6 results about "Hirsutella sp." patented technology

The application discloses a kind of bat moth larvae double bacteria synergistic infection methods, belong to microbial engineering and medicinal fungus cultivation technical field, comprising: 1-2 age larvae are preprocessed by containing first probiotic feed feeding, optimize intestinal microenvironment;3-4 age larvae are injected and infected using mixed bacterial liquid containing second probiotic and Chinese hairy hair fungus, promote spore germination and colonization;5 age and above larvae are stably cultivated by third probiotic spraying, whole process is matched with dynamic monitoring and threshold emergency control.The technology adopts age differentiation operation of low-age feeding-middle-age accurate injection-high-age spraying, matches each stage functional targeted probiotic, constructs suitable mycelium germination microenvironment by means of probiotic metabolite, inhibits miscellaneous bacteria pollution and provides adenosine synthesis precursor, realizes the excellent effect of infection rate improvement, mortality reduction, adenosine content improvement and effective content improvement, provides efficient feasible technical path for large-scale high-quality cultivation of cordyceps sinensis.

This invention discloses an induction culture medium and a culture method for isolating *Hypericum sinense* strains from *Cordyceps sinensis* branches. The induction culture medium includes sucrose 5-20 g / L, starch 5-20 g / L, potassium nitrate 1-5 g / L, ammonium nitrate 4-8 g / L, zinc sulfate 5-20 mg / L, glycine 1-5 mg / L, calcium chloride 1-5 g / L, magnesium sulfate 1-2 g / L, ascorbic acid 0.3-0.6 g / L, NAA 0.1-2 mg / L, β-cyclodextrin 5-20 g / L, and agar 20 g / L. The culture method includes: (1) generating aerial mycelia from *Cordyceps sinensis* branches; (2) culturing the aerial mycelia in the induction culture medium to obtain *Hypericum sinense* strains. The cultivation method provided by this invention can isolate the Chinese trichomoniasis strain from broken branches of Cordyceps sinensis, which can reduce or avoid resource waste, lower cultivation costs, is convenient and fast, has a high mycelial germination rate, and a fast strain growth rate, which is conducive to the large-scale promotion of artificial cultivation technology of Cordyceps sinensis.

The invention provides application of hirsutella sinensis polysaccharide in preparation of a neuroprotective agent, and belongs to the technical field of traditional Chinese medicine, and the application comprises the following steps: providing hirsutella sinensis mycelium, extracting the hirsutella sinensis mycelium with water to obtain an extracting solution, concentrating the extracting solution, carrying out alcohol precipitation, and collecting precipitate to obtain crude polysaccharide; purifying the crude polysaccharide through ion exchange chromatography, eluting with sodium chloride with gradient concentration, collecting eluent, concentrating, freeze-drying, carrying out alcohol precipitation, and collecting precipitate to obtain purified hirsutella sinensis polysaccharide; the hirsutella sinensis polysaccharide has an obvious neuroprotective effect on rats suffering from cerebral ischemic stroke caused by middle artery obstruction: the cerebral infarction area is reduced by more than or equal to 35%, the Longa score is reduced by more than or equal to 1.5 score, the bile acid level is improved by more than 4 times, and the content of serum inflammatory factors TNF-alpha, IL-1beta and IL-18 is reduced by more than or equal to 50% in 100mg of middle dose. And a new treatment choice is provided for clinic.

The invention relates to a hirsutella sinensis strain as well as a fermentation method and application thereof, and belongs to the technical field of microorganisms. The strain is separated from a natural cordyceps sinensis sample, and green, high-yield, stable and sustainable Hirsutella sinensis mycelia are obtained through a liquid fermentation process. The strain disclosed by the invention shows excellent growth and proliferation rates in fermentation culture, and the adenosine content of the strain is surprisingly high. In addition, the strain disclosed by the invention shows an excellent bidirectional immunoregulation effect, not only can effectively improve the immune function of macrophages in a resting state, but also has an inhibition effect on excessively activated macrophages.

The present application relates to the technical field of polysaccharide extraction, and particularly relates to a Chinese hairy cap polysaccharide, a preparation method and application thereof. Polysaccharides in the Chinese hairy cap are extracted, separated and purified, and four polysaccharide components are obtained. The four polysaccharides can inhibit HIBEC-EMT, avoid liver damage, and can be used for treating and / or preventing primary biliary cholangitis.

The invention discloses a cordyceps sinensis strain AF-Os2007 and a sporocarp cultivation method of the cordyceps sinensis strain AF-Os2007. The cordyceps sinensis strain AF-Os2007 is classified and named as Hirsutella sinensis, the cordyceps sinensis strain AF-Os2007 is preserved in the China General Microbiological Culture Collection Center on October 10, 2025, and the preservation number of the cordyceps sinensis strain AF-Os2007 is CGMCC No.42272. The cordyceps sinensis strain AF-Os2007 has the advantages that the cordyceps sinensis strain AF-Os2007 can be used for preparing the cordyceps sinensis strain AF-Os2007; the sporocarp cultivation method comprises the following steps: inoculating a preserved cordyceps sinensis strain AF-Os2007 into a PDA solid culture medium for activation culture to obtain an activated strain; sequentially carrying out shake-flask culture and fermentation tank culture on the activated strains to obtain fungus ball seeds; and finally, inoculating the fungus ball seeds into a culture medium, and carrying out mycelium culture, stroma primordium induction and sporocarp development and growth to obtain the sporocarp of the cordyceps sinensis strain AF-Os2007. The obtained cordyceps sinensis strain AF-Os2007 is good in sporocarp commodity character and high in active ingredient content, and has good development and utilization value.