135 results about "Bacterial colony" patented technology

The invention discloses lactobacillus plantarum capable of relieving enteritis and toxicity of polystyrene nano-plastics, and the preservation number of the lactobacillus plantarum is CGMCC (China General Microbiological Culture Collection Center) NO.31420. The lactobacillus plantarum can be used for relieving enteritis and toxicity of polystyrene nano-plastics. The strain is obtained by separating and purifying baby feces, and can grow on an MRS culture medium, and the bacterial colony is round, off-white, smooth and opaque. Experiments show that the lactobacillus plantarum can effectively adsorb PS-NPs, promote the PS-NPs to be discharged along with excrement, reduce accumulation of the PS-NPs in organs, and significantly reduce toxicity and inflammatory response of organs such as liver and kidney of mice. In addition, the strain can repair the intestinal barrier function, regulate liver metabolism and relieve enteritis symptoms. The invention further provides a probiotic preparation containing the strain, and the probiotic preparation can be prepared into granules, capsules, tablets or oral liquid and the like and is used for preventing or treating intestinal injury and inflammatory diseases caused by PS-NPs. The strain is safe and efficient, and has a wide application prospect.

The embodiment of the utility model provides mural degerming equipment, which relates to the technical field of cultural relic protection, is used for being matched with a cleaning soft brush for use, and comprises a box body, a vacuum pump, a purification mechanism and a negative pressure pipe. The vacuum pump, the purification mechanism and the negative pressure pipe are all installed in the box body, the negative pressure pipe communicates with the vacuum pump through the box body, and the vacuum pump is used for generating negative pressure in a pipe cavity of the negative pressure pipe. The negative pressure pipe is provided with an adsorption end located outside the box body, and the adsorption end is used for facing the mural body to form a negative pressure area on the mural body. And the purification mechanism is used for purifying gas sucked through the adsorption end. By means of the design of the device, diffusion and propagation of bacterial colonies in the cultural relic cleaning process can be reduced, pollution to the environment is reduced, and the adverse effect on the health of operators during cultural relic maintenance is reduced.

The invention discloses an artificial cultivation method of xylaria fungus sclerotium, and relates to the field of sclerotium cultivation. The method comprises the following steps: collecting xylaria producing sclerotia, obtaining spores by adopting a spore separation method or obtaining core tissues by adopting a tissue separation method, inoculating the spores or the core tissues on an enriched PDA culture medium to be cultured into bacterial colonies, selecting hyphae with vigorous growth vigor at the edges of the bacterial colonies, and performing purification culture until a mother strain only containing a single strain is obtained; performing gene sequencing to identify the strain; propagating the mother strain to obtain an original strain, inoculating the original strain into a sterilized solid culture medium, culturing in a dark place to obtain a cultivated strain, placing the cultivated strain on soil, reserving a space for sclerotium development around the cultivated strain, culturing in the dark place for 33-90 days, and harvesting the sclerotium. The xylaria sclerotium with single germplasm can be cultivated on a large scale, and the problems of wild resource shortage and germplasm mixing are effectively solved.

The invention belongs to the technical field of aquatic product living body transportation, and particularly relates to a soft-shell crab transportation method. The method comprises the following steps: screening and cleaning, softening-keeping treatment, fresh-keeping treatment, microenvironment regulation and treatment after transportation. In the softening treatment, 0.1-3 PSU low-salt seawater containing 0.03%-0.08% of tetrasodium glutamate diacetate is used for soaking / covering, the fresh keeping is ethanol disinfection and 0.05%-0.30% of lemon grass oil emulsified spray, the temperature of the microenvironment is controlled to be 12-18 DEG C, the humidity is 85%-95%, and the oxygen content is 20.90-22.30%. After 72 hours of simulated transportation, the softness-keeping maintenance rate is greater than or equal to 88%, the survival rate is greater than or equal to 98%, the body surface bacterial colony inhibition rate is 91.4%, and the TVB-N value is 15.2 mg / 100g. The method realizes the synergy of softening, fresh keeping and live keeping, is suitable for large-scale transportation, and has high popularization value.

