35 results about "Colony counts" patented technology

The present invention provides a colony count detection structure, including a colony count detection sheet, an elastic sheet is provided at the right end of the colony count detection sheet, one end of the elastic sheet is fixed on the colony count detection sheet, a first boss and a second boss are provided at the right end of the colony count detection sheet, the elastic sheet is located between the first boss and the second boss, and the other side of the colony count detection sheet is provided with a first bonding area and a second bonding area that can bond the first boss and the second boss, and the left ends of multiple colony count detection sheets are fixedly connected in sequence, and the first and second bosses on the colony count detection sheet of the next layer are bonded to the first bonding area and the second bonding area of ​​the previous layer respectively. When sampling is needed, the present invention tears off the top colony count detection sheet, and at the same time, the next elastic sheet loses the bonding of the previous colony count detection sheet, so that the elastic sheet resumes the effect of tilting, thereby tilting the next colony count detection sheet, so that it is convenient to take it off again.

The invention provides a bacterial colony counting device and a control method. And the burden of counting bacterial colonies by a user is reduced. The colony counting apparatus includes an acquisition section that acquires an image of a colony generated in a test individual, and an execution section that executes first software, the execution section performing a process of counting a number of colonies based on the image of the colony. The execution unit reads a user file that holds information in an array format created by second software different from the first software so as to count colonies generated in the test individual or to manage a result of counting the colonies. Further, the execution section creates a count table for counting colonies based on the user file.

The invention discloses a method and a kit for detecting the colony count of inactivated bifidobacterium BL-99 cells before inactivation, and relates to the technical field of instrument analysis. According to the method, at least one of prolyl alanine, citric acid, Asp-Phe peptide, GP (Hyp) GAG peptide, L-methionine, GPRPK peptide and Asp-Leu peptide is taken as a target substance, the colony count of inactivated thalli before inactivation is calculated by testing the content of the target substance, and the detection accuracy is relatively high. A theoretical basis is provided for quantitative detection of the bifidobacterium BL-99 metagen, and industrial standards are also favorably established for quantitative detection of bifidobacterium BL-99 metagen products.

The present invention belongs to the technical field of biopharmaceuticals and specifically relates to use of a pharmaceutical composition in combating mycobacterial infection. The use of zuclopenthixol in combination with bedaquiline exhibits significantly enhanced anti-mycobacterial infection activity. Compared to the use of bedaquiline alone, the use of 2 μg / ml zuclopenthixol in combination with bedaquiline results in varying reductions in colony counts of the MmpL5-MmpS5 strain when the concentration of bedaquiline ranges from 1 / 16 MIC to 2 MIC. As an enhancer of bedaquiline, zuclopenthixol can enhance the anti-mycobacterial infection activity of bedaquiline. The minimum inhibitory concentration of zuclopenthixol against the Rv0678 mutant strain in vitro is 16 μg / ml, and the minimum inhibitory concentration of BDQ against the Rv0678 mutant strain in vitro is 1 μg / mL. The use of 1 μg / ml zuclopenthixol in combination with BDQ reduces the MIC of BDQ against the Rv0678 mutant strain from 1 μg / ml to 0.03 μg / ml (a 32-fold increase in efficacy), which is lower than the MIC against the wild strain H37Rv (0.06 μg / ml), reversing resistance to BDQ and further enhancing the anti-mycobacterial infection activity.

A system for detecting a target bacteria is disclosed. The system comprises a flow cytometer. The flow cytometer is configured to receive a fluid sample, wherein the fluid sample comprises at least a target bacteria and at least a contaminant bacteria. The flow cytometer is also configured to generate a first enumeration of a total bacteria in the fluid sample during a pre-incubation phase. The fluid sample is then incubated during an incubation phase. The flow cytometer then generates a second enumeration of the total bacteria in the fluid sample during a post-incubation phase. A computing device then determines a growth ratio of the total bacteria as a function of the first enumeration and the second enumeration. Finally, the computing device identifies the presence of the at least a target bacteria as a function of the growth ratio.

