This invention discloses an immune
cell with enhanced anti-tumor effect, its preparation method, and its application. Experiments using this invention show that... SERTAD1 CD8
gene overexpression + T cells can enhance their function in anti-tumor
immunotherapy. SERTAD1
Gene overexpression can downregulate CD8 + The expression of
immune checkpoint molecules on T cells upregulates the expression of mRNAs of tumor-killing cytokines. In the MC38-OVA
subcutaneous tumor model, Naïve CD8 cells were extracted from OT-I mice. + T cells were infected with a
virus to overexpress SERTAD1, followed by adoptive transfer to mice. The results showed that SERTAD1 overexpression enhanced CD8 expression. + T cells kill MC38-OVA cells, inhibit the growth of MC38-OVA
tumor cells, and overexpress SERTAD1 CD8. + The tumor volume and weight in the T-
cell adoptive group were significantly smaller than those in the control group.