The invention relates to the technical field of detection, in particular to a high-throughput bacterial colony detection sensor based on multi-mode optics and spectrum fusion and a detection method thereof.The method comprises the steps that data of a bright field image, a multi-channel fluorescence image and a near infrared spectrum of a bacterial colony to be detected are collected, and metadata in the collection process is recorded synchronously; preprocessing the data, and extracting multi-dimensional features of bacterial colonies from the preprocessed data; performing fusion processing on the multi-dimensional features in combination with space-time sequence information; identifying and classifying bacterial colonies according to the fused multi-dimensional features; and generating a detection report containing traceability information according to a result after identification and classification. Through multi-modal synchronous data acquisition, targeted data preprocessing, multi-dimensional feature fusion and standardized recognition and classification, high-throughput accurate detection of bacterial colonies is realized, manual intervention is reduced, and reliability and traceability are improved.

The invention discloses an on-line bacterial colony counting device for a disinfection tank. The on-line bacterial colony counting device comprises a counting mechanism, a cover body shell, a control operation table and a culture dish storage mechanism, the cover shell is arranged on the front side of the top of the counting mechanism. The two culture dish storage mechanisms are arranged at the rear ends of the left side and the right side of the outer portion of the counting mechanism correspondingly. The on-line bacterial colony counting device for the disinfection tank realizes full-process automation from sample culture, specified sample taking, transferring and positioning to double-sided counting, greatly reduces the manual intervention intensity, avoids pollution and subjective errors caused by manual operation, improves the counting working efficiency, is suitable for continuous treatment of batch samples, and improves the working efficiency. And through the design that angle adjustment is matched with double-sided synchronous counting and sample rotation covers the whole area, impurity interference is effectively eliminated, counting dead angles are eliminated, and the comprehensiveness and accuracy of a counting result are improved.

The application discloses a chromogenic culture medium for detecting enteropathogenic Escherichia coli, which contains peptone, sodium pyruvate, sodium chloride, D-sorbitol, D-malic acid, bile salt, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, sodium hydroxide, kaolin, bromothymol blue, crystal violet, polyethylene glycol and agar, and the rest is water. The chromogenic culture medium is simple in composition, convenient to prepare and use, and can be used for identifying enteropathogenic Escherichia coli EPEC according to the color of bacterial colonies, and has the advantages of specificity, high efficiency, rapidness, low cost, simple result judgment and the like, and has a wide application prospect in food safety monitoring.

This invention discloses a culture medium for manganese-oxidizing bacteria suitable for low-carbon source environments in water supply networks and its application. The culture medium is prepared by mixing ferric ammonium citrate, manganese sulfate, ammonium sulfate, sodium chloride, magnesium sulfate, dipotassium hydrogen phosphate, and sodium nitrate, followed by high-temperature and high-pressure sterilization to obtain a culture solution. Manganese sulfate is added to the culture solution to obtain the manganese-oxidizing bacteria culture medium. Manganese-oxidizing bacteria are screened using this culture medium. Cast iron pipe scale is subcultured in the medium to enrich the manganese-oxidizing bacteria culture solution. The enriched manganese-oxidizing bacteria culture solution is diluted and spread onto agar solid medium, and incubated at a constant temperature to obtain manganese-oxidizing bacteria colonies. This invention solves the problem of altered bacterial physiological metabolism caused by high concentrations of added manganese ions, effectively improving bacterial activity and manganese oxidation capacity.

The application discloses a kind of multi-modal bacterial colony sample autonomous learning classification identification method and system based on diffusion model, it is related to computer vision technical field, by being input into multi-modal bacterial colony sample classification model to multi-modal bacterial colony sample, obtain classification result;First, the application is by the feature fusion of multi-modal sample input, the correlation between different modalities is in-depth mined, and the semantic information of multi-modal is fully utilized.Secondly, the application combines diffusion model, and the multi-modal bacterial colony sample is classified after being denoised using diffusion model, which is conducive to eliminating inherent noise in multi-modal data samples and interference introduced by human operation errors, and is more conducive to learning.Finally, the application combines autonomous learning, and the model can complete the automatic labeling of new multi-modal samples, can select the most suitable multi-modal sample for training and learning, and continuously improve the robustness of multi-modal bacterial colony sample classification model.

