15 results about "Colony counts" patented technology

The present invention belongs to the technical field of biopharmaceuticals and specifically relates to use of a pharmaceutical composition in combating mycobacterial infection. The use of zuclopenthixol in combination with bedaquiline exhibits significantly enhanced anti-mycobacterial infection activity. Compared to the use of bedaquiline alone, the use of 2 μg / ml zuclopenthixol in combination with bedaquiline results in varying reductions in colony counts of the MmpL5-MmpS5 strain when the concentration of bedaquiline ranges from 1 / 16 MIC to 2 MIC. As an enhancer of bedaquiline, zuclopenthixol can enhance the anti-mycobacterial infection activity of bedaquiline. The minimum inhibitory concentration of zuclopenthixol against the Rv0678 mutant strain in vitro is 16 μg / ml, and the minimum inhibitory concentration of BDQ against the Rv0678 mutant strain in vitro is 1 μg / mL. The use of 1 μg / ml zuclopenthixol in combination with BDQ reduces the MIC of BDQ against the Rv0678 mutant strain from 1 μg / ml to 0.03 μg / ml (a 32-fold increase in efficacy), which is lower than the MIC against the wild strain H37Rv (0.06 μg / ml), reversing resistance to BDQ and further enhancing the anti-mycobacterial infection activity.

A system for detecting a target bacteria is disclosed. The system comprises a flow cytometer. The flow cytometer is configured to receive a fluid sample, wherein the fluid sample comprises at least a target bacteria and at least a contaminant bacteria. The flow cytometer is also configured to generate a first enumeration of a total bacteria in the fluid sample during a pre-incubation phase. The fluid sample is then incubated during an incubation phase. The flow cytometer then generates a second enumeration of the total bacteria in the fluid sample during a post-incubation phase. A computing device then determines a growth ratio of the total bacteria as a function of the first enumeration and the second enumeration. Finally, the computing device identifies the presence of the at least a target bacteria as a function of the growth ratio.

The invention provides a high-purity bovine bone collagen peptide gel filtration chromatography purification method, and belongs to the technical field of bioactive peptide purification. The method comprises the following five core steps: pretreatment, gel column preparation, dynamic equilibrium, sample loading and elution, and post-treatment: firstly, carrying out ultrafiltration pretreatment on a bovine bone collagen peptide crude extract, then filling a chromatographic column with a specific diameter-height ratio by using cross-linked sephadex, carrying out dynamic equilibrium by using an accurately adapted equilibrium liquid after column efficiency verification, and finally, carrying out dynamic equilibrium by using a chromatographic column with a specific diameter-height ratio. The sample loading amount is dynamically matched through a formula, a gradient isothermal mode is adopted in the elution process, and finally a high-purity product is obtained through freeze drying. Through multi-step parameter collaborative optimization and precise regulation and control of a self-created formula, the problems of low purification efficiency, poor product purity and wide molecular weight distribution in the prior art are solved, the purity of the obtained bovine bone collagen peptide is greater than or equal to 98%, the proportion of peptide fragments with molecular weight distribution of 500-3000Da and 1000-2000Da is greater than or equal to 70%, the heavy metal content and the colony count both meet high-standard requirements, the yield is stabilized at 52% or above, and the method is suitable for industrial production. Good industrial application prospects are realized.

The invention relates to the technical field of bioassay, and discloses a total bacterial colony number detection assembly and a bioassay system.The total bacterial colony number detection assembly comprises a sample area, a diluent area, a preparation container area, a carrier area, an incubator, a conveying device and a bacterial colony counting device which are arranged on a platform. A space moving device is arranged on the platform, is connected with the grabbing mechanism and the pipette, and is suitable for driving the grabbing mechanism and the pipette to move in a space above the platform. The grabbing mechanism is suitable for grabbing objects, the pipette is suitable for sucking and discharging liquid, the conveying device is a linear module with a tray arranged on a moving part, the tray is suitable for placing a carrier, and the linear module can drive the tray and the carrier to move between a first position and a second position to enter and exit from the incubator. The total bacterial colony number detection assembly can replace manual work to carry out the partial detection process of the total bacterial colony number, and compared with the mode that the total bacterial colony number is detected completely manually, the working efficiency is higher, and the accuracy is better.

