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Folic acid and polydopamine modified tumor targeted mesoporous silica nanoparticle and preparation method and application thereof

A technology of mesoporous silica and polydopamine, which is used in anti-tumor drugs, medical preparations with inactive ingredients, and medical preparations containing active ingredients, etc. Toxicity, damage to normal body organs, etc., to achieve the effect of good tumor targeting and simple preparation method

Active Publication Date: 2017-06-09
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemotherapy and radiotherapy are the earliest developed and widely used treatment methods. Their main disadvantages are: the treatment methods are not highly targeted to cancer cells, and cannot effectively distinguish cancer cells from normal cells, especially chemotherapy and some protein drugs. Very high cytotoxicity, it also causes great damage to normal body organs during treatment
But this kind of nanomedicine does not have the function of active targeting

Method used

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  • Folic acid and polydopamine modified tumor targeted mesoporous silica nanoparticle and preparation method and application thereof
  • Folic acid and polydopamine modified tumor targeted mesoporous silica nanoparticle and preparation method and application thereof
  • Folic acid and polydopamine modified tumor targeted mesoporous silica nanoparticle and preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0043] A method for preparing folic acid and polydopamine-modified tumor targeting mesoporous silica nanoparticles, comprising the steps of:

[0044] (1) Weigh 100 mg of mesoporous silica MSNs and 10 mg of doxorubicin, dissolve them in 8 ml of deionized water, react in the dark for 6 hours under stirring conditions, centrifuge at 20,000 rpm for 15 min, discard the supernatant, and replace with deionized water Washing to remove free doxorubicin, the precipitate is vacuum-dried to obtain doxorubicin-loaded mesoporous silica primary nanoparticles MSNs-DOX;

[0045] (2) Resuspend the initial mesoporous silica nanoparticles MSNs-DOX in a 10 mM, pH=8.5 Tris buffer at a ratio of 5 mg:1 mL, and add dopamine hydrochloride to a concentration of 0.5 mg / mL , react in the dark for 3 hours, centrifuge at 10,000rpm for 30min, collect the precipitate, wash with deionized water to remove unreacted dopamine hydrochloride, and obtain doxorubicin-loaded mesoporous silica nanoparticles coated with...

Embodiment 2

[0059] A method for preparing folic acid and polydopamine-modified tumor targeting mesoporous silica nanoparticles, comprising the steps of:

[0060] (1) Weigh 100mg mesoporous silica MSNs and 2mg doxorubicin, dissolve in 8ml deionized water, react for 6 hours under stirring conditions, centrifuge at 20000rpm for 15min, discard the supernatant, wash with deionized water, and Remove free doxorubicin, and vacuum-dry the precipitate to obtain doxorubicin-loaded mesoporous silica primary nanoparticles MSNs-DOX;

[0061] (2) The initial mesoporous silica nanoparticles MSNs-DOX were resuspended in 10 mM PBS buffer solution with pH=8.5 according to the ratio of 1 mg:1 mL, and dopamine hydrochloride was added to make the concentration 0.2 mg / mL, React for 5 hours, centrifuge at 20,000rpm for 15min, collect the precipitate, wash with deionized water to remove unreacted dopamine hydrochloride, and vacuum-dry to obtain mesoporous silica nanoparticles MSNs wrapped by polydopamine loaded w...

Embodiment 3

[0065] A method for preparing folic acid and polydopamine-modified tumor targeting mesoporous silica nanoparticles, comprising the steps of:

[0066] (1) Weigh 100 mg of mesoporous silica MSNs and 20 mg of paclitaxel, dissolve in 10 ml of dimethyl sulfoxide, react for 12 hours under stirring conditions, centrifuge at 15,000 rpm for 15 min, discard the supernatant, and wash with deionized water , to remove free paclitaxel and solvent dimethyl sulfoxide, precipitate and vacuum-dry to obtain paclitaxel-loaded mesoporous silica primary nanoparticles MSNs-DTX;

[0067] (2) Resuspend the initial mesoporous silica nanoparticles MSNs-DTX in 10mM, pH=8.5 aqueous sodium hydroxide solution at a ratio of 10mg:1mL, and add dopamine hydrochloride to make the concentration 1.0mg / mL , react in the dark for 12 hours, centrifuge at 15000rpm for 20min, collect the precipitate, wash with deionized water to remove unreacted dopamine hydrochloride, and vacuum-dry to obtain paclitaxel-loaded polydop...

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Abstract

The invention provides a folic acid and polydopamine modified tumor targeted mesoporous silica nanoparticle and a preparation method and application thereof. The preparation method particularly comprises the following steps: (1) dissolving mesoporous silica and a chemical in a solvent, performing a full reaction, and performing separation; (2) adding mesoporous silica initial nano-particles obtained in the step (1) in a solution, adding dopamine hydrochloride, performing a full reaction, and performing separation; and (3) adding the dopamine hydrochloride coated mesoporous silica initial nano-particles loaded with the chemical in a weakly basic water solution, sequentially adding a reducing agent and polyethylene glycol modified sulfydryl grafted targeted ligand folic acid, performing a full reaction, then performing separation to obtain the folic acid and polydopamine modified tumor targeted mesoporous silica nanoparticle. The preparation method of the folic acid and polydopamine modified tumor targeted mesoporous silica nanoparticle is simple, and favorable tumor targeting ability, biocompatibility and biodegradability are achieved.

Description

technical field [0001] The invention relates to a tumor-targeting mesoporous silica nanoparticle modified by folic acid and polydopamine, a preparation method and application thereof. Background technique [0002] According to the report of the World Health Organization, cancer has become a major cause of threats to human life and health, and currently kills more than 7 million people around the world every year. At present, there are mainly the following methods for cancer treatment, such as: chemotherapy, radiotherapy, surgery, biological therapy, gene therapy, photodynamic therapy, cancer vaccines, etc. Chemotherapy and radiation therapy are the earliest developed and widely used treatment methods. Their main disadvantages are: the treatment methods are not highly targeted to cancer cells, and cannot effectively distinguish cancer cells from normal cells, especially chemotherapy and some protein drugs. High cytotoxicity, it also causes great damage to normal body organs ...

Claims

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Application Information

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IPC IPC(8): A61K9/14A61K47/34A61K47/22A61K47/04A61P35/00A61K31/704A61K31/337A61K31/55A61K31/192A61K31/513
CPCA61K9/146A61K31/192A61K31/337A61K31/513A61K31/55A61K31/704A61K47/02A61K47/22A61K47/34
Inventor 梅林曾小伟黄来强程伟梁超宇刘赣聂俊鹏
Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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