83 results about "Colony counts" patented technology

The invention discloses red yeast fish fermented by mixed strains based on lactic acid bacteria, which is made by using fresh fish blocks as a base material, and inoculating the base material with I-type mixed basic bacteria of which the colony count is 10CFUs per gram of fish and II-type mixed basic bacteria of which the colony count is 10spores per gram of fish, and subjecting the inoculated base material to main processing procedures of pickling, fermentation, drying and fumigation to obtain the finished product. The invention adopts a technical proposal that initial culture of microorganism lactic streptococci, lactobacillus bulgaricus and saccharomyces cerevisiae of a single species of the I-type mixed basic bacteria of which the colony count is 10CFUs per gram of fish and initial culture of monascus of the II-type mixed basic bacteria of which the colony count is 10spores per gram of fish are mixed and inoculate with the fish block base material, and the mixture is subjected to pickling, fermentation, drying fumigating and the like to give the red yeast fish blocks, and the technical proposal overcomes the defects that natural fermentation is uneasy to ensure the sanitation and safety of the product and has high salt content. The red yeast fish is suitable for producing various fermented fish blocks.

The invention relates to a composite bacterial agent for deodorization and a preparation method thereof; the composite bacterial agent comprises a bacillus group composed of bacillus subtilis, thiobacillus intermedius, bacillus licheniformis, and a yeast group composed of saccharomyces ellipsoideus and nectaromyces sp. The preparation method comprises the following steps: activating the purchased2 groups of microorganism, preparing a bacterial agent with a total colony count of each group of not less than 1*107 / ml by two steps of seed culture and fermenter culture, uniformly distributing the2-group bacterial agents at different areas of fillers in a biological filter tank for deodorization operations, wherein the bacterial agent comprises by weight 30-50% of bacillus groups and 50-70% of yeast groups, and the two groups are not mixed during distribution. The composite bacterial agent for deodorization prepared by the method provided by the invention has the advantages of good deodorization effect and long deodorization period, and the service time is increased by above 30% when compared with that of agents without the addition of the composite bacterial agent.

The utility model discloses an anti-interference fast detection method and corresponding reagent for total food bacteria amount. First, prepare the food specimen in proper sample solution according to the national standard principle; and add the non-bacteria ATP eliminator to reduce the interferences to the detection from the somatic cell ATP, the dissociated ATP and other impurities; and then carry out ATP luminous detection after orderly adding the bacteria ATP extracting agent and the bioluminescence agent; carry out the ATP standard solution luminous detection at the same time to gain the logarithm relation of the ATP density and the luminous value; at last, combine the luminous value of the food specimen to calculate the ATP density and the total bacteria amount in the sample. The method in the utility model has the advantages that the interferences of the non-bacteria ATP are completely eliminated by chemical agents without the process of bacteria increase or filtration. The method is characterized in convenient operation, rapid detection, high sensitivity, wide detection range and low detection cost; and thus is applicable to rapid detection of the total bacteria amount in food raw material, product and processing device surface as well as in the application of food quality control.

The invention discloses quantitative determination and drug allergy determination kits for helicobacter pylori viable bacteria and a determination method. The method adopts viable helicobacter pylori as a sample to be detected; the property that the viable helicobacter pylori can generate urease is used for rapidly and accurately reading the quantity of the helicobacter pylori by a helicobacter pylori colony counting standard method (CFU / ml, namely the bacterium individual quantity in each ml of bacterium liquid and a colony quantity standard curve); the detection result is accurate and sensitive and has high reliability; the counting difficulty of existing helicobacter pylori clinic microbiological identification, caused by difficult culture and complicated operation, is solved; the drug allergy determination kit and a preparation method, which are expanded by the invention, can be used for carrying out various drug allergy tests at the same time; clinicians can rapidly and conveniently screen suitable anti-helicobacter pylori medicines so that the time and the labor are saved and the cost is saved, so as to provide a beneficial technical solution for clinical scientific treatment.

The invention relates to an ecological microbial inoculum for remedying oil-polluted soils and a preparation method thereof. The ecological microbial inoculum for remedying oil-polluted soils is a mixed microflora of bacillus subtilis, pseudomonas fluorescens, berea trichosporon asahii and candida tropicalis, which are optimally mixed in a ratio of 1:1:1:1 basically. The invention has the advantages that: after the microbial inoculum is applied to the oil-polluted soils, a micro-ecological dominant microflora can be formed rapidly; and in case that the microbial inoculum in an amount which is 2 to 5 percent based on the weight of surface soils (cultivation layer: about 15 to 20cm) is applied to the oil-polluted soils, the oil removing rate is more than or equal to 75 percent (by using the methylene chloride refluxing extraction / gravimetric method) within three months; and the ecological microbial inoculum has a high effective colony count, a strong adaptability, and an obvious effect on remedying the ecological environments of oil-polluted soils.

