11 results about "Hirsutella sp." patented technology

The invention discloses a method for rejuvenating a hirsutella hepialid strain, which specifically comprises the following steps of: step 1, inoculating a degenerated hirsutella hepialid strain into an extractable stroma culture medium, and culturing until aerial hyphae are generated on the surface of a bacterial colony; 2, performing variable-temperature stimulation on the bacterial colonies grown in the step 1, and producing sporocarp on the bacterial colonies; step 3, taking out the sporocarp in the step 2, performing tissue isolation, inoculating the sporocarp into a PDA culture medium, and culturing until the diameter of a hirsutella hepialid colony is 3cm; and 4, inoculating the hirsutella hepialid strain in the step 3 into a shake flask liquid culture medium with the inoculation amount of about 1cm < 2 > of bacterial colony, filtering and collecting mycelium after culture is completed, and drying and detecting. Compared with a strain before rejuvenation, the strain rejuvenated by the method disclosed by the invention has the advantages that the mycelium yield and the content of active ingredients in bacterial powder are remarkably improved.

The invention discloses a fermentation culture medium and a method for efficiently fermenting hirsutella sinensis, and provides an efficient fermentation culture medium by screening factors influencing the yield of hirsutella sinensis mycelium and the content of medicinal substances (such as adenosine, D-mannitol and the like). The production efficiency of the hirsutella sinensis is remarkably improved (the yield of the mycelium is improved to 18.98 g / L from 7.2 g / L before optimization), and the produced hirsutella sinensis mycelium also has excellent capability of producing active substances (the adenosine content is improved to 1.27 mg / g from 0.82 mg / g, and the D-mannitol content is improved to 115.87 mg / g from 98.78 mg / g). The production process is simple in design, easy to operate and stable in product quality, and is very suitable for being widely applied and popularized in the industrial field due to the characteristics.

The application discloses a kind of bat moth larvae double bacteria synergistic infection methods, belong to microbial engineering and medicinal fungus cultivation technical field, comprising: 1-2 age larvae are preprocessed by containing first probiotic feed feeding, optimize intestinal microenvironment;3-4 age larvae are injected and infected using mixed bacterial liquid containing second probiotic and Chinese hairy hair fungus, promote spore germination and colonization;5 age and above larvae are stably cultivated by third probiotic spraying, whole process is matched with dynamic monitoring and threshold emergency control.The technology adopts age differentiation operation of low-age feeding-middle-age accurate injection-high-age spraying, matches each stage functional targeted probiotic, constructs suitable mycelium germination microenvironment by means of probiotic metabolite, inhibits miscellaneous bacteria pollution and provides adenosine synthesis precursor, realizes the excellent effect of infection rate improvement, mortality reduction, adenosine content improvement and effective content improvement, provides efficient feasible technical path for large-scale high-quality cultivation of cordyceps sinensis.

This invention discloses an induction culture medium and a culture method for isolating *Hypericum sinense* strains from *Cordyceps sinensis* branches. The induction culture medium includes sucrose 5-20 g / L, starch 5-20 g / L, potassium nitrate 1-5 g / L, ammonium nitrate 4-8 g / L, zinc sulfate 5-20 mg / L, glycine 1-5 mg / L, calcium chloride 1-5 g / L, magnesium sulfate 1-2 g / L, ascorbic acid 0.3-0.6 g / L, NAA 0.1-2 mg / L, β-cyclodextrin 5-20 g / L, and agar 20 g / L. The culture method includes: (1) generating aerial mycelia from *Cordyceps sinensis* branches; (2) culturing the aerial mycelia in the induction culture medium to obtain *Hypericum sinense* strains. The cultivation method provided by this invention can isolate the Chinese trichomoniasis strain from broken branches of Cordyceps sinensis, which can reduce or avoid resource waste, lower cultivation costs, is convenient and fast, has a high mycelial germination rate, and a fast strain growth rate, which is conducive to the large-scale promotion of artificial cultivation technology of Cordyceps sinensis.

The invention discloses a membrane separation and extraction method of Hirsutella hepialid mycelium, which comprises the following steps: by taking the Hirsutella hepialid mycelium as a raw material, carrying out pretreatment, enzymolysis and extraction, then carrying out membrane separation, enriching polysaccharide and adenosine, and finally concentrating and drying to obtain the Hirsutella hepialid mycelium extract. According to the method, multiple extraction technologies are combined for use, the extraction efficiency and purity of the hirsutella hepialid mycelium are remarkably improved, the content of polysaccharide and adenosine in the hirsutella hepialid mycelium extract is far higher than that of polysaccharide and adenosine in a traditional water extraction process, and the additional value and market competitiveness of a product are improved.

