134 results about "Tumor targeted" patented technology

The invention belongs to the technical field of nuclear medicine molecular diagnosis and treatment, and discloses a modified CAIX targeting cyclic peptide, a nuclide marker and application of the modified CAIX targeting cyclic peptide in tumor diagnosis and treatment. According to the CAIX targeted cyclic peptide, a rigid bifunctional chelating agent RESCA is introduced to replace a traditional chelating agent, and a sulfonated or alkylated amino acid connector is combined, so that the enzymolysis resistance and in-vivo stability of the probe are remarkably improved. By optimizing the structure of the connector, the lipophilicity is reduced, liver and gall metabolism and non-specific uptake are reduced, and the high uptake rate of tumor target tissues is maintained. The therapeutic nuclide marker of the cyclopeptide can be used for intratumoral irradiation therapy using DOTA or NOTA in combination with an alkylated amino acid linker or an albumin ligand. Experiments show that the marker has high radiochemical purity, excellent pharmacokinetic characteristics and low kidney retention, and is suitable for precise diagnosis and targeted therapy of CAIX high expression tumors such as kidney cancer, colorectal cancer and brain glioma.

The invention relates to a tumor target volume segmentation method and device and a storage medium. The method comprises the following steps: acquiring a medical image containing a target tumor; segmenting the target tumor in a spatial dimension according to the medical image to obtain a plurality of first target volumes which are located in the target tumor and are not overlapped; grouping the plurality of first target volumes to obtain a plurality of target volume groups; wherein each target volume group comprises at least one first target volume, and the adjacent first target volumes in the plurality of first target volumes are located in different target volume groups. According to the tumor target volume segmentation method and device and the storage medium provided by the invention, continuous irradiation of adjacent areas can be avoided through differential irradiation of different target volume groups, so that accumulated damage to surrounding normal tissues is reduced.

The invention relates to the technical field of medical nano-materials, and discloses a pH-responsive drug self-delivery nano-system and a preparation method and application thereof.The preparation method comprises the steps that 1, CMNPs is prepared, specifically, through an amidation reaction, the CMNPs are prepared from methotrexate and cis-aconitic anhydride; step 2, synthesizing DSPE-PEG-T12: synthesizing the DSPE-PEG-T12 by virtue of a nucleophilic addition reaction; step 3, preparing a T12 peptide modified erythrocyte membrane; and step 4, preparing T12-RBCM (at) CMNPs: modifying the T12 peptide of the targeted tumor cell transferrin receptor on an erythrocyte membrane, and coating the CMNPs to construct the nano-acquisition system T12-RBCM (at) CMNPs. According to the nano system T12-RBCM (at) CMNPs obtained by the scheme, the tumor cell uptake efficiency can be remarkably improved, cell apoptosis is induced, and proliferation is inhibited; the long-acting circulation and tumor targeting capability is realized, the tumor growth can be effectively inhibited, and the biological safety is good.

The invention relates to the technical field of image processing, in particular to a small intestine capsule endoscope target detection method which comprises the following steps: acquiring a small intestine capsule endoscope image; the method comprises the following steps: constructing an improved RT-DETR network, inserting an SCSA module between a backbone network and a neck network of the RT-DETR, and introducing a DFM module behind a three-branch Fusion module of the neck network; and the improved RT-DETR network adopts a Focaler-IoU loss function. According to the method, the problem that the accuracy of recognizing the tumor target by using the small intestine capsule endoscope image through RT-DETR needs to be further improved is solved.

The application provides a preparation method of a Chlorella-arsene composite drug delivery system and application thereof. The application prepares arsene nanosheets (ANs) through a liquid phase stripping method, and obtains catechol-polyethyleneimine nanoparticles (CA-PEI) through oxidation self-polymerization. The ANs and the CA-PEI are charge-absorbed to form CA-PEI loaded with ANs (CA-PEI@ANs), and then charge-absorbed with protein nucleus Chlorella (CP), so that a Chlorella-arsene composite drug delivery system (CP@CA-PEI@ANs) is successfully constructed. The preparation operation of the drug delivery system is simple, and the reproducibility is good, and the drug delivery system has a good application prospect. The protein nucleus Chlorella is used as a drug delivery carrier, so that the problems of high cost and complex synthesis process commonly existing in a targeted delivery carrier are solved, and the application has good tumor targeting effect, and is beneficial to better drug efficacy.