The invention provides a high-purity bovine bone collagen peptide gel filtration chromatography purification method, and belongs to the technical field of bioactive peptide purification. The method comprises the following five core steps: pretreatment, gel column preparation, dynamic equilibrium, sample loading and elution, and post-treatment: firstly, carrying out ultrafiltration pretreatment on a bovine bone collagen peptide crude extract, then filling a chromatographic column with a specific diameter-height ratio by using cross-linked sephadex, carrying out dynamic equilibrium by using an accurately adapted equilibrium liquid after column efficiency verification, and finally, carrying out dynamic equilibrium by using a chromatographic column with a specific diameter-height ratio. The sample loading amount is dynamically matched through a formula, a gradient isothermal mode is adopted in the elution process, and finally a high-purity product is obtained through freeze drying. Through multi-step parameter collaborative optimization and precise regulation and control of a self-created formula, the problems of low purification efficiency, poor product purity and wide molecular weight distribution in the prior art are solved, the purity of the obtained bovine bone collagen peptide is greater than or equal to 98%, the proportion of peptide fragments with molecular weight distribution of 500-3000Da and 1000-2000Da is greater than or equal to 70%, the heavy metal content and the colony count both meet high-standard requirements, the yield is stabilized at 52% or above, and the method is suitable for industrial production. Good industrial application prospects are realized.

The present invention provides a method for detecting the probiotic activity based on multiple strains, including selecting strains to be detected for probiotic activity, the strains including beneficial bacteria and pathogenic bacteria, culturing the selected strains with the prebiotic to be evaluated as the only carbon source of the culture medium, using glucose carbon source culture medium as a positive control group, and sugar-free culture medium as a blank group, obtaining the growth values ​​of all strains and the utilization values ​​of prebiotics, and calculating the probiotic activity. The present invention compares and cultures multiple strains to expand the strain coverage, is more objective and accurate than a single strain, and reduces the contingency and specificity in the detection process. In addition to performing culture detection of multiple groups of probiotics and potential pathogens, the present invention also provides a calculation of the utilization of prebiotics by the strains. At the same time, the present invention uses absorbance at 600nm instead of colony counts for calculation, and calculates the growth value immediately after the strain culture is completed, thereby improving the detection calculation efficiency and facilitating its promotion and application.

The invention discloses a microbial composition for an inoculum for promoting anaerobic biodegradation of plastics, an accelerant, the inoculum and a preparation method of the inoculum, the microbial composition comprises citronellol pseudomonas, copper chloride pseudomonas and pseudomonas putida, and the colony count ratio of the citronellol pseudomonas to the copper chloride pseudomonas to the pseudomonas putida is 1: (1-3): (1-3). The composition is suspended in normal saline or normal saline containing 10% of glycerin to prepare a degradation accelerant, and after the degradation accelerant is inoculated to a standard inoculum and subjected to adaptive culture, the plastic anaerobic biodegradation period can be shortened. According to the method, the anaerobic biodegradation detection time of the anaerobic biodegradation plastic can be remarkably shortened, the detection cost is reduced, and the method has remarkable significance on a detection mechanism and a detection object inspection method.

The invention relates to the technical field of jerky food fermentation, in particular to a pressure and temperature combined control fermentation method and device for jerky food. According to the method, the number of initial probiotic colonies of a sample and fermented probiotic colony data are collected, the rise ratio of the probiotic colonies is calculated, a function relation model between pressure and temperature is established, the optimal fermentation parameter combination and processing time are further determined, and accurate control over the fermentation process is achieved. The device comprises a probiotic bacterial colony detection module, a pressure and temperature regulation and control system and a data processing unit, and can automatically acquire data, perform modeling analysis and execute fermentation operation. According to the method, the scientificity and stability of jerky food fermentation are remarkably improved, the fermentation efficiency is effectively improved, the energy consumption is reduced, the taste damage caused by excessive treatment is avoided, the original flavor and texture are kept while the food safety is guaranteed, and the method is suitable for industrial processing and quality control of middle-end and high-end jerky products.