The invention provides application of hirsutella sinensis polysaccharide in preparation of a neuroprotective agent, and belongs to the technical field of traditional Chinese medicine, and the application comprises the following steps: providing hirsutella sinensis mycelium, extracting the hirsutella sinensis mycelium with water to obtain an extracting solution, concentrating the extracting solution, carrying out alcohol precipitation, and collecting precipitate to obtain crude polysaccharide; purifying the crude polysaccharide through ion exchange chromatography, eluting with sodium chloride with gradient concentration, collecting eluent, concentrating, freeze-drying, carrying out alcohol precipitation, and collecting precipitate to obtain purified hirsutella sinensis polysaccharide; the hirsutella sinensis polysaccharide has an obvious neuroprotective effect on rats suffering from cerebral ischemic stroke caused by middle artery obstruction: the cerebral infarction area is reduced by more than or equal to 35%, the Longa score is reduced by more than or equal to 1.5 score, the bile acid level is improved by more than 4 times, and the content of serum inflammatory factors TNF-alpha, IL-1beta and IL-18 is reduced by more than or equal to 50% in 100mg of middle dose. And a new treatment choice is provided for clinic.

The invention relates to a hirsutella sinensis strain as well as a fermentation method and application thereof, and belongs to the technical field of microorganisms. The strain is separated from a natural cordyceps sinensis sample, and green, high-yield, stable and sustainable Hirsutella sinensis mycelia are obtained through a liquid fermentation process. The strain disclosed by the invention shows excellent growth and proliferation rates in fermentation culture, and the adenosine content of the strain is surprisingly high. In addition, the strain disclosed by the invention shows an excellent bidirectional immunoregulation effect, not only can effectively improve the immune function of macrophages in a resting state, but also has an inhibition effect on excessively activated macrophages.

The invention relates to the technical field of microorganisms, in particular to application and a method for degrading aflatoxin, in particular to application of a hirsutella sinensis strain and paecilomyces hepialid in degrading aflatoxin, and the hirsutella sinensis and the paecilomyces hepialid can effectively degrade various aflatoxin in a short time. The strain has a very good application value in the fields requiring degradation of aflatoxin, especially in the fields of medicines, foods and feeds.

The present application relates to the technical field of polysaccharide extraction, and particularly relates to a Chinese hairy cap polysaccharide, a preparation method and application thereof. Polysaccharides in the Chinese hairy cap are extracted, separated and purified, and four polysaccharide components are obtained. The four polysaccharides can inhibit HIBEC-EMT, avoid liver damage, and can be used for treating and / or preventing primary biliary cholangitis.

The utility model relates to a hirsutella sinensis culture medium preparation device in the technical field of culture medium preparation, which comprises a machine frame, a preparation mechanism and a cleaning mechanism are arranged in a cabinet body of the machine frame, one side of a table top of the machine frame is provided with an object placing table, and the other side of the table top of the machine frame is provided with a split charging mechanism and a conveying mechanism; the preparation mechanism is communicated with the sub-packaging mechanism and is used for conveying prepared culture medium liquid to the sub-packaging mechanism; the conveying mechanism is arranged below the subpackaging mechanism and used for intermittently delivering the cultivation bottles to be filled in a running water mode. According to the hirsutella sinensis culture medium preparation device, the steps of preparation, pH adjustment, boiling, stirring, split charging and the like of hirsutella sinensis culture medium preparation are integrated and processed, and the hirsutella sinensis culture medium preparation device is suitable for batch line production.

The invention discloses a cordyceps sinensis strain AF-Os2007 and a sporocarp cultivation method of the cordyceps sinensis strain AF-Os2007. The cordyceps sinensis strain AF-Os2007 is classified and named as Hirsutella sinensis, the cordyceps sinensis strain AF-Os2007 is preserved in the China General Microbiological Culture Collection Center on October 10, 2025, and the preservation number of the cordyceps sinensis strain AF-Os2007 is CGMCC No.42272. The cordyceps sinensis strain AF-Os2007 has the advantages that the cordyceps sinensis strain AF-Os2007 can be used for preparing the cordyceps sinensis strain AF-Os2007; the sporocarp cultivation method comprises the following steps: inoculating a preserved cordyceps sinensis strain AF-Os2007 into a PDA solid culture medium for activation culture to obtain an activated strain; sequentially carrying out shake-flask culture and fermentation tank culture on the activated strains to obtain fungus ball seeds; and finally, inoculating the fungus ball seeds into a culture medium, and carrying out mycelium culture, stroma primordium induction and sporocarp development and growth to obtain the sporocarp of the cordyceps sinensis strain AF-Os2007. The obtained cordyceps sinensis strain AF-Os2007 is good in sporocarp commodity character and high in active ingredient content, and has good development and utilization value.