58 results about "Colony count" patented technology

The invention discloses an aquatic product cold chain transportation monitoring system which is characterized in that the system comprises a hardware layer and a software layer, the hardware layer comprises a sensing control module, a structure bearing module and a communication interaction module, and the software layer comprises a data management module, an intelligent calculation module and a business application module; the sensing control module comprises an environment sensing sub-module and an equipment control sub-module, the structure bearing module comprises a compartment main body sub-module and a cargo protection sub-module, and the communication interaction module comprises an edge calculation sub-module and a vehicle-mounted interaction sub-module. According to the application, a real-time monitoring-dynamic prediction-intelligent regulation and control microbial inhibition system is constructed, a high-precision sensor is used for collecting environmental data in real time, and an LSTM-Attention model is combined for accurately pre-judging the growth trend of microorganisms, so that the effects of early warning before the colony count reaches a dangerous threshold value and automatically coordinating equipment to start targeted bacteriostatic measures are achieved, and the safety of the system is improved. And the loss rate of aquatic products caused by microbial pollution is reduced.

The present invention provides a colony count detection structure, including a colony count detection sheet, an elastic sheet is provided at the right end of the colony count detection sheet, one end of the elastic sheet is fixed on the colony count detection sheet, a first boss and a second boss are provided at the right end of the colony count detection sheet, the elastic sheet is located between the first boss and the second boss, and the other side of the colony count detection sheet is provided with a first bonding area and a second bonding area that can bond the first boss and the second boss, and the left ends of multiple colony count detection sheets are fixedly connected in sequence, and the first and second bosses on the colony count detection sheet of the next layer are bonded to the first bonding area and the second bonding area of ​​the previous layer respectively. When sampling is needed, the present invention tears off the top colony count detection sheet, and at the same time, the next elastic sheet loses the bonding of the previous colony count detection sheet, so that the elastic sheet resumes the effect of tilting, thereby tilting the next colony count detection sheet, so that it is convenient to take it off again.

The invention provides a bacterial colony counting device and a control method. And the burden of counting bacterial colonies by a user is reduced. The colony counting apparatus includes an acquisition section that acquires an image of a colony generated in a test individual, and an execution section that executes first software, the execution section performing a process of counting a number of colonies based on the image of the colony. The execution unit reads a user file that holds information in an array format created by second software different from the first software so as to count colonies generated in the test individual or to manage a result of counting the colonies. Further, the execution section creates a count table for counting colonies based on the user file.

The application relates to the technical field of microorganism detection, and specifically discloses a colony number inversion method based on dynamic conductance response and organic carbon consumption characteristics, which comprises the following steps: obtaining dynamic conductance response data and organic carbon consumption data of a colony growth process in a to-be-detected sample, and synchronously obtaining environmental parameters of the to-be-detected sample; obtaining colony metabolic conductance parameters according to the dynamic conductance response data; obtaining a carbon basis difference value and an organic carbon consumption rate according to the organic carbon concentration data, and obtaining an organic carbon characteristic parameter set according to the carbon basis difference value and the organic carbon consumption rate; and establishing an optimization coefficient matrix according to the environmental parameters and the organic carbon characteristic parameter set. The application synchronously obtains dynamic conductance response data and organic carbon consumption characteristics, combines environmental parameters to establish an optimization coefficient matrix for real-time correction, effectively eliminates environmental interference, and realizes mixed strain identification.

The present invention discloses a biosensor and method for detecting exosomes based on engineered bacteria. The method comprises: magnetic beads modified with recognition elements for binding to exosomes to form a magnetic bead-exosome complex; engineered bacteria modified with recognition elements for binding to the magnetic bead-exosome complex to generate engineered bacteria bound to magnetic beads and exosomes; washing to remove non-target adsorbed engineered bacteria; uniformly coating the engineered bacteria bound to magnetic beads and exosomes on a corresponding culture medium, culturing the culture, and calculating the concentration of the corresponding exosomes by observing the number of colonies. The method is simple to operate, does not require expensive instruments or cumbersome procedures, and can be used for detection using common bacterial culture methods. It can not only detect exosomes from a single source, but also simultaneously detect exosomes from multiple sources by rationally utilizing engineered bacteria containing different resistance genes.