The invention discloses a high-dietary-fiber bran and red jujube probiotics chewing tablet. The chewing tablet is prepared by pelleting, low-temperature drying, size stabilizing and tabletting of bran and red jujube fermented grain powder, a flavoring agent, a coagulator and a lubricant, wheat bran powder rich in dietary fiber and red jujube mud with full function and high sugar content are taken as raw materials, lactobacillus plantarum and leuconostoc mesenteroides subjected to domestication expanding culture are taken as leavening agents, and the chewing tablet is made through solid state fermentation, vacuum freeze drying and high-speed crushing. The high-dietary-fiber bran and red jujube probiotics chewing tablet has the advantages of easy obtain of the materials, low cost, few addition of supplementary materials, palatable flavor, high dietary fiber content and rich nutrient and intestinal probiotics of the lactobacillus plantarum and leuconostoc mesenteroides, and combined colony count is maintained at 8X107cfu / g above level within the product warranty period; the chewing tablet having good conditioning and healthcare functions on human constipation is a good healthcare food and can be chewed and eaten directly.

The invention relates to a preparation method of a fermentation microbial inoculum for reinforcing and promoting organic fertilizer compost. The method provided by the invention is characterized in that the microbial inoculum of the invention has compost promotion effects on chicken manure, sludge and garbage, and is complex microorganisms composed of bacillus subtilis, Bacillus licheniformis, Rhodopseudomonas palustris, saccharomyces cerevisiae and candida tropicalis with a ratio of 1:1:1:1:1 basically. The method has the active effects that 0.5-1% of microbial inoculum is added in a compost material (chicken manure, sludge or house refuse), and a micro-ecological dominant microflora can be formed rapidly. According to the invention, the odor of a compost material with water content of 50%-60% can be eliminated on the second day (determination by character description) under normal temperature, and the compost process can be completed in 5-7 days. The fermentation microbial inoculum has a high effective colony count and strong adaptability, and has obvious effects for eliminating the odor and promoting the composting process.

The invention relates to a method for exploring indicator bacteria by utilizing n-butyl alcohol oxidizing bacteria as oil-gas microorganism, comprising the following steps of: carrying out block process on a soil sample; coating the soil sample in the hydrocarbon oxidizing bacteria medium by utilizing a spread-plate method in an aseptic operating room; and putting the hydrocarbon oxidizing bacteria medium in a static incubator to cultivate the hydrocarbon oxidizing bacteria, wherein the n-butyl alcohol is used as the unique carbon and energy source of hydrocarbon oxidizing bacteria medium, the cultivating parameter is set as 30 DEG C, 5 days form a cultivating period, and the needed soil sample is air dried rapidly, then dissolved in distilled water and put in a shaking table to activate for use. The method has the advantages of shortening the cultivating period to 5 days, simplifying the whole experiment operating process, enhancing the safety, accurately obtaining the quantity of the needed objective colony by utilizing the medium with n-butyl alcohol as the unique carbon source, and enhancing the reliability of the colony count data through reducing the cultivating time.

The invention discloses staphylococcus aureus bacteriophage and application. The staphylococcus aureus bacteriophage and the application have the advantages that staphylococcus aureus can be specifically effectively eliminated by the staphylococcus aureus bacteriophage, excellent in-vivo and in-vitro antibacterial effects can be realized by the staphylococcus aureus bacteriophage for medicine-resistant staphylococcus aureus, experimental foundations can be provided to clinically developing preparations for preventing and treating medicine-resistant staphylococcus aureus infection, and the staphylococcus aureus bacteriophage has great clinical application potential; the skin abscess average areas of nude mice of bacteriophage treatment groups are 47.32 mm<2> in skin abscess models when MOI(multiplicity of infection) is equal to 10, and the average bacterium load is 3.553*10<7> CFU / g; the skin abscess average areas of nude mice of MRSA (methicillin-resistant staphylococcus aureus) infection groups are 150.4 mm<2>, and the average bacterium load is 2.284*10<8> CFU / g; the skin abscess areas of the mice of the bacteriophage treatment groups are smaller than the skin abscess areas of the mice of the MRSA infection groups, the colony count of the mice of the bacteriophage treatment groups is lower than the colony count of the mice of the MRSA infection groups, and the difference of the skin abscess areas and the colony count has statistical significance.

