1781 results about "Bacterial colony" patented technology

The present invention is directed to novel compositions and methods utilizing nanoparticles comprising protein cages for delivery of imaging and antimicrobial agents to biofilm forming bacterial colonies.

The invention relates to enterococcus faecium for feeding as well as a freeze-drying fungicide and applications thereof, and discloses enterococcus faecium LAB12 CGMCC (China General Microbiological Culture Collection Center) No.4847 which is grampositive cocci, has no spores, grows well on an MRS agar plate, forms a round bacterial colony with the diameter of 0.5-1mm within 48 hours and is used for feeding, and the bacterial colony is round, smooth and upheaved, and is a shape of grey white dewdrop; the enterococcus faecium grows in a facultative anaerobic condition; the growth temperature range is 10 DEG C-45 DEG C; the optimum growth temperature is 30 DEG C-40 DEG C; and the growth pH value is 4-10, and the optimum pH value is 6.0. The freeze-drying fungicide formed by the bacterial strain is nontoxic and harmless, is gastric juice resistant, is cholate resistant, has a high inhibitory effect for multiple harmful bacteria, has a long quality guarantee period, can be widely applied to birds and livestock breeding to strengthen the animal disease-resistant capability, and is expected to be the substitution of antibiotics for feeding.

The invention discloses a method for producing a complex micro-ecological preparation with microbial agents and enzyme. According to the action principles and characteristics of every microbial strain, the method adopts scientific combination and adds enzyme preparations and acidifier to enable microorganisms in the product to grow rapidly and take dominance in fermented feed and animal bodies so as to reduce the growth opportunity of miscellaneous bacteria. The preparation comprises the following components: bacillus subtilis, lactobacillus plantarum, candida utilis yeast, lactobacillus acidophilus, protease, amylase, cellulase, xylanase, mannanase, galactosidase, pectinase and citric acid. As all strains of the product adopt liquid deep fermentation culture, the non-polluting property of the product is guaranteed. Liquid microbial agents ensure that the number of bacterial colonies of the product is more than 100 million and the product is free from the pollution of other miscellaneous bacteria, while solid microbial agents ensure that the number of bacterial colonies of the product is more than 1 billion and the proportion of miscellaneous bacteria to effective bacteria is not more than 0.0001. The preparation contains bacillus, lactic acid bacteria and yeast, can apply to the processes of making green-yellow storage feed, fermenting feed, soaking feed, and the like, can be directly added to feed for feeding, and can improve the growth performance of ruminant animals, poultry, pigs, aquatic products and special animals.

The invention provides a biologically-fermented complete compound feed. In the complete compound feed, a protein feed and an energy feed are fermented respectively and are matched with a premix compound, or the protein feed and the energy feed are matched together to be fermented, and are matched with the premix compound, or the protein feed, the energy feed and the premix compound are matched together to be fermented, wherein the premix compound accounts for 2 to 6 percent of the total weight of the feed; and the fermentation is performed by adding feed-level microbes which are beneficial tothe survival or growth period of raised animals into the feed or adding feed-level enzymic preparations into the feed, wherein the fermentation rate is between 50 and 100 percent, and the fermented feed contains a large number of fermentation products and beneficial bacterial colonies. The complete compound feed has the advantages of high utilization rate of absorption, growth promotion, disease prevention, disease resistance and high cost performance and is easy to digest, and safe, so the feed industry and the cultivation industry have new development space.

The invention relates pseudomonas, use thereof and a method for removing cadmium pollution to the environment. The pseudomonas class is named pseudomonas putidaHN103 and the collection number in a collection center is CCTCCNo.M2011184; and the shape and characteristics of the pseudomonas putidaHN103 comprise: 1) on a culture medium, a bacterial colony which used to be colorless and transparent turns white, smooth and convex-like with irregular edges; and 2) according to the result of bacterial microscopic examination, pseudomonas belongs to brevibacterium and is gram negative. The use of the bacteria is the use of the bacteria as a biological repair material for removing cadmium pollution. The method for removing cadmium pollution to the environment comprises the following steps: 1) regulating the cadmium ion concentration in sewage to a range in which pseudomonas can grow normally; 2) placing pseudomonas; 3) keeping the pseudomonas in the sewage for a certain time period; and 4) removing bacterial absorbing cadmium ions. The pseudomonas requires small investment, is low in cost, high in adaptability and capable of effectively removing cadmium, can quickly and obviously lower the concentration of cadmium ions in the environment and is suitable for biological remediation environments polluted by cadmium.

