294 results about "Supporting cell" patented technology

Cells derived from postpartum tissue having the potential to support cells of and / or differentiate to cells of a soft tissue lineage, and methods of preparation and use of those postpartum tissue-derived cells, are provided by the invention. The invention also provides methods for the use of such postpartum-derived cells and products related thereto in therapies for conditions of soft tissue.

The present invention provides a variety of microscale bioreactors (microfermentors) and microscale bioreactor arrays for use in culturing cells. The microfermentors include a vessel for culturing cells and means for providing oxygen to the interior of the vessel at a concentration sufficient to support cell growth, e.g., growth of bacterial cells. Depending on the embodiment, the microfermentor vessel may have various interior volumes less than approximately 1 ml. The microfermentors may include an aeration membrane and optionally a variety of sensing devices. The invention further provides a chamber to contain the microfermentors and microfermentor arrays and to provide environmental control. Certain of the microfermentors include a second chamber that may be used, e.g., to provide oxygen, nutrients, pH control, etc., to the culture vessel and / or to remove metabolites, etc. Various methods of using the microfermentors, e.g., to select optimum cell strains or bioprocess parameters are provided. The invention provides microreactors having a variety of different designs, some of which incorporate active stirring and / or have the capability to operate in batch or fed-batch mode. The invention further provides an apparatus and methods for simultaneous operation of a plurality of microreactors, with monitoring of the individual microreactors during a run. The invention further provides methods of performing gene expression analysis on cells cultured in microreactors.

In one embodiment, the digital bed system is comprised of an array of support cells. Each support cells is capable of communicating with a controller and increasing and decreasing in firmness in response to commands issued by a controller. The support cells are operatively connected to a communication channel that is also connected to a controller. The controller is capable of receiving data from the support cells and is also capable of issuing commands to each of the support cells. The controller is programmed issue commands to increase or decrease the firmness of individual support cells within the support cells.

Stem cells products having the potential to support cells of a soft tissue lineage, and methods of preparation and use of those stem cell products are disclosed. The invention also relates to methods for the use of such stem cells products in the regeneration and repair of soft tissue, and in cell-based therapies for of soft tissue conditions.

A network for a cellular communication system comprises access points (105-109) supporting cells within a region (113). Each access point (105-109) has an individual proxy address of a proxy address space which is a local address space of an address proxy (101) and a common network address of a network address space which is a network wide address space. A gateway access point (103) covers an entry point to the region (113) and detects a remote station entering the region. It then determines an access point (105) in the region to which the remote station is handed over and transmits a binding message to the address proxy (101) with an indication of the access point (105). In response to receiving the binding message, the address proxy (101) establishes a binding between the common network address and the proxy address of the access point (105). Data for the remote station is then forwarded to the access point (105) using the binding.

A kit of parts is provided for assembly into exercise apparatus of various configurations, as desired and defined by the user. The apparatus, when configured to form a supporting cell, conveniently allows the attachment of a plurality of exercise devices of the same or different type while, in addition, can be used as a storage center for items normally found at a fitness center such as bands, weights, balls, bars, barbells, etc.

An ultrasound imaging system with a digital scanning architecture for configuring connections that transmit pulser timing signals among transducer subelements. The system provides multiple operating states that each generate a different wave pattern. An integrated circuit structure includes a plurality of circuit support cells, each including pulser circuitry for operation of at least one of the subelements. The circuit structure also includes first connections each selectable through one of multiple first access switches, these providing a timing signal to the pulser circuitry in one of the circuit support cells. Some of the support circuit cells further include one or more timing channel select switches providing selectable connections to transmit different pulser timing signals among the first access switches.

A novel cell seeded hollow fiber bioreactor is described as a potential bioartificial kidney. Endothelial cells along with pericyte, vascular smooth muscle, and / or mesangial cells or any mesenchymally derived support cells are seeded along a hollow fiber in a perfused bioreactor to reproduce the ultrafiltration function and transport function of the kidney. Maintenance of tissue specific function and ultrastructure suggest that this bioreactor provides an economical device for treating renal failure.

