43 results about "Supporting cell" patented technology

The present invention discloses an air mattress system including a first protection cell group disposed on one side of a support cell group. The first protection cell group includes a first middle cell having a height not exceeding a support surface, so as to preserve smooth routing paths for medical treatment pipelines and prevent obstructed flow of fluids. A front and a rear of the first protection cell group are respectively provided with a first front cell and a first rear cell, both of which are provided with bottom air chambers. When a patient needs to be transferred, the bottom air chambers are controlled to be deflated so that tops of the first front cell and the first rear cell are substantially lowered to be near the support surface to provide the patient with an unobstructed transition during the transfer. When the height of the first protection cell group needs to be restored, only the bottom air chambers need to be inflated and a bed height suitable for a patient to lay on can be quickly restored. The present invention attends to requirements of both smooth pipeline routings and ease of transfer for a patient, hence improving practicability and quality of care of the air mattress system.

The invention relates to the technical field of biology, in particular to application of rosa roxburghii tratt active extract in preparation of a medicine for treating dyszoospermia. The extract provided by the invention is prepared from roxburgh rose fruits or roxburgh rose pomace through extraction and enrichment, the raw material resources are rich, the price is low, and the toxic and side effects are low. Animal experiments show that the roxburgh rose active extract has the activity of improving dyszoospermia, and the roxburgh rose active extract can obviously increase the sperm concentration and motility rate of mice with dyszoospermia, reduce the malformation rate and obviously increase the thickness of spermatogenic epithelium, the number of supporting cells and the number of interstitial cells of testicular tissues of the mice with dyszoospermia. WB detection analysis shows that the action way of improving the dyszoospermia by the roxburgh rose active extract is to inhibit activation of an oxidative stress Keap1 / Nrf2 signal channel. The rosa roxburghii tratt active extract has a new function of improving dyszoospermia and can be applied as a medicine for improving dyszoospermia.

Methods of transplanting target cells into a subject in need thereof. The methods generally comprise creating a sealed transplantation space by attaching an acellular membrane to the surface of a visceral organ or tissue in the subject in a manner such that a sealed space is created between the surface of the visceral organ or tissue and the membrane, and inserting a mixture of support cells and the target cells into the sealed transplantation space.

The present disclosure provides SL26A4 promoters and SLC26A4 enhancers, as well as vectors containing the same, that can be used to limit gene expression to cells expressing SLC26A4 and to increase gene expression in these cells, such as interdental cells, root cells, helicoid cells, and vestibular supporting cells. The SLC26A4 enhancers and SLC26A4 promoters described herein can be operably linked to polynucleotides encoding expression products, such as transgenes, and are used to treat subjects suffering from or at risk of developing hearing loss or vestibular dysfunction.

The invention relates to the field of biological medicines, and discloses a fused adeno-associated virus and application thereof in ear diseases and ophthalmic diseases. The fusion adeno-associated virus comprises a fusion peptide fragment formed by fusing peptide fragments of serotype AAV1, 2, 6 and 7 or a variant of the fusion peptide fragment, can efficiently transduce hair cells and / or supporting cells in a cochlea, promote regeneration of internal hair cells and help to recover hearing, can also efficiently infect an RPE layer, and is used for treating ophthalmic diseases. As a safe vector, the fused adeno-associated virus has a wide application prospect in treatment of ear diseases or ophthalmic diseases.

Hydrogels comprising a macromolecular matrix and water may be used to augment soft tissue of a human being, promote or support cell or tissue viability or proliferation, create space in tissue, and for other purposes. A macromolecular matrix may comprise a hyaluronic acid component crosslinked to a silk fibroin component.

The invention relates to the field of medical instruments, in particular to a needle knife and culture dish applied to extraction of wounded part tissue in the treatment process, the needle knife and culture dish comprises a charging wire, the bottom of the charging wire is connected with a fixing barrel, the fixing barrel is of a hollow barrel-shaped structure with an opening in the bottom end, and an inner cavity of the fixing barrel is slidably connected with a protection barrel; one end of the protective cylinder penetrates through an opening in the bottom end of the fixed cylinder and extends to the position below the fixed cylinder, the protective cylinder is of a hollow cylindrical structure with an opening in the bottom end, a needle knife is arranged in an inner cavity of the protective cylinder, a culture dish assembly is arranged at the end, away from the charging wire, of the protective cylinder, and the culture dish assembly is used for storing tissue samples. The knife handle can be pushed downwards or pulled upwards through the electric push rod, so that the needle knife fixed at the bottom end of the knife handle can probe downwards or contract upwards, the height of the needle knife is adjusted, the needle knife is protected, and meanwhile, the needle knife is prevented from injuring a patient accidentally.