The invention discloses a handheld water quality bacteria interpretation device and a water quality bacteria inspection box. The handheld water quality bacteria interpretation device comprises a control module, a fluorescence auxiliary module, an imaging system, a display module and a power supply module, the fluorescence auxiliary module is positioned below the imaging system; an ultraviolet mistaken touch prevention module is arranged below the fluorescent auxiliary module; and a water quality bacteria interpretation system is embedded in the control module. The water quality bacterial inspection box comprises a box body, and a handheld interpretation device, an inspection reagent consumable module and an auxiliary instrument module which are arranged in the box body, according to the application, conventional indexes of water quality microorganisms can be detected, intelligent interpretation of a detection result is realized, quantitative interpretation of a bacterial colony total number enzyme substrate method porous quantitative disc result and quantitative / qualitative interpretation of a total coliform / Escherichia coli detection result can be realized; and meanwhile, quantitative interpretation of test piece results such as the total number of bacterial colonies, coliform / escherichia coli and escherichia coli O157 can be realized.

The invention discloses a novel defensin polypeptide sourced from sclerophyllum squarrosum and application of the novel defensin polypeptide. The novel defensin polypeptide is IpDf3, and the amino acid sequence of the novel defensin polypeptide is shown as SEQ ID NO: 1. The compound has different degrees of antibacterial activity to four gram-positive bacteria and two gram-negative bacteria. Bactericidal kinetics and scanning electron microscope experiments show that the IpDf3 of 3 * MIC rapidly down-regulates the colony count by 1-4 logarithmic units within 60 min, and can cause irregular protrusions or recesses on the surfaces of gram-positive bacteria and even rupture of cell membranes, which indicates that the antibacterial mechanism of the IpDf3 is possibly closely related to direct damage of the cell membranes. The defensin polypeptide not only has the anti-gram-positive bacterium activity of traditional defensin, but also has certain antibacterial activity on gram-negative bacteria, is wide in antibacterial spectrum, high in activity and high in sterilization speed, is expected to provide candidates for research and development of novel antibacterial drugs, and has important application prospects.

The invention relates to the technical field of bioassay, and discloses a total bacterial colony number detection assembly and a bioassay system.The total bacterial colony number detection assembly comprises a sample area, a diluent area, a preparation container area, a carrier area, an incubator, a conveying device and a bacterial colony counting device which are arranged on a platform. A space moving device is arranged on the platform, is connected with the grabbing mechanism and the pipette, and is suitable for driving the grabbing mechanism and the pipette to move in a space above the platform. The grabbing mechanism is suitable for grabbing objects, the pipette is suitable for sucking and discharging liquid, the conveying device is a linear module with a tray arranged on a moving part, the tray is suitable for placing a carrier, and the linear module can drive the tray and the carrier to move between a first position and a second position to enter and exit from the incubator. The total bacterial colony number detection assembly can replace manual work to carry out the partial detection process of the total bacterial colony number, and compared with the mode that the total bacterial colony number is detected completely manually, the working efficiency is higher, and the accuracy is better.

The invention discloses a water sample automatic culture and total bacterial count determination system and method, the system comprises a shell and a central control unit, the interior of the shell is divided into a to-be-detected sample area, a pretreatment area and a bacterial culture counting area through a lifting partition plate, and the central control unit is connected with the control end of the lifting partition plate; the system and the method can realize the automation of the whole process from pretreatment, dilution, inoculation, constant-temperature culture to colony counting of the water sample, improve the detection efficiency and the reliability of the result, and reduce the workload of personnel.

The invention discloses a method for determining the natural transformation efficiency of bacterial drug-resistant plasmids, and belongs to the field of detection technologies. Culturing the natural transformant bacterial liquid of the bacterial drug-resistant plasmid for different times to obtain bacterial liquid at different times; testing the fluorescence intensity and the number of transformed bacterial colonies corresponding to the bacterial liquid at different times, and establishing a first correlation curve between the fluorescence intensity and the number of transformed bacterial colonies; testing the absorbance and the total colony count of the bacterial liquid at different times, and establishing a second correlation curve between the absorbance and the total colony count; taking the to-be-detected bacterial liquid, measuring the fluorescence intensity and absorbance, calculating the number of transformed bacterial colonies of the to-be-detected bacterial liquid according to the fluorescence intensity of the to-be-detected bacterial liquid and the first correlation curve, and calculating the total number of bacterial colonies according to the absorbance of the to-be-detected bacterial liquid and the second correlation curve; and calculating the ratio of the number of transformant colonies / the total colony number to obtain the natural transformation efficiency of the bacterial drug-resistant plasmids. The problem that errors are easily generated by manual counting is solved.

