21 results about "Ultrastructure" patented technology

The invention discloses a multi-effect ultrastructure composite lens for preventing and delaying myopia, which comprises a lens main body, a functional area is arranged in the lens main body, and the functional area comprises a central optical correction area and an ultrastructure area which are arranged on the inner side. Compared with the prior art, the method has the advantages that the small central optical area is adopted to correct vision, and the prevention and control effect is enhanced. The ultramicro soft light area has prevention and control and visual transition effects. The ultra-micro lenses and the ultra-micro structure ring cylindrical lenses are alternately arranged on the outer side, due to the fact that the area is reduced, the number of the ultra-micro structures in the unit area is increased, the filling rate of the functional area of the whole lens is increased, the ultra-micro structures enable the brain to be not easy to perceive, visual perception is not easy to interfere, and the three ultra-micro structures have the effect of improving visual activity along with eyeball movement of visual activity. Signals entering the eyes are kept in dynamic change all the time, so that the visual experience is further improved while the prevention and control effect and imaging intervention are guaranteed, and long-term wearing is facilitated.

The invention relates to the technical field of optics, and discloses a visualization method of a biological tissue three-dimensional full-information atlas, which comprises the following steps of: acquiring image data, electron microscope data, mechanical data, protein distribution, omics information and histological staining images of biological tissues by using various biological visualization technologies, and correspondingly processing the acquired data to obtain a three-dimensional full-information atlas of the biological tissues; finally, organization information fusion and digital display are achieved. According to the method, a cross-scale feature correlation algorithm framework is innovatively established, and multi-source heterogeneous data fusion is performed on medical image topological data (CT / MRI), ultrastructure scanning data (SEM / TEM), molecular positioning information (immunofluorescence, immunohistochemistry and enzyme immunoassay), omics maps (space transcriptome / proteome) and high-resolution tissue staining data. The data island dilemma caused by traditional single-mode analysis is broken through, and full-scale visualization from nanoscale molecular distribution to centimeter-level organ tissue structure-function relationship is realized for the first time.

The invention relates, in part, to methods and compounds for ultrastructure membrane expansion microscopy (umExM), which permits high-resolution visualization of membrane ultrastructure using light microscopy.

A cryopreservation medium including: a cryoprotective particle or macromolecule; and an aqueous liquid, wherein the cryoprotective particle or macromolecule is hydrophilic and has a spherical shape when dissolved or suspended in the aqueous liquid. A cryopreservation method that uses the medium of the present invention to preserve the ultrastructures of the tissues and simultaneously decellularize the tissues in a simple freezing procedure.

Methods and systems for physical expansion and imaging of biological samples are described herein. In one aspect of the disclosure, a method for preparing a biological sample for the purpose of generating images of its ultrastructure with an imaging instrument includes a) physically expanding the sample by at least a factor of two in at least one dimension; and b) bulk labeling a plurality of components of the sample with at least one reagent to introduce contrast.

The invention relates, in part, to methods and compounds for ultrastructure membrane expansion microscopy (umExM), which permits high-resolution visualization of membrane ultrastructure using light microscopy.

The invention discloses an ultrastructure lens for preventing and delaying myopia, which comprises a lens main body, a functional area is arranged in the lens main body, the functional area comprises a central optical correction area arranged on the inner side, an ultramicro lens area is arranged on the outer side of the central optical correction area on the lens main body, and the ultramicro lens area is arranged on the outer side of the central optical correction area. An annular blank area is arranged on the outer side of the ultra-micro lens area, and the central optical correction area and the ultra-micro lens area form a functional area. Compared with the prior art, the invention has the advantages that: the ultra-micro lens less than 0.3 mm is adopted, and the area is reduced, so that the number of ultra-micro structures in unit area is increased, and the filling rate of the functional area of the whole lens is increased; meanwhile, due to the ultrastructure, the brain is not easy to perceive, and visual perception is not easy to interfere, so that the visual experience is further improved while the prevention and control effect and imaging intervention are ensured, and long-term wearing is facilitated.

The invention relates to a Ruditapes philippinarum blood cell function classification method based on ultrastructure change, and belongs to the technical field of marine biologication.According to the method, blood cell function classification is carried out according to ultrastructure change characteristics, and three dissolved oxygen change modes are set; the three treatment processes are respectively constant normal dissolved oxygen treatment, normal dissolved oxygen-rapid hypoxia-rapid reoxygenation treatment and normal dissolved oxygen-slow hypoxia-slow reoxygenation treatment. And then randomly extracting ruditapes philippinarum blood lymph, quickly fixing the ruditapes philippinarum blood lymph, carrying out blood cell scanning and transmission electron microscope sample preparation and observation, and carrying out functional classification according to the change of a blood cell ultrastructure. The method can overcome the limitation that Ruditapes philippinarum individuals are small, various dissolved oxygen changes in a natural water area are simulated, the activity state of blood cells responding to environmental changes and the normal reaction form of the blood cells in an organism are preserved to the maximum extent, operation is easy and convenient, the requirements of repeated experiments and contrast experiments can be met, and the method is suitable for popularization and application. And the functional classification of the ruditapes philippinarum blood cells is accurately realized.