The present application relates to the field of immunology, and particularly relates to a novel IL-15 fusion protein and application thereof. The present application provides an IL-15 transmembrane fusion protein, which is applied to NK cells or CAR-NK cells, can significantly enhance the tumor killing function of NK cells and CAR-NK cells, and can effectively promote the proliferation of NK cells and CAR-NK cells. It provides an important basis for developing NK cell products armored by the IL-15 transmembrane fusion protein, which are independent of any tumor target, thereby reducing the cycle and cost of CAR-NK development. Meanwhile, the present application respectively carries out function research in blood tumors expressing CD19 and solid tumors expressing CD276, and the results indicate the broad-spectrum application prospect of the structure in tumors.

This invention discloses a KK-LC-1 targeting binding protein and its derivatives, a kit, and applications. The protein sequence of the KK-LC-1 targeting binding protein is shown in one of SEQ ID No. 2, SEQ ID No. 3, SEQ ID No. 4, and SEQ ID No. 5. The KK-LC-1 targeting binding protein of this invention has a molecular weight of only about 17 kDa, approximately one-tenth that of an antibody, exhibiting good tissue penetration. Furthermore, its production process is simple and yields high quantities. The targeting binding protein of this invention demonstrates good targeting activity against KK-LC-1-positive tumors both in vivo and in vitro, and can be used for targeted tumor drug delivery, representing a potential drug for tumor immunotherapy. Simultaneously, a KK-LC-1 serum detection kit was developed using the targeting binding protein, enabling quantitative detection of KK-LC-1 levels in the human body.

Provided is a bispecific antibody targeting CD3 and a tumor target and a use of the bispecific antibody. The bispecific antibody comprises a first antigen binding region, a second antigen binding region, and an Fc portion; the Fc portion comprises a first Fc peptide fragment and a second Fc peptide fragment; the first antigen binding region comprises an scFv fragment and a binding protein or fragment thereof of a first molecule, the binding protein or fragment thereof of the first molecule is linked to the N-terminal of the scFv fragment, and the C-terminal of the scFv fragment is linked to the N-terminal of the first Fc peptide fragment; the second antigen binding region comprises a binding protein or fragment thereof of a second molecule; and the first molecule and the second molecule are the identical, and the scFv fragment has CD3 binding activity.

The application belongs to the field of pharmacy, and particularly relates to a whole-process targeting polypeptide molecule with the functions of targeting brain capillary endothelial cells, tumor neovascular endothelial cells, tumor pseudo vascular, tumor cells and tumor stem cells, a stable and optimized polypeptide molecule, and a modified complex and drug delivery system for tumor diagnosis and treatment. The application prepares a whole-process targeting polypeptide WVAP and a WVAP modified drug and high polymer carrier material, and applies the WVAP to the construction of a drug delivery system for tumor imaging and targeted therapy. The results show that the WVAP can cross the blood-brain barrier, target tumor neovascular and cross the blood-tumor barrier, target tumor pseudo vascular, tumor cells and tumor stem cells; and the nano drug delivery system constructed by the WVAP modified high polymer carrier material can effectively deliver the loaded drug to the brain, target tumor tissues, and significantly improve the anti-tumor efficacy.

This application provides a method for screening inhibitors targeting CYP7A1 and their use in the preparation of anti-liver cancer drugs, relating to the field of tumor targeted drug design technology. The method for screening inhibitors targeting CYP7A1 protein includes: using the crystal structure of human cholesterol 7α-hydroxylase as a receptor model, precise docking screening is performed using software to screen drug-like molecules with molecular weights of 250-500 Da and lipid-water partition coefficients of -1 to 5 from a compound database; a graph neural network active learning model is used to predict and rank the protein-small molecule binding affinity of compounds in the database; the ΔG between candidate molecules and CYP7A1 is calculated using molecular mechanics / generalized Born surface area methods, with molecules having ΔG ≤ -40 kcal / mol considered potential inhibitors. The inhibitors of this application do not rely on the enzymatic activity of CYP7A1 to achieve anti-tumor effects and do not significantly affect cholesterol and bile acid metabolism levels.