The invention relates to the technical field of tea leaf processing, in particular to a combined sterilization method for tea leaves and application of the combined sterilization method. The invention provides a combined sterilization method of tea leaves. The combined sterilization method comprises the following steps: firstly, carrying out heat sterilization on the tea leaves and then carrying out cold sterilization on the tea leaves, the thermal sterilization method comprises hot air sterilization and / or steam sterilization, and the cold sterilization method comprises high-voltage pulse electric field sterilization. Detection results show that the bacterial colony killing rate and the mould killing rate in the tea leaves can reach 90% or above by adopting the combined sterilization method. In addition, sterilization modes can be flexibly selected for killing different microorganisms according to the types of tea leaves and the requirement for the number of the microorganisms.

The application discloses an intelligent antibacterial hydrogel combined with a photosensitizer and an antibiotic as well as a preparation method and application thereof. The hydrogel takes oxidized dextran 40 as a hydrogel matrix, bonds 5-amino hexyl acetylpropionate into OAHAL through a Schiff base reaction, and is then crosslinked with amikacin to be prepared. The hydrogel has pH sensitivity, can effectively release drugs under an inflammatory slightly acidic environment, realizes on-demand release of the drugs, avoids overuse of the antibiotic, reduces toxic side effects, simultaneously loads two kinds of bactericidal drugs, 5-amino hexyl acetylpropionate and amikacin, and synergistically resists bacteria through two different mechanisms of photodynamic therapy and antibiotics, so that the antibacterial effect is significantly improved, and bacterial colonies caused by various types of wounds can be eliminated, and bacterial infection of a wound surface can be effectively prevented or treated.

An invention disclosing a device and method for the counting and identification of bacterial, mold or yeast colonies based on hyperspectral image capture and processing. A device for acquisition of hyperspectral images comprising camera capable of hyperspectral imaging, a hole inspection lens array and a light source arranged in or around a light housing is described. A method comprising a deep learning algorithm is made available for the processing of acquired hyperspectral data in order to detect, identify and count bacterial, mold or yeast samples contained within a Petri dish. In particular, the use of a hole inspection lens array allows acquisition of hyperspectral images of the complete inner surface of a Petri dish which, combined with the use of a deep learning algorithm ensure a high level of accuracy and sampling frequency.

The invention discloses a green biological soil bedding capable of improving successive cropping obstacles and a preparation method, application and application method.The green biological soil bedding comprises a fiber net layer and a PHA composite layer, the fiber net layer takes plant fiber raw materials as main base materials, the rigidity and supporting force of a mulching film are improved, and therefore the mulching film can be applied to the field of agricultural planting. Collapse deformation caused by gravity or external force in soil is avoided, and the overall stability is enhanced; the PHA composite layer and the fiber net layer have the degradable characteristic, so that after the bedding is used, a carbon source can be continuously provided for beneficial microorganisms in the components, breeding and colonization of the beneficial microorganisms are promoted, convenient conditions are provided for activity and breeding of target bacterial colonies, and in the cooperative work process, growth of pathogenic bacteria can be inhibited, so that the soil micro-ecology is improved, and the soil quality is improved. Continuous cropping obstacles are improved, the yield is increased, various problems caused by traditional mulching film residues are avoided, and green and sustainable development of agriculture is promoted.

