82 results about "Colony counts" patented technology

The invention discloses quantitative determination and drug allergy determination kits for helicobacter pylori viable bacteria and a determination method. The method adopts viable helicobacter pylori as a sample to be detected; the property that the viable helicobacter pylori can generate urease is used for rapidly and accurately reading the quantity of the helicobacter pylori by a helicobacter pylori colony counting standard method (CFU / ml, namely the bacterium individual quantity in each ml of bacterium liquid and a colony quantity standard curve); the detection result is accurate and sensitive and has high reliability; the counting difficulty of existing helicobacter pylori clinic microbiological identification, caused by difficult culture and complicated operation, is solved; the drug allergy determination kit and a preparation method, which are expanded by the invention, can be used for carrying out various drug allergy tests at the same time; clinicians can rapidly and conveniently screen suitable anti-helicobacter pylori medicines so that the time and the labor are saved and the cost is saved, so as to provide a beneficial technical solution for clinical scientific treatment.

The invention relates to an ecological microbial inoculum for remedying oil-polluted soils and a preparation method thereof. The ecological microbial inoculum for remedying oil-polluted soils is a mixed microflora of bacillus subtilis, pseudomonas fluorescens, berea trichosporon asahii and candida tropicalis, which are optimally mixed in a ratio of 1:1:1:1 basically. The invention has the advantages that: after the microbial inoculum is applied to the oil-polluted soils, a micro-ecological dominant microflora can be formed rapidly; and in case that the microbial inoculum in an amount which is 2 to 5 percent based on the weight of surface soils (cultivation layer: about 15 to 20cm) is applied to the oil-polluted soils, the oil removing rate is more than or equal to 75 percent (by using the methylene chloride refluxing extraction / gravimetric method) within three months; and the ecological microbial inoculum has a high effective colony count, a strong adaptability, and an obvious effect on remedying the ecological environments of oil-polluted soils.

The invention discloses a high-dietary-fiber bran and red jujube probiotics chewing tablet. The chewing tablet is prepared by pelleting, low-temperature drying, size stabilizing and tabletting of bran and red jujube fermented grain powder, a flavoring agent, a coagulator and a lubricant, wheat bran powder rich in dietary fiber and red jujube mud with full function and high sugar content are taken as raw materials, lactobacillus plantarum and leuconostoc mesenteroides subjected to domestication expanding culture are taken as leavening agents, and the chewing tablet is made through solid state fermentation, vacuum freeze drying and high-speed crushing. The high-dietary-fiber bran and red jujube probiotics chewing tablet has the advantages of easy obtain of the materials, low cost, few addition of supplementary materials, palatable flavor, high dietary fiber content and rich nutrient and intestinal probiotics of the lactobacillus plantarum and leuconostoc mesenteroides, and combined colony count is maintained at 8X107cfu / g above level within the product warranty period; the chewing tablet having good conditioning and healthcare functions on human constipation is a good healthcare food and can be chewed and eaten directly.

The invention relates to a method for exploring indicator bacteria by utilizing n-butyl alcohol oxidizing bacteria as oil-gas microorganism, comprising the following steps of: carrying out block process on a soil sample; coating the soil sample in the hydrocarbon oxidizing bacteria medium by utilizing a spread-plate method in an aseptic operating room; and putting the hydrocarbon oxidizing bacteria medium in a static incubator to cultivate the hydrocarbon oxidizing bacteria, wherein the n-butyl alcohol is used as the unique carbon and energy source of hydrocarbon oxidizing bacteria medium, the cultivating parameter is set as 30 DEG C, 5 days form a cultivating period, and the needed soil sample is air dried rapidly, then dissolved in distilled water and put in a shaking table to activate for use. The method has the advantages of shortening the cultivating period to 5 days, simplifying the whole experiment operating process, enhancing the safety, accurately obtaining the quantity of the needed objective colony by utilizing the medium with n-butyl alcohol as the unique carbon source, and enhancing the reliability of the colony count data through reducing the cultivating time.

The invention discloses a method for measuring the content of live spores in a fungal microbial pesticide quickly. The method comprises the following steps of: (1) adding 0.25 to 5 weight percent of sodium deoxycholate into a fungal culture medium, and preparing flat plates; (2) soaking fungal microbial pesticide samples in sterilization water containing Tween 20 or polyethylene glycol octylphenol ether, and shaking; (3) diluting in a multiple gradient mode, coating the samples of different dilutability on the flat plate respectively and uniformly, and culturing at room temperature for 48 to 120 hours; (4) counting colony counts, wherein the flat plates on which the colony counts are 30 to 300 are effectively-counted flat plates; and (5) counting the content of the live spores, namely C is equal to T*N*10, wherein in a formula, C is the content of the fungal pesticide live spores, the unit is colony forming unit (CFU) / gram, T is dilution times corresponding to the counted flat plates, and N is average colony counts on a plurality of effectively-counted flat plates of the same dilution times. By the method, the content of the live spores of the fungal microbial pesticide can be measured simply, conveniently and quickly, and the detection accuracy is high.