A co-culture apparatus and method for, among other things, converting a progenitor cell line into a target cell line. The apparatus comprises an exterior receptacle, a removable cartridge that fits into the exterior receptacle, and a closure member to seal the apparatus. The invention is also directed to a method for transforming a progenitor cell line into a target cell line. In one embodiment of the invention, the method comprises the steps of: establishing a progenitor cell line; establishing a support cell line; co-culturing said progenitor and support cell lines; establishing a transformation cell line; and co-culturing said progenitor and transformation cell lines, wherein the transformation cell line supports the transformation of the progenitor cell line into a target cell line.

A three-dimensional (3-D) culture “Petri-dish” for research in regenerative medicine, biotechnology and clinical translation is described. This 3-D perfusion culture dish is to advance in vitro culture tools from static 2-D to dynamic 3-D perfusion culture. Interwoven hollow fiber capillary membranes divide the “Petri-dish” culture space into a controllable 3-D pattern of different compartments, serving the functions of the organ's larger vasculature. These physically active scaffolds, which can be suitable for cell adhesion or cell aggregate immobilization, offer a supply of cells with high-performance mass exchange including gas supply and under perfusion conditions. In contrast to static and discontinuous medium supply, a dynamic culture can be achieved with continuous or alternating medium supply and integral oxygenation. They provide a more physiologic supply in the cell macro environment, including homeostasis of oxygen, pH, nutrition, soluble factors, and gradients of metabolites for the cells. Also, medium perfusion can be achieved. Consequently the invention was made for cultures at tissue density, especially stem cells and support cells, which strive to create their own stem cell niche.

A matrix for tissue growth includes: (a) a first layer including a first assembly of collagen fibers; (b) a plurality of projections on a top surface of the first layer; and (c) a second layer bonded to a bottom portion of the first layer and including a second assembly of collagen fibers, wherein the second layer has a lower density than the first layer, and the matrix includes pores effective to support cell growth into the matrix. A method for providing the matrix is also described.

The present invention relates to targeted delivery systems for delivering therapeutic agents to tumor. The invention further relates to methods of delivering a therapeutic agent to a tumor for the prevention and treatment of cancer by killing tumor cells and tumor-associated endothelial cells. In particular, the present invention provides a tumor-targeted drug delivery system comprising a NGR-containing molecule linked to a delivery vehicle encapsulating a therapeutic agent, preferably a drug, such as a cytotoxic agent or a chemotherapeutic agent. Specifically, the delivery systems of the present invention are capable of delivering an increased amount of therapeutic agent to a tumor as compared to other delivery systems. In particular, the delivery systems of the present invention are capable of accumulating a higher amount of therapeutic agent in a tumor, or in the vicinity of a tumor cell or tumor-supporting cell, resulting in exposure of the tumor cell and tumor-associated endothelial cell to therapeutic levels of the agent for a longer period of time as compared to other delivery systems. The present invention also describes pharmaceutical compositions comprising the delivery systems of the present invention. The present invention further relates to a tumor treatment comprising an increased amount of therapeutic agent delivered by the system of the present invention as compared to other delivery systems. The delivery systems and pharmaceutical compositions can be administered to a subject, preferably a human, alone or in combination, sequentially or simultaneously, with other prophylactic or therapeutic agents and / or anti-cancer treatments.

The present invention provides a variety of microscale bioreactors (microfermentors) and microscale bioreactor arrays for use in culturing cells. The microfermentors include a vessel for culturing cells and means for providing oxygen to the interior of the vessel at a concentration sufficient to support cell growth, e.g., growth of bacterial cells. Depending on the embodiment, the microfermentor vessel may have various interior volumes less than approximately 1 ml. The microfermentors may include an aeration membrane and optionally a variety of sensing devices. The invention further provides a chamber to contain the microfermentors and microfermentor arrays and to provide environmental control. Certain of the microfermentors include a second chamber that may be used, e.g., to provide oxygen, nutrients, pH control, etc., to the culture vessel and / or to remove metabolites, etc. Various methods of using the microfermentors, e.g., to select optimum cell strains or bioprocess parameters are provided. The invention provides microreactors having a variety of different designs, some of which incorporate active stirring and / or have the capability to operate in batch or fed-batch mode. The invention further provides an apparatus and methods for simultaneous operation of a plurality of microreactors, with monitoring of the individual microreactors during a run. The invention further provides methods of performing gene expression analysis on cells cultured in microreactors.

