99results about How to "High-throughput detection" patented technology

The present invention recognizes that the determination of ion transport function or properties using direct detection methods, such as whole cell recording or single channel recording, are preferable to methods that utilize indirect detection methods, such as FRET based detection system. The present invention provides biochips and other fluidic components and methods of use that allow for the direct analysis of ion transport function or properties using microfabricated structures that can allow for automated detection of ion transport function or properties. These biochips and fluidic components and methods of use thereof are particularly appropriate for automating the detection of ion transport function or properties, particularly for screening purposes.

The invention discloses a transcription factor detection method based on DNA-Ag nanoclusters molecular beacons and exonuclease III cycle signal amplification. The novel nanomaterial DNA-Ag nanoclusters molecular beacon is applied as a fluorescent probe to transcription factor detection for the first time. Also, based on the characteristic that fluorescence adjustment of DNA-Ag nanoclusters molecular beacons is carried out by an enzyme cleavable DNA fragment, exonuclease III is added to participate in signal transformation and also participate in enhancement of the detection signal. The transcription factor detection method provided by the invention has the advantages of no label, high selectivity, low cost and high sensitivity, and can realize high throughput detection of transcription factors in biological samples.

A method and composition for the identification of biomolecule in a sample are disclosed. The method comprises obtaining a coated capture membrane stack comprising a plurality of capture membranes with each capture membrane coated with a different peptide. The membrane stack is exposed to a sample, and, after a given amount of time for the sample to permeate the membrane stack, the membrane stack is removed from the sample carrier and the capture membrane to which the biomolecule adheres is identified.

The invention relates to the technical field of food detection, and discloses a primer combination, a method and a kit for synchronously detecting 14 animal-derived components in meat or meat products. According to the present invention, whether meat or meat products contain the components derived from 14 animals such as pigs, cows, sheep, goats, chickens, ducks, dogs, foxes, raccoon dogs, cats, rats, donkeys, deer and horses is identified by using 10 pairs of specific primers based on two 5-plex PCR reactions and a microchip electrophoresis technology, and the sequences of the 10 pairs of thespecific primers are represented by SEQ ID NO.1-20; and the method has advantages of accurate detection, high sensitivity, strong specificity, simpleness, rapidness and high efficiency, can meet therequirements in the large-batch and rapid identification of the presence of the components derived from 14 animals such as pigs, cows, sheep, goats, chickens, ducks, dogs, foxes, raccoon dogs, cats, rats, donkeys, deer and horses in meat or meat products, can effectively resist the adulteration of meat products and effectively protect the interests of consumers, and has good social benefits.

The invention relates to a recombinant protein and test strip for detection of antibodies of 2019 novel coronavirus through a double-antigen sandwich method and a preparation method and application ofthe recombinant protein and test strip, and belongs to the technical field of virus detection. The amino acid sequence of the recombinant protein for detection of the antibodies of the 2019 novel coronavirus through the double-antigen sandwich method is shown in SEQ ID NO. 1. The recombinant protein is a fusion protein of multiple dominant epitopes of the 2019-nCoV, a reagent for detection of theantibodies of the 2019-nCoV through the double-antigen sandwich method can be prepared, storage at room temperature, fast and single detection with high sensitivity, high throughput and low instrument cost can be achieved at any time, the operation is simple, and convenience of clinical use can be improved greatly.

The invention relates to a high throughput test method for a tomato bacterial disease, and more particularly relates to a high throughput test method which can utilize a locking probe to carry out reverse dot blot hybridization on tomato bacterial wilt original bacteria, tomato ulcer pathogenic bacteria and a tomato spot disease, and belongs to the crop preventing and curing disease and exit and entry plant quarantine range. The high throughput test method provided by the invention is based on a rolling circle amplification technology of the locking probe, a pair of universal primers are utilized for amplifying multiple pathogens, then, the high throughput test method and a reverse dot blot are combined together, a result is judged through a chromogenic reaction, the testing time of the traditional tomato bacterial disease is shortened, the specificity and the sensitivity of the detection are guaranteed, and the high throughput test is realized. According to the method, the testing cycle of the traditional pathogenic bacteria can be shortened, the cargo clearance can be increased, the intercept and capture relevance ratio of epidemic situations of imported bulk farm-product is improved, the pest invasion capacity is improved, the cargo clearance of cargoes imported and exported at ports in China is accelerated, and the enterprise cost is reduced and the high throughput test method has the important meanings.