The invention discloses a responsive antibacterial hemicyanine photosensitizer with bacterial division protein FtsZ inhibition capability, and a synthesis method and application thereof. The structure of the responsive antibacterial hemicyanine photosensitizer is shown as a general formula I, the test result of the IHcy-PbE synthesized in the embodiment shows that the IHcy-PbE can inhibit the growth of bacteria under the condition of no light, and can generate active oxygen to kill the bacteria under the light and after being activated by hydrogen peroxide, and the bacterial colony number is obviously reduced. Scanning electron microscope observation shows that bacteria treated by IHcy-PbE and hydrogen peroxide have an obvious splitting blocking condition relative to a control group, and the phenomena of membrane surface collapse and rupture occur in addition to splitting blocking after illumination. The dye not only can realize long-acting bacteriostasis, but also can perform rapid sterilization through photodynamic after responding to a bacterial infection environment, and has a wide application prospect in the fields of bacterial imaging and antibacterial treatment.

The invention relates to the technical field of microbiological detection, and discloses a beverage total colony count real-time early warning system based on impedance microbiological diagnosis, which comprises a data detection module, a colony model establishment module, a feature extraction module, an intelligent analysis module, an early warning output module and a visual interaction module, according to the invention, data is collected in real time and calculated in real time, key control points are continuously and automatically sampled and detected, a production line map generated by the visual interaction module can display a global monitoring state, and a monitoring blind area is eliminated; key characteristic values are automatically extracted from an impedance curve, manual interpretation errors are avoided, a bacterial colony model building module ensures prediction accuracy through rigorous mathematical modeling linear regression and Rverification, and dependence on manpower is reduced; the three characteristic values and the predicted colony number are integrated, four-level grading early warning is carried out, the accelerated growth trend of microorganisms is prompted, and post-event inspection, in-event control and pre-event early warning are achieved.

A method for preparing functional bacterial colonies for carbon fixation and decontamination of organically contaminated soil is provided. The preparation of functional bacterial colonies involves the following steps: S1, culturing the first strain: obtaining single bacteria of Sphingobium sp. RS2; S2, culturing the second strain: obtaining single bacteria of Nitrososphaera viennensis EN76; S3, quantifying the cell count; S4, mixing the single bacteria: mixing the single bacteria of Sphingobium sp. RS2 and the single bacteria of Nitrososphaera viennensis EN76 at a cell count ratio of 1:1 to obtain functional bacterial colonies. [Effects] The obtained functional bacterial colonies are easy to cultivate, easy to formulate, and easy to operate. They can not only purify phenanthrene-contaminated agricultural soil and reduce the threat to agricultural product safety, but also fix CO2 and increase the SOC content in agricultural soil.

The invention provides a low-frequency electric field-based method for measuring and predicting the total bacterial count of litchi epidermis. The method comprises the following steps: establishing a low-frequency electric field detection environment and putting litchis in the environment; applying an excitation electric field with a predetermined low frequency to the litchis; acquiring current environmental parameters and real-time electric signal response generated by the litchi skin; performing feature extraction on the real-time electric signal response by adopting a competitive adaptive reweighted sampling (CARS) algorithm to obtain an electric signal feature reflecting the number of bacterial colonies; inputting the electric signal characteristics into a pre-trained gated circulation unit (GRU) neural network model, and determining the total number of current litchi epidermis bacterial colonies; and predicting the total number of bacterial colonies in at least one measurement period in the future by adopting an autoregressive integral moving average (ARIMA) model in combination with the current and historical environmental parameters and the total number of bacterial colonies. The method realizes rapid, lossless and quantitative determination and dynamic prediction of the total bacterial count of the litchi epidermis, and provides an effective means for quality control and fresh-keeping management of litchis.

