86 results about "Supporting cell" patented technology

The present invention discloses an air mattress system including a first protection cell group disposed on one side of a support cell group. The first protection cell group includes a first middle cell having a height not exceeding a support surface, so as to preserve smooth routing paths for medical treatment pipelines and prevent obstructed flow of fluids. A front and a rear of the first protection cell group are respectively provided with a first front cell and a first rear cell, both of which are provided with bottom air chambers. When a patient needs to be transferred, the bottom air chambers are controlled to be deflated so that tops of the first front cell and the first rear cell are substantially lowered to be near the support surface to provide the patient with an unobstructed transition during the transfer. When the height of the first protection cell group needs to be restored, only the bottom air chambers need to be inflated and a bed height suitable for a patient to lay on can be quickly restored. The present invention attends to requirements of both smooth pipeline routings and ease of transfer for a patient, hence improving practicability and quality of care of the air mattress system.

The present invention relates to the field of biomedicine, and discloses a fusion-type adeno-associated virus and its application in ear diseases and ophthalmic diseases. The fusion-type adeno-associated virus comprises a fusion peptide segment or a variant thereof formed by fusing peptide segments of serotypes AAV1, 2, 6 and 7, which can efficiently transduce hair cells and / or supporting cells in the cochlea, promote the regeneration of inner hair cells, and help restore hearing. The adeno-associated virus can also efficiently infect the RPE layer and is used for the treatment of ophthalmic diseases. As a safe vector, the fusion-type adeno-associated virus has broad application prospects in the treatment of ear diseases or ophthalmic diseases.

The invention discloses a method for constructing an organoid. The method comprises the following steps: (1) carrying out hanging drop culture on testicular supporting cells and primordial germ cell-like cells in an organoid culture solution according to a quantity ratio of 1: (2-6) to obtain cell aggregates; (2) culturing the cell aggregate in a gas-liquid phase culture system to obtain an organoid; wherein the content of double positive cells in the primordial germ cell-like cells is greater than 40%, the gas-liquid phase culture system comprises a solid culture medium and an organoid culture solution, and the solid culture medium is formed by the organoid culture solution and agarose gel; wherein the organoid culture solution is prepared from human bone morphogenetic protein-4, a basic fibroblast growth factor, a glial cell-derived neurotrophic factor and retinoic acid. The organoid obtained by the method has a high germ cell population proportion.

The invention relates to the technical field of biology, in particular to application of rosa roxburghii tratt active extract in preparation of a medicine for treating dyszoospermia. The extract provided by the invention is prepared from roxburgh rose fruits or roxburgh rose pomace through extraction and enrichment, the raw material resources are rich, the price is low, and the toxic and side effects are low. Animal experiments show that the roxburgh rose active extract has the activity of improving dyszoospermia, and the roxburgh rose active extract can obviously increase the sperm concentration and motility rate of mice with dyszoospermia, reduce the malformation rate and obviously increase the thickness of spermatogenic epithelium, the number of supporting cells and the number of interstitial cells of testicular tissues of the mice with dyszoospermia. WB detection analysis shows that the action way of improving the dyszoospermia by the roxburgh rose active extract is to inhibit activation of an oxidative stress Keap1 / Nrf2 signal channel. The rosa roxburghii tratt active extract has a new function of improving dyszoospermia and can be applied as a medicine for improving dyszoospermia.

The present disclosure provides SL26A4 enhancers and SLC26A4 promoters, as well as vectors containing the same, that can increase gene expression in cells expressing SLC26A4, such as interdental cells, root cells, helicoid cells, and vestibular supporting cells. The SLC26A4 enhancers and SLC26A4 promoters described herein can be operably linked to polynucleotides encoding expression products, such as transgenes, and are used to treat subjects suffering from or at risk of developing hearing loss or vestibular dysfunction.

The invention discloses application of a NOTCH pathway ligand JAG1 in regulation and control of cochlea hair cell regeneration, and relates to the technical field of cochlea hair cell regeneration. According to the invention, JAG1 in cochlea supporting cells plays a regulating role through a NOTCH1 / 2 receptor, and NOTCH3 does not play a role; in addition, PI3K-Akt-mTOR signal channels in cochlea supporting cells can be reduced due to deficiency of the P2 mouse JAG1, so that the proliferation level of the supporting cells is reduced, and the supporting cells are further dedifferentiated into hair cells; meanwhile, it is proved that the high-level JAG1 / Notch can promote the proliferation level of cochlea supporting cells; the invention also discloses that the expression of the JAG1 is reduced along with the maturation of the supporting cells after the mouse is born, which indicates that the JAG1 is associated with the plasticity of the supporting cells. Most importantly, the research finds that overexpression of JAG1 / ATOH1 in P2 mouse cochlea supporting cells can promote hair cell formation.