The invention discloses a water sample automatic culture and total bacterial count determination system and method, the system comprises a shell and a central control unit, the interior of the shell is divided into a to-be-detected sample area, a pretreatment area and a bacterial culture counting area through a lifting partition plate, and the central control unit is connected with the control end of the lifting partition plate; the system and the method can realize the automation of the whole process from pretreatment, dilution, inoculation, constant-temperature culture to colony counting of the water sample, improve the detection efficiency and the reliability of the result, and reduce the workload of personnel.

The invention discloses a method for determining the natural transformation efficiency of bacterial drug-resistant plasmids, and belongs to the field of detection technologies. Culturing the natural transformant bacterial liquid of the bacterial drug-resistant plasmid for different times to obtain bacterial liquid at different times; testing the fluorescence intensity and the number of transformed bacterial colonies corresponding to the bacterial liquid at different times, and establishing a first correlation curve between the fluorescence intensity and the number of transformed bacterial colonies; testing the absorbance and the total colony count of the bacterial liquid at different times, and establishing a second correlation curve between the absorbance and the total colony count; taking the to-be-detected bacterial liquid, measuring the fluorescence intensity and absorbance, calculating the number of transformed bacterial colonies of the to-be-detected bacterial liquid according to the fluorescence intensity of the to-be-detected bacterial liquid and the first correlation curve, and calculating the total number of bacterial colonies according to the absorbance of the to-be-detected bacterial liquid and the second correlation curve; and calculating the ratio of the number of transformant colonies / the total colony number to obtain the natural transformation efficiency of the bacterial drug-resistant plasmids. The problem that errors are easily generated by manual counting is solved.

This invention relates to the field of colony identification technology, specifically to a method, system, device, and medium for the fusion processing of static and dynamic colony counts. The method involves static identification, which is performed at least twice to obtain two static colony result images, followed by dynamic identification. Dynamic identification includes: acquiring two adjacent static colony identification results; preprocessing the colony data; and updating the previous dynamic identification result with the next dynamic identification result. This invention mainly combines static and dynamic identification of colony images. The dynamic identification step compares and analyzes the results of two adjacent static identifications, identifying covered colonies or other interfering factors such as impurities, and correcting subsequent dynamic identification. The beneficial effect is that it obtains a more accurate colony count, solving the problem of large counting errors in current technologies.

The invention provides a biogas slurry low-pressure sprinkling irrigation microbial aerosol concentration and particle size testing method and device. The method comprises the following steps: testing the concentration of microorganisms in diluted biogas slurry by adopting a plate coating method; three kinds of low-pressure spray heads are selected, and the mounting height of the spray heads is set to be 1.2 m; the method comprises the following steps: by taking a nozzle as a starting point, arranging microbial aerosol measuring points at intervals of 8m along the radial direction, and selecting the average breathing height 1.64 m of a human body as the collection height at each measuring point; 4, counting microbial colonies on the culture dish by adopting anaerobic fermentation biogas slurry; counting microbial colonies growing on the culture plate by using a colony counting instrument, and correcting the number of the colonies by using a positivity-hole method, so as to obtain the concentration of the air microbial aerosol and the particle size distribution characteristics of the air microbial aerosol. According to the invention, deep research on the dissipation mechanism of the microbial aerosol in the biogas slurry low-pressure sprinkling irrigation process is realized, and a scientific and effective method and system support are provided for parameter optimization and health risk prevention and control of a biogas slurry low-pressure sprinkling irrigation system.

The invention belongs to the technical field of biological medicine and molecular biology, and provides application of S100A8 in preparation of a medicine for preventing / treating bladder infection after spinal cord injury. The S100A8 can be used as a prevention target of bladder infection after potential spinal cord injury, and after the S100A8 is perfused into the bladder, the colony count of the bladder tissue is obviously reduced, so that the S100A8 has an infection prevention effect; meanwhile, the damage repair capacity of the bladder epithelium is remarkably improved, the compound can be used as a prevention / treatment target spot for preventing bladder infection after spinal cord injury, and a new thought is provided for clinical prevention and treatment of bladder infection after spinal cord injury.

