22 results about "Supporting cell" patented technology

Hydrogels comprising a macromolecular matrix and water may be used to augment soft tissue of a human being, promote or support cell or tissue viability or proliferation, create space in tissue, and for other purposes. A macromolecular matrix may comprise a hyaluronic acid component crosslinked to a silk fibroin component.

Featured are methods, compositions, and apparatuses for the in vitro maturation of oocytes. In particular, the disclosure features methods of inducing oocyte maturation in vitro, by co-culturing a female subject's oocytes with an ex vivo composition containing a plurality of ovarian support cells (e.g., granulosa cells). Additional methods for administering follicular triggering agents and retrieving oocytes from the female subject are provided. Such methods, compositions, and apparatuses are particularly useful for assisted reproduction technology (ART) procedures.

Provided is a device which includes: an acquisition unit which acquires cell information that relates to cells cultured in first culture and culture information that indicates the culture conditions for the first culture; and an estimation unit which estimates the state of a culture medium after the first culture has been performed, on the basis of the cell information and the culture information. The acquisition unit also acquires post-treatment information that indicates the conditions for a post-treatment performed on the culture medium after the first culture has been performed. The estimation unit estimates the state of the culture medium after the first culture and the post-treatment have been performed, on the basis of the cell information, the culture information, and the post-treatment information.

Prosthetic heart valves including a radially expandable annular frame and valvular structure disposed within the frame, as well as methods and delivery apparatus associated therewith, are disclosed. The frame can include commissure-supporting cells for attachment of commissures of the valvular structure to the frame. The commissure-supporting cells can be offset from an outflow end of the frame by an offset distance and aligned with gaps in the frame. In some examples, the gaps are defined by a row of discontinuous cells in an outflow portion of the frame. In some examples, the gaps are defined by pairs of cells in an outflow portion of the frame. In some examples, the pairs of cells are enlarged relative to other cell of the frame.

This invention provides a method to improve age-related decline in ovarian function by supplementing quiescent oocytes within primordial follicles. [Solution] The present invention provides a method for inducing mature oocytes, comprising the steps of: preparing a single-cell suspension from the ovary of a mammal other than a human; separating the single-cell suspension into oocytes and ovarian somatic cells (supporting cells) within a primordial follicle using a flow cytometer; re-aggregating the oocytes and ovarian somatic cells within the separated primordial follicle; and maturing the re-aggregated primordial follicle in vitro.

Provided is an expression cassette for expressing GJB2 protein, which comprises a cochlear supporting cell and cochlear lateral wall-specific promoter and a nucleic acid encoding GJB2 protein. Also provided is a recombinant adeno-associated viral vector, the genome of which comprises an expression cassette for expressing GJB2 protein inserted between two ITR sequences; and a combination of at least two recombinant adeno-associated viral vectors. The recombinant adeno-associated viral vector or combination thereof can be used for treating or preventing hearing loss associated with defects in the GJB2 gene.

This disclosure features methods and compositions for the in vitro maturation of oocytes. In particular, this disclosure features methods and compositions relating to the manipulation of multiple ovarian supporting cells (OSCs) to promote the maturation of oocytes in a culture medium. Such methods and compositions are particularly useful in assisted reproductive technology (ART) procedures.

The invention relates to the technical field of micro-fluidic organ chips, and discloses a micro-fluidic testis organ chip and a culture method, the micro-fluidic testis organ chip comprises a PDMS fluid control layer and a PDMS cell culture layer from top to bottom; the at least three culture units are arranged on the PDMS cell culture layer in the axial direction, each culture unit internally comprises three functional areas, the three functional areas are used for testicular sertoli cell culture, spermatogenic cell culture and basement membrane simulation respectively, and the three functional areas are communicated with one another to form a fluid path; the regulation and control part is arranged on the PDMS fluid control layer, and the regulation and control part is used for regulating and controlling fluid paths which are communicated with one another among the three functional areas; the inoculation and collection part is arranged on the PDMS fluid control layer and is used for carrying out cell inoculation and collection in the three functional areas. The in-vivo testis spermatogenesis microenvironment is highly simulated, the limitation of a traditional 2D cell experiment and an animal experiment can be effectively made up, and a new method and platform are provided for analyzing a spermatogenesis disorder mechanism and screening targeted repair drugs.