The invention discloses a method for detecting multiple targets based on nucleic acid aptamer fluorescence sensor, which applies polypyrrole nanoparticles and DNA silver nanoclusters for the first time and establishes an aptamer sensing platform based on a steric hindrance effect.In addition, the invention obtains a good detection result based on the low non-specific adsorption characteristic ofthe polypyrrole nanoparticles, and realizes the detection of multiple targets based on the specific recognition characteristic of the aptamer sequence, improving the universal performance of the invention.The method for detecting multiple targets based on nucleic acid aptamer fluorescence sensor, provided by the invention, has the advantages of label-free property, no enzyme and high sensitivity,and can realize high-efficiency detection in biological samples.

The invention provides a recombinant protein and test paper for detecting a novel coronavirus 2019-nCoV IgA antibody, and a preparation method and application, and belongs to the technical field of virus detection. The amino acid sequence of the recombinant protein is shown in SEQ ID No.1; the test paper comprises a bottom plate as well as a sample absorption pad, a fluorescent microsphere pad, anitrocellulose membrane and a water absorption pad which are in sequential lap joint and adhesion to the bottom plate; a 2019 novel coronavirus specific antigen marked by fluorescent microspheres is sprayed to the fluorescent microsphere pad; a detection zone and a quality control zone are fixed on the nitrocellulose membrane; an anti-human IgA antibody is sprayed to the detection zone; and a sheep anti-chicken IgY antibody is sprayed to the quality control zone. By detecting the IgA antibody in a saliva sample, the test paper provided by the invention is capable of simply, rapidly and accurate detecting novel coronaviruses, and early-stage detection on the novel coronaviruses can be achieved.

The invention discloses a detection kit and a detection method for 3 species of food-borne viruses in marine products. The kit comprises reverse transcription Tag DNA polymerase with a concentration of 5U / mu L, inverse transcriptase with a concentration of 5U / muL and an RT-PCR reaction solution, wherein the RT-PCR reaction solution contains 10 millimols of Tris.HCl, 50 millimols of KCl, 25 millimols of MgCl2, 10 millimols of dNTP, a ribonuclease inhibitor with a concentration of 40 U / mu L, and 20 mu mols of downstream primer pair and 20 mu mols of upstream primer pairs of the food-borne viruses. The kit can synchronously detect hepatitis A viruses, rotaviruses and norwalk viruses. The kit has extremely high sensitivity, can detect 1*10 nano gram of viral nuclei and is superior to the prior reported detection method for viruses in the marine products. In addition, the method is short in detection time, simple and quick, and suitable for quick detection.

The invention relates to the technical field of gene polymorphism detection, in particular to a detection method of yak FOXO1 gene single nucleotide polymorphism and a kit thereof. The detection method comprises the following steps: 1) preparing a yak FOXO1 gene amplification product; 2) synthesizing a high and low temperature interior label; 3) preparing an HRM-PCR (High Resolution Melt-Polymerase Chain Reaction) amplification product; 4) collecting a fluorescence signal. Compared with an HRM method used for detecting polymorphic sites in the prior art, a gene probe designed by the detection method is more accurate and flexible in positioning, is accurate in parting, does not need PCR post-processing, has high working efficiency and good repeatability and is low in sample quality and quantity requirements, especially, the concentration requirement of a DNA template can be lowered to 0.1ng / mu L, and time and labor are saved when a sample size is large. Meanwhile, the detection kit has the advantages of high sensitiveness, high detection speed and good stability.