The invention discloses a method and a kit for detecting the colony count of inactivated bifidobacterium BL-99 cells before inactivation, and relates to the technical field of instrument analysis. According to the method, at least one of prolyl alanine, citric acid, Asp-Phe peptide, GP (Hyp) GAG peptide, L-methionine, GPRPK peptide and Asp-Leu peptide is taken as a target substance, the colony count of inactivated thalli before inactivation is calculated by testing the content of the target substance, and the detection accuracy is relatively high. A theoretical basis is provided for quantitative detection of the bifidobacterium BL-99 metagen, and industrial standards are also favorably established for quantitative detection of bifidobacterium BL-99 metagen products.

The present invention belongs to the technical field of biopharmaceuticals and specifically relates to use of a pharmaceutical composition in combating mycobacterial infection. The use of zuclopenthixol in combination with bedaquiline exhibits significantly enhanced anti-mycobacterial infection activity. Compared to the use of bedaquiline alone, the use of 2 μg / ml zuclopenthixol in combination with bedaquiline results in varying reductions in colony counts of the MmpL5-MmpS5 strain when the concentration of bedaquiline ranges from 1 / 16 MIC to 2 MIC. As an enhancer of bedaquiline, zuclopenthixol can enhance the anti-mycobacterial infection activity of bedaquiline. The minimum inhibitory concentration of zuclopenthixol against the Rv0678 mutant strain in vitro is 16 μg / ml, and the minimum inhibitory concentration of BDQ against the Rv0678 mutant strain in vitro is 1 μg / mL. The use of 1 μg / ml zuclopenthixol in combination with BDQ reduces the MIC of BDQ against the Rv0678 mutant strain from 1 μg / ml to 0.03 μg / ml (a 32-fold increase in efficacy), which is lower than the MIC against the wild strain H37Rv (0.06 μg / ml), reversing resistance to BDQ and further enhancing the anti-mycobacterial infection activity.

The invention discloses a method and a kit for detecting the colony count of inactivated bacteria of bifidobacterium YLGB-1496 before inactivation, and relates to the technical field of instrument analysis. According to the present invention, at least one of L-methionine, Asp-Phe peptide, prolyl alanine, GPRPK peptide, GP (Hyp) GAG peptide and Asp-Leu peptide is adopted as the target substance, the colony count of the inactivated bacteria before inactivation is calculated by testing the content of the target substance, the detection accuracy is high, the theoretical basis is provided for the quantitative detection of the metagen, and the establishment of the industrial standard for the quantitative detection of the metagen product is easily achieved.

The invention relates to the technical field of microbiological detection, and particularly discloses a bacterial colony number inversion method based on dynamic conductance response and organic carbon consumption characteristics, which comprises the following steps: acquiring dynamic conductance response data and organic carbon consumption data of a bacterial colony growth process in a sample to be detected, and synchronously acquiring environmental parameters of the sample to be detected; acquiring bacterial colony metabolism conductance parameters according to the dynamic conductance response data; acquiring a carbon base difference value and an organic carbon consumption rate according to the organic carbon concentration data, and acquiring an organic carbon characteristic parameter set according to the carbon base difference value and the organic carbon consumption rate; and establishing an optimization coefficient matrix according to the environmental parameters and the organic carbon characteristic parameter set. According to the method, the dynamic conductance response data and the organic carbon consumption characteristics are synchronously obtained, the optimization coefficient matrix is established in combination with the environmental parameters for real-time correction, environmental interference is effectively eliminated, and mixed strain recognition is achieved.

The invention provides a high-purity bovine bone collagen peptide gel filtration chromatography purification method, and belongs to the technical field of bioactive peptide purification. The method comprises the following five core steps: pretreatment, gel column preparation, dynamic equilibrium, sample loading and elution, and post-treatment: firstly, carrying out ultrafiltration pretreatment on a bovine bone collagen peptide crude extract, then filling a chromatographic column with a specific diameter-height ratio by using cross-linked sephadex, carrying out dynamic equilibrium by using an accurately adapted equilibrium liquid after column efficiency verification, and finally, carrying out dynamic equilibrium by using a chromatographic column with a specific diameter-height ratio. The sample loading amount is dynamically matched through a formula, a gradient isothermal mode is adopted in the elution process, and finally a high-purity product is obtained through freeze drying. Through multi-step parameter collaborative optimization and precise regulation and control of a self-created formula, the problems of low purification efficiency, poor product purity and wide molecular weight distribution in the prior art are solved, the purity of the obtained bovine bone collagen peptide is greater than or equal to 98%, the proportion of peptide fragments with molecular weight distribution of 500-3000Da and 1000-2000Da is greater than or equal to 70%, the heavy metal content and the colony count both meet high-standard requirements, the yield is stabilized at 52% or above, and the method is suitable for industrial production. Good industrial application prospects are realized.

