74 results about "Ultrastructure" patented technology

The invention discloses biological ink for 3D printing. The biological ink for 3D printing comprises water-soluble synthetic polymer with the cross-linking function, water-soluble natural polymer with the cross-linking function, bioactive components capable of spontaneously forming special ultrastructures, cross-linking initiator and solvents and further comprises bioactive components. The biological ink for 3D printing overcomes the defects that traditional 3d printing ink is single in component structure, does not have good biological activity and needs to utilize organic solvents, and it is difficult to take cell compatibility, biological activity and mechanical properties into consideration, the biological ink which has biological activity and the fast curing function at the same time and can form a certain microstructure is obtained, and hydrogel obtained through curing of the ink has controllable mechanical properties, good structural stability and a good fidelity effect.

A novel cell seeded hollow fiber bioreactor is described as a potential bioartificial kidney. Endothelial cells along with pericyte, vascular smooth muscle, and / or mesangial cells or any mesenchymally derived support cells are seeded along a hollow fiber in a perfused bioreactor to reproduce the ultrafiltration function and transport function of the kidney. Maintenance of tissue specific function and ultrastructure suggest that this bioreactor provides an economical device for treating renal failure.

A method for the evaluation of the ultrastructure of connective tissue, such as cartilage, including (a) providing a probe operative in the near-infrared or mid-infrared region of the electromagnetic spectrum, (b) positioning the probe either to be in contact with the connective tissue (for detecting attenuated total reflectance) or within a sufficient distance from the surface of the connective tissue (for detecting reflection), (c) detecting infrared radiation which penetrates the surface of the connective tissue for detecting attenuated total reflectance or which reflects off the surface of the connective tissue and (d) analyzing the infrared radiation from step (c) for at least one of peak height, peak area and frequency, and comparing at least one of the peak height, the peak area and the frequency for established values for at least one of peak height, peak area and frequency for normal connective tissue to detect a modification in the molecular structure of the connective tissue.

The present invention relates to methods for the labeling of nucleic acid polymers in vitro and in vivo. In particular, the methods include a [3+2] cycloaddition between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent attached to a label. Such methods do not require fixation and denaturation and therefore can be applied to the labeling of nucleic acid polymers in living cells and in organisms. Also provided are methods for measuring cellular proliferation. In these methods, the amount of label incorporated into the DNA is measured as an indication of cellular proliferation. The methods of the invention can be used in a wide variety of applications including clinical diagnosis of diseases and disorders in which cellular proliferation is involved, toxicity assays, and as a tool for the study of chromosomes' ultrastructures.

The invention relates to a preparation method of TEM (transmission electron microscope) ultrathin section samples of phosphorus-accumulating bacteria, which comprises the following steps: after a sample is taken, carrying out centrifugal cleaning on the sample, putting the phosphorus-accumulating bacteria PAOs sample into a glutaraldehyde solution with a pH value being 7.4 and a volume percentage concentration of 2.5% to fix; dyeing the phosphorus-accumulating bacteria sample by using a Pb(NO3)2 solution with a mass percentage concentration of 2-10%; carrying out dehydration by using a gradient ethanol-acetone series dehydration method; and carrying out resin impregnation and embedding, and sectioning finally. The method disclosed by the invention can be used for solving the problem that, the sample pretreatment process is complicated, the toxicity of used medicaments is strong, and the ultrastructure in a PAOs cell is difficult to clearly display in existing methods; and once the preparation method is adopted for preparing PAOs samples, in TEM observation, the cell structures of bacteria, especially poly-Ps in bacteria, can be clearly and completely revealed.

The invention relates to a noninvasive sperm form and ultrastructure analysis method. The method comprises the following steps: analyzing by using a dispersion type confocal Raman spectrometer, selecting an excitation Raman spectrum, placing sperm cells under a reflected light microscopy, and acquiring the chemical group spectrum of the sperm cells through a certain excitation power and a certain exposure time; respectively dividing the acrosome, the neck and the tail of a sperm into scanning areas, scanning three times to obtain a Raman data spectrum peak, and analyzing the waveform change and the displacement of the spectrum peak; and retrieving the acquired sperm form Raman spectrums in an RRUFF Raman database to obtain the Raman spectra of all areas of the sperm form. The laser Raman spectrum adopted in the invention is a noninvasive technology, so the characteristics of the ultrastructures of different individuals and the scattering substances of different components of each sperm can be reflected without any dye or fixation, and a new assessment method is provided for the sperm morphology analysis.