The invention provides a method for identifying five dermatophytes by utilizing a high-resolution melting curve and belongs to the technical field of high-resolution melting curve analysis. The methodadopts a pair of specific primers and is based on real-time fluorescence PCR melting curve to identify trichophyton rubrum, trichophyton digitorum, epidermophyton floccosum, microsporum caninum and Microsporum incurvatum. The sequence of the pair of specific primers is as shown as SEQ ID NO.1-2. The method has the advantages of high sensitivity, good specificity and high detection speed, can be used for identifying dermatophytes on the aspects of clinical diagnosis, environmental monitoring, food safety and the like, and provides a reliable basis for infection treatment, environmental sanitation and food safety monitoring of dermatophytes.

The invention provides an immunoquantitation analyzer which mainly consists of the following components: a reagent card moving mechanism, a dosing device, a detection structure, a liquid path system,a waste cabin mechanism and a rack structure. By adopting the immunoquantitation analyzer provided by the invention, effects of high-flux detection, high detection precision and stable and reliable detection data can be achieved, and in addition, the analyzer is simple in overall structure and small in size.

The invention discloses an oligonucleotide gene chip and application of the oligonucleotide gene chip to the detection of various bacteria. Probes of the oligonucleotide gene chip can be hybridized with polymerase chain reaction (PCR) amplification products of M. tuberculosis, M. bovis, M. avium, M. paratuberculosis and Brucella respectively; and the probes have nucleotide sequences shown as SEQ ID No:25, SEQ ID No:31, SEQ ID No:35, SEQ ID No:37 and SEQ ID No:39 respectively. The gene chip is applied to the detection of the bacteria and has high repeatability, and interassay and intraassay coefficients of variation (CV) are less than 15 percent. The gene chip shows high accuracy on the detection of a pathogenic bacterium sample to be detected, which indicates that the method has high specificity and sensitivity; the high-throughput and parallel detection is realized; detection results are quick and accurate; the whole process from the preparation of nucleic acid to the finishing of the detection only needs 6 to 8h; and the gene chip has broad prospect in preparing reagents and products for pathogen detection, import and export quarantine and epidemiological analysis.

The invention discloses a method for analyzing tar-induced cell DNA (deoxyribonucleic acid) damage on the basis of quantitative analysis of the high content technology. The method includes the steps of 1), cell culture; 2), cell contamination; 3), immunofluorescent labeling of gamma H2AX; 4), quantitative analysis of high content technology. The method has the advantages that automatic imaging is performed by the aid of a high content imaging system, tar-induced gamma H2AX protein of DNA double-strand fracture markers is quantitatively analyzed, cells are detected directly and rapidly, and sample processing is facilitated; distribution of gamma H2AX in cell nucleuses can be observed directly and image materials are stored and reanalyzed conveniently through high-resolution imaging, the tar-induced gamma H2AX in each cell nucleus can be quantitatively analyzed, and detection results are more sensitive and accurate.

The invention discloses a microfluidic chip based on a nanometer drug delivery system screening 3D solid tumor model and a preparation method and the application thereof. The microfluidic chip consists of a substrate and a cover plate which are bonded together, wherein the cover plate structurally comprises three parallel microchannels, which are mutually connected and include a microchannel (1) for drug infusion and monolayer angiogenesis, an extracellular matrix connecting channel (2), and a microchannel (4) formed by three-dimensional tumor multicellular spheres and having many U-shaped groove structures (3); the substrate has a parallel channel (5) which facilitates cell capture. The microfluidic chip can achieve real-time monitoring of targeted delivery of the nanometer drug delivery system and online evaluation of drug toxicity at the same time, so that the drug evaluating efficiency is greatly improved; in addition, the microfluidic chip has better tumor simulating capability and provides more accurate data for preclinical drug evaluation; furthermore, the microfluidic chip platform has the advantages of small reagent dosage, low cost, simultaneous analysis of a large number of samples and the like, and has a relatively good drug screening application prospect.

