Preparation method of decellularized nerve scaffold and effect evaluation method of preparation method

An evaluation method and decellularization technology, applied in the field of nerve scaffold materials, can solve the problems of inability to fully reflect the decellularization effect of scaffolds and the limitations of detection methods
CN110464877AInactive Publication Date: 2019-11-19BINZHOU MEDICAL COLLEGE
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
BINZHOU MEDICAL COLLEGE
Publication Date
2019-11-19
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a preparation method of a decellularized nerve scaffold and an effect evaluation method of the preparation method. According to the preparation method, the decellularized nervescaffold is prepared by adopting a mode of combining TritonX-100 and a freeze-thaw enzyme. The decellularized effect on the decellularized nerve scaffold is evaluated through ways of morphological observation, ultrastructure observation, cytotoxicity detection, biocompatibility detection, complex frozen section observation and the like. The result shows that the decellularized nerve scaffold which is prepared by adopting a mode of combining the TritonX-100 and the freeze-thaw enzyme has the advantages of sufficient decellularized effect, complete three-dimensional space structure, smooth canals, good compatibility with cells and tissues, slight inflammatory reaction and good cell growth and proliferation state, is suitable for adhesion growth of cells, and is an ideal scaffold material.
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Description

Technical field:

[0001] The invention relates to a preparation method of a decellularized nerve scaffold and an effect evaluation method thereof, belonging to the technical field of nerve scaffold materials. Background technique:

[0002] Spinal cord injury is a common disease with a high disability rate, which not only endangers the life of patients, but also reduces the quality of life of patients. The huge medical expenses bring a heavy economic burden to families and the medical and health system. Therefore, it is extremely urgent to find effective therapeutic measures for spinal cord injury. With the development of tissue engineering, tissue engineering has become a research boom in the treatment of repairing central nervous system injuries. The key is to apply ideal scaffold materials, select suitable seed cells, combine with signal factors that promote cell growth, and construct tissue engineering complexes. . Scaffolds are frame materials that support the growth of...

Claims

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