The invention belongs to the field of microbiology and discloses a method for high throughput screening and separation of bacillus thuringiensis (Bt for short) and application. The method is characterized in that absolute biomass and relative proportion of Bt cells in a sample are greatly improved by selective culture of continuous three-step different strategies; colony counts needing to be picked for transfer culture and microscopic examination are reduced, separating efficiency is greatly improved and greater convenience in operation is realized; meanwhile, the needed sample quantity is reduced and high-throughput operation can be realized; the method is particularly suitable for separating Bt strains from samples with small sample amount or samples with small Bt biomass.

The invention provides a dairy cow early-stage mastitis monitoring method. The method includes following steps: (1), performing SCC count detection on milk; (2), performing pathogenic microorganism identification of the milk: colony count and bacteriological identification; (3), performing somatic cell typing detection; (4), identifying protein differential expression level and function of the milk: protein concentration measuring, protein quantifying and analysis protein differential expression level and function identification. By detecting differentiation degree of immune cells in cow's milk somatic cells and identifying expression level and function of mycoprotein in the milk, the method is conducive to timely finding early-stage mastitis and pathogen types inducing early-stage inflammation to make full preparation for timely prevention and targeted treatment of dairy cow mastitis.

The invention discloses a fermented milk sampling infectious microbe detection device for production. The device comprises a base and a mounting plate fixed on the base, the mounting plate is provided with a liquid strain mixing part and a gas strain culture part, the mounting plate is provided with a constant temperature box for culturing a strain culture mixed solution at a constant temperature, the mounting plate is provided with a detection part used for observing the number of infectious microbe colonies, the liquid strain mixing part is used for mixing fermented milk liquid and a culture solution, the mixed culture mixed solution is subjected to constant-temperature culture in a constant-temperature box, and the gas strain culture part is used for performing constant-temperature culture on a mixed solution of gas and the culture solution, and the detection part is used for observing the colony count of the liquid strain mixed solution and the gas strain mixed solution, observing whether bacterial contamination exists or not and judging the bacterial type. The device can perform pretreatment and detection on a sample, the whole process is continuous, sampling detection of infectious microbe by experimenters is greatly facilitated, and efficiency is high.

The invention discloses a method for optimizing and recycling waste drilling fluid. The method for optimizing and recycling the waste drilling fluid comprises a waste drilling fluid maintaining and reserving step. The waste drilling fluid maintaining and reserving step refers to preparing 0.1-0.3 percent of sterilizing agent, 0.1-0.2 percent of deoxidant, 0.1-0.2 percent of basicity control agent and 0.05-0.2 percent of dispersing agent for later use by accounting for the mass percent of the waste drilling fluid. The waste drilling fluid maintaining and reserving step is performed at normal temperature and pressure according to the following procedures of: a, adding 0.1-0.3 percent of sterilizing agent to the waste drilling fluid every four days and performing a total bacterial count testing experiment by using a 3M testing slip; b, adding 0.1-0.2 percent of deoxidant to the waste drilling fluid every five days; c, adding 0.1-0.2 percent of basicity control agent to the waste drilling fluid every seven days and measuring the potential of hydrogen (pH) value of the waste drilling fluid to keep the pH between 10 and 11; and d, adding 0.05-0.2 percent of dispersing agent to the waste drilling fluid every seven days. The method is especially suitable for drilling operations of a horizontal well; the ambient pressure is greatly relieved and the cost of drilling fluid is reduced; and the problem that micron-sized and submicron-sized solid phases in the drilling fluid cannot be effectively removed in the prior art is solved.

The invention provides a method for preparing catalase by using bovine livers, wherein fresh bovine livers are used as raw materials and subjected to chopping and homogenization, and a catalase product with high enzyme activity is obtained by means of processes such as ultrasonic extraction, ion exchange chromatography, gel filtration chromatography and freeze drying. The method of the invention solves the problems of complex production process, heavy pollution, high production cost and low product yield of the prior art in preparing the product. The catalase prepared by the method of the invention has an enzyme activity of 6000 U / mg and a colony count of less than or equal to 1000 cells / ml, and is suitable to be widely used in the fields of food, paper making and textile industry.