The invention discloses a probe for detecting expression abundance of circular RNA. The probe includes backsplicing sites at the 5' terminal of a donor exon and at the 3' terminal of an accepter exon of the circular RNA. The invention also discloses a gene chip containing the probe, and a method for detecting the expression abundance of the circular RNA by virtue of the gene chip, wherein the method mainly comprises the following steps: 1) extracting and purifying total RNA of a to-be-detected sample; 2) removing ribosome RNA; 3) enriching circular RNA; 4) conducting linear amplification to the enriched circular RNA and labeling fluorescence; 5) hybridizing a fluorescence-labeled product with a chip probe; and 6) washing and scanning the chip, and analyzing data. By designing the probe and the gene chip which are capable of achieving specific detection of the expression abundance of the circular RNA, the rapid and high-throughput detection of the expression abundance of the circular RNA can be achieved, so that shortcomings in an existing circular RNA detection technology are overcome.

The invention provides an enzyme immunoassay method for heavy metal chromium detection and an enzyme immunoassay detection reagent kit. Colloidal gold particles are coupled with horse radish peroxidase and anti-chromium monoclonal antibodies for forming enzyme marker compounds, and a detection method is built; through preprocessing chromium in samples and detecting original competitive enzyme-linked labelled antibody compounds, the detection on the chromium content in the samples to be tested is realized through chromogenic reaction amplification signals. The enzyme immunoassay method for chromium detection belongs to a one-step process competitive enzyme-linked immunoassay method, and belongs to a simple convenient fast and high-flux detection method.

The invention discloses a kit for detecting polymorphism of non-small cell lung cancer mononucleotide. The kit comprises PCR reaction reagents, specific primers and single-base extension primers, wherein the specific primers and the single-base extension primers are used for genetic locus SNP detection, and genetic loci comprise the CBR1 genetic locus rs3787728, the TP63 genetic locus rs7631358 and the CIR1 genetic locus rs13009079. The reaction system can detect multiple genetic polymorphic loci, decrease the reaction frequency, increase the detection throughput and reduce usage of samples and various consumables; reagents such as fluorescent dye and special enzymes which are high in price are not needed, and the cost is low; the accuracy is 99.99%, the sensitivity is 5 ng of genome DNA, and the kit can accurately detect out polymorphic loci of multiple genes and has the advantages of being high in throughput, automatic, low in cost and the like.

The invention relates to the technical field of bioengineering and discloses a VEGF (vascular endothelial growth factor) monoclonal antibody and a fracture healing evaluation antibody chip with the same. The amino acid sequence of a heavy chain variable region of the monoclonal antibody is shown as SEQ ID NO.1, and the amino acid sequence of a light chain variable region of the monoclonal antibody is shown as SEQ ID NO.2. Homogeneity and biological activity unicity of the monoclonal antibody enables antigen antibody reaction results to be convenient for quality control and beneficial to standardization and normalization. The chip with the monoclonal antibody can be directly applied to clinical therapeutic evaluation on fracture healing, solves the technical problems of time and labor consumption, poor result repeatability and the like of the prior art, has extremely high application value in the field of clinical treatment and has wide application prospect.

Multi-capillary systems for high-throughput electrophoretic separation and detection of biomolecules are disclosed. One embodiment of the invention uses galactomannans as a size-sieving matrix for multi-channel electrophoretic separations of biomolecules. Multi-color detection for the simultaneous analysis of controls and standards in the same channels as the samples, and endogenous fluorescence detection are also disclosed. Another embodiment of the invention is a two dimensional system for separation of complex samples, using multiplexed capillary electrophoresis system as the second dimension, with a fraction collection step connecting the two separation steps. The systems allow for separations to be accomplished with a highly parallel manner, or in a two-dimensional format.

The invention relates to a detection method for BRAF gene mutation, comprising the following steps: a) extracting DNA of tumor tissue; b) with the extracted DNA in step a) as the template, carrying out PCR amplification by using a primer pair comprising SEQ (1) and SEQ (2) in a sequence table and Taqman-MGB probes of SEQ (3) and SEQ (4); c) carrying out BRAF allele identification and analysis on products of the amplification in step b). The detection method provided in the invention can assist clinicians in detecting BRAF gene mutation of patients and provide guidance and basis for clinicians to use medicines, and therefore risk in treatment for clinicians and economic burden for patients are reduced. According to the invention, high flux detection is realized and up to 96 samples can be detected at a time; the detection method has a simple process, thereby substantially raising detection accuracy; detection time is short; wild type and mutant alleles can be detected simultaneously; and high sensitivity and good specificity are obtained.