The present invention provides a method for detecting the probiotic activity based on multiple strains, including selecting strains to be detected for probiotic activity, the strains including beneficial bacteria and pathogenic bacteria, culturing the selected strains with the prebiotic to be evaluated as the only carbon source of the culture medium, using glucose carbon source culture medium as a positive control group, and sugar-free culture medium as a blank group, obtaining the growth values ​​of all strains and the utilization values ​​of prebiotics, and calculating the probiotic activity. The present invention compares and cultures multiple strains to expand the strain coverage, is more objective and accurate than a single strain, and reduces the contingency and specificity in the detection process. In addition to performing culture detection of multiple groups of probiotics and potential pathogens, the present invention also provides a calculation of the utilization of prebiotics by the strains. At the same time, the present invention uses absorbance at 600nm instead of colony counts for calculation, and calculates the growth value immediately after the strain culture is completed, thereby improving the detection calculation efficiency and facilitating its promotion and application.

The invention discloses a method for evaluating self-cleaning and degerming functions of a washing machine, which comprises the following steps: (1) preparing microbial pollutants and carriers thereof: selecting an aluminum sheet, and uniformly coating the surface of the aluminum sheet with the microbial pollutants to obtain a microbial pollutant carrier; the microbial pollutant carriers are fixed to the center of the bottom of an inner barrel of the washing machine and evenly fixed to the inner wall of the inner barrel at equal intervals, and at least four microbial pollutant carriers are fixed to the inner wall of the inner barrel; starting a self-cleaning program, recovering each microbial pollutant carrier after the self-cleaning program is operated, and measuring the number of bacterial colonies; (3) positive control group test: immobilizing a microbial pollutant carrier in the same way as that of the test group; starting a conventional washing program except for the self-cleaning program, and after the conventional washing program is operated, recycling the microbial pollutant carrier according to a test group method for determining the colony count; and (4) calculating the self-cleaning degerming rate.

The invention discloses a microbial composition for an inoculum for promoting anaerobic biodegradation of plastics, an accelerant, the inoculum and a preparation method of the inoculum, the microbial composition comprises citronellol pseudomonas, copper chloride pseudomonas and pseudomonas putida, and the colony count ratio of the citronellol pseudomonas to the copper chloride pseudomonas to the pseudomonas putida is 1: (1-3): (1-3). The composition is suspended in normal saline or normal saline containing 10% of glycerin to prepare a degradation accelerant, and after the degradation accelerant is inoculated to a standard inoculum and subjected to adaptive culture, the plastic anaerobic biodegradation period can be shortened. According to the method, the anaerobic biodegradation detection time of the anaerobic biodegradation plastic can be remarkably shortened, the detection cost is reduced, and the method has remarkable significance on a detection mechanism and a detection object inspection method.

The invention relates to the technical field of jerky food fermentation, in particular to a pressure and temperature combined control fermentation method and device for jerky food. According to the method, the number of initial probiotic colonies of a sample and fermented probiotic colony data are collected, the rise ratio of the probiotic colonies is calculated, a function relation model between pressure and temperature is established, the optimal fermentation parameter combination and processing time are further determined, and accurate control over the fermentation process is achieved. The device comprises a probiotic bacterial colony detection module, a pressure and temperature regulation and control system and a data processing unit, and can automatically acquire data, perform modeling analysis and execute fermentation operation. According to the method, the scientificity and stability of jerky food fermentation are remarkably improved, the fermentation efficiency is effectively improved, the energy consumption is reduced, the taste damage caused by excessive treatment is avoided, the original flavor and texture are kept while the food safety is guaranteed, and the method is suitable for industrial processing and quality control of middle-end and high-end jerky products.