The invention relates to a bio-organic fertilizer and a method for preparing the bio-organic fertilizer which prevents and treatments soil insects. The bio-organic fertilizer is added with active bacteria and other nutrition bacterial genus, wherein the active bacteria and other nutrition bacterial genuses can prevent and treat soil insects. The bio-organic fertilizer comprises biological agents and organic fertilizers, wherein the microbial agents comprise bacterial colonies of Japanese beetle bacilli, withered grass bacilli, enormous bacilli, jelly-formed bacilli, brown ball nitrogen-fixing bacteria and bacillus licheniformis. The Japanese beetle bacilli are the main type of bacterium which prevents and treats soil insects and other types of bacteria are mainly used for fixing nitrogen, decomposing phosphorus, decomposing potassium, and preventing and treating soil-borne diseases. The organic fertilizer comprises livestock and poultry manure and edible mushroom dregs. The livestock and poultry manure and the edible mushroom dregs are mixed, ferment and are thoroughly decomposed, the microbial agents are added and the bio-organic fertilizer is prepared. The bio-organic fertilizer and the method for preparing the bio-organic fertilizer which prevents and treatments soil insects can effectively kill larvas of soil insects. Bacterial colonies of microorganisms grow by fixing nitrogen, decomposing phosphorus and decomposing potassium, the structure of soil is improved, the organic content of soil is improved, the quality of crops is improved and the crop yield is improved.

The invention provides bacillus amyloliquefaciens SZ-60 which is characterized in that the collection number is CGMCC No.8277. The bacterial colony formed by the strain on a beef-extract peptone (NB) medium by virtue of single-cell reproduction is irregular with oyster white color, slight upheaval at the center and wet and semitransparent surface; the microscopy is of a rod shape, and the size is (0.3-0.4)*(3.2-3.3)microns; with flagella, G- and spores, the strain can grow on the beef-extract peptone (NB) medium containing 2-5% of NaCl; the formula of the beef-extract peptone (NB) medium contains 3.0g of beef extract, 10.0g of peptone, 5.0g of NaCl, 17g of agar and 1,000ml of water, and the pH is 6.8-7.2. The bacillus amyloliquefaciens realizes a remarkable antagonistic action on the main pathogenic bacteria causing ginseng root rot, epidemic diseases, sclerotinia sclerotiorum, cylindrocarpon destructans, black spot and damping off. The invention also provides an application of the bacillus amyloliquefaciens SZ-60 in preventing plant fungal diseases, or application in preparing a microbial preparation for preventing plant fungal diseases.

The invention provides a cadmium pollution bioremediation agent which is composed of the following ingredients in parts by weight: 10-20 parts of core rhzomorph, 10-20 parts of a nutritional ingredient, 2-6 parts of an enzyme activity substance and 54-78 parts of an rhzomorph carrier. The invention further provides an application of the cadmium pollution bioremediation agent. The cadmium pollution bioremediation agent is reasonable in microorganism specie collocation and high in comprehensive function. Microorganisms in the core rhzomorph can be planted and grow in soil rapidly to form strong bacterial colonies, and heavy metal ions, namely, cadmium Cd<+1> can be directly absorbed to microbial cell walls or into cells to be cured and passivated. Besides, bacillus cereus and beer yeast in the composition have high absorption and passivation function on heavy metal ions, namely, cadmium Cd<+1>, chromium Cr<+1> and lead Pb<+1>, due to the high comprehensive function, the mobility of the heavy metal ions, namely, cadmium Cd<+1>, is reduced, and the purpose of reducing absorption of crops on the heavy metal ions is achieved.