The invention discloses an ecological Australia murray cod ichthyophthiriusmultifiliis preventing technology. When the ichthyophthiriusmultifiliis occurs on Australia murray cod, sea water is added into a water body where the Australia murray cod is located, detecting is conducted timely through a salinity meter, the salinity is controlled to be 8 thousandths to 10 thousandths, soaking is conducted for 48h, and the ichthyophthiriusmultifiliis is killed. After conducting some deep and basic research on the ultrastructure, the infection mechanism, pathogenesis and immunoreaction and the like of the ichthyophthiriusmultifiliis, the fact that a life cycle of the ichthyophthiriusmultifiliis is divided into stages of trophozoite, cyst and predation is found out. An ichthyophthiriusmultifiliis body is weak during the predation stage and can be restrained effectively by changing the environment, bad effects of pesticide on fish can be avoided, and meanwhile the ichthyophthiriusmultifiliis can be killed thoroughly and effectively.

The invention discloses a construction method of an in-vitro model for simulating mesenchymal stem cell transplantation, which comprises the following steps of: (1) selecting in-vitro neonatal rat cortical neurons; (2) selecting in-vitro rat mesenchymal stem cells; (3) pre-processing the in-vitro rat mesenchymal stem cells, and inoculating in the neonatal rat cortical neurons for co-culture; and (4) after the co-culture, treating the rat mesenchymal stem cells and the neonatal rat cortical neurons by a light microscope, a fluorescence microscope, an atomic force microscope and a scanning electron microscope; and determining an in-vitro model for simulating mesenchymal stem cell transplantation and a growth tendency, an interaction and formation of a synapsis-like structure between the cortical neurons and the mesenchymal stem cells on an ultrastructure. The construction method disclosed by the invention provides a simplified in-vitro model for the mesenchymal stem cell transplantation; meanwhile, the synapsis-like structure formed in the model provides an in-vitro evidence of a structure integration mechanism for the mesenchymal stem cell injury mechanism.

The invention provides a quick and simple leafhopper digestive organ anatomy method and belongs to the field of entomotomy. According to the method, a complete leafhopper digestive tract can be obtained in a short time, losses of components of cells can be reduced to the greatest degree, it can be guaranteed that initial conditions of ultrastructures in the cells can be kept complete, and good results in the next step of experiment can be obtained conveniently. According to the method, the digestive tract is connected with salivary glands of the head and the rectum of the tail, the digestive tract can be dissected out carefully through a drawing and pulling method, digestive organs are not damaged, leafhopper shells are not destroyed, and the completeness of insects are reserved to the greatest degree.

The invention provides an application of a pharmaceutical composition in the preparation of drugs for improving cerebral microcirculation dysfunction. The application uses an ischemia-reperfusion model which is established by the ligation of bilateral carotid arteries of a Mongolian gerbil, the dynamic cerebral microcirculation including cerebral blood flow, blood velocity, blood vessel diameter, generation of vascular endothelial peroxides, leukocyte adhesion and change of vascular permeability of the Mongolian gerbil is observed through a visual dynamic continuous system after the ligation of the bilateral carotid arteries, and a projecting and scanning electron microscope is further used for observing the changes of ultrastructures of small veins and capillaries for observation and research, thereby proving that Yangxueqingnao particles have the effects of treating and improving the cerebral microcirculation dysfunction caused by ischemia-reperfusion.