A solid oxide fuel cell (SOFC) includes a cathode electrode, an anode electrode, and a solid oxide electrolyte located between the anode electrode and the cathode electrode. The cathode electrode is a porous ceramic layer infiltrated with a cathode catalyst material, and the anode electrode is a porous ceramic layer infiltrated with an anode catalyst material, and the electrolyte is a ceramic layer having a lower porosity than the anode and the cathode electrodes. A ceramic reinforcing region may be located adjacent to the riser opening in the electrolyte.

A method of cell expansion is provided. The method comprising culturing adherent cells from placenta or adipose tissue under three-dimensional culturing conditions, which support cell expansion.

The present invention provides a communication method, a user device, a base station and a communication device. The method comprises: the base station sends the random access response information to the user device UE,and the random access response information comprises a time-advance order which has a length of 12bit or has a dereference of 1282. The present invention can make the base station send the time-advance order with a length of at least 12bit or a dereference of greater than 1282 in the random access response information so as to make the UE acquire greater time calibration quantity during the process for time calibration, thus being able to support cell coverage of longer distance.

This invention discloses a dyeing kettle of the over-critical carbon-dioxide dyeing device, which is to use for the dyeing of the textile, including arranging the barrel and the sealed convex shoulder flat that is on the upper part of the barrel; the center opening of the barrel's bottom connects with the first connector, the inside center opening of barrel connects with the inner barrel by the support cell; the wall of the inner barrel is arranged with the filter hole uniformly, the upper of the inner barrel connects with the second cover; the barrel wall opening of the barrel's upper connects with the second connector; the under part of the barrel connects with the nip cover, which has the ring space with the barrel, and the upper of the ring space is sealed in the protruding stage that outside the barrel, and the first cover is sealed in the under part, one side of the ring space's under part opening connects with the first adapter, and the other side of upper part opening connects with the second adapter. This invention realizes the combining of the technology of the textile internal dyeing and external dyeing, and the dynamic dyeing in the over-critical dyeing method.

It relates to a solid oxide fuel cell (SOFC) with internal reforming of hydrocarbons, in which said cell is a metal-supported cell comprising a porous metallic support comprising pores having walls, said porous support comprising a first main surface and a second main surface, an anode adjacent to said second main surface, an electrolyte adjacent to said anode, and a cathode adjacent to said electrolyte, a catalyst for reforming at least one hydrocarbon being deposited on the walls of the pores of the porous metallic support, and the amount and concentration of catalyst in the porous metallic support decreasing in a direction from the first main surface in the same direction as a flow direction of a hydrocarbon feed stream, along said first main surface on the outside of the cell.

A solid oxide fuel cell (SOFC) has a porous electrode support structure on both sides of a thin electrolyte layer. The porous electrode supported cell is formed with gas flow plenum channels on an outer surface of the electrode scaffold.

A network for a cellular communication system comprises access points (105-109) supporting cells within a region (113). Each access point (105-109) has an individual proxy address of a proxy address space which is a local address space of an address proxy (101) and a common network address of a network address space which is a network wide address space. A gateway access point (103) covers an entry point to the region (113) and detects a remote station entering the region. It then determines an access point (105) in the region to which the remote station is handed over and transmits a binding message to the address proxy (101) with an indication of the access point (105). In response to receiving the binding message, the address proxy (101) establishes a binding between the common network address and the proxy address of the access point (105). Data for the remote station is then forwarded to the access point (105) using the binding.

An ultrasound system and method of configuring an ultrasound system. In one embodiment, the system includes an array of transducer cells grouped in acoustical subelements. Transducer cells in different acoustical subelements are operable according to different pulser timing signals. An integrated circuit structure includes an array of circuit support cells. A first of the support cells provides circuitry for implementing selectable timing signals and high voltage pulse generation to propagate acoustic signals from multiple transducer cells. Connective paths extend between the transducer cells and the first of the circuit support cells to effect generation of acoustic signals and receipt of echo data. A first of the paths extends between a first transducer cell positioned in a first of the different ones of the acoustical subelements and circuitry in the first of the support cells, and a second of the paths extends between a second transducer cell positioned in a second of the different ones of the acoustical subelements and circuitry in the first of the support cells.

