5 results about "Ultrastructure" patented technology

Methods and systems for physical expansion and imaging of biological samples are described herein. In one aspect of the disclosure, a method for preparing a biological sample for the purpose of generating images of its ultrastructure with an imaging instrument includes a) physically expanding the sample by at least a factor of two in at least one dimension; and b) bulk labeling a plurality of components of the sample with at least one reagent to introduce contrast.

The present invention provides a method for labeling a biological tissue by immuno-electron microscopy, and specifically, the present invention provides a method for performing immuno-electron microscopy labeling on a tissue sample. The immuno-electron microscopy labeling is pre-embedding immuno-electron microscopy labeling, and the immuno-electron microscopy labeling is performed under the action of an electric field. Compared with a conventional antibody molecule diffusion-based method (referred to as a passive immuno-labeling method), the present invention solves the problem of an excessively long labeling time in pre-embedding immuno-electron microscopy technology by means of an external electric field-assisted antibody molecule diffusion technique (referred to as an active immuno-labeling method). Rapid immuno-electron microscopy labeling can be implemented, and an ultrastructure of a biological tissue can be protected.

The application provides a method and system for predicting the damage of testicular supporting cells by vomitoxin, and relates to the technical field of testicular supporting cell damage prediction, which comprises obtaining transcriptome sequencing, immunofluorescence quantification, electron microscope ultrastructure and flow detection data; based on the theory of persistent coherence, the topological characteristics of the gene expression space are extracted and encoded into persistent bar code representation; through a neural differential equation, the bar code is fused with protein and structure data for multi-modal fusion and dynamic evolution modeling, and topological kinetic characteristics representing barrier function are obtained; combined with flow data, a cell state phase transition model is constructed by applying a cellular automaton and percolation theory to simulate the critical damage threshold of the blood-testis barrier integrity; a potential well model is constructed based on the threshold and flow data; finally, the path integral and variation method are used to calculate the damage probability of cells under different toxin concentrations. The application realizes early warning and dynamic risk prediction of testicular supporting cell damage by vomitoxin.

The invention provides an AIE photosensitizer with a mitochondrial targeting function as well as a preparation method and application of the AIE photosensitizer. The AIE photosensitizer has the following structure shown in the specification. Under the action of illumination, TTP-Mito can induce toxoplasma gondii to generate a large amount of intracellular ROS and cause typical ultrastructure damage such as membrane rupture and collapse, and rapid inactivation of free polypide bodies is realized. Meanwhile, the area of plaques formed by the toxoplasma gondii in host cells can be remarkably reduced, it is proved that the compound can effectively inhibit invasion, replication and amplification capacity of the toxoplasma gondii bodies, and the compound has definite in-vitro toxoplasma gondii resisting activity.

The present application relates to the technical field of cell imaging, and particularly relates to a method for observing the surface structure of the nucleus of a cell. The method comprises nuclear-cytoplasmic separation and observation of the nucleus. The nuclear-cytoplasmic separation comprises cell pretreatment and centrifugal separation of cytoplasm and nucleus. The cell pretreatment comprises placing the cell in a culture medium comprising nocodazole and cytochalasin B for 1.5-3 hours; the culture medium comprises, in terms of weight parts, 0.1-0.5 parts of nocodazole and 2-10 parts of cytochalasin B. The present application provides a nuclear-cytoplasmic separation method for treating cells with nocodazole and cytochalasin B, which can quickly obtain the nucleus while improving the problems of nucleus shrinkage and collapse, and completely preserving the structure of the nucleus, which has important value in the field of observing the surface ultrastructure of the nucleus.