The Bacillus amyloliquefaciens HRH 317 strain separated and screened from soil is preserved on March 15th, 2013, and the preservation number is CGMCC No. 7314. The strain is cultured in beef extract-peptone medium, The thalli form a large number of spores, the thalli are rod-shaped before the spores are formed, are arranged individually or in a chain, and are Gram-positive. After cluture for 3 days, the bacterial colonies are milk-white with a diameter of about 6.75mm and with wrinkles on the surfaces, have non-transparent optical characteristic, has projections and are rounded, and the edges are undulant or petaloid. During liquid standing culture, mycoderm is generated. After homology comparison with the 16SrDNA gene sequence of known strains, the homology with Bacillus amyloliquefaciens reaches 99%-100%. The phylogenetic tree of the 16 SrDNA sequence shows that the strain has the highest similarity with Bacillus amyloliquefaciens strain BGP20. After physiological and biochemical identification, the strain is identified as Bacillus amyloliquefaciens.

When multiple kinds of bacterial colonies are present in a petri dish and, for example, a drug tolerance is to be measured, harvesting of mixed colonies of different types of bacteria makes it impossible to accurately determine the drug tolerance. Also, it is required to improve the throughput of a device for harvesting a bacterial colony. From images illuminated from multiple directions, isolating bacterial colonies are automatically extracted. Next, the image feature amounts are calculated from the multiple images that are illuminated from multiple directions and colonies are grouped depending on the feature amounts. Then, bacterial colonies to be harvested are determined based on the results of the grouping.

The bacterial colony number sample for verifying food microbiological capacity relates to quality control technology in microbiological detection. The sample is matrix with added Bacillus cereus as leading bacteria and background bacteria. The matrix is practical food, and the background bacteria include one or several kinds of serratia marcescens, Citrobacter freundii, enteroaerogen and escherichia coli. The sample of the present invention has homogeneous colony number content, high finishing rate, and high stability, and may be used in test capacity verification of domestic and international labs. In the same time, the capacity verifying sample may be used in verification of detection method, calibration of test instrument, quality control and check of test result and other aspects.

The invention mainly provides a bus stop site selection and layout optimization method based on passenger trip spatial distribution. The bus stop site selection and layout optimization method based on the passenger trip spatial distribution mainly comprises construction of a bus stop site selection optimization model and a solution algorithm thereof. The bus stop site selection optimization model takes minimization of walking distance of all the residents for taking buses as a goal and considers realistic constraint factors such as maximum walking distance between a resident dense point and a bus stop and upper and lower bounds of distance between adjacent stops, the solution algorithm of the bus stop site selection optimization model is a novel hybrid algorithm and combines advantages of a bacterial foraging optimization algorithm and a group random search algorithm, and according to problem characteristics, individual bacterium coding, initial bacterial colony generating, individual bacterium evaluation function generating and bacterial foraging operations are redesigned. The bus stop site selection and layout optimization method based on the passenger trip spatial distribution scientifically and reasonably determines bus stop positions by combining a real road topological structure according to trip spatial distribution characteristics of residents nearby a route, so that resident trips are facilitated, and bus operation efficiency is also improved.

The invention discloses bacillus velezensis HNt7 with broad-spectrum antibacterial activity as well as a preparation method and application. The antagonistic bacterium bacillus velezensis HNt7 has a number of CCTCC (China Center for Type Culture Collection) M 2018022. The preparation method comprises the following steps: A, selecting a bacillus velezensis HNt7 strain individual bacterial colony under a sterile condition and inoculating into a culture medium; adjusting the temperature and carrying out shake culture; B, filling a fermenting solution into each bottle and adding individual bacterial colony bacterium liquid according to the ratio; putting the bottle into a large-amplitude constant-temprature freezing shaking table and carrying out the shake culture; C, separating the antagonistic bacterium: immersing with alcohol with concentration and rinsing with sterile water; carrying out dark culture in a culture box and transferring a grown bacterial colony into a new culture medium and carrying out lineation separation and purification on the bacterial colony; D, after separating and purifying, obtaining the bacillus velezensis HNt7. The bacterium is used for preparing a medicinefor treating or preventing pear fruit rot disease. The strain has a remarkable disease-prevention effect and the prevention effect reaches 70.76 percent or more. After the bacillus velezensis HNt7 isapplied to pear trees, advanced defoliation of the pear trees is remarkably inhibited and the tree vigor is easy to enhance.

