KRAS, NRAS and BRAF gene mutation detection kit

A detection kit and mutation detection technology, applied in the field of medical detection, can solve the problems of high cost, short detection time, cumbersome operation, etc., and achieve the effects of convenient operation, high cost performance and less manual operation.

Inactive Publication Date: 2018-01-16
SHENZHEN YOU SHENGKANG BIOSCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cost of instruments and reagents for this method is high, the operation is cumbersome, and the throughput is limited, so it is difficult to promote it in the market
[0007] In addition, conventional ARMS-PCR technology is easy to operate, the detection time is short, and the detection sensitivity is generally about 1%. When detecting blood samples, the sensitivity is low, and the detection rate is significantly lower than the corresponding tumor tissue samples. less quantitative than sequencing methods
Sanger sequencing technology has high detection specificity and throughput, but the method itself is not sensitiv

Method used

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  • KRAS, NRAS and BRAF gene mutation detection kit
  • KRAS, NRAS and BRAF gene mutation detection kit
  • KRAS, NRAS and BRAF gene mutation detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0111] 1) Primer sequence design

[0112]

[0113]

[0114] 2) Sample preparation

[0115] Use the FFPE DNA extraction kit to extract paraffin-embedded tissue sample sections, and dilute the extracted DNA to 2ng / ul with TE.

[0116] 3) Instrument

[0117] ABI7500, nanodrop2000, high-speed centrifuge, low-speed centrifuge, constant temperature dry bath, vortex oscillator, refrigerator.

[0118] 4) Composition of the kit

[0119] The kit consists of KBN 8-linked PCR reaction strips and KBN positive control.

[0120] The basic composition of each well of the reaction strip is: 1×PCRBuffer, 2~8pmoldNTPs, 1.5~4.0mMMgCl 2 , 1×SybrGreenI, 0.5~2UHSaq, 0.1~1UUNG enzyme. According to Table 1), each primer probe was sequentially added to the 8-tube strip, and the final concentration was 100-400 nM.

[0121] 5) Detection process

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PUM

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Abstract

The invention provides a KRAS, NRAS and BRAF gene mutation detection kit, and aims to overcome the defects of an existing kit on the aspects of sensitivity, cost, flux, convenience in operation and the like. The detection kit comprises a detection reagent, wherein the detection reagent consists of an internal control reagent, a mutation detection reagent and an external control reagent. The detection kit has the beneficial technical effects of high sensitivity, high flux, wide sample application range, low cost and convenience in operation.

Description

technical field [0001] The invention belongs to the technical field of medical detection, and in particular relates to a DNA detection material, specifically a KRAS, NRAS, BRAF gene mutation detection kit. The product of the invention is suitable for detecting KRAS / NRAS / BRAF gene mutations of human tumors. Background technique [0002] Studies have shown that cancer is the result of the interaction between environmental factors and cell genetic material, the result of multi-factor, multi-stage and multi-gene effects, and the result of the accumulation of gene mutations, so cancer is a genetic disease. Among the many human genes, proto-oncogenes and tumor suppressor genes are closely related to the occurrence and development of cancer. Mutations in proto-oncogenes and / or tumor suppressor genes can cause cells to become cancerous. Before cancer forms a solid mass, the process of cancer-related gene mutation has already occurred, and this process may have lasted for more than...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/11
CPCC12N15/11C12Q1/6886
Inventor 喻德华翟建新罗玄李鑫剩
Owner SHENZHEN YOU SHENGKANG BIOSCI CO LTD
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