A novel cell seeded hollow fiber bioreactor is described as a potential bioartificial kidney. Endothelial cells along with pericyte, vascular smooth muscle, and / or mesangial cells or any mesenchymally derived support cells are seeded along a hollow fiber in a perfused bioreactor to reproduce the ultrafiltration function and transport function of the kidney. Maintenance of tissue specific function and ultrastructure suggest that this bioreactor provides an economical device for treating renal failure.

A pipette washer comprising a plurality of cells for receiving pipettes is disclosed. Each of the cells comprises a top opening for receiving pipettes to be washed and a bottom opening. A support member supports the cells with the top opening accessible to receive pipettes to be washed. A hose system is coupled to the bottom opening on the cells. A supply hose is connected to the hose system. Each of the cells is supported in the support member with an orientation extending transverse to the horizontal. Each of the support cells comprises a cell member having a top width and a bottom width. The width is wider at the top and narrower at the bottom. In accordance with the preferred embodiment, each cell member is conical in shape. A housing contains and rechannels washing liquid while at the same time having an open front to allow for convenient access to the cells.

There is provided scaffolds for tissue repair / augmentation / implant comprising an acellular matrix, a biocompatible polymer and a biomimetic agent. The scaffolds advantageously supports cell growth in the target tissue. There is also provided a method for the preparation of the scaffold and for monitoring the functionality of the scaffold in tissue using dynamic contrast-enhanced magnetic resonance imaging.

The invention discloses a cathode-supported direct carbon fuel cell, which comprises a fuel bin, an anode side collector layer, an anode layer, an electrolyte layer, a cathode active layer, a cathode-supporting layer, a cathode side collector layer, a carbon fuel layer, a porous ceramic layer, a carbon fuel input tube, a carrier gas intake tube, a carrier gas output tube and an oxygen or air intake tube, a cell is arranged in the fuel bin, the anode side collector layer, the anode layer, the electrolyte layer, the cathode active layer, the cathode-supporting layer and the cathode side collector layer are arranged sequentially from the outside to the inside, and moreover, the anode side collector layer, the carbon fuel layer and the porous ceramic layer are superposed in the fuel bin sequentially from the top down. Since the cell is a cathode-supported cell which directly utilizes solid carbon as fuel and the outside of the cell is provided with the fuel input tube, the cell has the advantages of simple structure, high energy efficiency, environment-friendliness, no leakage, corrosion and explosion dangers and the like, can realize the continuous feeding of fuel and the diversity of the fuel electrode, and is convenient and easy to operate.

A two-mode therapeutic mattress system is provided, including a non-powered air mattress, a pressure dispersion cushion, and a selectively operable air diffusion coverlet in a common modular assembly for improved medical management of skin care. The non-powered air mattress includes longitudinal air cylinders providing a static air support system inflated to predetermined pressure. The longitudinal air cylinders are in pressure communication with elasticized reservoirs, in that the air level in the elasticized reservoirs dynamically reacts to changes in pressure in the longitudinal air cylinders, for example upon receipt of a patient upon the mattress system. The pressure dispersion cushion facilitates pressure relief with a number of lateral and longitudinal cuts resulting in a plurality of separate upright support cells, the size and construction of which may vary over the surface of the pressure dispersion cushion so as to provide selective support characteristics. The air diffusion coverlet contains a plurality of air pockets. The air diffusion coverlet operates in two modes: a “therapy mode” such that compressed air from an air compressor is pumped to the air diffusion coverlet, and an “off” mode in which the air pockets collapse to provide a suitable and relatively smooth bedding component to the therapeutic mattress system. The non-powered air mattress, pressure dispersion cushion, and selectively operable air diffusion coverlet cooperate to provide a single product that addresses localized pressure, maceration, shear stress, and dynamic changes in a patient's condition during the course of time.