The invention discloses a preparation method of a high-activity microencapsulated lactic acid bacteria starter. The preparation method comprises the following steps: inoculating lactic acid bacteria to a culture medium to be cultured; filtering the bacterial liquid with a hollow fiber membrane, and concentrating to form a concentrated bacterial liquid; injecting a fresh culture medium into the concentrated bacterial liquid to be cultured; filtering the prepared bacterial liquid with the hollow fiber membrane and concentrating for 2-3 times to obtain a concentrated bacterial liquid with a unit colony count up to 1,011 cfu / ml above; adding gelatin, sucrose and whey protein into the concentrated bacterial liquid as wall materials; carrying out spray drying to obtain the microencapsulated lactic acid bacteria starter. According to the preparation method disclosed by the invention, the hollow fiber membrane is combined with a bioreactor to carry out high-density culture and concentration of the lactic acid bacteria, so as to implement online continuous filtration, concentration and culture and avoid pollution due to thallus transfer; the coupled spray drying at a low temperature is carried out to effectively protect the concentrated high-density bacteria, so as to obtain the lactic acid bacteria starter with a unit viable count up to 1*10<11>-1*10<12> cfu / ml.

The invention discloses a method for measuring the content of live spores in a fungal microbial pesticide quickly. The method comprises the following steps of: (1) adding 0.25 to 5 weight percent of sodium deoxycholate into a fungal culture medium, and preparing flat plates; (2) soaking fungal microbial pesticide samples in sterilization water containing Tween 20 or polyethylene glycol octylphenol ether, and shaking; (3) diluting in a multiple gradient mode, coating the samples of different dilutability on the flat plate respectively and uniformly, and culturing at room temperature for 48 to 120 hours; (4) counting colony counts, wherein the flat plates on which the colony counts are 30 to 300 are effectively-counted flat plates; and (5) counting the content of the live spores, namely C is equal to T*N*10, wherein in a formula, C is the content of the fungal pesticide live spores, the unit is colony forming unit (CFU) / gram, T is dilution times corresponding to the counted flat plates, and N is average colony counts on a plurality of effectively-counted flat plates of the same dilution times. By the method, the content of the live spores of the fungal microbial pesticide can be measured simply, conveniently and quickly, and the detection accuracy is high.

The invention provides an indoor ultraviolet sterilization and disinfection regulation and control system based on the Internet of Things. The system comprises an input end which is used for monitoring whether someone enters and exits a room or not, collecting the total number of bacteria in indoor air, and sending information about whether someone enters and exits the room and information about the total number of bacteria in the air to a control end; the control end is used for receiving the information sent by the input end, judging whether to start the action end or not according to the information and sending a corresponding signal to the action end; and the action end is used for receiving the signal sent by the control end and giving an alarm or disinfecting the indoor space according to the signal. The system is combined with the Internet of Things technology, the intelligence and safety of the system are improved, whether to disinfect or sterilize the indoor environment or notcan be determined according to the actual situation, and the intelligence of the system conforms to the trend of scientific and technological development of the current society; the safety meets therequirements of people for high-quality life nowadays; the practicability meets the requirements of actual life, and the device can be widely applied to various places.

The invention discloses a straw low temperature degradation acidification microbial agent. The straw low temperature degradation acidification microbial agent contains a bacterial strain and an adsorption carrier, wherein the bacterial strain contains aminobacterium mobile sp, clostridium cellulolyticum, clostridium xylanolyticum and bacillus cereus, wherein the colony count of aminobacterium mobile sp is 33-36, the colony count of clostridium cellulolyticum is 33-36, the colony count of clostridium xylanolyticum is 18-25, and the colony count of bacillus cereus is 5-15; the adsorption carrier is prepared by mixing medical stones and straw powder; and the ratio of the total number of effective live bacteria to the mass of the absorption carrier in the straw low temperature degradation acidification microbial agent is 1*10<5>-1*10<6>: 1g of adsorption carrier. The straw low temperature degradation acidification microbial agent can quickly decompose celluloses and hemicelluloses at a low temperature and degrade the celluloses and the hemicelluloses into acetic acid, thereby increasing biogas production efficiency. In addition, the invention also provides a preparation method and application of the straw low temperature degradation acidification microbial agent.