The invention discloses a handheld water quality bacteria interpretation device and a water quality bacteria inspection box. The handheld water quality bacteria interpretation device comprises a control module, a fluorescence auxiliary module, an imaging system, a display module and a power supply module, the fluorescence auxiliary module is positioned below the imaging system; an ultraviolet mistaken touch prevention module is arranged below the fluorescent auxiliary module; and a water quality bacteria interpretation system is embedded in the control module. The water quality bacterial inspection box comprises a box body, and a handheld interpretation device, an inspection reagent consumable module and an auxiliary instrument module which are arranged in the box body, according to the application, conventional indexes of water quality microorganisms can be detected, intelligent interpretation of a detection result is realized, quantitative interpretation of a bacterial colony total number enzyme substrate method porous quantitative disc result and quantitative / qualitative interpretation of a total coliform / Escherichia coli detection result can be realized; and meanwhile, quantitative interpretation of test piece results such as the total number of bacterial colonies, coliform / escherichia coli and escherichia coli O157 can be realized.

The invention provides an agar method bacterial drug sensitivity automatic reading device and a data analysis method, and belongs to the technical field of microbial devices, and the method specifically comprises the following steps: based on distribution data of observed concentrations and step interval data between the observed concentrations, determining observation duration under the observed concentrations, the method comprises the following steps: determining a secondary test scheme under the observation concentration of secondary observation treatment according to an observation result of a target bacterial colony with the observation concentration within an observation time length, and determining a secondary test scheme under the observation concentration of the secondary observation treatment based on observation results of bacterial colony data under different concentrations in the secondary test scheme and observation results of an original test scheme. And whether the original test scheme needs to be adjusted or not is determined, so that the reliability of the test result is improved.

The invention discloses a novel defensin polypeptide sourced from sclerophyllum squarrosum and application of the novel defensin polypeptide. The novel defensin polypeptide is IpDf3, and the amino acid sequence of the novel defensin polypeptide is shown as SEQ ID NO: 1. The compound has different degrees of antibacterial activity to four gram-positive bacteria and two gram-negative bacteria. Bactericidal kinetics and scanning electron microscope experiments show that the IpDf3 of 3 * MIC rapidly down-regulates the colony count by 1-4 logarithmic units within 60 min, and can cause irregular protrusions or recesses on the surfaces of gram-positive bacteria and even rupture of cell membranes, which indicates that the antibacterial mechanism of the IpDf3 is possibly closely related to direct damage of the cell membranes. The defensin polypeptide not only has the anti-gram-positive bacterium activity of traditional defensin, but also has certain antibacterial activity on gram-negative bacteria, is wide in antibacterial spectrum, high in activity and high in sterilization speed, is expected to provide candidates for research and development of novel antibacterial drugs, and has important application prospects.

The invention relates to the technical field of microorganism culture, and discloses an environment control system for controlling infectious microbe in an enriched microorganism culture process. Comprising an environmental element acquisition and sensing module used for detecting the growth environment of target microorganisms and infectious microbes in real time; the sterilization and pollution control module is used for creating a sterile or low-pollution environment; the data acquisition part is integrated with an IoT (Internet of Things) module and is used for uploading real-time data to a background, and when the infectious microbe level exceeds the standard, the culture breeding is automatically lowered or ultraviolet sterilization is started; and the control module is used for carrying out modular programming on the environment, constructing an anaerobic, aerobic and micro-aerobic condition combination aiming at whether microorganisms are aerobic variables or not, and providing a microorganism culture matrix, distinguishing optimal culture conditions of different floras and constructing a type steady state of enriched microorganisms in cooperation with high-frequency colony count monitoring after flora inoculation.

