7results about How to "Keep active" patented technology

This invention discloses a mouse fixation device for multi-site acupuncture experiments, belonging to the field of mouse acupuncture point experiments. It consists of a support base, a telescopic support rod, and a mouse fixation assembly, which is movably connected to the support rod. The mouse fixation assembly includes a bracket and upper and lower fixation plates, which are adjustable in multiple dimensions via first and second self-locking rotating shafts. The upper and lower fixation plates close with an interference fit between fitting bosses and fitting openings. The central operating port is equipped with a rigid rubber adjustable fixing grid, which, along with a lever, can avoid acupuncture points. The lower fixation plate grid has adhesive straps corresponding to the mouse's limbs and head, with pre-reserved gaps between the plates. A suction cup is provided at the bottom of the support base to stabilize the device. This device achieves double secure fixation of the mouse, with flexible and adjustable operating angles, avoiding acupuncture point obstruction, protecting the mouse from mechanical damage, improving the accuracy and efficiency of acupuncture experiments, and adapting to the needs of multi-site acupuncture experiments on mice.

The invention provides a disinfection method of a ginseng explant, which comprises the following steps: soaking and disinfecting the ginseng explant by using a chemical disinfectant, soaking the soaked and disinfected explant into a first ethanol solution, taking out, igniting residual ethanol on the surface of the explant, burning, and cooling. According to the method disclosed by the invention, conventional chemical disinfection and high-concentration ethanol infiltration-flame burning are creatively combined, and the step-by-step and synergistic sterilization strategy is particularly suitable for explants with complicated structures, such as ginseng roots. Compared with a traditional disinfection method needing long-time soaking and multiple times of rinsing, the method has the advantages that the total disinfection time is greatly shortened and the explant preparation efficiency is remarkably improved by introducing an instantaneous firing step, and the explant preparation efficiency is greatly improved by reducing the total contact time of a chemical disinfectant, inactivating surface polyphenol oxidase by utilizing instantaneous high temperature and combining rapid cooling. The browning reaction is effectively inhibited, and the activity of the explant is ensured.

The present application relates to the technical field of biological nanomaterials, in particular to a multi-level structure calcium phosphate nanomaterial, a preparation method and application thereof.The present application prepares small-scale calcium phosphate nanoparticles through a biomimetic mineralization strategy, and then crosslinks the same with L-lysine or cystamine dihydrochloride through an EDC / NHS-mediated amide coupling method to form a size-controllable multi-level structure aggregate.Compared with the small-scale calcium phosphate nanoparticles, the multi-level structure, as a doping matrix of metal ions such as Cu and Mn, significantly improves the catalytic efficiency of the metal ions, as a drug carrier, improves the loading capacity of the drug, and the multi-level structure shows a higher tumor accumulation effect.The present application has a simple preparation method, can realize the size regulation of the multi-level structure calcium phosphate nanomaterial, and can realize the tumor microenvironment-responsive disintegration of the multi-level structure.The method has good process repeatability and high batch stability, is suitable for developing high-performance nanodrugs, and has good application potential in the biomedical field.

The application discloses a kind of functionalized magnetic beads, which are magnetic microspheres formed by wrapping magnetic beads with bifunctional nucleic acid, and can specifically bind mRNA.A fungal mRNA extraction kit composed of functionalized magnetic beads includes grinding material, lysing solution I, lysing solution II, magnetic bead binding solution, washing solution I, washing solution II and eluent.A mRNA extraction method includes sequentially adding grinding material, lysing solution I, lysing solution II, magnetic bead binding solution, washing solution I, washing solution II and eluent after sample pretreatment to obtain mRNA product.The application breaks through the traditional mRNA extraction method, obtains high-quality mRNA, and has the advantages of simple operation, safety, high sensitivity, good reproducibility and low cost.The mRNA obtained by the application can provide high-quality templates for RT-PCR detection, improve the specificity and sensitivity of detection, and has wide application prospects in the fields of clinical diagnosis of diseases, judicial identification, food safety detection, microbial detection and molecular biology.

The invention provides a method for separating human umbilical cord mesenchymal stem cells. The umbilical cord mesenchymal stem cells are obtained by sequentially carrying out umbilical cord tissue treatment, umbilical cord sheet treatment and umbilical cord block culture and separation. The method disclosed by the invention is simple to operate, the separation efficiency of the umbilical cord tissue is improved, the purity of the obtained cells is higher and is obviously superior to the requirement of the ISCT lowest identification standard, the production cost can be effectively reduced, and industrial application is facilitated.

The application belongs to the technical field of biological medicine, and particularly relates to a thymus tissue dissociation reagent, a kit and application thereof in obtaining thymus epithelial cells. The thymus tissue dissociation reagent at least comprises collagenase D, dispersinase II, deoxyribonuclease I, dissociation enzyme and a dissolving reagent; and collagenase II and / or collagenase IV and / or trypsin. The cell survival rate of the thymus tissue dissociation reagent after dissociating the thymus tissue is maintained at a high level of 95.7% to 99.6%, which is significantly higher than that of the prior art; and after sorting, the number of thymus epithelial cells enriched and obtained from each gram of thymus tissue is 1.38e 5 ~1.06e 6 , and the success rate of enriching and obtaining thymus epithelial cells is as high as 95%, which is significantly higher than that of the prior art; and the proportion of epithelial stem cells in the thymus epithelial cells is 65.7%, which is significantly higher than that of the prior art.

The invention discloses a synthesis method of porous polylactic acid microspheres, and belongs to the field of biomedical materials. The method comprises the following steps: preparing a first water phase taking a water-soluble polymer as a pore-foaming agent, and dispersing the first water phase in a polylactic acid organic solution to form a water-in-oil-in-water primary emulsion; introducing the primary emulsion into a curing bath containing a poor solvent, so that the organic solvent is extracted, the polylactic acid is cured, and microspheres wrapping pore-foaming agent liquid drops are formed; and finally, washing to remove the pore-foaming agent to form a porous structure. The core of the method is that the internal water-phase liquid drops are used as a sacrificial pore-forming template, and the microspheres are cured by a solvent extraction method. The method is mild in condition, green and environment-friendly, the aperture, porosity, particle size and pore connectivity of the microspheres can be independently and accurately regulated and controlled by adjusting parameters such as emulsification conditions and phase proportion, and the prepared microspheres have the characteristics of open pores and high connectivity and have excellent application prospects in the fields of drug sustained release and tissue engineering.