The invention discloses a preparation method and application of bacillus subtilis for feed. The bacillus subtilis is subjected to high-density liquid-state fermentation and then treated, a protective agent is added, and freeze drying is performed to prepare a microbial inoculum serving as a feed additive. In the fermentation process of the strain, the inoculum size of a seed solution is 2-10% (V / V), the strain is inoculated into a fermentation culture medium, the temperature is 25-42 DEG C, fermentation is carried out for 20-48 hours, and the colony count is 5.8*10<10> cfu / g. The fermentation culture medium comprises molasses, corn flour, bean dregs and the like, so that the production cost is remarkably reduced. Compared with a traditional fermentation technology, the colony count of fermentation production is obviously increased, the fermentation period is short, and safety and environment friendliness are achieved; meanwhile, coarse raw materials such as the bean dregs and the molasses are adopted for fermentation production; the bacillus subtilis can be used as the feed additive and has the obvious effects of improving the intestinal environment of livestock and poultry, enhancing animal immunity and promoting animal growth; and the production wastes such as the bean dregs are turned into wealth, so that the recycling of waste resources is realized, the pollution of the waste materials to the environment is reduced, and the production cost is greatly reduced.

The invention provides application of TCC to prevention of colony diffuse growth in colony count determination. The method includes: taking Bacillus cereus, Escherichia coli and Staphylococcus aureusof appropriate dilution, adding 0.01%, 0.05%, 0.08%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.8%, and 1.0% TTC aqueous solution plate count agar, at the same time taking count agar without TTC as control, andobserving the growth condition of bacteria. In colony count determination, if a sample possibly contains Bacillus colonies growing diffusely on an agar medium surface, TTC with a concentration of 0.2%-0.3% can be added to the agar to prevent incalculability situation caused by diffusion of colonies.

The invention discloses a method for decontaminating dehydrated garlic products. The method for manufacturing the dehydrated garlic products includes steps of soaking and disinfecting selected garlicin acidic electrolytic water with the pH (potential of hydrogen) value of 2-4 for 5-15 min and then cleaning the garlic by the aid of pure water; placing the garlic in ozone water, carrying out cooling treatment on the garlic for 5-10 minutes and splitting the garlic; secondarily treating split raw materials by the aid of ozone water for 20-40 minutes; draining raw materials treated by the aid ofthe ozone water, then carrying out stepped temperature control drying on the raw materials, in other words, drying the raw materials at the temperatures of 110-120 DEG C for 1-2 hours, gradually reducing the temperatures until the temperatures reach 60-80 DEG C, and continuing to dry the raw materials until the moisture contents of the raw materials are reduced and reach 5-8%. The method has the advantages that the quantity of germs carried by the garlic can be reduced by 60%-67%, late sterilization can be omitted, the sterilization intensity of the method is far lower than the sterilization intensity of conventional processing technologies, dehydrated garlic which is subjected to contamination treatment by the aid of the method is little in color and nutritional component change, but thetotal colony count of the dehydrated garlic can be obviously reduced and reaches high standards, and the problem of dull sale due to the fact that the total colony count of existing garlic products exceeds standards can be effectively solved by the aid of the method.

The invention relates to a microbial colony counting instrument which comprises a sample layer, an amplification layer and a shooting layer which are sequentially arranged from bottom to top, and further comprises a first light shield; the amplification layer comprises a mounting plate, and a plurality of microscope assemblies are arranged on the mounting plate; the shooting layer comprises a shooting camera, a shooting turntable and a rotating assembly; the sample layer comprises a plurality of sample index plates, and a plurality of sample grooves are annularly distributed in the sample index plates; and a second light shield is arranged in the first light shield, and the second light shield is longitudinally and slidably connected to the inner top of the first light shield. By means of the method, batch automatic detection can be conducted after one-time feeding, external light interference is reduced to the minimum through the double light shields, and the peripheral light shields are used for shielding light and serve as supporting brackets of the overall structure; and meanwhile, only one shooting camera is needed, the overall structure is reasonable and compact, the detection efficiency is high, the automation degree is high, and the cost is low.