The invention discloses a preparation method and application of bacillus subtilis for feed. The bacillus subtilis is subjected to high-density liquid-state fermentation and then treated, a protective agent is added, and freeze drying is performed to prepare a microbial inoculum serving as a feed additive. In the fermentation process of the strain, the inoculum size of a seed solution is 2-10% (V / V), the strain is inoculated into a fermentation culture medium, the temperature is 25-42 DEG C, fermentation is carried out for 20-48 hours, and the colony count is 5.8*10<10> cfu / g. The fermentation culture medium comprises molasses, corn flour, bean dregs and the like, so that the production cost is remarkably reduced. Compared with a traditional fermentation technology, the colony count of fermentation production is obviously increased, the fermentation period is short, and safety and environment friendliness are achieved; meanwhile, coarse raw materials such as the bean dregs and the molasses are adopted for fermentation production; the bacillus subtilis can be used as the feed additive and has the obvious effects of improving the intestinal environment of livestock and poultry, enhancing animal immunity and promoting animal growth; and the production wastes such as the bean dregs are turned into wealth, so that the recycling of waste resources is realized, the pollution of the waste materials to the environment is reduced, and the production cost is greatly reduced.

The invention provides application of TCC to prevention of colony diffuse growth in colony count determination. The method includes: taking Bacillus cereus, Escherichia coli and Staphylococcus aureusof appropriate dilution, adding 0.01%, 0.05%, 0.08%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.8%, and 1.0% TTC aqueous solution plate count agar, at the same time taking count agar without TTC as control, andobserving the growth condition of bacteria. In colony count determination, if a sample possibly contains Bacillus colonies growing diffusely on an agar medium surface, TTC with a concentration of 0.2%-0.3% can be added to the agar to prevent incalculability situation caused by diffusion of colonies.

The invention discloses a method for decontaminating dehydrated garlic products. The method for manufacturing the dehydrated garlic products includes steps of soaking and disinfecting selected garlicin acidic electrolytic water with the pH (potential of hydrogen) value of 2-4 for 5-15 min and then cleaning the garlic by the aid of pure water; placing the garlic in ozone water, carrying out cooling treatment on the garlic for 5-10 minutes and splitting the garlic; secondarily treating split raw materials by the aid of ozone water for 20-40 minutes; draining raw materials treated by the aid ofthe ozone water, then carrying out stepped temperature control drying on the raw materials, in other words, drying the raw materials at the temperatures of 110-120 DEG C for 1-2 hours, gradually reducing the temperatures until the temperatures reach 60-80 DEG C, and continuing to dry the raw materials until the moisture contents of the raw materials are reduced and reach 5-8%. The method has the advantages that the quantity of germs carried by the garlic can be reduced by 60%-67%, late sterilization can be omitted, the sterilization intensity of the method is far lower than the sterilization intensity of conventional processing technologies, dehydrated garlic which is subjected to contamination treatment by the aid of the method is little in color and nutritional component change, but thetotal colony count of the dehydrated garlic can be obviously reduced and reaches high standards, and the problem of dull sale due to the fact that the total colony count of existing garlic products exceeds standards can be effectively solved by the aid of the method.

The invention discloses a colony count alarming device for vessel ballast water, and relates to the technical field of ocean vessel transportation and offshore oil engineering. The alarming device comprises a base, a sample storage tank, a pressure detection unit, a turbidity detection unit, a fluorescence self-illumination detection unit and a comprehensive alarming unit, wherein the pressure detection unit, the turbidity detection unit and the fluorescence self-illumination detection unit are detection units and detect concentration of colony counts in the ballast water through energy detection of aerobic bacteria utilizing oxygen, ballast water turbidity detection and biological self-illumination detection respectively, and the comprehensive alarming unit is used for collecting and processing signals of the three detection units, and two of the three signals are output in an acoustic-optical manner for alarming. The overall device is high in redundancy and high in reliability and has the characteristics of compact size, convenience in detection, economical efficiency and practicability.

The invention discloses a broad-bean chili sauce maturity-promotion and aroma-enhancement fermentation bacterium agent and a preparation method thereof. The preparation method comprises the following steps: performing propagation culture on bacillus pumilus, lactobacillus fermentum, lactobacillus bifermentans, saccharomyces rouxii and aspergillus oryzae; mixing, inoculating to a sterilization matrix carrier culture medium by 4%-8%, and culturing at 32 DEG C for 48 hours, wherein the colony count is 108-1,010cfu / mL; after the fermentation is finished, adding 6%-10% of sucrose, 1%-5% of gelatin and 2%-5% of glycerin as heat-resistant protectants, flatly laying on a thin layer, performing far-infrared drying at 50-60 DEG C for 2-3 hours, and packaging in vacuum. According to the fermentation bacterium agent, the maturity promotion, aroma enhancement and fermentation of broad-bean chili sauce are enhanced and the quality of the broad-bean chili sauce is stabilized and improved.