Featured are methods, compositions, and apparatuses for the in vitro maturation of oocytes. In particular, the disclosure features methods of inducing oocyte maturation in vitro, by co-culturing a female subject's oocytes with an ex vivo composition containing a plurality of ovarian support cells (e.g., granulosa cells). Additional methods for administering follicular triggering agents and retrieving oocytes from the female subject are provided. Such methods, compositions, and apparatuses are particularly useful for assisted reproduction technology (ART) procedures.

Featured are methods, compositions, and apparatuses for the in vitro maturation of oocytes. In particular, the disclosure features methods of inducing oocyte maturation in vitro, by co-culturing a female subject's oocytes with an ex vivo composition containing a plurality of ovarian support cells (e.g., granulosa cells). Additional methods for administering follicular triggering agents and retrieving oocytes from the female subject are provided. Such methods, compositions, and apparatuses are particularly useful for assisted reproduction technology (ART) procedures.

Provided is a device which includes: an acquisition unit which acquires cell information that relates to cells cultured in first culture and culture information that indicates the culture conditions for the first culture; and an estimation unit which estimates the state of a culture medium after the first culture has been performed, on the basis of the cell information and the culture information. The acquisition unit also acquires post-treatment information that indicates the conditions for a post-treatment performed on the culture medium after the first culture has been performed. The estimation unit estimates the state of the culture medium after the first culture and the post-treatment have been performed, on the basis of the cell information, the culture information, and the post-treatment information.

Prosthetic heart valves including a radially expandable annular frame and valvular structure disposed within the frame, as well as methods and delivery apparatus associated therewith, are disclosed. The frame can include commissure-supporting cells for attachment of commissures of the valvular structure to the frame. The commissure-supporting cells can be offset from an outflow end of the frame by an offset distance and aligned with gaps in the frame. In some examples, the gaps are defined by a row of discontinuous cells in an outflow portion of the frame. In some examples, the gaps are defined by pairs of cells in an outflow portion of the frame. In some examples, the pairs of cells are enlarged relative to other cell of the frame.

This invention provides a method to improve age-related decline in ovarian function by supplementing quiescent oocytes within primordial follicles. [Solution] The present invention provides a method for inducing mature oocytes, comprising the steps of: preparing a single-cell suspension from the ovary of a mammal other than a human; separating the single-cell suspension into oocytes and ovarian somatic cells (supporting cells) within a primordial follicle using a flow cytometer; re-aggregating the oocytes and ovarian somatic cells within the separated primordial follicle; and maturing the re-aggregated primordial follicle in vitro.

Provided is an expression cassette for expressing GJB2 protein, which comprises a cochlear supporting cell and cochlear lateral wall-specific promoter and a nucleic acid encoding GJB2 protein. Also provided is a recombinant adeno-associated viral vector, the genome of which comprises an expression cassette for expressing GJB2 protein inserted between two ITR sequences; and a combination of at least two recombinant adeno-associated viral vectors. The recombinant adeno-associated viral vector or combination thereof can be used for treating or preventing hearing loss associated with defects in the GJB2 gene.

The present disclosure relates to compositions and methods for the conversion of source cells (such as cochlear support cells) to cochlea hair cells and / or cochlea hair cell-like cells by introducing transcription factors (TFs) into the source cells, and methods for treating hearing loss.

The invention provides a separation method and an identification method of bactrian camel testis interstitial cells, and belongs to the technical field of cell separation. The method comprises the following steps: mixing 0.08-0.12% of collagenase IV solution and 0.23-0.27% of trypsin-EDTA (Ethylene Diamine Tetraacetic Acid) according to a volume ratio of 1: 2, digesting a bactrian camel testis tissue block, then carrying out hypotonic treatment purification to remove redundant germ cells, carrying out differential adhesion purification to remove a large amount of supporting cells, carrying out percoll density gradient centrifugation purification to remove a small amount of supporting cells, and carrying out freeze drying to obtain a finished product. And transferring the suspension with the percoll gradient of 34-60% into a new culture bottle to obtain the interstitial cells with the purity of more than or equal to 95%, and completing identification by combining morphological observation, immunofluorescence staining and secretion function detection. The separated bactrian camel testis interstitial cells can be used for double-hump reproductive performance research, and a foundation is laid for the reproductive performance of bactrian camels.