The present application relates to the field of biomedicine, and specifically to a fusion adeno-associated virus capsid protein, adeno-associated virus and its application. The present application provides a fusion adeno-associated virus AAV-WM14 capsid protein, comprising amino acid fragments of serotypes AAV1, AAV2, AAV3, AAV4, AAV6, AAV7, AAV8, AAV10, and AAV13, and the capsid protein includes the amino acid sequence shown in SEQ ID NO.1. The fusion adeno-associated virus provided in the present application can be administered by intravitreal injection, efficiently infecting retinal photoreceptor cells in the eye, and can be delivered to the cochlea to efficiently infect inner hair cells and supporting cells. The fusion adeno-associated virus provided in the present application can be widely used in gene therapy for related diseases.

Methods of transplanting target cells into a subject in need thereof. The methods generally comprise creating a sealed transplantation space by attaching an acellular membrane to the surface of a visceral organ or tissue in the subject in a manner such that a sealed space is created between the surface of the visceral organ or tissue and the membrane, and inserting a mixture of support cells and the target cells into the sealed transplantation space.

The present disclosure provides SL26A4 promoters and SLC26A4 enhancers, as well as vectors containing the same, that can be used to limit gene expression to cells expressing SLC26A4 and to increase gene expression in these cells, such as interdental cells, root cells, helicoid cells, and vestibular supporting cells. The SLC26A4 enhancers and SLC26A4 promoters described herein can be operably linked to polynucleotides encoding expression products, such as transgenes, and are used to treat subjects suffering from or at risk of developing hearing loss or vestibular dysfunction.

The invention relates to the field of biological medicines, and discloses a fused adeno-associated virus and application thereof in ear diseases and ophthalmic diseases. The fusion adeno-associated virus comprises a fusion peptide fragment formed by fusing peptide fragments of serotype AAV1, 2, 6 and 7 or a variant of the fusion peptide fragment, can efficiently transduce hair cells and / or supporting cells in a cochlea, promote regeneration of internal hair cells and help to recover hearing, can also efficiently infect an RPE layer, and is used for treating ophthalmic diseases. As a safe vector, the fused adeno-associated virus has a wide application prospect in treatment of ear diseases or ophthalmic diseases.

Hydrogels comprising a macromolecular matrix and water may be used to augment soft tissue of a human being, promote or support cell or tissue viability or proliferation, create space in tissue, and for other purposes. A macromolecular matrix may comprise a hyaluronic acid component crosslinked to a silk fibroin component.

The invention relates to the field of medical instruments, in particular to a needle knife and culture dish applied to extraction of wounded part tissue in the treatment process, the needle knife and culture dish comprises a charging wire, the bottom of the charging wire is connected with a fixing barrel, the fixing barrel is of a hollow barrel-shaped structure with an opening in the bottom end, and an inner cavity of the fixing barrel is slidably connected with a protection barrel; one end of the protective cylinder penetrates through an opening in the bottom end of the fixed cylinder and extends to the position below the fixed cylinder, the protective cylinder is of a hollow cylindrical structure with an opening in the bottom end, a needle knife is arranged in an inner cavity of the protective cylinder, a culture dish assembly is arranged at the end, away from the charging wire, of the protective cylinder, and the culture dish assembly is used for storing tissue samples. The knife handle can be pushed downwards or pulled upwards through the electric push rod, so that the needle knife fixed at the bottom end of the knife handle can probe downwards or contract upwards, the height of the needle knife is adjusted, the needle knife is protected, and meanwhile, the needle knife is prevented from injuring a patient accidentally.

Featured are methods, compositions, and apparatuses for the in vitro maturation of oocytes. In particular, the disclosure features methods of inducing oocyte maturation in vitro, by co-culturing a female subject's oocytes with an ex vivo composition containing a plurality of ovarian support cells (e.g., granulosa cells). Additional methods for administering follicular triggering agents and retrieving oocytes from the female subject are provided. Such methods, compositions, and apparatuses are particularly useful for assisted reproduction technology (ART) procedures.

The invention discloses a differential expression gene of porcine sertoli cells under a heat stress condition and a screening method, and belongs to the technical field of biology. Comprising an up-regulated expression gene HSP90AA1 as shown in SEQ ID No. 1; hSPA6, which is as shown in SEQ ID No. 2; the HSPH1 is as shown in SEQ ID No. 3; the HSPB8 is as shown in SEQ ID No. 4; hSPE1, which is as shown in SEQ ID No.5; sOD2, as shown in SEQ ID No. 6; the TRPV1 is as shown in SEQ ID No. 7; the MAPK8IP2 is as shown in SEQ ID No. 8; pPARGC1A, which is as shown in SEQ ID No. 9; the down-regulated expression gene PPARGC1B is as shown in SEQ ID No. 10. The invention also discloses a preparation method of the gene. According to the invention, the inherent genetic genes of the porcine sertoli cells are taken as a starting point, the heat stress related genes of the porcine sertoli cells are analyzed based on the transcriptome, the heat stress related genotypes are screened out, and the cognition of heat stress response and adaptation of the porcine sertoli cells is improved.