The invention discloses a traditional Chinese medicine preparation for assisting acupuncture treatment of diabetic gastroparesis and a preparation method and applications thereof. The traditional Chinese medicine preparation is prepared from the following raw materials in parts by weight: 8-10 parts of astragalus membranaceus, 8-10 parts of codonopsis pilosula, 8-10 parts of atractylodes macrocephala koidz, 3-6 parts of fructus amomi, 6-8 parts of hovenia dulcis, 6-8 parts of salvia miltiorrhiza and 6-8 parts of endothelium corneum gigeriae galli. The traditional Chinese medicine preparation is prepared by decocting raw herbal materials with water, then extracting and concentrating. Pharmacodynamic experiments show that the traditional Chinese medicine preparation for assisting acupuncture treatment of diabetic gastroparesis has no obvious adverse reaction, and is safe and reliable; the preparation can be used for promoting the increase of body mass, food intake and stool quantity of mice with diabetic gastroparesis, relieving the gastric electrical activity confusion and improving the gastric antrum ICC ultrastructure very well, so that the expression of positive ICC and SP positive materials of gastric antrum c-kit can be enhanced, and the positive expression of nNOS can be lowered; and the preparation has a good improvement effect on the number increasing, form and signal transduction function of ICC.

The invention discloses an organ chip. The organ chip comprises a No.1 outer-layer chip, a No.1 porous layer is arranged on the outer surface of the lower end of the No.1 outer-layer chip, a middle-layer chip is arranged on the outer surface of the lower end of the No.1 porous layer, and a middle-layer chip is arranged on the outer surface of the lower end of the middle-layer chip. By the aid of the middle-layer chip, the organ chip can simulate tiny structures in human liver organs by a bionic cell culture chamber, a bionic left rear superior border vein, a bionic middle hepatic vein, a bionic left medial lobe vein branch, a bionic left outer lobe vein branch, a bionic caudate lobe vein, a bionic right rear superior border vein, a bionic right hepatic vein, a bionic auxiliary middle hepatic vein, a bionic right anterior lobe vein branch and a bionic right rear upper vein, ensure that cells can better adapt to simulation survival environment, form ultrastructures of liver organs in human bodies and more accurately simulate cell survival environments in human bodies so as to improve medicine detection accuracy and efficiency.

The invention discloses an ultrastructure lens and a manufacturing method thereof. The method comprises the steps that the focus of the ultrastructure lens is determined; a design variable of the surface type of the ultrastructure lens is set in a cross section layer of a cross section assembly, wherein the cross section assembly comprises a substrate, the cross section layer and a perfect matching layer, and excitation waves are distributed inside the cross section assembly; exponential power law mixed material interpolation of a pre-selected material and a vacuum relative dielectric constantin the cross section layer is carried out according to the design variable, and the distribution position of the pre-selected material is determined to obtain the cross section structure configuration of the ultrastructure lens so as to maximize the electric field energy density at the focus, wherein the pre-selected material is the material of the ultrastructure lens, and the cross section structure configuration is rotated along the symmetry axis of the ultrastructure lens to obtain the structure configuration of the ultrastructure lens; and according to the structural configuration, the ultrastructure lens is machined and obtained. The structure configuration is reversely designed according to the required ultrastructure lens, the ultrastructure lens is machined and obtained, the convenience is achieved, the geometric structure data size of the ultrastructure lens is reduced by obtaining the profile structure configuration, and the manufacturing efficiency is improved.

The invention provides a medicine for treating primary osteoporosis through a kidney-tonifying, spleen-strengthening and blood-stasis-removing method. The medicine is prepared from epimedium, antler gum, tortoise-plastron glue, semen cuscutae, cinnamon, rhizoma cibotii, achyranthes roots, eucommia bark, turtle shells, drynaria rhizome, astragalus, teasel roots, dried rehmannia roots, cornus officinalis, Chinese yam, poria cocos, moutan bark, rhizoma alismatis, radix angelica sinensis, white peony roots, radix salvia, leeches, dicranopteris linearis, aster striatus, orixa japonica and raw licorice roots. The medicine can improve the shape of bone trabecula by inhibiting bone loss caused by osteoclast, effectively improves ultrastructures of osteoporotic cancellous bones, increases bone mass, prevents and repairs microscopic fracture, and achieves the curative effect of preventing osteoporosis.