The application discloses a device and method for rapidly determining total number of bacteria based on multi-wavelength reflection spectrum, which comprises: a light emitting element for emitting a continuous light beam; a monochromator arranged in the emission direction of the light beam for separating the light beam into monochromatic light of different wavelengths; an integrating sphere for receiving the monochromatic light separated by the monochromator and converging the diffused monochromatic light; a constant temperature element arranged in the integrating sphere for keeping the standard sample at constant temperature, the diffused reflection being generated after the monochromatic light irradiates the constant temperature standard sample; a photoelectric conversion element connected with the integrating sphere for converting the optical signal into an electrical signal; and an oscilloscope connected with the photoelectric conversion element for reading, calculating and recording the electrical signal conducted by the photoelectric conversion element. The application solves the technical problem that the existing detection method has a large error in detection result due to the size of bacteria individuals, the state of the sample and the color difference, so as to achieve the purpose of rapidly and accurately determining the total number of bacteria.

The utility model provides a microbial colony counting device, and relates to the technical field of microbial research. A supporting plate is slidably mounted on the inner side of the bottom of the incubator; a first motor is mounted on the left side of the incubator; a second lead screw is rotationally mounted on the inner side of the top of the incubator, and the second lead screw is in transmission connection with the first lead screw through a chain; a camera fixing block is installed on the outer side of the second lead screw. Two multi-section electric cylinders are fixed to the top of the supporting plate. A second motor is mounted at the tops of the two multi-section electric cylinders; a rotary supporting block is fixed to the top end of a main shaft of the second motor; five groups of culture dish brackets are arranged in a cavity in the inner side of the incubator at equal intervals; and a culture dish is placed at the top of each of the five groups of culture dish brackets. According to the device, the culture dish does not need to be taken out during counting, microbial spores are prevented from spreading, the environmental sanitation in a culture room is ensured, and the health of inspectors can be prevented from being affected.

This invention relates to the field of microbial detection technology and discloses a method for detecting the colony content of cosmetics based on an intelligent sensing system. The method includes: projecting three-phase phase-shifted structured light onto a culture plate, demodulating to obtain the diffuse reflection alternating component matrix, and calculating the reduced scattering coefficient matrix to decouple static scattering interference; subsequently extracting the true microbial absorption coefficient matrix excluding powder interference, and calculating the speckle time contrast matrix in conjunction with continuously acquired speckle time series; exponentially fusing the two to construct a biochemical and physical activity fusion index matrix, and obtaining the cumulative biomass through local background smoothing and spatial integration; finally, adaptively generating a judgment threshold based on the global data of the culture plate, performing step-constraint screening to remove artifacts, and calculating the absolute total colony count. This invention effectively overcomes the problem of serious early false detections caused by cosmetic matrix artifacts, achieving accurate, closed-loop quantitative identification of live colonies without dependence on external sample libraries.

The aim is to provide a colony counting device and method that enable more accurate judgment regarding the validity of counting results and can efficiently count colonies even in samples where spread colonies have occurred. [Solution] A colony counter 10 for counting colonies, which are aggregates of microorganisms, comprises: a camera 13 for capturing a sample image from a petri dish 1 in which microorganisms are cultured; and a control unit 17 that detects spread colony ICs from the sample image based on the number of pixels obtained by binarizing the sample image captured by the camera 13, and determines the validity of the count result from the ratio of the total pixel value of the detected spread colony ICs to the area of ​​the petri dish 1.

The invention belongs to the technical field of biological medicines, and discloses a mud moxibustion preparation for adjuvant therapy of joint functions of stroke rehabilitation patients and a preparation method of the mud moxibustion preparation. The mud moxibustion preparation is a qi-tonifying and blood-activating formula with astragalus membranaceus and ligusticum wallichii as the core, the pathological core of a qi deficiency and blood stasis type stroke rehabilitation patient is precisely met, and rehabilitation requirements are met from the source. According to the preparation method of the mud moxibustion preparation for adjuvant therapy of the joint function of the stroke rehabilitation patient, the crushing granularity is accurately controlled through a process end, the redundant process is simplified, and high efficiency of industrial preparation is achieved. The matrix is selected from auxiliary materials meeting the medical-grade safety standard, no adverse reaction is caused by skin irritation tests, the formed product is stable in texture, and the product is not prone to falling off when attached to the curved surface of the joint. The finished product compulsively passes a microbial limit test (the total number of bacteria is less than or equal to 100cfu / g, and the total number of mould and saccharomycetes is less than or equal to 10cfu / g), completely conforms to the safety specification of medical auxiliary materials, and can meet the safety requirement of a patient for long-term use according to a treatment course.