49 results about "BRAF Gene Mutation" patented technology

The invention discloses a nucleic acid probe, a liquid chip and a detection method that are used for the mutation detection of a BRAF gene exon 15. The liquid chip that is used for the mutation detection of the BRAF gene exon 15 comprises microspheres and a primer; wherein, the microspheres are coated with wild and mutant probes which are decorated with amino groups and aim at the BRAF gene exon 15; and the primer is used for augmenting a target sequence that is enriched in mutant sites needing to be detected by the BRAF gene exon 15 and has a biotin marker in the tail end. The detection method that is provided by the invention has quickness, accuracy, simple and convenient operation, and greatly improves the detection efficiency.

The invention belongs to the field of biotechnology, and specifically relates to a combination of primers for thyroid cancer detection, including DNA PCR primers and RNA PCR primers. The DNA PCR primers include ATM gene mutation detection primer pairs, BRAF gene mutation detection primer pairs, and HRAS gene mutation detection primers. Detection primer pair, NRAS gene mutation detection primer pair, PTEN gene mutation detection primer pair, RET gene mutation detection primer pair, TERT gene mutation detection primer pair, TG gene mutation detection primer pair, TP53 gene mutation detection primer pair, TSHR gene mutation detection Primer pair, TTN gene mutation detection primer pair; said RNA PCR primers include RET gene fusion detection primer pair, NTRK3 gene fusion detection primer pair, BRAF gene fusion detection primer pair. The invention can simultaneously detect DNA mutation and RNA fusion. The amount of sequencing data for a single sample detection is reduced to less than 0.2M reads, which greatly reduces the cost of sequencing. Since DNA mutation and RNA fusion are tested in the same system, the workload of operation is reduced.

The invention provides crRNAs for gene mutation of lung cancer-related molecular markers, a detection kit and a detection method thereof. The crRNAs comprise: a crRNA at the EGFR-T790M mutation site:as shown in any one of SEQ ID NO.16-17; a crRNA at the EGFR-L858R mutation site: as shown in any one of SEQ ID NO.18-19; a crRNA at the BRAF gene mutation site: as shown in any one of SEQ ID NO.20-21;a crRNA at the ALK gene mutation site: as shown in any one of SEQ ID NO.22-23; and a crRNA at the ROS1 gene mutation site: as shown in any one of SEQ ID NO.24-25. According to the invention, the 5 crRRNAs related to gene mutation targets of lung cancer-related molecular markers are designed, and the crRNAs are adopted in combination with a CRISPR-cpf1 system to carry out mutation detection. The method of the invention has the characteristics of simplicity, fast speed, high sensitivity and strong specificity.

The invention discloses application of a BRAF gene detector to preparation of an ACTH type pituitary adenoma molecular pathological diagnosis and parting product. It is proved through tests that BRAF gene mutation is peculiar to ACTH type pituitary adenoma and not found in other types of pituitary adenoma, so that the BRAF gene mutation can serve as a specific molecular pathological diagnosis marker of the ACTH type pituitary adenoma. According to the result that a BRAF gene is mutant or not, the ACTH pituitary adenoma can be further divided into two subtypes, namely the BRAF mutant type and the wild type, the mean volume of the BRAF mutant type ACTH pituitary adenoma is small, the attack degree is low, and the midnight serum cortisol concentration is less than or equal to 22 ug / dl; the mean volume of the wild type ACTH pituitary adenoma is large, the attack degree is high, and the midnight serum cortisol concentration is greater than 22 ug / dl. Therefore, the different subtypes of the ACTH pituitary adenoma can be judged by detecting whether the BRAF gene is mutant or not, and different clinical treatment strategies are adopted in time accordingly.

The invention discloses a reagent storage device for detecting BRAF gene mutation. In the invention, the cooling liquid is used as the temperature maintaining medium of the reagent tube, which can effectively improve the temperature maintaining stability of the reagent tube. The cooling liquid in the liquid exchange and cooling components is monitored in real time. When the liquid is exchanged synchronously, the cooling liquid in the circulating pipeline can be supplemented into the liquid exchange tank by the suction of the micro suction pump, and the Part of the cooling liquid in the liquid exchange tank is discharged, so that the cooling capacity of the cooling liquid in the liquid exchange tank can be smoothly improved to reach the initial level. Push the push plate to move, the push plate can push out the liquid in the liquid exchange tank, and the distance between the top of the push plate and the top of the liquid exchange tank can ensure that the push plate is automatically reset after the liquid exchange is completed, which improves the liquid exchange rate. Efficiency ensures the effectiveness of the reagents, thereby ensuring the accuracy of the detection.