Featured are methods, compositions, and apparatuses for the in vitro maturation of oocytes. In particular, the disclosure features methods of inducing oocyte maturation in vitro, by co-culturing a female subject's oocytes with an ex vivo composition containing a plurality of ovarian support cells (e.g., granulosa cells). Additional methods for administering follicular triggering agents and retrieving oocytes from the female subject are provided. Such methods, compositions, and apparatuses are particularly useful for assisted reproduction technology (ART) procedures.

The present disclosure relates to vestibular supporting cell promoters and uses thereof. The present disclosure provides polynucleotides containing the SLC6A14 promoter, as well as vectors containing the polynucleotides, which can be used to promote expression of transgenes in vestibular supporting cells. The polynucleotides described herein can be operably linked to a transgene, such as a transgene encoding a therapeutic protein, to promote vestibular supporting cell expression of the transgene. The polynucleotides described herein can be operably linked to a therapeutic transgene and used to treat a subject having or at risk of having vestibular dysfunction.

Provided is a device which includes: an acquisition unit which acquires cell information that relates to cells cultured in first culture and culture information that indicates the culture conditions for the first culture; and an estimation unit which estimates the state of a culture medium after the first culture has been performed, on the basis of the cell information and the culture information. The acquisition unit also acquires post-treatment information that indicates the conditions for a post-treatment performed on the culture medium after the first culture has been performed. The estimation unit estimates the state of the culture medium after the first culture and the post-treatment have been performed, on the basis of the cell information, the culture information, and the post-treatment information.

Prosthetic heart valves including a radially expandable annular frame and valvular structure disposed within the frame, as well as methods and delivery apparatus associated therewith, are disclosed. The frame can include commissure-supporting cells for attachment of commissures of the valvular structure to the frame. The commissure-supporting cells can be offset from an outflow end of the frame by an offset distance and aligned with gaps in the frame. In some examples, the gaps are defined by a row of discontinuous cells in an outflow portion of the frame. In some examples, the gaps are defined by pairs of cells in an outflow portion of the frame. In some examples, the pairs of cells are enlarged relative to other cell of the frame.

This invention provides a method to improve age-related decline in ovarian function by supplementing quiescent oocytes within primordial follicles. [Solution] The present invention provides a method for inducing mature oocytes, comprising the steps of: preparing a single-cell suspension from the ovary of a mammal other than a human; separating the single-cell suspension into oocytes and ovarian somatic cells (supporting cells) within a primordial follicle using a flow cytometer; re-aggregating the oocytes and ovarian somatic cells within the separated primordial follicle; and maturing the re-aggregated primordial follicle in vitro.

A method for performing gene therapy and regeneration of cochlear supporting cells. Specifically, provided is a cochlear-supporting-cell-specific promoter as shown in SEQ ID NO: 1, comprising: (1) an enhancer sequence as shown in SEQ ID NO: 2 or a sequence having at least 90% sequence identity thereto and retaining the function of SEQ ID NO: 2; and (2) a core promoter element SEQ ID NO: 3. The promoter specifically drives the expression of a transgene in the cochlear supporting cells.

Provided is an expression cassette for expressing GJB2 protein, which comprises a cochlear supporting cell and cochlear lateral wall-specific promoter and a nucleic acid encoding GJB2 protein. Also provided is a recombinant adeno-associated viral vector, the genome of which comprises an expression cassette for expressing GJB2 protein inserted between two ITR sequences; and a combination of at least two recombinant adeno-associated viral vectors. The recombinant adeno-associated viral vector or combination thereof can be used for treating or preventing hearing loss associated with defects in the GJB2 gene.

The present disclosure relates to compositions and methods for the conversion of source cells (such as cochlear support cells) to cochlea hair cells and / or cochlea hair cell-like cells by introducing transcription factors (TFs) into the source cells, and methods for treating hearing loss.