The invention discloses a real-time positioning method for a tumor target region and a gold label implant, and belongs to the technical field of tumor localization, and the method specifically comprises the following steps: implanting a gold label in a tumor region, constructing a gold label-tumor dynamic model through CT, and obtaining a respiratory phase feature vector; realizing space matching of the two-dimensional projection and the three-dimensional model by using an improved hybrid registration algorithm, and obtaining a tumor compound displacement vector; establishing a respiration-displacement correlation model through time sequence alignment, generating a tumor movement map containing a real-time position and a predicted trajectory, and updating the reference coordinate system; establishing a rigid safety boundary based on a gold label, analyzing soft tissue texture and a prediction trajectory in combination with a convolutional neural network to generate an elastic treatment boundary, and dynamically adjusting a dose weight of a non-label area; and finally, submillimeter-level dynamic tracking is executed through a multi-leaf collimator. The problems of tumor motion uncertainty and individual deformation difference are solved, and the target area coverage precision and the dose distribution reasonability are improved.

The invention relates to the technical field of medical instrument application, in particular to a universal labyrinth source applicator and a method for realizing multi-angle accurate implantation thereof, and aims to solve the problems of insufficient focus dose coverage, high treatment cost and high efficiency caused by the fact that a bare-handed implantation or printing mold guide implantation mode is generally adopted during cervical cancer radiotherapy. And the cervical cancer radiotherapy effect is finally influenced. According to the method, function design is carried out on the implantation path of the implantation needle of the source applicator, then the source applicator is placed in a mathematical coordinate system, image data are scanned through CT equipment, the vertical distance, namely the transverse implantation range, from the tumor target position to the uterine cavity tube is calculated, and then the depth, needing to enter the tumor, of the implantation needle is calculated according to the number of image layers from the tumor target position to the lower surface of the source applicator. After the needed needle insertion channels are predicted, the multiple needle insertion channels of the labyrinth insertion source applicator are selected for insertion, so that the radioactive source can accurately cover a tumor focus treatment area during tumor after-loading treatment, and the overall treatment effect of cervical cancer is greatly improved.

The invention provides an application of 2-indolecarboxylic acid in preparation of a tumor targeted therapy drug. Through computer-aided drug design and molecular docking optimization, 2-indolecarboxylic acid shows high binding energy to an HER2 kinase structural domain. In HER2 positive NCI-N87 gastric cancer cells, the 2-indolecarboxylic acid significantly inhibits cell proliferation and induces G0 / G1 phase arrest and apoptosis. The mechanism research shows that the 2-indoleformic acid can play a role by regulating and controlling the HER2 / Akt / beta-catenin pathway (Western blot verification). In an NCI-N87 cell tumor-bearing nude mouse model, 2-indolecarboxylic acid (15 mg / kg) significantly inhibits tumor growth, and does not cause obvious weight loss or organ toxicity. Immunohistochemical analysis shows that the positive rate of a tumor tissue proliferation marker Ki-67 in a treatment group is remarkably reduced, and apoptosis detection shows that the proportion of TUNEL positive cells is remarkably increased, which indicates that 2-indolecarboxylic acid inhibits tumor cell proliferation and promotes apoptosis at the same time. The invention provides a lead compound with clinical transformation potential for HER2 targeted therapy.

The application belongs to the technical field of medicine preparation, and particularly relates to a magnetic layered double hydroxide, a targeted drug-loaded nano preparation, a preparation method and application of a tumor-targeted drug-loaded nano preparation. The preparation method of the magnetic layered double hydroxide provided by the application can prepare Fe3O4 nanoparticles with small particle size, and further prepare the magnetic layered double hydroxide which can be used as a water-soluble drug carrier. The magnetic layered double hydroxide is used for preparing the targeted drug-loaded nano preparation and the tumor-targeted drug-loaded nano preparation, and can be used for delivering water-soluble drugs such as 5-fluorouracil. The tumor-targeted drug-loaded nano preparation has good dispersity and can be actively targeted to HepG2 cells with high FR expression, has a good inhibitory effect on liver cancer cells, and is expected to provide a potential new treatment system for magnetic chemotherapy.

