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Preparation method and application of bacillus subtilis for feed

A Bacillus subtilis and feeding technology, which is applied in the field of microbial feed additives and preparation, can solve the problems of low utilization rate of yellow pulp water, environmental pollution, waste of resources, etc. effect of benefit

Pending Publication Date: 2021-12-10
NANJING UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

According to incomplete statistics, the annual production of soybean products in my country is relatively large, but the utilization rate of yellow pulp water in the production process is extremely low. Most of the yellow pulp water and bean dregs are directly discharged and thrown away as waste, causing environmental pollution. Pollution and Waste of Resources
In the current reports, there is no precedent for mixing molasses and bean dregs with Bacillus subtilis to produce feed probiotics

Method used

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  • Preparation method and application of bacillus subtilis for feed

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The inoculation ratios of the seed liquid were respectively inserted into the fermentation medium, and the fermentation experiments were carried out. The inoculation ratios were 1%, 3%, 5%, 7%, and 9%, respectively. 20h, the fermentation medium is bean dregs 21g / L, corn flour 25g / L, ammonium sulfate 6.5g / L, molasses 10g / L, sodium chloride 3g / L, magnesium sulfate 0.25g / L, yeast powder 12g / L, Peptone 3g / L, calcium chloride 0.1g / L, disodium hydrogen phosphate 0.2g / L, sodium dihydrogen phosphate 0.2g / L, yellow syrup water instead of water as liquid supplement, mix and stir, sterilize at 110 °C. The number of colonies of the bacterial agent was determined, and the crude protein results were shown in Table 1.

[0028] Table 1 Effects of different bacterial species ratios on the number of viable bacteria in fermentation

[0029] Seed liquid ratio Total colony count, cfu / mL Crude protein, % 2％ 1.24×10 8

Embodiment 2

[0031] The inoculum amount of 5% (V / V) will be inserted into the fermentation medium, the fermentation temperature is 25 ℃, 30 ℃, 32 ℃, 35 ℃, 37 ℃, 42 ℃, the inoculation amount is 6%, and the cultivation time is 16~ 20h, the fermentation medium is bean dregs 25g / L, corn flour 25g / L, ammonium sulfate 8g / L, molasses 6g / L, sodium chloride 3g / L, magnesium sulfate 0.15g / L, yeast powder 20g / L, peptone 5g / L, calcium chloride 0.5g / L, disodium hydrogen phosphate 0.2g / L, sodium dihydrogen phosphate 0.2g / L, yellow slurry water instead of water as liquid supplement, mix and stir, sterilize at 110°C. , determine the total number of colonies, cfu / mL, crude protein, %, the results are shown in Table 2. Therefore, the optimal fermentation temperature is 37°C.

[0032] Table 2 Effects of different fermentation temperatures on various physical and chemical indicators of fermentation products

[0033] Fermentation temperature, °C Total colony count, cfu / mL Crude protein, % 25...

Embodiment 3

[0035] The present invention investigates a preparation method and application of a forage Bacillus subtilis, and compares the following three culture media: (1) culture medium A: bean dregs 25g / L, corn meal 25g / L, ammonium sulfate 8g / L, molasses 8g / L, sodium chloride 3g / L, magnesium sulfate 0.3g / L, yeast powder 20g / L, peptone 4g / L, calcium chloride 0.5g / L, disodium hydrogen phosphate 0.3g / L, Sodium dihydrogen phosphate 0.3g / L, yellow slurry water instead of water as liquid supplement; (2) Medium B: glucose 20g / L, corn flour 25g / L, ammonium sulfate 8g / L, sodium chloride 3g / L, sulfuric acid Magnesium 0.3g / L, yeast powder 20g / L, peptone 3g / L, calcium chloride 0.6g / L, disodium hydrogen phosphate 0.2g / L, sodium dihydrogen phosphate 0.2g / L, water as liquid supplement,;( 3) Medium C: glucose 30g / L, corn flour 25g / L, ammonium sulfate 10g / L, molasses 8g / L, sodium chloride 3g / L, magnesium sulfate 0.4g / L, yeast powder 20g / L, peptone 5g / L, calcium chloride 0.6g / L, disodium hydrogen ph...

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Abstract

The invention discloses a preparation method and application of bacillus subtilis for feed. The bacillus subtilis is subjected to high-density liquid-state fermentation and then treated, a protective agent is added, and freeze drying is performed to prepare a microbial inoculum serving as a feed additive. In the fermentation process of the strain, the inoculum size of a seed solution is 2-10% (V / V), the strain is inoculated into a fermentation culture medium, the temperature is 25-42 DEG C, fermentation is carried out for 20-48 hours, and the colony count is 5.8*10<10> cfu / g. The fermentation culture medium comprises molasses, corn flour, bean dregs and the like, so that the production cost is remarkably reduced. Compared with a traditional fermentation technology, the colony count of fermentation production is obviously increased, the fermentation period is short, and safety and environment friendliness are achieved; meanwhile, coarse raw materials such as the bean dregs and the molasses are adopted for fermentation production; the bacillus subtilis can be used as the feed additive and has the obvious effects of improving the intestinal environment of livestock and poultry, enhancing animal immunity and promoting animal growth; and the production wastes such as the bean dregs are turned into wealth, so that the recycling of waste resources is realized, the pollution of the waste materials to the environment is reduced, and the production cost is greatly reduced.

Description

technical field [0001] The invention belongs to the technical field of microbial feed additives and preparations, and particularly relates to a preparation method and application of Bacillus subtilis for feed. Background technique [0002] Probiotics are a kind of live bacteria preparations that can improve the growth performance of animals by regulating the balance of microorganisms in the intestinal tract of animals, promoting the growth and reproduction of normal flora in the gastrointestinal tract of animals, and inhibiting the growth and reproduction of pathogenic bacteria groups. Bacteria instead of antibiotics have been widely valued and used as veterinary drugs and feed additives. Bacillus subtilis can improve the balance of the host's intestinal bacterial ecosystem, and effectively decompose the anti-nutritional factors in the bean dregs, so that the nutrients in the fermented bean dregs and yellow pulp water are more balanced. [0003] Bacillus subtilis can compet...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/18C12R1/125
CPCC12N1/20A23K10/18
Inventor 郝宁卢宗梅郭格格周勇李涛陈影庞靖瀛刘利利佟毅仲磊
Owner NANJING UNIV OF TECH
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