The invention provides a separation method and an identification method of bactrian camel testis interstitial cells, and belongs to the technical field of cell separation. The method comprises the following steps: mixing 0.08-0.12% of collagenase IV solution and 0.23-0.27% of trypsin-EDTA (Ethylene Diamine Tetraacetic Acid) according to a volume ratio of 1: 2, digesting a bactrian camel testis tissue block, then carrying out hypotonic treatment purification to remove redundant germ cells, carrying out differential adhesion purification to remove a large amount of supporting cells, carrying out percoll density gradient centrifugation purification to remove a small amount of supporting cells, and carrying out freeze drying to obtain a finished product. And transferring the suspension with the percoll gradient of 34-60% into a new culture bottle to obtain the interstitial cells with the purity of more than or equal to 95%, and completing identification by combining morphological observation, immunofluorescence staining and secretion function detection. The separated bactrian camel testis interstitial cells can be used for double-hump reproductive performance research, and a foundation is laid for the reproductive performance of bactrian camels.

The present disclosure provides polynucleotides containing regions of the TMPRSS3 promoter that are capable of expressing a transgene specifically in cochlear hair cells and sensory support cells. The present disclosure also provides nucleic acid vectors containing these promoters operably linked to polynucleotides encoding polypeptides. The present disclosure provides compositions and methods for inducing transgene expression specifically in inner ear cells such as hair cells and sensory support cells. The compositions described herein can be administered to a subject to treat disorders caused by dysfunction of inner ear cells, such as hearing loss or vestibular dysfunction.

Provided herein are compositions that include at least two different nucleic acid vectors that, when introduced into a primate cell, the at least two different vectors undergo concatamerization or homologous recombination with each other, thereby forming a recombined nucleic acid that encodes a full-length target protein (e.g., a supporting cell target protein or a hair cell target protein). Also provided are compositions that include a single AAV vector that, when introduced into a primate cell, a nucleic acid encoding a full-length target protein (e.g., a supporting cell target protein or a hair cell target protein) is generated at the locus of the supporting cell target gene, and the primate expresses the target protein (e.g., a supporting cell target protein or a hair cell target protein).

This disclosure features methods and compositions for the in vitro maturation of oocytes. In particular, this disclosure features methods and compositions relating to the manipulation of multiple ovarian supporting cells (OSCs) to promote the maturation of oocytes in a culture medium. Such methods and compositions are particularly useful in assisted reproductive technology (ART) procedures.

The invention discloses a GJB2 promoter sequence as well as a vector and application thereof. The promoter sequence is any one of the following nucleotide sequences: 1) a sequence SEQ ID NO.1 in a sequence table; and 2) a DNA sequence which has more than 85% of homology with the sequence SEQ ID NO.1 in the sequence table and has the same function with the sequence SEQ ID NO.1 in the sequence table. The sequence provided by the invention is operably linked to a target gene to drive the specific expression of the target gene in an inner ear supporting cell, plays an important role in otology research, particularly in inner ear gene therapy, can be used for treating subjects suffering from hearing loss or vestibular dysfunction or at risk of hearing loss or vestibular dysfunction, and can be used for treating the subjects suffering from hearing loss or vestibular dysfunction. Wide application prospects and clinical values are realized.

The invention relates to the technical field of micro-fluidic organ chips, and discloses a micro-fluidic testis organ chip and a culture method, the micro-fluidic testis organ chip comprises a PDMS fluid control layer and a PDMS cell culture layer from top to bottom; the at least three culture units are arranged on the PDMS cell culture layer in the axial direction, each culture unit internally comprises three functional areas, the three functional areas are used for testicular sertoli cell culture, spermatogenic cell culture and basement membrane simulation respectively, and the three functional areas are communicated with one another to form a fluid path; the regulation and control part is arranged on the PDMS fluid control layer, and the regulation and control part is used for regulating and controlling fluid paths which are communicated with one another among the three functional areas; the inoculation and collection part is arranged on the PDMS fluid control layer and is used for carrying out cell inoculation and collection in the three functional areas. The in-vivo testis spermatogenesis microenvironment is highly simulated, the limitation of a traditional 2D cell experiment and an animal experiment can be effectively made up, and a new method and platform are provided for analyzing a spermatogenesis disorder mechanism and screening targeted repair drugs.

A nasal tissue implant for reconstruction and tissue engineering of nasal tissue in a subject includes a tissue scaffold component comprising a biocompatible polymeric material having a plurality of open pores configured to support cell growth. The tissue scaffold component conforms to a portion of the subject's nasal region and defines at least a portion of the subject's nasal anatomy. A method of making an implantable nasal tissue implant for reconstructing a portion of a nasal anatomy of a human or other animal subject is also provided that includes laser sintering or three-dimensional (3D) printing a biocompatible polymeric material to form a tissue scaffold component comprising a biocompatible polymeric material having a plurality of open pores configured to support cell growth. Again, the tissue scaffold component substantially conforms to a nasal region specific to the human or other animal subject.