Preparation method of high-activity microencapsulated lactic acid bacteria starter

Abstract

The invention discloses a preparation method of a high-activity microencapsulated lactic acid bacteria starter. The preparation method comprises the following steps: inoculating lactic acid bacteria to a culture medium to be cultured; filtering the bacterial liquid with a hollow fiber membrane, and concentrating to form a concentrated bacterial liquid; injecting a fresh culture medium into the concentrated bacterial liquid to be cultured; filtering the prepared bacterial liquid with the hollow fiber membrane and concentrating for 2-3 times to obtain a concentrated bacterial liquid with a unit colony count up to 1,011 cfu / ml above; adding gelatin, sucrose and whey protein into the concentrated bacterial liquid as wall materials; carrying out spray drying to obtain the microencapsulated lactic acid bacteria starter. According to the preparation method disclosed by the invention, the hollow fiber membrane is combined with a bioreactor to carry out high-density culture and concentration of the lactic acid bacteria, so as to implement online continuous filtration, concentration and culture and avoid pollution due to thallus transfer; the coupled spray drying at a low temperature is carried out to effectively protect the concentrated high-density bacteria, so as to obtain the lactic acid bacteria starter with a unit viable count up to 1*10<11>-1*10<12> cfu / ml.

Description

technical field [0001] The invention belongs to the field of food, and relates to a method for preparing a high-activity microencapsulated lactic acid bacteria starter, in particular to a method for preparing a high-activity lactic acid bacteria starter by combining hollow fiber concentration culture with low-temperature spraying. Background technique [0002] Lactic acid bacteria are the most commonly used starter for fermented foods and are currently the most widely used starter commercially. Traditional liquid starters require multiple activations and expansions. The process is cumbersome, and it is easy to increase the chance of phage contamination, which cannot meet the production needs. Direct drop starters are gradually replacing traditional liquid starters. [0003] High-density cultivation and enrichment and concentration of bacteria are important links in the preparation process of direct-injection fermentation inoculum, and the number of viable bacteria per unit r...

AI Technical Summary

Problems solved by technology

At present, the drying method of direct throwing starter mainly adopts spray drying and freeze drying. The commonly used method of spray drying is high temperature spray drying. Due to the high outlet temperature, some strains with poor heat resistance will be damaged in spray drying, while low temperature freezing Due to the low temperature of the drying method, low temperature stress is caused to the strain, which also reduces the vigor of the concentrated high-density bacteria, which seriously affects the viability of the unit bacteria of the direct-into-type starter, and cannot meet the production needs of the direct-into-type starter

[0005] The preparation process of direct-throwing starter has been reported so far, but there is no relevant report on the preparation of direct-throwing starter by hollow fiber concentration coupled with low-temperature spray drying technology

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