The invention discloses staphylococcus albus and application thereof in fermentation of high-salt liquid-state clean label soy sauce. Belongs to the technical field of food fermentation. The staphylococcus fish fermenti CS1.22 is separated from soy sauce mash for the first time, and an application example of the staphylococcus fish fermenti CS1.22 in high-salt liquid-state clean label soy sauce is not reported yet at present. The strain can effectively inhibit bacillus causing deterioration of soy sauce, the bacteriostatic effect of the strain is mainly non-acid bacteriostasis, the bacteriostatic action pH range is 4.0-5.0, the strain is suitable for being applied to soy sauce fermentation, and the total number of bacterial colonies of finished soy sauce can be effectively reduced; when the high-salt liquid-state soy sauce is added to the fermentation of the high-salt liquid-state soy sauce, the content of volatile flavor substances can be remarkably increased while the content of amino acid nitrogen is increased, so that the high-salt liquid-state'clean label 'soy sauce is rich in flavor and excellent in quality.

The invention relates to the field of food sterilization, and particularly discloses an ultrahigh-temperature instant sterilization process and system for solids. The solid matter ultra-high-temperature instantaneous sterilization process comprises the step of putting solid matter into a sterilization space, and the sterilization process comprises the following steps: S1, injecting overheated hot water into the sterilization space to carry out high-temperature instantaneous sterilization on the solid matter; s2, discharging overheated hot water; and S3, the solid matter is placed in a cooling space, and rapid cooling is achieved. High-temperature instantaneous sterilization is carried out on the solid matter through overheated hot water, the solid matter is immediately placed in the cooling space after sterilization, rapid cooling is achieved, the overall temperature curve of the solid matter is in an n shape in the process, through the process from instantaneous high-temperature heating to rapid cooling, spores, bacterial colonies and escherichia coli are effectively killed, efficient sterilization is achieved, and the solid matter can be rapidly cooled. Due to instantaneous heating and rapid cooling, in the process of the solid matter, the surface temperature changes rapidly, but the internal temperature changes slowly, the high temperature time is short, and the quality and flavor of the solid matter are not affected.

The utility model provides a kind of bacterial counting device and water treatment system, it is related to the technical field of water quality microbiological detection device, sample pretreatment module includes filter assembly, and the water inlet end of filter assembly receives inlet water sample through pipeline, and the outlet end of filter assembly is connected with dyeing bin through pipeline, and pipeline intercommunication has drain pipe between filter assembly and dyeing bin, for the redundant water sample of outer discharge;CCD imaging module includes the imaging platform of pipeline intercommunication with the output end of dyeing bin, and automatic focusing assembly and fluorescence microscope are arranged on the upper side of the central axis of imaging platform, for showing the bacterial colony of fluorescent dye dyeing, and fluorescence microscope is electrically connected with CCD camera;CCD camera is electrically connected with image processing module, for outputting bacterial concentration and statistical distribution diagram, the technical problem of increasing culture time consumption and high detection cost of artificial operation in prior art, reaches fast detection, cost low and high accuracy technical effect.

The invention relates to a bacterial colony sampling module, a sampling method and an experiment system, and is applied to the field of microorganism experiment automation equipment. The bacterial colony sampling module comprises a fixing frame, a rotary driving assembly, a plurality of sampling needle units and a station driving assembly, and the fixing frame comprises a plurality of functional stations; the rotary driving assembly is rotationally arranged on the fixing frame; the sampling needle units are connected to the rotary driving assembly, are arranged at intervals in the circumferential direction of the rotary driving assembly, and can synchronously rotate along with the rotary driving assembly so as to sequentially pass through each functional station; the station driving assembly is installed on the fixing frame, corresponds to the function stations and is used for driving the sampling needle unit to execute operation corresponding to the function stations when the sampling needle unit rotates to the corresponding function stations. The multiple sampling needle units are driven by the rotatable driving assembly to sequentially flow through all the functional stations, so that the multiple functional stations can be utilized at the same time, different sampling needle units can perform different procedures at the same time, and the overall efficiency of bacterial colony treatment is improved.