This invention relates to the field of colony identification technology, specifically to a method, system, device, and medium for the fusion processing of static and dynamic colony counts. The method involves static identification, which is performed at least twice to obtain two static colony result images, followed by dynamic identification. Dynamic identification includes: acquiring two adjacent static colony identification results; preprocessing the colony data; and updating the previous dynamic identification result with the next dynamic identification result. This invention mainly combines static and dynamic identification of colony images. The dynamic identification step compares and analyzes the results of two adjacent static identifications, identifying covered colonies or other interfering factors such as impurities, and correcting subsequent dynamic identification. The beneficial effect is that it obtains a more accurate colony count, solving the problem of large counting errors in current technologies.

The invention provides a biogas slurry low-pressure sprinkling irrigation microbial aerosol concentration and particle size testing method and device. The method comprises the following steps: testing the concentration of microorganisms in diluted biogas slurry by adopting a plate coating method; three kinds of low-pressure spray heads are selected, and the mounting height of the spray heads is set to be 1.2 m; the method comprises the following steps: by taking a nozzle as a starting point, arranging microbial aerosol measuring points at intervals of 8m along the radial direction, and selecting the average breathing height 1.64 m of a human body as the collection height at each measuring point; 4, counting microbial colonies on the culture dish by adopting anaerobic fermentation biogas slurry; counting microbial colonies growing on the culture plate by using a colony counting instrument, and correcting the number of the colonies by using a positivity-hole method, so as to obtain the concentration of the air microbial aerosol and the particle size distribution characteristics of the air microbial aerosol. According to the invention, deep research on the dissipation mechanism of the microbial aerosol in the biogas slurry low-pressure sprinkling irrigation process is realized, and a scientific and effective method and system support are provided for parameter optimization and health risk prevention and control of a biogas slurry low-pressure sprinkling irrigation system.

The invention belongs to the technical field of biological medicine and molecular biology, and provides application of S100A8 in preparation of a medicine for preventing / treating bladder infection after spinal cord injury. The S100A8 can be used as a prevention target of bladder infection after potential spinal cord injury, and after the S100A8 is perfused into the bladder, the colony count of the bladder tissue is obviously reduced, so that the S100A8 has an infection prevention effect; meanwhile, the damage repair capacity of the bladder epithelium is remarkably improved, the compound can be used as a prevention / treatment target spot for preventing bladder infection after spinal cord injury, and a new thought is provided for clinical prevention and treatment of bladder infection after spinal cord injury.

The application discloses a device and method for rapidly determining total number of bacteria based on multi-wavelength reflection spectrum, which comprises: a light emitting element for emitting a continuous light beam; a monochromator arranged in the emission direction of the light beam for separating the light beam into monochromatic light of different wavelengths; an integrating sphere for receiving the monochromatic light separated by the monochromator and converging the diffused monochromatic light; a constant temperature element arranged in the integrating sphere for keeping the standard sample at constant temperature, the diffused reflection being generated after the monochromatic light irradiates the constant temperature standard sample; a photoelectric conversion element connected with the integrating sphere for converting the optical signal into an electrical signal; and an oscilloscope connected with the photoelectric conversion element for reading, calculating and recording the electrical signal conducted by the photoelectric conversion element. The application solves the technical problem that the existing detection method has a large error in detection result due to the size of bacteria individuals, the state of the sample and the color difference, so as to achieve the purpose of rapidly and accurately determining the total number of bacteria.