The invention discloses an albumin coupling medicine as well as a preparation method and application thereof. The cathepsin B response type camptothecin albumin coupling drug is innovatively designed and synthesized by taking albumin as a carrier, the albumin coupling drug has good cathepsin B response drug release capability, efficient tumor targeted delivery of the drug is realized, camptothecin is responded and released in tumor cell lysosome, and the drug delivery efficiency is improved. The specific anti-tumor effect is achieved. The invention provides a regulation and control method in the aspects of preparation, drug loading capacity and in-vitro release of the albumin coupled drug, size effects in the aspects of cellular uptake, cytotoxicity, tumor targeting and retention are illustrated, and guidance is provided for designing an efficient and safe tumor drug delivery system.

This invention discloses a method for esophageal cancer tumor target region segmentation based on cross-modal feature fusion of PET / CT images. The method uses a Transformer-fused Attention Progressive Semantic Nested Network (TransAttPSNN) as the 3D segmentation model for esophageal cancer tumor target regions. The TransAttPSNN network has an AttPSNN backbone structure and includes two segmentation networks: one for PET streams and the other for CT streams. Transformer cross-modal adaptive feature fusion modules are embedded in the different feature levels of the two segmentation networks. Compared with existing technologies, this invention effectively improves the segmentation accuracy of esophageal cancer tumor target regions and achieves better segmentation performance.

The present invention relates to the technical field of tumor tissue positioning, and specifically to a method and system for automatic delineation of tumor radiotherapy target areas based on artificial intelligence. Based on RGB images and grayscale images of tumor tissue, the present invention first performs edge detection to extract grayscale connected domains. The grayscale similarity and distribution characteristics between pixels in each connected domain are analyzed, the tumor characterization degree is calculated, the high metabolic area is screened out and its position in the RGB image is located. For the first preset area, based on the difference and distribution characteristics of the pixel B channel value and the central area, a low infiltration characteristic factor is generated and the low infiltration area is delineated. Further, in the second preset area outside the low infiltration area, the diffusion characteristic factor is calculated by combining the R / B / G channel difference of the pixel, and the gradient value is fused to obtain the diffusion area, thereby automatically delineating the tumor tissue. The present invention can accurately segment the high metabolic area, low infiltration area and diffusion area in the tumor tissue, thereby improving the accuracy of automatic delineation of the tumor target area.

The invention discloses a process for synergistically inhibiting melanoma by carrying low-dose paclitaxel on nanoparticles based on immunoregulation. According to the invention, hyaluronic acid functionalized albumin nanoparticles are combined with low-dose paclitaxel, the tumor targeting property of the drug is improved by utilizing a nanotechnology, and meanwhile, the tumor immune microenvironment is regulated and the anti-tumor immune effect is enhanced by activating an STING pathway. Through the combination, the treatment effect of the medicine is improved, the side effect of the medicine is remarkably reduced, and a new strategy is provided for tumor treatment. High-efficiency delivery of low-dose paclitaxel is realized through the nano-carrier, the drug dosage is reduced, the toxicity is reduced, meanwhile, the antitumor activity of the paclitaxel is retained, a new thought is provided for clinical application of traditional chemotherapeutic drugs, the tolerance and life quality of patients are expected to be improved, polarization of M1 type macrophages is promoted by activating an STING pathway, and the antitumor activity of the paclitaxel is improved. Interferon and other inflammatory factors are released, CD8 + T cells are recruited and activated, and therefore the anti-tumor effect is achieved.

The invention provides a nano-drug system based on active drug self-assembly, a preparation method and application, CPT-C, MTX-C, ART and a lipid material are dispersed in an organic solvent, and a CMA (at) RGD-NPs nano-drug system is prepared through a film dispersion method. By constructing a nano drug delivery system with tumor targeting, ROS response release and multi-mode synergistic treatment, endogenous ferroptosis can be induced, and toxicity introduced by exogenous iron is avoided; a cGAS-STING pathway is activated, and natural and adaptive immunity is enhanced; and in combination with immunotherapy, a forward feedback loop is formed, and growth and transfer of TNBC are synergistically inhibited.

