2 results about "Hirsutella sp." patented technology

The application discloses a kind of bat moth larvae double bacteria synergistic infection methods, belong to microbial engineering and medicinal fungus cultivation technical field, comprising: 1-2 age larvae are preprocessed by containing first probiotic feed feeding, optimize intestinal microenvironment;3-4 age larvae are injected and infected using mixed bacterial liquid containing second probiotic and Chinese hairy hair fungus, promote spore germination and colonization;5 age and above larvae are stably cultivated by third probiotic spraying, whole process is matched with dynamic monitoring and threshold emergency control.The technology adopts age differentiation operation of low-age feeding-middle-age accurate injection-high-age spraying, matches each stage functional targeted probiotic, constructs suitable mycelium germination microenvironment by means of probiotic metabolite, inhibits miscellaneous bacteria pollution and provides adenosine synthesis precursor, realizes the excellent effect of infection rate improvement, mortality reduction, adenosine content improvement and effective content improvement, provides efficient feasible technical path for large-scale high-quality cultivation of cordyceps sinensis.

This invention discloses an induction culture medium and a culture method for isolating *Hypericum sinense* strains from *Cordyceps sinensis* branches. The induction culture medium includes sucrose 5-20 g / L, starch 5-20 g / L, potassium nitrate 1-5 g / L, ammonium nitrate 4-8 g / L, zinc sulfate 5-20 mg / L, glycine 1-5 mg / L, calcium chloride 1-5 g / L, magnesium sulfate 1-2 g / L, ascorbic acid 0.3-0.6 g / L, NAA 0.1-2 mg / L, β-cyclodextrin 5-20 g / L, and agar 20 g / L. The culture method includes: (1) generating aerial mycelia from *Cordyceps sinensis* branches; (2) culturing the aerial mycelia in the induction culture medium to obtain *Hypericum sinense* strains. The cultivation method provided by this invention can isolate the Chinese trichomoniasis strain from broken branches of Cordyceps sinensis, which can reduce or avoid resource waste, lower cultivation costs, is convenient and fast, has a high mycelial germination rate, and a fast strain growth rate, which is conducive to the large-scale promotion of artificial cultivation technology of Cordyceps sinensis.