15 results about "Colony count" patented technology

The present invention belongs to the technical field of biopharmaceuticals and specifically relates to use of a pharmaceutical composition in combating mycobacterial infection. The use of zuclopenthixol in combination with bedaquiline exhibits significantly enhanced anti-mycobacterial infection activity. Compared to the use of bedaquiline alone, the use of 2 μg / ml zuclopenthixol in combination with bedaquiline results in varying reductions in colony counts of the MmpL5-MmpS5 strain when the concentration of bedaquiline ranges from 1 / 16 MIC to 2 MIC. As an enhancer of bedaquiline, zuclopenthixol can enhance the anti-mycobacterial infection activity of bedaquiline. The minimum inhibitory concentration of zuclopenthixol against the Rv0678 mutant strain in vitro is 16 μg / ml, and the minimum inhibitory concentration of BDQ against the Rv0678 mutant strain in vitro is 1 μg / mL. The use of 1 μg / ml zuclopenthixol in combination with BDQ reduces the MIC of BDQ against the Rv0678 mutant strain from 1 μg / ml to 0.03 μg / ml (a 32-fold increase in efficacy), which is lower than the MIC against the wild strain H37Rv (0.06 μg / ml), reversing resistance to BDQ and further enhancing the anti-mycobacterial infection activity.

The invention relates to the technical field of microbiological detection, and particularly discloses a bacterial colony number inversion method based on dynamic conductance response and organic carbon consumption characteristics, which comprises the following steps: acquiring dynamic conductance response data and organic carbon consumption data of a bacterial colony growth process in a sample to be detected, and synchronously acquiring environmental parameters of the sample to be detected; acquiring bacterial colony metabolism conductance parameters according to the dynamic conductance response data; acquiring a carbon base difference value and an organic carbon consumption rate according to the organic carbon concentration data, and acquiring an organic carbon characteristic parameter set according to the carbon base difference value and the organic carbon consumption rate; and establishing an optimization coefficient matrix according to the environmental parameters and the organic carbon characteristic parameter set. According to the method, the dynamic conductance response data and the organic carbon consumption characteristics are synchronously obtained, the optimization coefficient matrix is established in combination with the environmental parameters for real-time correction, environmental interference is effectively eliminated, and mixed strain recognition is achieved.

The invention provides a high-purity bovine bone collagen peptide gel filtration chromatography purification method, and belongs to the technical field of bioactive peptide purification. The method comprises the following five core steps: pretreatment, gel column preparation, dynamic equilibrium, sample loading and elution, and post-treatment: firstly, carrying out ultrafiltration pretreatment on a bovine bone collagen peptide crude extract, then filling a chromatographic column with a specific diameter-height ratio by using cross-linked sephadex, carrying out dynamic equilibrium by using an accurately adapted equilibrium liquid after column efficiency verification, and finally, carrying out dynamic equilibrium by using a chromatographic column with a specific diameter-height ratio. The sample loading amount is dynamically matched through a formula, a gradient isothermal mode is adopted in the elution process, and finally a high-purity product is obtained through freeze drying. Through multi-step parameter collaborative optimization and precise regulation and control of a self-created formula, the problems of low purification efficiency, poor product purity and wide molecular weight distribution in the prior art are solved, the purity of the obtained bovine bone collagen peptide is greater than or equal to 98%, the proportion of peptide fragments with molecular weight distribution of 500-3000Da and 1000-2000Da is greater than or equal to 70%, the heavy metal content and the colony count both meet high-standard requirements, the yield is stabilized at 52% or above, and the method is suitable for industrial production. Good industrial application prospects are realized.

The invention discloses a method for evaluating self-cleaning and degerming functions of a washing machine, which comprises the following steps: (1) preparing microbial pollutants and carriers thereof: selecting an aluminum sheet, and uniformly coating the surface of the aluminum sheet with the microbial pollutants to obtain a microbial pollutant carrier; the microbial pollutant carriers are fixed to the center of the bottom of an inner barrel of the washing machine and evenly fixed to the inner wall of the inner barrel at equal intervals, and at least four microbial pollutant carriers are fixed to the inner wall of the inner barrel; starting a self-cleaning program, recovering each microbial pollutant carrier after the self-cleaning program is operated, and measuring the number of bacterial colonies; (3) positive control group test: immobilizing a microbial pollutant carrier in the same way as that of the test group; starting a conventional washing program except for the self-cleaning program, and after the conventional washing program is operated, recycling the microbial pollutant carrier according to a test group method for determining the colony count; and (4) calculating the self-cleaning degerming rate.