The invention discloses a chimeric antigen receptor T cell (CAR-T) of modular artificial antigen-natural antigen logic gating (MANAGE) and application of the chimeric antigen receptor T cell in treatment of solid tumors. At present, solid tumors still lack efficient targets capable of covering tumor cells of the same focus and crossing tumor species, and most solid tumor targets are also expressed on normal tissue cells. Therefore, the application of single-target CAR-T therapy in solid tumors is limited by tumor heterogeneity and non-tumor targeted toxicity (OTOT). According to the invention, proteins and antibodies of targeted tumor cells modified by the artificial antigen FITC are constructed, and CAR-T double-gated by the FITC and the natural tumor antigen is researched and developed. When the two compounds are combined for use, the toxicity of OTOT can be reduced, the range of applicable cells and tumor species can be expanded, the injection time of FITC can be regulated and controlled, the effect of CAR-T can be'switched ', cytokine syndromes can be reduced, and a new method is provided for solid tumor treatment.

The invention relates to a multi-modal medical image deep learning enhanced segmentation system and a method thereof, belongs to the field of medical image processing, and aims to solve the problem of inaccurate brain glioma target region boundary segmentation. According to the technical scheme, the method comprises the steps that a metabolic domain PET image and a structural domain MRI-T2 image are obtained, and image registration, noise suppression and intensity standardization preprocessing are carried out; glycolysis features and texture anisotropy features are extracted through a double-domain feature decoupler; constructing a graph neural network to generate a metabolism-structure interaction graph; calculating a pathological infiltration confidence coefficient thermodynamic diagram based on the atlas; the thermodynamic diagram is used for self-adaptive radiotherapy dose segmentation, and the dose gradient is dynamically adjusted; and finally outputting the tumor target boundary.

The present disclosure provides novel radiopharmaceutical conjugates for tumor targeting and delivery, and their therapeutic and / or theragnostic use.

The invention belongs to the technical field of biological medicines, and particularly relates to sulfonium salt polypeptide or protein as well as a preparation method and application thereof. Polypeptide or protein containing cysteine and a sulfonium salt compound are used as raw materials, and the sulfonium salt compound is used for alkylation of cysteine at a specific site of the polypeptide or protein to prepare the sulfonium salt polypeptide or protein. The sulfonium salt polypeptide or protein is used as a probe for covalently labeling tryptophan in a lysine methylation reader protein recognition pocket. According to the method disclosed by the invention, different types of sulfonium salt polypeptides and protein probes can be simply, conveniently and efficiently prepared, and the designed annular sulfonium salt with a novel skeleton has an obvious improvement on the polypeptide or protein labeling yield of reader protein. The prepared sulfonium salt probe not only can be used for identifying lysine methylation modified reader protein and finding a therapeutic target for disease-related pathways, but also can be used for modifying tryptophan at a specific site of an antibody-coupled drug and provides a powerful tool for targeted therapy research of tumors.

The invention discloses a tumor treatment synergistic interaction system and method based on the acoustic cavitation effect of a phase-change nano-droplet. The system comprises the phase-change nano-droplet entrapped with a chemotherapeutic drug and a sound-sensitive agent and a cavitation effect intelligent monitoring and adjusting system. The method is characterized in that cavitation noise signals of a tumor target area are collected in real time through a cavitation monitoring system integrated in an ultrasonic probe, the inertial cavitation dosage is rapidly calculated through a signal processing system and compared with a preset threshold value, then a feedback instruction is generated to dynamically regulate and control ultrasonic irradiation parameters, and intelligent closed-loop control is formed. According to the method, the cavitation effect can be accurately maintained in an ideal treatment window, targeted release and deep penetration of the medicine and activation of the sound-sensitive agent are synchronously achieved through cavitation mechanical energy, and time-space synchronous synergistic interaction of chemotherapy and sonodynamic therapy is achieved.

The invention provides a radiotherapy plan optimization method, equipment, a system and a medium, and belongs to the technical field of radiotherapy. The method comprises the following steps: generating an initial radiotherapy plan for a tumor target region in a medical image; wherein the initial radiotherapy plan at least comprises a high-dose lattice dot matrix and a beam direction corresponding to each high-dose lattice in the high-dose lattice dot matrix; at least one of the beam direction in the initial radiotherapy plan and the rotation attitude of the high-dose lattice lattice relative to the tumor target region is optimized, so that a plurality of high-dose lattices exist in at least one beam direction, and an optimized radiotherapy plan is obtained.