The invention discloses a use of a rheum officinale monomer in the preparation of medicines for inhibiting Streptococcus suis or intervening a Streptococcus suis biofilm, and belongs to the field of new medicinal uses of the rheum officinale monomer. A biofilm model is established, and crystal violet staining and colony count detection results show that the rheum officinale monomer has an inhibition effect on the formation of the Streptococcus suis biofilm. A fluorescent quantitative PCR detection result shows the influence of emodin on the expression level of mRNA of virulence genes and signaling molecules in the Streptococcus suis biofilm is a dose effect relationship, and the expression of the mRNA of respective virulence genes and the signaling molecules significantly decreases. The rheum officinale monomer can definitely inhibit the Streptococcus suis or intervene the Streptococcus suis biofilm, can be applied to prepare medicines for inhibiting the Streptococcus suis, intervening the formation of the Streptococcus suis biofilm or intervening the expression of the virulence genes or signaling molecules in the Streptococcus suis biofilm. A new treatment way is provided for Streptococcus suis biofilm related infections.

The invention discloses a colony count alarming device for vessel ballast water, and relates to the technical field of ocean vessel transportation and offshore oil engineering. The alarming device comprises a base, a sample storage tank, a pressure detection unit, a turbidity detection unit, a fluorescence self-illumination detection unit and a comprehensive alarming unit, wherein the pressure detection unit, the turbidity detection unit and the fluorescence self-illumination detection unit are detection units and detect concentration of colony counts in the ballast water through energy detection of aerobic bacteria utilizing oxygen, ballast water turbidity detection and biological self-illumination detection respectively, and the comprehensive alarming unit is used for collecting and processing signals of the three detection units, and two of the three signals are output in an acoustic-optical manner for alarming. The overall device is high in redundancy and high in reliability and has the characteristics of compact size, convenience in detection, economical efficiency and practicability.

The invention discloses an inoculation device for measuring the total number of bacteria in sewage. The inoculation device comprises a workbench, wherein an inoculation cavity with a forward opening is arranged in the workbench; an inoculation unit is arranged in the inoculation cavity and used for conveying sewage and molten agar onto a culture dish, so inoculation movement required for detectionof bacteria is realized; an uncovering and sealing unit located at the lower side of the inoculation unit is arranged in the inoculation cavity; a transverse moving unit is arranged on the inner wallof the upper side of the inoculation cavity; a power cavity located at the lower side of the inoculation cavity is arranged in the workbench and communicates with the inoculation cavity; a rotating unit is arranged in the power cavity and used for providing power for rotating movement of the transverse moving unit. The inoculation device provided by the invention can automatically perform the inoculation movement required by measuring of the total number of the bacteria in the sewage, so the inoculation efficiency is improved, and the technical requirements on an operator are reduced.

The invention discloses a rapid lossless fresh pork shelf life evaluation method and a detecting system adopting the evaluation method. The detecting system is composed of a computer and a light spectrum information acquisition device, wherein a detection control analysis software system is mounted in the computer; a light spectrum data preprocessing algorithm routine, a total bacterial count light spectrum prediction model and an anti regression equation model of total bacterial count and shelf life are prestored in the detection control analysis software system; the detection control analysis software system can automatically preprocess the gathered light spectrum data, inputs the gathered light spectrum data as input variable into the total bacterial count light spectrum prediction model, predicts the total bacterial count in a pork sample, inputs the predicted value of the predicted total bacterial count in the anti regression equation model of the shelf life as the input variable, and automatically predicts the shelf life of the pork sample. Lossless detection can be realized, the detection speed and the efficiency are high, the cost is reduced, and the detecting system can be applicable to various occasions such as portable detection, online detection and the like.

A method of measuring simply and accurately a microbial count in a sample. Namely, a method of measuring a microbial count including a step of blending a composition for preparing a culture medium for a microbial count measurement including (a) a polymer able to form a non-flowable transparent gel without a melting step by heating and without cooling, and (b) a nutrient component, with a sample added thereto, a step of incubating microorganisms contained in the sample, and a step of measuring the colony count of the microorganisms.

The invention belongs to the technical field of compound materials, and particularly relates to a fluorescence detection method of a total number of living bacteria. The fluorescence detection methodof the total number of the living bacteria comprises the following steps: conducting a mixing reaction on a to-be-detected sample, with an unknown number of the living bacteria, and fluorescent dye toobtain to-be-detected reaction liquid subjected to fluorescence staining; and detecting relative fluorescence intensity of the to-be-detected reaction liquid to obtain the number of the living bacteria in the to-be-detected reaction liquid. By means of the fluorescence detection method of the total number of the living bacteria, the number of the living bacteria which actually exist in an environment can be reflected, and the fluorescence detection method of the total number of the living bacteria is high in detection speed, convenient, simple, easy to operate, high in sensibility, accurate and good in dynamic range, can detect multiple kinds of microorganisms, and can achieve field monitoring.