The disclosure provides SL26A4 enhancers and SLC26A4 promoters, as well as vectors containing the same, that can increase gene expression in SLC26A4-expressing cells, such as interdental cells, root cells, spiral prominence cells, and vestibular supporting cells. The SLC26A4 enhancers and SLC26A4 promoters described herein may be operably linked to a polynucleotide, such as a transgene, encoding an expression product and used for the treatment of subjects having or at risk of developing hearing loss or vestibular dysfunction.

The present disclosure provides polynucleotides containing a TMPRSS3 promoter of a region capable of specifically expressing a transgene in cochlear hair cells and sensory supporting cells. The disclosure also provides nucleic acid vectors containing these promoters operably linked to a polynucleotide encoding a polypeptide. The present disclosure provides compositions and methods for specifically inducing transgene expression in inner ear cells, such as hair cells and sensory supporting cells. The compositions described herein can be administered to a subject to treat a condition caused by inner ear cell dysfunction, such as hearing loss or vestibular dysfunction.

The present disclosure provides polynucleotides and nucleic acid vectors containing a GJB2 regulatory construct operably linked to a polynucleotide encoding a gap junction protein beta2 (Gjb2) protein (e.g., a wild-type human Gjb2 protein). These polynucleotides and vectors can be used to express Gjb2 in cells expressing GJB2, including cochlear supporting cells, and thus can be used to treat subjects suffering from or at risk of developing GJB2-related hearing loss.

The application belongs to the technical field of gene therapy, and particularly relates to an AAV capsid protein mutant targeting glial cells and application thereof. The AAV capsid protein mutant is constructed by inserting a polypeptide with glial cell infection characteristics between N589 and R590 of an AAV-DJ capsid protein amino acid sequence, and exhibits enhanced transduction and targeting in inner ear basal membrane supporting cells, and has the function of enhancing supporting cell transduction in the cochlear basal membrane of newborn and adult mice, thereby being capable of solving the problem of low transduction efficiency of AAV in the cochlear basal membrane supporting cells of adult mice, and providing a potentially available tool for gene therapy of deafness genes positioned in supporting cells by Chen et al. GJB2 and the like.

This invention relates to the fields of environmental toxicology and organoid technology, and particularly to a method for assessing the toxicity of environmental microplastic exposure based on testicular organoids. The method includes: processing waste testicular tissue into a single-cell suspension, the single-cell suspension comprising supporting cells, germ cells, and interstitial cells; seeding the single-cell suspension onto a biomimetic hydrogel scaffold for culture to obtain mature testicular organoids, which are then cultured in a target microplastic exposure solution to establish a microplastic exposure model; determining the raw values ​​of toxicity assessment indicators corresponding to the microplastic exposure model, including: morphological score data, grayscale values ​​of cell marker expression, DNA damage indicators, testosterone secretion levels, and cytotoxicity indicators; calculating a comprehensive toxicity score based on the raw values ​​of each toxicity assessment indicator, and assessing the reproductive toxicity risk level of the target microplastic exposure based on the comprehensive toxicity score. This application can improve the accuracy of assessing the reproductive toxicity risk level of microplastic exposure.

The invention provides a mononuclear transcriptomics technology-based analysis method for interaction of testicular sertoli cells and germ cells of plateau livestock, and belongs to the technical field of bioinformatics. The analysis method comprises the following steps: carrying out mononuclear transcriptome sequencing by taking testis tissues of plateau livestock at different development stages as materials, dividing a supporting cell subset and a germ cell subset according to marker genes, drawing a quasi-time differentiation track, screening differential expression genes and enriching functions, and constructing a supporting cell and germ cell interaction molecular network. According to the method, molecular characteristics, differentiation tracks and interaction networks of supporting cells and germ cells of the testis of the Tibetan sheep in different development stages are analyzed, and the problems that in the prior art, cell resolution is insufficient, development dynamics are deficient, variety specificity information is deficient and the like are solved; a brand new technical approach and theoretical basis are provided for clarification of cell molecular basis of low reproductive capacity of Tibetan sheep and mining of key regulatory factors.