The invention discloses a real-time image guiding method and system for organ motion compensation in tumor radiotherapy, and the method comprises the steps: constructing a respiratory motion model through the synchronous collection of the three-dimensional position of a gold mark and the motion track of a body surface LED mark point, and obtaining an atrophy compensation component through the combination of the volume atrophy proportion and the gravity center displacement of the gold mark in the radiotherapy process; and then carrying out weighted fusion on historical radiotherapy data, a gold label vector and a position relationship to obtain an accurate rotation compensation component, inputting the current position of the gold label into the model to update parameters in real time, predicting the position of the gold label at the next moment by combining the two types of compensation components, and meanwhile, carrying out model loop optimization through comparison of actual positions. According to the invention, the positioning accuracy of the target region in tumor radiotherapy is greatly improved, it is ensured that radioactive rays can accurately irradiate the tumor target region, the tumor local control rate is further improved, and the life quality of a patient is improved.

The invention belongs to the technical field of biological medicine, and particularly relates to a bionic photoactive nano preparation for tumor treatment as well as a preparation method and application thereof, and the preparation method comprises the following steps: synthesizing CaO2 (at) PDA-IR780 from nano calcium peroxide, polydopamine and iodide IR780, and synthesizing CaO2 (at) PDA-IR780 (at) M through a cell membrane coating technology. The obtained CaO2 (at) PDA-IR780 (at) M is a bionic photoactive nano preparation, the PDT effect of IR780 can be enhanced through in-situ oxygen production of nano calcium peroxide to improve the curative effect on tumors, controllable release of the preparation in the tumors is achieved through PDA acid response, and in-vivo long circulation and tumor targeted delivery are achieved through functional modification of nano particles through cell membranes. The photodynamic and photothermal activity is high, the tumor targeting characteristic and safety are good, and the application prospect in tumor treatment is wide.

Alkylphosphocholine analogs incorporating a chelating moiety that is chelated to gadolinium are disclosed herein. The alkylphophocholine analogs are compounds having the formula:or a salt thereof. R1 includes a chelating agent that is chelated to a gadolinium atom; a is 0 or 1; n is an integer from 12 to 30; m is 0 or 1; Y is —H, —OH, —COOH, —COOX, —OCOX, or —OX, wherein X is an alkyl or an arylalkyl; R2 is —N+H3, —N+H2Z, —N+HZ2, or —N+Z3, wherein each Z is independently an alkyl or an aroalkyl; and b is 1 or 2. The compounds can be used to detect solid tumors or to treat solid tumors. In detection / imaging applications, the gadolinium emits signals that are detectable using magnetic resonance imaging. In therapeutic treatment, the gadolinium emits tumor-targeting charged particles when exposed to epithermal neutrons.

The invention discloses an intelligent delivery system and method for tumor targeted therapy based on stem cell exosomes. The system comprises engineered exosomes secreted by engineered stem cells, and the engineered stem cells over-express cell-penetrating peptide-nuclear localization signal peptide fusion protein; a chemotherapeutic drug and a prodrug with tumor microenvironment responsiveness are entrapped in the engineered exosome. Genetic engineering modification is performed on stem cells, so that cell-penetrating peptide-nuclear localization signal peptide fusion protein is displayed on an exosome membrane secreted by the stem cells. The fusion protein not only endows the exosome with efficient transmembrane ability, but also can guide the exosome to be further enriched to the nucleus after entering the tumor cell by virtue of the nuclear localization signal (NLS) characteristic of the fusion protein, so that dual active targeting of tissue targeting (tumor) and organelle targeting (nucleus) is realized, and the concentration of a drug at an action site is greatly improved.

The invention relates to a tumor target volume segmentation method and device and a storage medium. The method comprises the following steps: acquiring a medical image containing a target tumor; segmenting the target tumor in a spatial dimension according to the medical image to obtain a plurality of first target volumes which are located in the target tumor and are not overlapped; segmenting the plurality of first target volumes in a time dimension to obtain a plurality of target volume sets corresponding to different fractions; wherein the target volumes in the target volume set corresponding to at least one adjacent fraction are not completely the same. Through the tumor target volume segmentation method and device and the storage medium provided by the invention, accurate segmentation of the tumor target volume can be realized, and the pertinence of radiotherapy is improved, so that the damage to biological tissues around an irradiation area where a tumor is located in the radiotherapy process is reduced.