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An inverted microwell (102) provides rapid and efficient microanalysis system (100) and method for screening of biological particles (128), particularly functional analysis of cells on a single cell basis. The use of an inverted open microwell system (102) permits identification of particles, cells, and biomolecules that may be combined to produce a desired functional effect also functional screening of secreted antibody therapeutic activity as well as the potential to recover cells and fluid, and optionally expand cells, such as antibody secreting cells, within the same microwell.

The invention relates to a preparation method and application of electrospinning nanofiber. The preparation method is as follows: synthetic and/or natural polymer materials are dissolved in a volatilesolvent under normal temperature and normal pressure, functional substances are added, and nanofibers are obtained by electrospinning; the functional substances comprise, but are not limited to an odor substance, an organic fluorescent compound, a pigment, a temperature sensitive luminescent and color changing material, a cell growth factor, a hemostatic substance, one or a plurality of nutrientsincluding hyaluronic acid, collagen, and the like which promote skin growth. The nanofiber made by electrospinning has the special effects of sterilization, aromatic odor, fluorescent luminescence, multiple colors, temperature sensitive luminescence and color-changing, promoting wound healing, promoting inhibition and interfering with body function, maintaining skin cell vitality and the like. The method can be used in air purification, automotive air purification, fresh air systems, medical, beauty care, and other nanofiber products.

The invention discloses a method for efficiently separating single antigen-specific B lymphocyte from spleen cells. High-throughput rapid screening of single antigen-specific B lymphocyte is realizedby negative screening adopting multiple lymphocyte surface marker antibodies, establishment of a rapid fluorescence marked antigen substance method and high-specificity screening by use of a fluorescence marked antigen substance, and the positive rate of the finally obtained antibody-specific B lymphocyte is increased from less than 5% in a traditional hybridoma method to 30%. Therefore, the scheme can shorten monoclonal antibody development time to a great extent and greatly increase the number and diversity of obtained monoclonal antibodies.

The present invention is directed to a device for minimizing or preventing damages due to ischemia that can occur within supported or retracted dermal and/or subdermal living tissue, most particularly during surgical procedures, by one or a combination of several means including cyclically applying and reducing supporting or retracting pressure at each of at least two tissue sections into which the supported or retracted tissue is subdivided, bathing these tissue sections with gases and/or liquids such as oxygen and oxygenated blood, presenting low-pressure regions or partial vacuum to areas within these tissue-sections to stimulate bleeding to encourage blood perfusion, controlling the temperature of these tissue sections to forestall ischemic damage, and mechanically moving at least a portion of these tissue sections to stimulate blood perfusion with, for example, a vibrating mechanism.

The present invention provides a biocompatible prepolymer comprising hydrophilic and hydrophobic segments, wherein the hydrophobic segments have at least one ethylenically unsaturated functional group and at least 5% of the segments have two or more ethylenically unsaturated functional groups and water. The invention further provides a biocompatible prepolymer composition comprising hydrophilic and hydrophobic prepolymers, wherein at least one of the hydrophobic prepolymers has at least one ethylenically unsaturated functional group and at least 5% of the prepolymers have two or more ethylenically unsaturated functional groups and water. The invention further provides use of the prepolymer or prepolymer compositions of the invention in biomedical applications such as tissue engineering, as bone substitutes or scaffolds, and in wound treatment.

An oil shale rotary kiln dry distillation and circulating fluidized bed combustion process makes organic combination of a rotary kiln dry distillation with a circulating fluidized bed; oil shale particles are mixed with thermal-cycle ash from a circulating fluidized bed boiler and part of circulating fluidized bed boiler bottom ash, and then are sent into a rotary kiln for dry distillation to prepare shale oil and dry distillation coal gas; the dry distillation coal gas has a high calorific value; a part of the coal gas is used as recycle wind of a semicoke material recycle device, and enters the circulating fluidized bed boiler with oil shale semicoke particles for combustion, and the residual part is delivered into an internal combustion engine for combustion generation. The invention can increase shale oil and coal gas quality, reduce energy loss and environment pollution, and improve the comprehensive utilization degree.

The invention discloses a recombinant adeno-associated virus-NADH dehydrogenase sigmasubunit 4 gene total length for treating Leber hereditary optic neuropathy and a medicament. The total length of the gene is shown in a nucleotide sequence SEQ ID NO:1, wherein the nucleotide sequence, consisting of a CAG starter sequence, a coded sequence of ND4 of a mitochondrial localization sequence with Cox10 and UTR with the length of 625bp, has the total length of 3824bp. The medicament is injected into an eye vitreous chamber and is used for treating Leber hereditary optic neuropathy; because the medicament can be injected into the vitreous chamber, the activity can be kept in the vitreous chamber, and the medicament can be effectively subjected to transfection to optic nerve cells; signal peptide at the front end of the protein N directionally guides the protein to enter mitochondria, and the mature protein ND4 enters the mitochondria to play a function; therefore, the Leber hereditary optic neuropathy can be effectively treated by using the medicine.