This disclosure features methods and compositions for the in vitro maturation of oocytes. In particular, this disclosure features methods and compositions relating to the manipulation of multiple ovarian supporting cells (OSCs) to promote the maturation of oocytes in a culture medium. Such methods and compositions are particularly useful in assisted reproductive technology (ART) procedures.

The invention relates to the technical field of micro-fluidic organ chips, and discloses a micro-fluidic testis organ chip and a culture method, the micro-fluidic testis organ chip comprises a PDMS fluid control layer and a PDMS cell culture layer from top to bottom; the at least three culture units are arranged on the PDMS cell culture layer in the axial direction, each culture unit internally comprises three functional areas, the three functional areas are used for testicular sertoli cell culture, spermatogenic cell culture and basement membrane simulation respectively, and the three functional areas are communicated with one another to form a fluid path; the regulation and control part is arranged on the PDMS fluid control layer, and the regulation and control part is used for regulating and controlling fluid paths which are communicated with one another among the three functional areas; the inoculation and collection part is arranged on the PDMS fluid control layer and is used for carrying out cell inoculation and collection in the three functional areas. The in-vivo testis spermatogenesis microenvironment is highly simulated, the limitation of a traditional 2D cell experiment and an animal experiment can be effectively made up, and a new method and platform are provided for analyzing a spermatogenesis disorder mechanism and screening targeted repair drugs.

The invention relates to the field of biological medicine, in particular to application of capsid protein to preparation of products for preventing or treating ear diseases or ear hair cell regeneration treatment products. The capsid protein disclosed by the invention comprises a peptide fragment of which the amino acid sequence is shown as any one of SEQ ID No.1-3. The adeno-associated virus containing the capsid protein can mediate a target gene to be specifically expressed in cochlear hair cells of an adult mouse under the condition of posterior semicircular canal injection of the adult mouse; under the condition of round window injection of the young mouse, the target gene is mediated to be specifically expressed in cochlea supporting cells of the young mouse, so that the adeno-associated virus disclosed by the invention can provide a safe, efficient and specific transduction tool for otologic gene therapy.

The disclosure provides SL26A4 enhancers and SLC26A4 promoters, as well as vectors containing the same, that can increase gene expression in SLC26A4-expressing cells, such as interdental cells, root cells, spiral prominence cells, and vestibular supporting cells. The SLC26A4 enhancers and SLC26A4 promoters described herein may be operably linked to a polynucleotide, such as a transgene, encoding an expression product and used for the treatment of subjects having or at risk of developing hearing loss or vestibular dysfunction.

The present disclosure provides polynucleotides containing a TMPRSS3 promoter of a region capable of specifically expressing a transgene in cochlear hair cells and sensory supporting cells. The disclosure also provides nucleic acid vectors containing these promoters operably linked to a polynucleotide encoding a polypeptide. The present disclosure provides compositions and methods for specifically inducing transgene expression in inner ear cells, such as hair cells and sensory supporting cells. The compositions described herein can be administered to a subject to treat a condition caused by inner ear cell dysfunction, such as hearing loss or vestibular dysfunction.

The present disclosure provides a bioengineered scaffold, a preparation method thereof, and regenerative tissue, relating to the fields of tissue engineering and regenerative medicine. The bioengineered scaffold provided by the present disclosure retains the complex and unobstructed vascular structure of the target organ. As an engineered material, it has a biomimetic structure and mechanical strength that closely resembles that of a living target organ, supports cell adhesion and growth, facilitates the formation of new functional tissues for medical purposes, and provides new ideas for the construction of in vitro organ function models.

The invention discloses a Gjb2 gene enhancer and application thereof, and belongs to the field of gene engineering, the enhancer can significantly enhance the expression of a Gjb2 gene or other genes (such as a GFP gene) in an inner ear supporting cell, and can be used for gene therapy of deafness by utilizing the ability of the enhancer to specifically enhance the gene expression in the inner ear supporting cell; the Gjb2 gene enhancer is integrated and prepared into recombinant DNA containing the Gjb2 gene enhancer, a carrier or an adenovirus and other biological materials, gene expression regulation and control research is carried out, and the application prospect is good.

The disclosure provides polynucleotides containing SLC6A14 promoters, as well as vectors containing the same, that can be used to promote expression of a transgene in vestibular supporting cells. The polynucleotides described herein may be operably linked to a transgene, such as a transgene encoding a protein of interest, so as to promote vestibular supporting cell expression of the transgene. The polynucleotides described herein may be operably linked to a transgene and used for the treatment of subjects having or at risk of developing vestibular dysfunction.