This utility model discloses an automated sampling robot for dust and bacterial colony detection, belonging to the field of automated equipment for environmental monitoring and biological experiments. It includes a cart, a rotating gimbal, a support frame, double support plates, a double limiting structure, and a mechanical gripper clamping system. The first limiting structure uses an arc-shaped plate to press against the box, while the second limiting structure uses an air cushion and a top block to fix the culture dish. The mechanical gripper replaces suction cup gripping, improving stability. The double support plates respectively carry the culture dishes for bacterial and dust detection, and the rotating gimbal allows for switching between workstations, enabling dual sampling of dust and bacteria. The rotation angle of the support plates dynamically adjusts according to the number of culture dishes, supporting fixed-point interval detection. This utility model is adaptable to culture dishes of different sizes, has a high degree of automation, and can precisely capture the dynamics of bacterial and dust colonies, providing data support for multi-dimensional environmental analysis.

The invention relates to the technical field of yeast fermentation, and discloses a yeast culture tank convenient for transplanting bacterial colonies, the yeast culture tank comprises a fermentation tank, the outer wall of the fermentation tank is provided with a sampling mechanism, the outer wall of the fermentation tank is provided with a mixing mechanism, the bottom of the fermentation tank is provided with a discharge valve, and the bottom of the fermentation tank is fixedly connected with supporting legs. The sliding teeth are slidably connected to the inner wall of the top of the fermentation tank, the pulling L-shaped rods are fixedly connected to the outer walls of the sliding teeth, the vertical long pieces drive the first reciprocating lead screws to rotate in the rotating process, the first reciprocating lead screws are in meshing transmission with the gear ring through the bevel gear set in the rotating process, and therefore autorotation is generated; in the rotating process of a reciprocating screw rod I, a vertical telescopic rod is driven by a crossed spiral groove in the circumferential surface to do reciprocating motion under the limiting of a limiting inclined sliding groove, so that yeast transported in the sampling pipe is stirred and dredged, and the yeast is prevented from being blocked in the sampling process.

The invention discloses a pseudomonas aeruginosa (Pseudomonas aeruginosa) T-5 with the preservation number of CCTCC (China Center for Type Culture Collection) NO: M 20251946, a microbial agent of the pseudomonas aeruginosa (Pseudomonas aeruginosa) T-5, and an application of the pseudomonas aeruginosa (Pseudomonas aeruginosa) T-5. The strain is gram-negative bacteria, cells are rod-shaped, bacterial colonies are large, the center of the strain protrudes and is milky white, and blue-green or tawny pigments can be generated; when the strain is cultured in an LB liquid culture medium under the conditions of 30 DEG C and 160 rpm, the strain enters a logarithmic phase in 2-4 h and enters a stable phase in 24 h, the OD600 value in the stable phase reaches 1.6 + / -0.1, and the viable count of fermentation liquor in 24 h is larger than or equal to 10 < 10 > CFU / mL; and the method is suitable for repairing a severe environment with composite organic pollution.

The invention relates to the technical field of food processing, in particular to frozen camel milk with milk skin and a preparation method thereof.The preparation method comprises the following steps that firstly, after camels produce milk, the temperature of the raw material camel milk is reduced to 0-2 DEG C from 37 DEG C within 10 minutes, the raw material camel milk is stored in 304 stainless steel, and nitrogen is introduced into a tank for protection during storage; step 2, after the raw milk reaches a factory, the total number of bacterial colonies is less than or equal to 20000 CFU / g, the protein content is more than or equal to 3.8 g / 100g, and the fat content is more than or equal to 4.5 g / 100g; through a series of measures such as rapid cooling, low-temperature storage, nitrogen introduction for protection, production and processing time shortening, low-temperature centrifugal separation, appropriate-temperature mixing and stirring, rapid cooling and the like, microbial reproduction is effectively inhibited, the oxidation speed of fat and protein in camel milk is slowed down, and decomposition and loss of nutritional ingredients are reduced; active nutrient substances in the camel milk are reserved to the maximum extent, and meanwhile, the content of active lactoferrin in the stock solution is increased.