The invention discloses a construction method of a growth prediction model of burkholderia gladioli in soaked black fungus and application of the constructed model, the method comprises the following steps: inoculating burkholderia gladioli containing rifampicin resistance into a soaked black fungus sample, putting the soaked black fungus sample into a plurality of incubators at different temperatures, and culturing; calculating the colony count of rifampicin-resistant burkholderia gladioli, and determining the growth curve of the burkholderia gladioli; respectively fitting the three first-level models and the two second-level models at different temperatures by utilizing statistical analysis software; by comparing comprehensive evaluation parameters, an optimal primary model and an optimal secondary model are selected, SGompertz is screened out as the optimal primary model, a quadratic polynomial model is screened out as the secondary model, the models are applied to prediction of the growth condition of burkholderia gladioli in soaked black fungus, the shelf life of the black fungus and the quantitative microbial risk can be predicted, and the quality guarantee period of the black fungus can be predicted. And the food quality can be improved.

This invention belongs to the field of biomedical technology, specifically relating to the application of a pharmaceutical composition in the fight against mycobacterial infections. The combination of juglottisol and bedaquiline significantly enhances the activity against mycobacterial infections. Compared to bedaquiline alone, 2 μg / ml of juglottisol combined with bedaquiline reduced the colony count of MmpL5-MmpS5 strains to varying degrees, from 1 / 16 MIC to 2 MIC of bedaquiline. Juglottisol, as an synergist of bedaquiline, enhances the anti-mycobacterial activity of bedaquiline. The minimum inhibitory concentration (MIC) of juglottisol against the Rv0678 mutant strain in vitro is 16 μg / ml, and the MIC of BDQ against the Rv0678 mutant strain in vitro is 1 μg / mL. The combination of 1 μg / ml zirconia thiazide and BDQ reduced the MIC of the Rv0678 mutant strain against BDQ from 1 μg / ml to 0.03 μg / ml (32-fold increase), which was lower than the MIC of the wild-type strain H37Rv (0.06 μg / ml), reversing BDQ resistance and further enhancing its activity against mycobacterial infections.

The utility model provides a microbial colony counting device, and relates to the technical field of microbial research. A supporting plate is slidably mounted on the inner side of the bottom of the incubator; a first motor is mounted on the left side of the incubator; a second lead screw is rotationally mounted on the inner side of the top of the incubator, and the second lead screw is in transmission connection with the first lead screw through a chain; a camera fixing block is installed on the outer side of the second lead screw. Two multi-section electric cylinders are fixed to the top of the supporting plate. A second motor is mounted at the tops of the two multi-section electric cylinders; a rotary supporting block is fixed to the top end of a main shaft of the second motor; five groups of culture dish brackets are arranged in a cavity in the inner side of the incubator at equal intervals; and a culture dish is placed at the top of each of the five groups of culture dish brackets. According to the device, the culture dish does not need to be taken out during counting, microbial spores are prevented from spreading, the environmental sanitation in a culture room is ensured, and the health of inspectors can be prevented from being affected.

The invention discloses a method and a kit for detecting the colony count of inactivated bacteria of lactobacillus paracasei K56 before inactivation, and relates to the technical field of instrument analysis. According to the method, at least one of citric acid, GPRPK peptide, GP (Hyp) GAG peptide, L-methionine, Asp-Leu peptide and Asp-Phe peptide is taken as a target substance, the colony count of inactivated bacteria of the lactobacillus paracasei K56 before inactivation is calculated by testing the content of the target substance, the detection accuracy is relatively high, a theoretical basis is provided for quantitative detection of postbiotics, and the method has a good application prospect. The establishment of industrial standards for the quantitative detection of the metastatic products is also facilitated.

To mitigate burden on a user regarding counting colonies.SOLUTION: A colony counting device includes: an acquisition section that acquires an image of colonies generated in a test individual; and an execution section that executes first software, the execution section executing processing of counting the number of the colonies from the image of the colonies. The execution section reads a user file that holds information in a matrix format, the user file being created by second software different from the first software, in order to count the colonies generated in the test individual or to manage a count result of the colonies. Furthermore, the execution section creates a count table to be used to count the colonies based on the user file.SELECTED DRAWING: Figure 18

1. Name of the Design Product: Total Colony Count Analyzer. 2. Use of the Design Product: For the automatic counting and analysis of microbial colonies (such as parameters like total coliforms, nitrifying bacteria, total colony count, etc.). 3. Design Key Points of the Design Product: Lies in the shape. 4. Picture or Photograph that Best Illustrates the Design Key Points: Stereogram. 5. The bottom surface of the Design Product is a part that is not easily seen or not seen during use, and the bottom view is omitted.