The invention relates to the field of banana cultivation, in particular to a compound microbial agent for preventing and treating a banana wilt disease and a prevention and treatment method. The compound microbial agent comprises paenibacillus polymyxa, bacillus subtilis, bacillus thuringiensis, pseudomonas stutzeri, bacillus amyloliquefaciens, cold-resistant brevibacteriaceae, trichoderma and metarrhizium anisopliae. By means of a reasonable proportion, all strains are mutually influenced and promoted, and the prevention and treatment effect on the banana wilt disease is greatly improved. The prevention and treatment method of the banana wilt disease can effectively adjust the composition of bacterial colonies in soil, promote growth and propagation of probiotics and antagonistic bacteria in the soil, rapidly restore and stabilize the balance of the bacterial colonies in the soil, prevent and treat occurrence and spread of the banana wilt disease, effectively decrease the occurrence rate of the banana wilt disease and maintain the stable and high yield of bananas.

The invention discloses porcine O-type foot-and-mouth disease virus recombinant baculovirus as well as a preparation method and application thereof. Sequences of VP0, VP1 and VP3 genes are artificially synthesized by referring to an FMDV (Foot And Mouth Disease Virus) O-type epidemic strain gene sequence; the VP0, VP1 and VP3 genes are connected to pFBDPHmHNM1P10eGFP plasmid by adopting the plasmid as a framework vector, so that a baculovirus transfer vector pFBDPHmVP013 is obtained. The baculovirus transfer vector pFBDPHmVP013 is mixed with DH10Bac escherichia coli competent cells, and the positive bacterial colony is selected to obtain a recombinant shuttle vector Bacmid; the shuttle vetcor Bacmid is transferred with a sf9 cell, and the recombinant baculovirus QP-Ac-FVLP is obtained by collecting the cell supernatant. The recombinant baculovirus can be used for efficiently expressing FMDVVP0, Vp1 and Vp3 proteins and forming virus-like particles. And the virus-like particles are used for preparing subunit vaccine, so that the organism is induced to generate specific immunity response after the mouse is immunized.

A system and a method of detecting and characterizing a bacterial colony are presented in which the results are determined within about 48 hours. The bacterial colony is disposed on a substrate and placed between a laser and detector. Light from the laser impinges upon and is scattered by the bacterial colony. The forward scattered light is detected by an optical detector. The signal from the optical detector is analyzed by an analyzer and displayed or supplied to a storage medium for review. As different strains of bacteria possess unique forward scattering fingerprints, the particular strain may be identified.

The invention discloses a method for preparing mead with a fruit flavor. In the method, honey is used as a raw material, and the mead is prepared by two times of fermentation of flavor-producing yeasts, namely primary fermentation for producing wine and secondary fermentation for generating flavor. The alcohol volume of the mead prepared by the method is 6 to 6.5 percent and the reducing sugar concentration in the mead is 60 to 65g/L. The mead does not contain cane sugar, artificial essence, pigment and preservative. The mead tastes pure, slightly sweet and fresh and does not taste bitter. The mead has a pure banana flavor and wine fragrance and contains 17 amino acids and various vitamins. The mead is pure mead brewed wine with a fruit flavor. The result of a test performed on the product prepared by the method according to B2758-2005 brewed wine sanitation standards shows that the mead meets the requirements of national brewed wine sanitation standards in total sulfur dioxide (SO2) content, lead content, total number of bacterial colonies, number of coliform colonies, number of enteropathogenic bacteria and the like. The mead can be drunk and satisfies the demands of people for low-alcohol-volume nutritive wine.