The invention relates to the technical field of saccharomyces cerevisiae expression foreign protein, and discloses a saccharomyces cerevisiae strain with ASFV p14.5 displayed on the surface and a construction method and application thereof, and the method comprises the following steps: (1) synthesizing a delta site homologous arm sequence; (2) seamlessly connecting the homologous arm sequence of the delta site to a carrier pET23a, connecting a selective marker tryptophan biosynthetic gene TRP1 to the 5'end of the upstream homologous arm sequence of the delta site, and sequentially connecting an Aga2 gene, an E120R gene, a T2A peptide gene and an mCherry gene to the 5 'end; (3) carrying out enzyme digestion on two sides of the delta site through restriction endonuclease salI, and recovering a linearized fragment through agarose gel; and (4) integrating the recovered fragments into a host saccharomyces cerevisiae genome through a chemical conversion method, and culturing until bacterial colonies grow. The surface display type saccharomyces cerevisiae strain obtained by the invention not only has the stability of integrative saccharomyces cerevisiae for expressing foreign protein, but also can be used for more easily and more intuitively screening a high-expression quantity saccharomyces cerevisiae strain by combining the T2A peptide with mCherry.

The invention relates to talaromyces pseudomonas hzt-3'as well as a preparation method and application of the talaromyces pseudomonas hzt-3 ', and belongs to the technical field of application of microorganisms. The strain is preserved in the China Center for Type Culture Collection, the preservation number of the strain is CCTCC NO: M 20251797, and the strain is classified and named as (Talaromyces pseudomonas hzt-3 '). The method comprises the following steps: collecting moss and vegetation soil samples from basalt, diabase and dolomite; diluting bacterial colonies in the sample, and separating and purifying the bacterial strains by using a Czapek culture medium; anorthite and serpentine are used as inorganic salt sources for strain screening, and strains which grow stably and have a good leaching effect on calcium magnesium silicate minerals are obtained; the strain is applied to efficient leaching of anorthite, serpentine and wollastonite, and compared with a sterile control group, the effect of improving the leaching rate of calcium and magnesium ions is remarkable. The method solves the problem that the reaction rate of mineral sequestration carbon dioxide is slow due to the fact that ions in natural silicate minerals are difficult to leach, and has great significance.

The invention relates to the technical field of veterinary diagnosis in intelligent agriculture, in particular to a dairy cow mastitis pathogen rapid identification and medication guidance system, which comprises the following modules: an image acquisition module, which is configured in a microbial culture device and comprises a high-resolution image sensor and a multispectral illumination unit, the acquisition module is used for acquiring digital images of culture dishes containing pathogenic bacterium colonies and drug sensitive paper sheets; and the edge calculation module is in communication connection with the image acquisition module and is used for carrying out noise filtering and contrast enhancement processing on the acquired petri dish digital image and extracting key image area data including a bacterial colony area and a drug sensitive paper area. According to the invention, through multi-module cooperation and data closed loop, rapid identification and accurate medication of pathogenic bacteria are realized, so that the problems of treatment delay and drug resistance development caused by dependence on artificial culture and empirical judgment and long diagnosis period in traditional methods are improved.

The invention discloses a preparation method of a recombinant antibacterial peptide Hc-CATH and application of the recombinant antibacterial peptide Hc-CATH in blueberry preservation, and the preparation method comprises the following steps: synthesizing an Hc-CATH target gene as shown in SEQ ID NO: 2, and connecting to a pPICZ alpha A carrier to form a recombinant plasmid pPICZ alpha A-Hc-CATH; the method comprises the following steps: carrying out linear treatment on a recombinant plasmid pPICZ alpha A-Hc-CATH by using Sac I restriction enzyme, and transferring the treated plasmid into pichia pastoris to obtain a recombinant strain with a target gene; performing inducible expression on the screened positive transformant by using methanol, and collecting the fermented supernate to obtain the high-concentration antibacterial peptide; wherein the original amino acid sequence of the antibacterial peptide is as shown in SEQ ID NO: 1. The recombinant Hc-CATH antibacterial peptide reduces the number of blueberry bacterial colonies, weightlessness and anthocyanin loss, and weakens the respiration intensity of blueberries.