The invention discloses application of sodium 4-phenylbutyrate in a product for improving dyszoospermia of obese males, and belongs to the technical field of biological medicines. High-fat feed is adopted to construct a rat obesity model, the protection effect of 4-PBA on the reproductive function of obese rats is studied, and experimental results show that after the 4-PBA intervenes the obese rats, the testosterone content can be obviously increased, the estradiol level can be reduced, the sperm motility can be improved, the sperm malformation rate can be reduced, the spermatogenic dysfunction can be obviously relieved, and the anti-obesity effect on testis tissue morphology, testis tissue morphology, testis tissue morphology, testis tissue morphology and testis tissue morphology can be improved. The functions of supporting cell mitochondria, endoplasmic reticulum and MAM structures are obviously improved, and a new treatment strategy and a new drug target are provided for intervening and controlling obesity induced testis dyszoospermia and improving fertility of obese males.

The invention relates to the field of fertility preservation and protection before adolescence, in particular to a transition culture method before immature testicular tissue transplantation. In a transition culture process before immature testicular tissue transplantation, coexistence states (such as FSH and testosterone continuously exist in a male body) of various growth factors and hormones in an in-vivo microenvironment are simulated, or in-vivo physiological dynamic changes are simulated. According to the transition culture method disclosed by the invention, the huge environmental difference caused by transplanting the testicular tissue before adolescence to the mature testicular tissue is reduced, the proliferation of spermatogonium and the maturation of supporting cells are remarkably promoted, the survival rate of the spermatogonium after transplantation is improved, and a foundation is laid for spermatogenesis after transplantation.

Provided herein are methods of co-culturing a myogenic cell with a support cell to increase myotube formation from the myogenic cells. For example, provided herein is a method comprising co-culturing a myogenic cell with a support cell wherein the support cell comprises a polynucleotide comprising a coding sequence of a gene of interest; and culturing the myogenic cell and the support cell in a cultivation infrastructure under conditions sufficient to induce proliferation of the myoblast cell.

The invention is applicable to the technical field of biological medicine, and provides a resveratrol carbon dot-loaded cell membrane bionic nano-vesicle and application thereof, and the resveratrol carbon dot-loaded cell membrane bionic nano-vesicle is prepared by the following preparation process: mixing pure trans-resveratrol with ultrapure water, adjusting the pH value until complete dissolution, reacting the solution to obtain a carbon dot solution, centrifuging, filtering with a filter membrane, dialyzing and purifying to obtain the resveratrol carbon dot-loaded cell membrane bionic nano-vesicle. Carrying out rotary evaporation and freeze-drying to obtain RES CDs; and dropwise adding Cy7 into the DSPE-PEG solution, stirring overnight, dialyzing to remove free Cy7 to obtain Cy7-coated PEG, and mixing TM4-M, RES CDs and Cy7-coated PEG to obtain the resveratrol carbon dot-loaded cell membrane bionic nano-vesicles RCD-coated PCy-coated EVs. The invention also discloses a preparation method of the resveratrol carbon dot-loaded cell membrane bionic nano-vesicles RCD-coated PCy-coated EVs and application of the resveratrol carbon dot-loaded cell membrane bionic nano-vesicles. The vesicles based on the supporting cell membrane prepared by the invention can specifically deliver the resveratrol carbon dots to the blood testis barrier and treat spermatogenesis disorder.

The present disclosure provides polynucleotides and nucleic acid vectors containing a GJB2 regulatory construct operably linked to a polynucleotide encoding a gap junction protein beta2 (Gjb2) protein (e.g., a wild-type human Gjb2 protein). These polynucleotides and vectors can be used to express Gjb2 in cells expressing GJB2, including cochlear supporting cells, and thus can be used to treat subjects suffering from or at risk of developing GJB2-related hearing loss.

The application belongs to the technical field of gene therapy, and particularly relates to an AAV capsid protein mutant targeting glial cells and application thereof. The AAV capsid protein mutant is constructed by inserting a polypeptide with glial cell infection characteristics between N589 and R590 of an AAV-DJ capsid protein amino acid sequence, and exhibits enhanced transduction and targeting in inner ear basal membrane supporting cells, and has the function of enhancing supporting cell transduction in the cochlear basal membrane of newborn and adult mice, thereby being capable of solving the problem of low transduction efficiency of AAV in the cochlear basal membrane supporting cells of adult mice, and providing a potentially available tool for gene therapy of deafness genes positioned in supporting cells by Chen et al. GJB2 and the like.