The invention belongs to the technical field of aquatic product preservation, and particularly relates to a preservation method for improving the storage quality of frozen fish, a frozen fish storage and preservation technology of plant extract, far infrared function packaging and magnetic field auxiliary freezing is established, a plant extract Pickin emulsion stabilized by soybean protein nanoparticles is adopted, the initial colony count is efficiently reduced, and the storage quality of the frozen fish is improved. Surface dirt is removed, and meanwhile antioxidant protection is achieved; a high-barrier packaging bag with a far-infrared composite function is adopted, physical protection and high barrier property are provided, activity of residual microorganisms is continuously inhibited through precise far-infrared radiation, metabolism of fish meat is slowed down, the cell state is improved, in the freezing process, magnetic field assistance is adopted, an electromagnetic field with specific strength is applied, the fish meat quickly penetrates through a maximum ice crystal generating zone, and freezing is achieved. And the ice crystals are fine and uniformly distributed, the mechanical damage of the ice crystals to fish cells is obviously reduced, so that the quality of frozen fish is improved, and the ice crystals have the advantages of no residue, long preservation time and the like, and have wide application prospects in the field of aquatic product preservation.

The invention discloses a large yellow croaker antibacterial polypeptide Lapuscin and application thereof. The amino acid sequence of the large yellow croaker antibacterial polypeptide Lapuscin is as shown in SEQ ID NO.01. The compound disclosed by the invention has strong antibacterial and antifungal activity, an excellent antibacterial effect and a high sterilization rate, for example, a sterilization kinetic curve shows that the colony count is obviously reduced within a short time. The pseudosciaena crocea strain is derived from pseudosciaena crocea, adapts to the aquatic product environment innate, is suitable for an aquatic product feed additive to prevent and control pathogenic bacteria such as vibrio and improve the culture efficiency, can be developed into an antibacterial agent and an antibacterial drug, and is used for wider infection treatment.

This invention relates to the field of microbial detection technology and discloses a method for detecting the colony content of cosmetics based on an intelligent sensing system. The method includes: projecting three-phase phase-shifted structured light onto a culture plate, demodulating to obtain the diffuse reflection alternating component matrix, and calculating the reduced scattering coefficient matrix to decouple static scattering interference; subsequently extracting the true microbial absorption coefficient matrix excluding powder interference, and calculating the speckle time contrast matrix in conjunction with continuously acquired speckle time series; exponentially fusing the two to construct a biochemical and physical activity fusion index matrix, and obtaining the cumulative biomass through local background smoothing and spatial integration; finally, adaptively generating a judgment threshold based on the global data of the culture plate, performing step-constraint screening to remove artifacts, and calculating the absolute total colony count. This invention effectively overcomes the problem of serious early false detections caused by cosmetic matrix artifacts, achieving accurate, closed-loop quantitative identification of live colonies without dependence on external sample libraries.

The invention relates to the related technical field of probiotic fermentation, in particular to an extraction process of a probiotic preparation for nasal cavity micro-ecological health care, and the extraction process comprises the following specific steps: step 1, raw material preparation: S1, selecting a mixture of bacillus subtilis and bacillus licheniformis as a probiotic strain, and the bacterial colony number is gt; the bacterial colony count ratio of the bacillus subtilis to the bacillus licheniformis is 1 to 1; s2, a fermentation medium is an LB broth medium and comprises 10 g / L of tryptone, 5 g / L of yeast extract, 10 g / L of NaCI, 20 g / L of glucose, 2 g / L of dipotassium phosphate, 2 g / L of diammonium hydrogen citrate, 10 g / L of sodium acetate, 0.28 g / L of magnesium sulfate, 0.58 g / L of manganese sulfate, 1 g / L of Tween 80 and 0.5 g / L of cysteine hydrochloride; according to the extraction process of the probiotic preparation for nasal cavity micro-ecological health care, by optimizing the steps of strain activation, seed solution expanding culture and fermentation culture, probiotics can be cultured more efficiently, so that the overall fermentation period is shortened.