The invention relates to the technical field of microorganism culture, and discloses an environment control system for controlling infectious microbe in an enriched microorganism culture process. Comprising an environmental element acquisition and sensing module used for detecting the growth environment of target microorganisms and infectious microbes in real time; the sterilization and pollution control module is used for creating a sterile or low-pollution environment; the data acquisition part is integrated with an IoT (Internet of Things) module and is used for uploading real-time data to a background, and when the infectious microbe level exceeds the standard, the culture breeding is automatically lowered or ultraviolet sterilization is started; and the control module is used for carrying out modular programming on the environment, constructing an anaerobic, aerobic and micro-aerobic condition combination aiming at whether microorganisms are aerobic variables or not, and providing a microorganism culture matrix, distinguishing optimal culture conditions of different floras and constructing a type steady state of enriched microorganisms in cooperation with high-frequency colony count monitoring after flora inoculation.

The invention relates to the technical field of bioassay, and discloses a total bacterial colony number detection assembly and a bioassay system.The total bacterial colony number detection assembly comprises a sample area, a diluent area, a preparation container area, a carrier area, an incubator, a conveying device and a bacterial colony counting device which are arranged on a platform. A space moving device is arranged on the platform, is connected with the grabbing mechanism and the pipette, and is suitable for driving the grabbing mechanism and the pipette to move in a space above the platform. The grabbing mechanism is suitable for grabbing objects, the pipette is suitable for sucking and discharging liquid, the conveying device is a linear module with a tray arranged on a moving part, the tray is suitable for placing a carrier, and the linear module can drive the tray and the carrier to move between a first position and a second position to enter and exit from the incubator. The total bacterial colony number detection assembly can replace manual work to carry out the partial detection process of the total bacterial colony number, and compared with the mode that the total bacterial colony number is detected completely manually, the working efficiency is higher, and the accuracy is better.

The invention discloses a method for determining the natural transformation efficiency of bacterial drug-resistant plasmids, and belongs to the field of detection technologies. Culturing the natural transformant bacterial liquid of the bacterial drug-resistant plasmid for different times to obtain bacterial liquid at different times; testing the fluorescence intensity and the number of transformed bacterial colonies corresponding to the bacterial liquid at different times, and establishing a first correlation curve between the fluorescence intensity and the number of transformed bacterial colonies; testing the absorbance and the total colony count of the bacterial liquid at different times, and establishing a second correlation curve between the absorbance and the total colony count; taking the to-be-detected bacterial liquid, measuring the fluorescence intensity and absorbance, calculating the number of transformed bacterial colonies of the to-be-detected bacterial liquid according to the fluorescence intensity of the to-be-detected bacterial liquid and the first correlation curve, and calculating the total number of bacterial colonies according to the absorbance of the to-be-detected bacterial liquid and the second correlation curve; and calculating the ratio of the number of transformant colonies / the total colony number to obtain the natural transformation efficiency of the bacterial drug-resistant plasmids. The problem that errors are easily generated by manual counting is solved.

This invention relates to the field of colony identification technology, specifically to a method, system, device, and medium for the fusion processing of static and dynamic colony counts. The method involves static identification, which is performed at least twice to obtain two static colony result images, followed by dynamic identification. Dynamic identification includes: acquiring two adjacent static colony identification results; preprocessing the colony data; and updating the previous dynamic identification result with the next dynamic identification result. This invention mainly combines static and dynamic identification of colony images. The dynamic identification step compares and analyzes the results of two adjacent static identifications, identifying covered colonies or other interfering factors such as impurities, and correcting subsequent dynamic identification. The beneficial effect is that it obtains a more accurate colony count, solving the problem of large counting errors in current technologies.

The invention provides a biogas slurry low-pressure sprinkling irrigation microbial aerosol concentration and particle size testing method and device. The method comprises the following steps: testing the concentration of microorganisms in diluted biogas slurry by adopting a plate coating method; three kinds of low-pressure spray heads are selected, and the mounting height of the spray heads is set to be 1.2 m; the method comprises the following steps: by taking a nozzle as a starting point, arranging microbial aerosol measuring points at intervals of 8m along the radial direction, and selecting the average breathing height 1.64 m of a human body as the collection height at each measuring point; 4, counting microbial colonies on the culture dish by adopting anaerobic fermentation biogas slurry; counting microbial colonies growing on the culture plate by using a colony counting instrument, and correcting the number of the colonies by using a positivity-hole method, so as to obtain the concentration of the air microbial aerosol and the particle size distribution characteristics of the air microbial aerosol. According to the invention, deep research on the dissipation mechanism of the microbial aerosol in the biogas slurry low-pressure sprinkling irrigation process is realized, and a scientific and effective method and system support are provided for parameter optimization and health risk prevention and control of a biogas slurry low-pressure sprinkling irrigation system.