The invention provides an automatic bacterial colony circulation detection system and a culture dish circulation method.The automatic bacterial colony circulation detection system comprises a lifting device, a multi-platform conveying device, a descending device and a visual detection conveying table which are connected in a closed loop mode, the lifting device or the descending device comprises a frame, and a plurality of outer ring chain wheels and a plurality of inner ring chain wheels are hinged to the frame through transmission shafts; the outer ring chain wheels are sleeved with outer chains to form tooth meshing connection, the inner ring chain wheels are sleeved with inner chains to form tooth meshing connection, and any outer ring chain wheel or any inner ring chain wheel is in driving connection through a lifting motor. A material carrying table with bending flexibility is arranged between the outer chain and the inner chain, the two ends of one side edge of the material carrying table are hinged to the inner chain through short lug plates, the two ends of the other side edge of the material carrying table are hinged to the outer chain through long lug plates, and the material carrying table is connected with a multi-platform conveying device at the top ends of the outer chain and the inner chain. The material carrying table is connected with a visual detection conveying table at the bottom ends of the outer chain and the inner chain.

The invention discloses a method for evaluating self-cleaning and degerming functions of a washing machine, which comprises the following steps: (1) preparing microbial pollutants and carriers thereof: selecting an aluminum sheet, and uniformly coating the surface of the aluminum sheet with the microbial pollutants to obtain a microbial pollutant carrier; the microbial pollutant carriers are fixed to the center of the bottom of an inner barrel of the washing machine and evenly fixed to the inner wall of the inner barrel at equal intervals, and at least four microbial pollutant carriers are fixed to the inner wall of the inner barrel; starting a self-cleaning program, recovering each microbial pollutant carrier after the self-cleaning program is operated, and measuring the number of bacterial colonies; (3) positive control group test: immobilizing a microbial pollutant carrier in the same way as that of the test group; starting a conventional washing program except for the self-cleaning program, and after the conventional washing program is operated, recycling the microbial pollutant carrier according to a test group method for determining the colony count; and (4) calculating the self-cleaning degerming rate.

The utility model relates to the technical field of microbial culture, in particular to an inoculating needle for quantitatively collecting bacterial colonies, which comprises a sampling needle and a scraping tube, a groove is arranged inside one end of the sampling needle, an opening is arranged at one end of the sampling needle close to the groove and communicated with the groove, a connecting port is arranged on the side wall of one end of the sampling needle close to the groove, and the scraping tube is communicated with the opening. The connecting port is communicated with the groove, the inner wall of the connecting port, the inner wall of the groove and the end part of one end, close to the groove, of the sampling needle are all provided with anti-skid holes, the outer side wall of the sampling needle is sleeved with the scraping pipe, and the inner wall of the scraping pipe is in sliding connection with the outer side wall of the sampling needle, so that automation is easier to realize in the operation process; and the working efficiency and accuracy of a laboratory can be improved.

The present disclosure provides a water purifier and a bubble flushing device thereof, the water purifier comprising a water tank, the bubble flushing device comprising: a gas suction pump for sucking gas from a gas storage module and inputting into a water-gas mixing module; a first pressure detection module for detecting a first pressure value of the gas output by the gas storage module; a second pressure detection module for detecting a second pressure value of the gas input into the water-gas mixing module; a control unit for adjusting a current opening degree of the gas storage module according to the first pressure value, so that the second pressure value is within a preset range; and the water-gas mixing module for mixing the input gas with water, forming bubble water and inputting into a bubble water inlet to flush the water tank. The present disclosure stabilizes the pressure value of the bubble water input into the water tank of the water purifier by arranging two pressure detection modules, and can further flush the water tank to kill the bacterial colonies in the water tank, thereby ensuring the safety and health of the user's water, protecting the user's health and improving the user's experience.