The application belongs to the field of animal reproductive biotechnology, and discloses application of support cell exosomes in promoting in-vitro culture of pig testis tissue, the in-vitro culture comprising: placing pig testis tissue pieces on a three-dimensional culture support and culturing by using a sequential medium system; wherein the sequential medium system comprises M1 medium used for initial culture and M2 medium used for subsequent culture, the M1 medium comprising StemPro-34 SFM basic medium, and the M2 medium comprising alpha-MEM basic medium. The technical problems of poor in-vitro culture effect of testis tissue and difficulty in maintaining the spermatogenesis process in the prior art are solved.

The invention relates to a pillow inner supporting system and method based on a self-adaptive microarray. According to the system, a dense array is formed by supporting units with consistent structures, and each unit can be independently compressed only in the vertical direction. When the head is in contact, the array is autonomously reconstructed into a three-dimensional supporting curved surface accurately matched with the contour of the head and the neck from a unified plane through differential vertical deformation generated by each unit in response to pressure distribution, and therefore real personalized self-adaptive supporting is achieved.

This invention provides a method for isolating and identifying interstitial cells from Bactrian camels, belonging to the field of cell isolation technology. A mixture of 0.08–0.12% collagenase IV solution and 0.23–0.27% trypsin-EDTA at a volume ratio of 1:2 is used to digest Bactrian camel testicular tissue blocks. The mixture is then purified by hypotonic treatment to remove excess germ cells, differential adhesion purification to remove a large number of supporting cells, and Percoll density gradient centrifugation to remove a small number of supporting cells. The suspension, obtained from a 34%–60% Percoll gradient, is transferred to a new culture flask to obtain interstitial cells with a purity ≥95%. Identification is performed using morphological observation, immunofluorescence staining, and secretory function testing. The interstitial cells isolated from Bactrian camels using this invention can be used for research on the reproductive performance of Bactrian camels, laying the foundation for understanding the reproductive performance of Bactrian camels.

This invention provides a composition for treating ovarian insufficiency (POF) and its application. The composition comprises two synergistic modules: first, hiPSC-derived ovarian support cells (iPSC-OSCs) pre-adapted to FTY720 and possessing enhanced oxidative stress tolerance; second, reactive oxygen species (ROS)-responsive dual-targeting lipid nanoparticles (RRDT-LNPs) loaded with a therapeutic agent (such as urestatin A). The RRDT-LNPs are functionalized from a ROS-responsive material containing a thioketal (TK) linker, a primary targeting peptide CNGRC, and a secondary dual-targeting fusion protein VHH-FP. This invention also provides a method for the directed differentiation and pre-adaptation of the iPSC-OSCs, and a method for preparing the RRDT-LNPs. In vitro and in vivo experiments have demonstrated that the composition of this invention, through the organic combination of "cell empowerment" and "intelligent targeted drug delivery," can sequentially activate targeting capabilities in the pathological microenvironment, efficiently repairing ovarian function, restoring endocrine levels, and exhibiting good safety, providing a novel synergistic treatment option for POF.

Featured are methods and compositions for production of ovarian support cells. In particular, the disclosure features methods of culturing or producing ovarian support cells from pluripotent stem or progenitor cells. The ovarian support cells described herein may be used for various purposes, including the treatment of ovarian decline, infertility, and a wide array of other reproductive and gynecologic conditions, as well as for modeling particular components of the human reproductive system in order to identify and characterize new therapeutic interventions.

The invention discloses a preparation for improving the reproductive performance of breeder cocks, a preparation method and application, the preparation for improving the reproductive performance of breeder cocks comprises a fermentation main material, a fermentation auxiliary material and probiotics; the fermentation main materials comprise sophora flower buds, ginkgo leaves and astragalus membranaceus; the fermentation auxiliary materials comprise corn flour and carrot powder; the probiotics comprise bacillus subtilis, enterococcus faecium and saccharomyces cerevisiae. The breeding cocks are fed with the preparation for improving the reproductive performance of the breeding cocks, and the reproductive performance of the breeding cocks can be improved; the composition also can promote spermatogenesis, can promote proliferation and development of sertoli cells of a breeder cock body, plays a positive role as a cell proliferation and development regulating agent, and also can guarantee and maintain the normal form of sperms.