greatly expands the detection range. Background technique [0002] Evodia rutaecarpa (Juss.) Benth....

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 牛丽颖王晓亚王鑫国
Owner HEBEI UNIV OF CHINESE MEDICINE
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