The invention discloses food safety detection equipment for food microbiological detection, and relates to the technical field of microbiological detection. A box cover is arranged above the base, an extension plate is fixedly connected to the outer side of the bottom surface of the box cover, and a suction cup is arranged on one side of the interior of the extension plate; wherein the middle of the box cover is in threaded connection with a sleeve, the outer side of the sleeve is sleeved with a sealing plug, one side of the sealing plug is connected with a flow dividing pipe in a clamped mode, and the middle of the bottom face of the sleeve is slidably connected with an abutting rod; wherein a contraction groove is formed in the outer side of the top surface of the base, a contraction frame is slidably connected to the middle of the contraction groove, the box cover moves towards one side of the base to achieve unfolding operation of the culture dishes, then gas in the sleeve is pushed through the abutting rod to enter the liquid storage bottle, and reagent dripping can be completed on multiple groups of culture dishes at a time; the time difference of experimental data acquisition is reduced, and convenience is provided for rapid reading of the number of bacterial colonies in food microorganism detection.

The invention relates to the technical field of bioassay, and discloses a total bacterial colony number detection assembly and a bioassay system.The total bacterial colony number detection assembly comprises a sample area, a diluent area, a preparation container area, a carrier area, an incubator, a conveying device and a bacterial colony counting device which are arranged on a platform. A space moving device is arranged on the platform, is connected with the grabbing mechanism and the pipette, and is suitable for driving the grabbing mechanism and the pipette to move in a space above the platform. The grabbing mechanism is suitable for grabbing objects, the pipette is suitable for sucking and discharging liquid, the conveying device is a linear module with a tray arranged on a moving part, the tray is suitable for placing a carrier, and the linear module can drive the tray and the carrier to move between a first position and a second position to enter and exit from the incubator. The total bacterial colony number detection assembly can replace manual work to carry out the partial detection process of the total bacterial colony number, and compared with the mode that the total bacterial colony number is detected completely manually, the working efficiency is higher, and the accuracy is better.

The present invention provides a synchronous detection method for rice molds and aflatoxin B1 contamination based on laser-induced fluorescence technology, including: sample preparation, determination of the content of aflatoxin B1 in rice by an UPLC Xevo TQ liquid chromatography tandem triple quadrupole mass spectrometer, determination of the total number of colonies in rice, acquisition of original laser-induced fluorescence spectral data of rice, extraction of characteristic wavelengths, and construction of discrimination models for toxigenic fungi in rice, mildew degree, and AFB1 content. Based on the average spectral data and fluorescence spectral data corresponding to different relevant characteristic wavelengths, chemometric analysis models for the identification of toxigenic fungi in rice, mildew degree, and AFB1 content discrimination are established synchronously and respectively, to judge whether the contaminated strains in rice have toxigenic ability and whether the total number of colonies and aflatoxin B1 exceed the standards. The present invention will simultaneously detect molds and mycotoxins in rice, providing technical support for the safety of rice during the storage period.

The invention discloses a method for determining the natural transformation efficiency of bacterial drug-resistant plasmids, and belongs to the field of detection technologies. Culturing the natural transformant bacterial liquid of the bacterial drug-resistant plasmid for different times to obtain bacterial liquid at different times; testing the fluorescence intensity and the number of transformed bacterial colonies corresponding to the bacterial liquid at different times, and establishing a first correlation curve between the fluorescence intensity and the number of transformed bacterial colonies; testing the absorbance and the total colony count of the bacterial liquid at different times, and establishing a second correlation curve between the absorbance and the total colony count; taking the to-be-detected bacterial liquid, measuring the fluorescence intensity and absorbance, calculating the number of transformed bacterial colonies of the to-be-detected bacterial liquid according to the fluorescence intensity of the to-be-detected bacterial liquid and the first correlation curve, and calculating the total number of bacterial colonies according to the absorbance of the to-be-detected bacterial liquid and the second correlation curve; and calculating the ratio of the number of transformant colonies / the total colony number to obtain the natural transformation efficiency of the bacterial drug-resistant plasmids. The problem that errors are easily generated by manual counting is solved.