The invention discloses a composite microbial deodorant and a preparation method thereof. Each liter of the deodorant comprises the following bacteria: photosynthetic bacterium with a viable count of 1.3-7.5*108 CFU, bacillus with a viable count of 0.9-7.8*108 CFU, saccharomycete with a viable count of 1.02-1.92*108 CFU, nitro-bacterium with a viable count of 1-10*108 CFU, thiobacillus with a viable count of 1-6*108 CFU, melanomyces with a viable count of 4.8-13.5*108 CFU, and aspergillus flavus with a viable count of 4.8-11.7*108 CFU. The bacteria mentioned above are basically obtained from garbage, and domesticated by garbage leachate. The deodorant can act on the garbage and garbage leachate, and thus cut off the odor source. The beneficial bacterial colonies can decompose and degrade odorous pollutants in garbage, eliminate the garbage odors, and generate a great amount of beneficial metabolic products and enzymes.

The invention relates to a method for producing compound feed from mixed strains through united fermentation. According to the method, corn bran is taken as a main raw material and is subjected to batch culture through Aspergillus niger, trichoderma, bacillus subtilis, saccharomycetes and lactic acid bacteria, the microbiological feed is produced through combined conversion and solid fermentation, and the method comprises technological steps as follows: pretreatment of the raw materials, compounding of a mould starter, blending of yeast composite fermentation preparation, preparation of lactic acid bacteria starter, separate solid fermentation, drying, smashing and the like. According to the invention, the corn bran resource can be utilized sufficiently and efficiently, and the additional value of the corn bran resource is increased; harmful bacterial colonies in intestinal tracts can be reduced, the functions of conditioning healthcare, increasing the feed utilization rate, preventing diseases, promoting growth, reducing usage of drugs such as antibiotics and the like and improving the quality of livestock and poultry products are effectively performed, excrement stink is reduced, the breeding environment is improved, and one high-quality and low-cost green biological feed for feeding livestock and poultry is provided.

The present invention provides Bacillus vallismortis SZ-4, which has the preservation number of CGMCC No.8273. According to the present invention, the strain grows on a beef extract-peptone (NB) culture medium in a single cell reproduction growth manner to form bacterial colonies, and the bacterial colonies have characteristics of round shape, milk white color, slightly elevated center, and moist and transparent surface; microscopic examination results show that the Bacillus vallismortis SZ-4 has a rod shape, has flagella, has a size of 0.7-0.82*1.6-2.2 mum, presents the positive Gram staining effect, has spores, and can grow on a beef extract-peptone (NB) culture medium containing 2-5% of NaCl, wherein the beef extract-peptone (NB) culture medium comprises: 3.0 g of a beef extract, 10.0 g of peptone, 5.0 g of NaCl, 17 g of agar and 1000 ml of water, and the pH value is 6.8-7.2; and the Bacillus vallismortis SZ-4 provides significant antagonism effects for pathogenic bacteria causing ginseng root rot, blight, sclerotinia sclerotiorum and botrytis cinerea. The present invention further provides an application of the Bacillus vallismortis SZ-4 in prevention and control of plant fungal diseases or preparation of microorganism preparations for prevention and control of plant fungal diseases.

The invention discloses a polysporus trichoderma (Hypocrea pachybasioide) FSR-97 strain, wherein the preservation unit is China General Microbiological Culture Collection Center, the preservation date is 19, September, 2014 and the preservation number is CGMCC No.9691. The strain is cultured on a potato dextrose agar culture medium (PDA) plate at 25 DEG C and rapidly grows, and a monospore is germinated to form a white bacterial colony; the colour on the back surface of the bacterial colony is transited from colourless to yellow. The polysporus trichoderma (Hypocrea pachybasioide) FSR-97 has an effective broad-spectrum antibacterial effect on the main pathogenic bacteria causing the panaxginsen fusarium rot, epidemic disease, sclerotiniose, root rust rot, black spot, rhizoctonia solani, and botrytis. The invention further discloses a plurality of biocontrol mechanisms of the polysporus trichoderma (Hypocrea pachybasioide) FSR-97 for preventing and controlling the plant fungous disease, and an application for preparing a microbial preparation to prevent and control the plant fungous disease.