The invention provides a transmission electron microscope sample preparation method of a sample to be positioned based on a novel embedding medium, which effectively improves the toughness of an embedding block, improves the quality of an ultrathin section of the sample and reduces the conditions of falling and cracking of the ultrathin section by researching the optimal formula of an SPI-PON 812R embedding mixture, optimizing the stirring time and combining with a programmed heating polymerization method. The requirement for semi-thin positioning and slicing of the sample is met, the integrity of the target structure of the sample is guaranteed, the problems that the semi-thin positioning of the kidney tissue and other samples embedded by the novel embedding medium is difficult, the imaging effect is not ideal and the like are effectively solved, a solution is provided for further application and popularization of the novel embedding medium 812R, and the application prospect is wide. The method can be used for preparing high-quality electron microscope samples of human or mouse kidney tissues and the like, so that ultrastructure observation and pathological diagnosis become possible.

Methods and systems for physical expansion and imaging of biological samples are described herein. In one aspect of the disclosure, a method for preparing a biological sample for the purpose of generating images of its ultrastructure with an imaging instrument includes a) physically expanding the sample by at least a factor of two in at least one dimension; and b) bulk labeling a plurality of components of the sample with at least one reagent to introduce contrast.

The invention provides a preparation method of a transmission electron microscope brain tissue sample. The preparation method comprises the steps of glutaraldehyde pre-immobilization, normal saline rinsing, osmium pollution inhibition stationary liquid immobilization, deionized water rinsing, gradient dehydration, gradient permeation and the like. The osmium pollution inhibition stationary liquid is prepared by mixing an osmium tetroxide aqueous solution with the mass fraction of 2-4% and a potassium ferrocyanide aqueous solution with the mass fraction of 2-4% according to the volume ratio of 1: (1-1.5); the embedding resin composition is composed of epoxy resin 618, dodecyl succinic anhydride and dibutyl phthalate, and the volume ratio of the epoxy resin 618 to the dodecyl succinic anhydride to the dibutyl phthalate is (26-27): (18-19): 5. The preparation method disclosed by the invention can effectively inhibit osmium pollution and improve the imaging quality of a brain tissue sample, and is suitable for observing the ultrastructure of the brain tissue under a transmission electron microscope.

The present invention provides a method for labeling a biological tissue by immuno-electron microscopy, and specifically, the present invention provides a method for performing immuno-electron microscopy labeling on a tissue sample. The immuno-electron microscopy labeling is pre-embedding immuno-electron microscopy labeling, and the immuno-electron microscopy labeling is performed under the action of an electric field. Compared with a conventional antibody molecule diffusion-based method (referred to as a passive immuno-labeling method), the present invention solves the problem of an excessively long labeling time in pre-embedding immuno-electron microscopy technology by means of an external electric field-assisted antibody molecule diffusion technique (referred to as an active immuno-labeling method). Rapid immuno-electron microscopy labeling can be implemented, and an ultrastructure of a biological tissue can be protected.

The invention provides a method and a system for predicting vomitoxin damaged testicular sertoli cells, and relates to the technical field of testicular sertoli cell damage prediction.The method comprises the following steps: obtaining transcriptome sequencing, immunofluorescence quantification, electron microscope ultrastructure and flow detection data; extracting topological features of a gene expression space based on a continuous homology theory and encoding the topological features into continuous bar codes for expression; performing multi-modal fusion and dynamic evolution modeling on the bar code, the protein and the structural data through a neural differential equation to obtain topological dynamic characteristics representing the barrier function; constructing a cell state phase change model by combining flow data and applying a cellular automaton and a seepage theory, and simulating to obtain a critical damage threshold of the integrity of the blood testis barrier; constructing a potential well model based on the threshold value and the streaming data; and finally, calculating the damage probability of the cells under different toxin concentrations by adopting path integration and variational methods. According to the method, early warning and dynamic risk prediction of the testicular sertoli cells damaged by the vomitoxin are realized.

The present invention discloses a platelet ultrastructure imaging detection sample processing reagent, comprising a diluent; the ratio of the diluent, blood preservation solution and benchtop solution is 1:7 to 12. The present invention also provides a platelet ultrastructure imaging detection sample processing kit, comprising the sample processing reagent of the present invention, preferably also including a fixative, the fixative comprising 4% to 8% paraformaldehyde by volume, and the solvent comprising a PHEM buffer solution with a pH of 6.8 to 7.2, wherein the platelet microtubule structure fixative preferably comprises 5.5% to 6.6% paraformaldehyde, 0.05% to 0.15% glutaraldehyde and 0.1% to 0.2% TritonX-100 by volume. In addition, the present invention also provides a platelet ultrastructure imaging detection sample processing reagent for use in preparing imaging detection samples, wherein the diluent is first added to the platelet plasma obtained by centrifugation, and the platelets are then fixed after being allowed to stand for recovery after dilution, thereby obtaining unactivated platelets, so that the test results are closer to the true condition of platelets under physiological conditions.