The invention relates to a urinary tract infection model for simulating in-vitro pharmacokinetics / pharmacodynamics of antibacterial drugs. The urinary tract infection model comprises a kidney chamber, a bladder chamber, a drug feeding tank and a drug discharging tank, wherein a bacterial culture medium is arranged in the kidney chamber. The use method of the model comprises the steps of culture medium preparation, drug preparation, strain preparation, flow velocity setting, drug administration simulation and urination simulation. And continuously carrying out continuous medication simulation for 1-7 days, counting the drug concentration and bacteria, and evaluating the action effects of the drug under different doses according to the change of the colony count and the initial bacteria quantity at the observation end point in 24 hours or in 24 hours. The method can be used as a urinary tract infection model for simulating antibacterial drug in-vitro pharmacokinetics / pharmacodynamics research, and PK / PD index and target value research of antibacterial drug urinary tract infection is carried out; evaluating the effectiveness of the antibacterial agent in treating urinary tract infection under the clinical dosage; the clinical treatment effect is improved according to the clinical treatment scheme of the optimized antibacterial medicine; and evaluating the sterilization condition of the antibacterial agent on the bacteria forming the biological membrane in the catheter.

This invention relates to the field of colony identification technology, specifically to a method, system, device, and medium for the fusion processing of static and dynamic colony counts. The method involves static identification, which is performed at least twice to obtain two static colony result images, followed by dynamic identification. Dynamic identification includes: acquiring two adjacent static colony identification results; preprocessing the colony data; and updating the previous dynamic identification result with the next dynamic identification result. This invention mainly combines static and dynamic identification of colony images. The dynamic identification step compares and analyzes the results of two adjacent static identifications, identifying covered colonies or other interfering factors such as impurities, and correcting subsequent dynamic identification. The beneficial effect is that it obtains a more accurate colony count, solving the problem of large counting errors in current technologies.

The invention provides a biogas slurry low-pressure sprinkling irrigation microbial aerosol concentration and particle size testing method and device. The method comprises the following steps: testing the concentration of microorganisms in diluted biogas slurry by adopting a plate coating method; three kinds of low-pressure spray heads are selected, and the mounting height of the spray heads is set to be 1.2 m; the method comprises the following steps: by taking a nozzle as a starting point, arranging microbial aerosol measuring points at intervals of 8m along the radial direction, and selecting the average breathing height 1.64 m of a human body as the collection height at each measuring point; 4, counting microbial colonies on the culture dish by adopting anaerobic fermentation biogas slurry; counting microbial colonies growing on the culture plate by using a colony counting instrument, and correcting the number of the colonies by using a positivity-hole method, so as to obtain the concentration of the air microbial aerosol and the particle size distribution characteristics of the air microbial aerosol. According to the invention, deep research on the dissipation mechanism of the microbial aerosol in the biogas slurry low-pressure sprinkling irrigation process is realized, and a scientific and effective method and system support are provided for parameter optimization and health risk prevention and control of a biogas slurry low-pressure sprinkling irrigation system.

The invention relates to the technical field of household appliances, in particular to purified drinking equipment and a control method for the purified drinking equipment, and aims to solve the problem that the use experience of a user is affected due to the fact that the number of bacterial colonies exceeds the standard due to the fact that a pure water tank of existing purified drinking equipment makes contact with outside air. In order to achieve the purpose, the purified drinking equipment comprises a heating water tank, a purified water tank and a ventilation pipe, a steam cavity is formed in the heating water tank, an air inlet and an air outlet which are communicated with the steam cavity are formed in the heating water tank, a ventilation opening is formed in the purified water tank, one end of the ventilation pipe is communicated with an air outlet, and the other end of the ventilation pipe is communicated with the ventilation opening. And external air enters the steam cavity through the air inlet and flows to the ventilation opening from the air outlet. According to the pure water tank, air sterilized by steam in the steam cavity can flow to the ventilation opening, so that the air entering the pure water tank is clean air sterilized at high temperature, and the situation that the number of bacterial colonies in the pure water tank exceeds the standard due to the fact that external air directly makes contact with the pure water tank is avoided.