The invention relates to the technical field of household appliances, in particular to purified drinking equipment and a control method for the purified drinking equipment, and aims to solve the problem that the use experience of a user is affected due to the fact that the number of bacterial colonies exceeds the standard due to the fact that a pure water tank of existing purified drinking equipment makes contact with outside air. In order to achieve the purpose, the purified drinking equipment comprises a heating water tank, a purified water tank and a ventilation pipe, a steam cavity is formed in the heating water tank, an air inlet and an air outlet which are communicated with the steam cavity are formed in the heating water tank, a ventilation opening is formed in the purified water tank, one end of the ventilation pipe is communicated with an air outlet, and the other end of the ventilation pipe is communicated with the ventilation opening. And external air enters the steam cavity through the air inlet and flows to the ventilation opening from the air outlet. According to the pure water tank, air sterilized by steam in the steam cavity can flow to the ventilation opening, so that the air entering the pure water tank is clean air sterilized at high temperature, and the situation that the number of bacterial colonies in the pure water tank exceeds the standard due to the fact that external air directly makes contact with the pure water tank is avoided.

The invention discloses a method for measuring the total number of plate bacterial colonies and application of the method. The determination method comprises the following steps: preparing a plate counting culture medium, and carrying out water bath heat preservation on the plate counting culture medium; the plate counting culture medium contains peptone, yeast extract powder, glucose, agar, sodium pyruvate, L-cysteine hydrochloride, magnesium sulfate and hemin; performing gradient dilution on the target bacteria; fully and uniformly mixing the sample homogenate with a plate counting culture medium; covering the surface of the solidified plate counting culture medium with a layer of same plate counting culture medium to form a covering layer; carrying out constant-temperature culture on the solidified flat plates with various dilution degrees; when macroscopic bacterial colonies are formed in the culture medium plate, selecting a plate with 30-300 bacterial colonies on each plate for counting. The method does not need coating operation, can effectively reduce the pollution risk, shortens the detection time, has clear colonial morphology and reliable data, and is suitable for rapid determination of the total number of colonies in various samples.

This invention relates to the field of microbial detection technology and discloses a method for detecting the colony content of cosmetics based on an intelligent sensing system. The method includes: projecting three-phase phase-shifted structured light onto a culture plate, demodulating to obtain the diffuse reflection alternating component matrix, and calculating the reduced scattering coefficient matrix to decouple static scattering interference; subsequently extracting the true microbial absorption coefficient matrix excluding powder interference, and calculating the speckle time contrast matrix in conjunction with continuously acquired speckle time series; exponentially fusing the two to construct a biochemical and physical activity fusion index matrix, and obtaining the cumulative biomass through local background smoothing and spatial integration; finally, adaptively generating a judgment threshold based on the global data of the culture plate, performing step-constraint screening to remove artifacts, and calculating the absolute total colony count. This invention effectively overcomes the problem of serious early false detections caused by cosmetic matrix artifacts, achieving accurate, closed-loop quantitative identification of live colonies without dependence on external sample libraries.

The aim is to provide a colony counting device and method that enable more accurate judgment regarding the validity of counting results and can efficiently count colonies even in samples where spread colonies have occurred. [Solution] A colony counter 10 for counting colonies, which are aggregates of microorganisms, comprises: a camera 13 for capturing a sample image from a petri dish 1 in which microorganisms are cultured; and a control unit 17 that detects spread colony ICs from the sample image based on the number of pixels obtained by binarizing the sample image captured by the camera 13, and determines the validity of the count result from the ratio of the total pixel value of the detected spread colony ICs to the area of ​​the petri dish 1.

The invention provides a full-automatic bacterial colony counting system and counting method based on a secondary watershed, and belongs to the technical field of microbiological detection and analysis. Comprising a color segmentation module used for converting an input RGB image to an HSV space and separating bacterial colonies with different color features to obtain bacterial colonies with different color features; the adhered bacterial colony separation module is used for separating adhered bacterial colonies from the bacterial colonies with different color characteristics by using a secondary watershed algorithm to obtain separated bacterial colonies; and the morphological characteristic quantitative evaluation module is used for comparing the shape of the separated bacterial colony with a preset standard, removing impurities, and analyzing and evaluating the growth state of the bacterial colony to obtain the number of bacterial colonies meeting the preset standard. According to the method, the color distinguishing capability can be enhanced by utilizing the clustering characteristic of the HSV hue channel; a secondary watershed algorithm is adopted to improve the segmentation precision of the adhered bacterial colonies; bacterial colonies are screened through roundness parameters, and impurity interference is eliminated.