Said invention provides equality size and stable dispersion chitosan microsphere and microcapsule medicine carrier to protein, polypeptide, anticancer agent, hormone etc hydrophilicity medicine and oil soluble medicine for releasing control and targeting administration. The characteristics are that, 1, equality size and stable dispersion chitosan microsphere and microcapsule medicine carrier is made by dissolving hydrophilicity medicine in aqueous solution of acetic acid, pressuring said water phase into oil phase through microporous membrane to get equality size emulsion drop, 2, dissolving oil soluble medicine in oil solvent and hydrophilicity medicine in aqueous solution of acetic acid, preparing O#-[1]/W colostrum by homogeneous phase emulsifier, pressuring it into oil phase through microporous membrane to obtain equality size composite emulsion drop, solidifying it to obtain equality size and stable dispersion chitosan microsphere and microcapsule medicine carrie. Under optimized condition, the diameter distribution coefficient of microsphere and microcapsule are controlled within 11 % , the diameter are controlled freely in 1-100 micrometer.

The invention relates to a method for transplanting cotton seedlings. The method comprises three steps of preparation of a matrix, sowing and transplanting sequentially. Step 1, cotton stalks, rape stalks, corn stalks, soybean stalks or wheat straws are crushed, added with a nitrogenous fertilizer, and mixed with human excrement or livestock and poultry manure bio-fertilizer or other fertilizers to obtain a mixture which is treated to obtain a prepared material, then the prepared material is sun-dried or baked, added with a fungicide and bagged for spare use; step 2, the prepared cotton stalk matrix or the matrixes prepared from other stalks are filled in a seedling raising aperture disk or flatly spread on a plastic film laid on the ground as a seedbed, seeds are sown, watered and covered with an agricultural film, and seedlings are raised under heat insulation condition; step 3, an antitranspirant and a rooting agent are applied before transplanting; the taken seedlings are bound, put in the watered rooting agent solution for root soaking, and then taken out and put in a watered gel containing a water retention agent to dip the roots; when in transplantation, the water retention agent is applied to planting furrows; after transplantation, the seedlings are covered with soil, and irrigated with adequate water for fixing the roots. The method is characterized by high seedling survival rate, stable effect, short rejuvenation period, low cost and environmental protection.

The invention discloses a production method for an organic fertilizer. The production method can be used for mixing excrements of herbivore animals and plasters or plant ashes and coal ashes which are obtained by combusting lime sludge of a sugar factory or manioc waste and bagasse of a starch factory and quickly converting organic waste into available organic fertilizers by a zymophyte ecological method. According to the method provided by the invention, the stack fertilizer preparing time can be shortened, and the labor and the time can be saved. By using the organic fertilizer produced by the method, a perfect root environment can be constructed, so that rot diseases can be avoided; plants can fully realize the functions of absorbing the fertilizers, and crops can quickly grow up; the physical property and the chemical property as well as the microbial state of the soil are thoroughly balanced, so that the soil can be forever kept in an optical state for culturing crops. The organic fertilizer produced by the invention can be mixed with other trace elements, a plant growth promoter, chemical fertilizers and the like to prepare a composite fertilizer. Beneficial microflora can be added for further treatment; the non-toxic and harmless microorganism fertilizer can be compounded.

The invention provides a method for improving the sensitivity and linearity of a latex reagent. An adopted detection kit comprises a reagent R1 and a reagent R2. The method is characterized in that the latex reagent is a latex reagent mixed with latexes with large and small particle sizes, and is processed to prepare a C-creative protein latex turbidimetric detection kit, and the kit comprises the reagent R1 and the reagent R2. The method comprises the following steps: (1) directionally coating latex microspheres with large particle sizes with mouse to-be-tested antigen monoclonal antibodies; (2) directionally coating latex microspheres with small particle sizes with rabbit or sheep to-be-tested antigen monoclonal antibodies; (3) mixing the coated latex microspheres with the large particle sizes and the coated latex microspheres with the small particle sizes.