The invention provides an electrochemical detection device based on a self-assembling technology and a micro-fluidic chip technology. A sample injection system comprises a sample feeder and a micro-fluidic chip. The micro-fluidic chip comprises a sample inlet, and a sample channel, an antibody channel and a detection liquid channel which are communicated with the sample inlet. Control valves are arranged on the sample channel, the antibody channel and the detection liquid channel, and the sample feeder is connected with the sample inlet. Storage systems are respectively arranged on the antibody channel and the detection liquid channel. Driving systems are arranged on the storage systems. A detection system comprises an electrode group and an electrochemical detector connected with the electrode group, and the micro-fluidic chip is connected with the electrode group. A working electrode in the electrode group is coated with a first antibody corresponding to a sample, a freeze-dried second antibody corresponding to the sample is placed in the antibody channel, and the storage systems respectively store a second antibody dissolving solution and a detection solution. The device reducesuse of human resources, reduces the detection cost, greatly increases the detection efficiency, and realizes high-throughput detection.

The invention discloses a probe, a kit and a method for cascade amplification testing of miRNAs based on a coding suspension microchip. The method comprises the following steps: with a target miRNAs as a template, performing a connection reaction on the template, a padlock probe and an RNA ligase in a connecting buffer solution to realize cyclization of the padlock probe; mixing the padlock probe,cyclized through connection, with a rolling circle amplification primer, a polymerase, an endonuclease and other components required by isothermal cascade amplification, and performing an isothermalcascade amplification reaction; performing a base stacking hybridization reaction on a product obtained through an isothermal cascade amplification reaction, a coding suspension microchip coupled witha capture probe, and a generalized label in a hybridization buffer solution; and after the base stacking hybridization reaction is completed, observing the coding suspension microchip by an optical detection device to realize detection of the target miRNAs. The probe, the kit and the method can realize low-cost, high-sensitivity and high-throughput detection of detected samples.

The invention provides a method for identifying four fusaria, and belongs to the technical field of analysis of high-resolution melting curves. According to the method, a pair of specific primers areadopted, fusarium proliferatum, fusarium oxysporum, fusarium semitectum and fusarium solani are identified based on a real-time fluorescence PCR (RT-PCR) melting curve, and the sequence of the specific primers is shown in SEQ ID NO.1-2. The method is high in sensitivity, good in specificity and high in detection speed and can be used for identification of fusarium strains in the aspects of clinical diagnosis, agricultural production, food safety and the like, and therefore a reliable basis is provided for monitoring of fusaria in the aspects of infection treatment, agricultural production andfood safety.

The invention belongs to the field of analysis and detection, and relates to a transcription factor fluorescence detection of based on DNA-silver nanocluster allosteric probe. The method includes a) using hairpin DNA as template, adding silver nitrate and sodium borohydride, and reducing that silver nitrate and sodium borohydride to generate DNA-silver nanocluster allosteric probe AgSwitch in situ; B) incubating a plurality of transcription factor solutions of different concentrations with AgSwitch obtained in step a); C) measuring the fluorescence value of the system, obtaining a linear relationship between the concentration of the transcription factor and the fluorescence intensity, and drawing a standard curve; D) measuring fluorescence intensity of the sample to be tested under the same conditions as the step c), and obtaining the concentration of transcription factors in the sample to be tested according to the standard curve of the step c). The method has the advantages of label-free, enzyme-free and high sensitivity, and can realize efficient detection of the transcription factors in the biological sample.

A method and related microfluidic chip and kit for high throughput detection of proteins of interest contained in a sample is disclosed. The method comprises of specifically labeling fusion proteins in a complex sample with fusion tag specific fluorophores that specifically bind the fusion tags coupled to the proteins of interest, and subjecting the sample to automated capillary electrophoresis, wherein the presence of the proteins of interest in the sample is detected by fluorescence signals associated with the fusion tag specific fluorophores.