The invention belongs to the technical field of medicines, and discloses a qi deficiency and blood stasis model and application thereof. A mouse qi deficiency and blood stasis model is prepared by combining temporary occlusion of bilateral common carotid artery with systemic hypotension surgery and sleep deprivation, and the model is detected through gripping power, lingual surface image analysis, open field behavioristics, new object recognition experiments, electron microscope ultrastructure analysis and functional nuclear magnetism. The results of ultrastructure and functional nuclear magnetism show that the model conforms to the clinical common qi deficiency and blood stasis characterization such as weakness, weakness, white tongue, memory deterioration and the like, and the model conforms to the clinical actual pathological conditions of qi deficiency and blood stasis, and is a qi deficiency and blood stasis syndrome. And a reliable animal model is provided for screening medicines and health-care products for preventing and recovering ischemic stroke.

The application provides a method and system for predicting the damage of testicular supporting cells by vomitoxin, and relates to the technical field of testicular supporting cell damage prediction, which comprises obtaining transcriptome sequencing, immunofluorescence quantification, electron microscope ultrastructure and flow detection data; based on the theory of persistent coherence, the topological characteristics of the gene expression space are extracted and encoded into persistent bar code representation; through a neural differential equation, the bar code is fused with protein and structure data for multi-modal fusion and dynamic evolution modeling, and topological kinetic characteristics representing barrier function are obtained; combined with flow data, a cell state phase transition model is constructed by applying a cellular automaton and percolation theory to simulate the critical damage threshold of the blood-testis barrier integrity; a potential well model is constructed based on the threshold and flow data; finally, the path integral and variation method are used to calculate the damage probability of cells under different toxin concentrations. The application realizes early warning and dynamic risk prediction of testicular supporting cell damage by vomitoxin.

A feature extraction method and system based on platelet ultrastructure imaging. The feature extraction method comprises: acquiring stable platelets having retained activity, and performing anticoagulation treatment on the platelets; performing antibody labeling on the platelets so as to prepare a sample for platelet imaging; imaging the sample so as to obtain a platelet ultrastructure picture; and segmenting the platelet ultrastructure picture to obtain individual-platelet pictures, acquiring diameter information of the corresponding platelets, and acquiring in the individual-platelet pictures the numbers of dense granules and α granules, granule area information, subcellular distribution information and α granule subcellular distribution features of activated platelets, so as to achieve feature extraction of platelet ultrastructures. The feature extraction method and system based on platelet ultrastructure imaging can acquire contour features of platelets, also extract the subcellular structural features of α granules and dense granules of platelets to serve as biomarkers, thus achieving important significance for the research and testing of physiological functions of platelets.

The invention provides an AIE photosensitizer with a mitochondrial targeting function as well as a preparation method and application of the AIE photosensitizer. The AIE photosensitizer has the following structure shown in the specification. Under the action of illumination, TTP-Mito can induce toxoplasma gondii to generate a large amount of intracellular ROS and cause typical ultrastructure damage such as membrane rupture and collapse, and rapid inactivation of free polypide bodies is realized. Meanwhile, the area of plaques formed by the toxoplasma gondii in host cells can be remarkably reduced, it is proved that the compound can effectively inhibit invasion, replication and amplification capacity of the toxoplasma gondii bodies, and the compound has definite in-vitro toxoplasma gondii resisting activity.

The present application relates to the technical field of cell imaging, and particularly relates to a method for observing the surface structure of the nucleus of a cell. The method comprises nuclear-cytoplasmic separation and observation of the nucleus. The nuclear-cytoplasmic separation comprises cell pretreatment and centrifugal separation of cytoplasm and nucleus. The cell pretreatment comprises placing the cell in a culture medium comprising nocodazole and cytochalasin B for 1.5-3 hours; the culture medium comprises, in terms of weight parts, 0.1-0.5 parts of nocodazole and 2-10 parts of cytochalasin B. The present application provides a nuclear-cytoplasmic separation method for treating cells with nocodazole and cytochalasin B, which can quickly obtain the nucleus while improving the problems of nucleus shrinkage and collapse, and completely preserving the structure of the nucleus, which has important value in the field of observing the surface ultrastructure of the nucleus.

The invention relates to the technical field of medical detection, in particular to a method for evaluating the treatment effect of alveolar echinococcosis, which comprises the following steps: acquiring an echinococcosis multilocularis sample after radiation treatment; determining the mortality rate of the protoscolex through a trypan blue dyeing method; observing the superstructure change of the hair growth layer cells through a transmission electron microscope; detecting mitochondrial DNA copy number change through qPCR (quantitative polymerase chain reaction); the cell apoptosis degree is evaluated through caspase-3 activity detection; the treatment effect is evaluated according to the mortality rate of the protoscolex, the ultrastructure change, the mitochondrial DNA copy number change and the cell apoptosis degree.

The present disclosure relates to novel procedures for cryoprotection of tissue for imaging procedures such as optoelectronic correlation microscopy (CLEM) and immunogold electron microscopy (iEM) as well as fluorescence imaging. The procedure combines perfusion immobilization with cryoprotection of organs or tissues harvested in dimethyl sulfoxide (DMSO) and cryoreplacement in an organic solvent to reliably freeze and treat larger tissue blocks while retaining ultrastructures for image analysis.