The invention discloses a cadmium ion sensing method combining an inducible allosteric probe with rolling circle amplification. The method can realize high-sensitivity and specific detection of tracecadmium ions. Potential heavy metal pollution in air, water and soil exists everywhere, and life quality and body health of people are seriously affected. Heavy metal ion cadmium is a common heavy metal in grain pollution. Cadmium is easy to accumulate in kidneys, bone tissues, eyes and other organs and tissues of a human body. Particularly an accumulation phenomenon in the kidneys and the bone tissues is the most serious, diseases such as renal failure and fragile fractures can be caused, and human body health is threatened. The trace cadmium ions can cause serious damage to the human body, the method is of great significance to environmental protection and food safety.

The invention discloses a colorimetric method for detecting acid phosphatase on the basis of mimic biomimetic oxidase activity of manganese dioxide. The colorimetric method for detecting the acid phosphatase on the basis of the mimic biomimetic oxidase activity of the manganese dioxide comprises the following steps: producing ACP solutions and an AAP solution by adopting a NaAc-HAc buffer solution, and mixing the multiple ACP solutions with different concentrations, the AAP solution, MnO2 nanosheets and ABTS; measuring ultraviolet absorbancy degrees A and A0 at the wavelength of 420 nm to obtain a difference value deltaA of the absorbancy degrees, so as to obtain a linear relation between the ACP concentrations and the difference value deltaA of the absorbancy degrees; and calculating a concentration of the ACP of a to-be-detected sample according to the linear relation and the difference value deltaA of the absorbancy degrees of the to-be-detected sample. A colorimetric method for detecting an organophosphorus pesticide on the basis of the mimic biomimetic oxidase activity of the manganese dioxide is further disclosed; and the organophosphorus pesticide is added on the basis of the colorimetric method for detecting the acid phosphatase. According to the colorimetric method for detecting the acid phosphatase on the basis of the mimic biomimetic oxidase activity of the manganesedioxide and the colorimetric method for detecting the organophosphorus pesticide on the basis of the mimic biomimetic oxidase activity of the manganese dioxide, color comparison is conducted on the acid phosphatase and the organophosphorus pesticide, and the colorimetric method for detecting the acid phosphatase on the basis of the mimic biomimetic oxidase activity of the manganese dioxide and the colorimetric method for detecting the organophosphorus pesticide on the basis of the mimic biomimetic oxidase activity of the manganese dioxide have the advantages of being simple, high in sensitivity, low in cost and high in detection throughput.

The invention discloses a quantitative enzyme-linked immunoabsorbent assay method based on moving reaction boundary electrophoresis titration. ELISA-MARB-ET is carried out on known standard samples with different antigen concentrations respectively under a same condition, so that a mathematical model of the moving distance and antigen concentration of a moving reaction boundary is established, and an antigen concentration ruler is labeled along an electrophoresis channel; ELISA-MRB-ET is carried out on samples to be detected under the same condition in a test stage, so that a measured concentration scale corresponding to a stopping position of the moving reaction boundary is the antigen concentration in the sample. The method is reasonable in design, and quantitative detection can be realized without expensive instruments, so that the cost is reduced, and high-throughput detection can be realized.

The invention discloses a method for detecting polymorphism of a TREM2 gene, wherein the method includes the following steps: determining a mutation site of the TREM2 gene; designing and synthesizinga probe and primers according to the mutation site; mixing the detected TREM2 target gene, the probe, the primers and enzymes, carrying out fluorescence quantitative PCR, and monitoring a fluorescencesignal; and calculating the Tm value displayed by hybrid peaks, and judging the type of the TREM2 gene according to the Tm value. The invention also discloses the probe for detecting the polymorphismof the TREM2 gene, and the probe has the nucleotide sequence including SEQ ID NO.3. The invention provides the probe and method for simple operation and rapid detection of the polymorphism of the TREM2 gene.