The invention belongs to the technical field of artificial pollination of fruit trees, specifically relates to a pollen suspension liquid for liquid pollination of fruit trees, and a preparation method thereof. The pollen suspension liquid mainly comprises the following components, by weight, 0.005-0.02% of boric acid, 0.005-0.01% of calcium nitrate, 0.01-0.02% of sodium carboxymethyl cellulose, 0.2-0.4% of pollen, and the balance of water. The pollen suspension liquid provided by the present invention can be placed for a long time, and can be prepared into a concentrated solution. In addition, the pollen suspension liquid provided by the present invention is easy to use, and can be directly sprayed on pistillate flower in a flowering stage to carry out the artificial liquid pollination, such that the fruit set percentage of the fruit trees can be more than 48%.

A magnetic separation system configured to separate with high qualitative and quantitative yield magnetized cells from cell mixtures, comprising at least one electromagnet structured to generate a magnetic field flux about a plurality of separation zones and sufficient to attract a majority of the magnetized cells in the mixture, and a pump to drive the cell mixture at a controlled flow rate through a tube disposed within the zones thereby separating a majority of the magnetized cells from the mixture. The system is particularly useful to retrieve rare cells from a fluid mixture of cells having low abundance of the rare cells relative to the rest of the cells while sustaining viability of the cells.

The invention provides umbilical cord mesenchymal stem cell injection as well as a preparation method and application thereof. The umbilical cord mesenchymal stem cell injection comprises mesenchymal stem cells and a frozen stock solution, wherein the frozen stock solution comprises the following components: a balanced electrolyte solution with the volume percentage of 25-70 percent, hydroxyethyl starch 130/0.4 with the mass/volume percentage (g/ml) of 1-10 percent, triphosadenine disodium magnesium chloride with the volume percentage of 5-20 percent, clinical-grade DMSO with the volume percentage of 5-20% and 20% human albumin with the volume percentage of 1-50 percent. The prepared umbilical cord mesenchymal stem cell injection is free of animal serum, safe, high in cell viability after cryopreservation resuscitation, can be used for treating inflammatory bowel disease, has a good effect and has no toxic or side effects.

The invention relates to umbilical cord mesenchymal stem cell injection and a preparation method and application thereof. The injection comprises umbilical cord mesenchymal stem cells and mixed solution; the mixed solution comprises balanced electrolyte solution, hydroxyethyl starch, adenosine disodium triphosphate-magnesium chloride, dimethyl sulfoxide and human serum albumin. The preparation method comprises the following steps: (1) fully and evenly mixing the balanced electrolyte solution, the hydroxyethyl starch and the human serum albumin, and performing filtration sterilization; (2) adding the umbilical cord mesenchymal stem cells; (3) adding the dimethyl sulfoxide; (4) performing split charging and frozen preservation to finish preparation; the invention also provides the application of the injection in the aspect of drugs for treating chronic ischemic heart diseases. The injection disclosed by the invention has a very good resuscitation effect after the frozen preservation, can be directly injected after being unfrozen and resuscitated, and has a remarkable effect on treatment on the chronic ischemic heart diseases.

The invention discloses a grafting method taking masson pine tissue culture seedlings as stocks. The grafting method comprises processes of stock cultivation, scion preparation, seedling grafting and post-grafting management and protection; by taking the masson pine tissue culture seedlings cultivated for 4 to 6 months as the stocks and collecting bud sticks germinating on stock plants as scions, grafting is performed by adopting a split grafting method in December to January or in March to April; grafted seedlings are cultivated under the conditions of suitable light and humidity, and water and fertilizer management and pest control are performed by a conventional method. According to the grafting method disclosed by the invention, the masson pine tissue culture seedlings with developed roots, vigorous metabolism and rapid growth are cultivated through a tissue culture technological means to serve as the stocks, therefore not only can the seedling period of the stocks be shortened and the grafting survival percentage be increased but also the genetic gain of an excellent variety is ensured; adverse effects brought by the fact that heterogeneous-stock grafting is performed by taking slash pines as the stocks widely in masson pine grafting in production at present are effectively avoided, and the working effect of masson pine tissue culture stocks is greatly promoted; the grafting method has a broad application prospect, and has better economic benefit, social benefit and ecological benefit.

The invention belongs to the field of a rice seed embryo vigor retention technology, and discloses a natural plant extract rice seed embryo vigor retention agent as well as a preparation method and an application thereof. The rice seed embryo vigor retention agent comprises the following components by weight percent: 49.50-50.00% of a cany tea extract, 25.10-25.50% of a tea extract, 24.50-25.00% of a mulberry leaf extract, 0.15-0.20% of pericarpium citri reticulatae essential oil and 0.20-0.25% of clove essential oil. The natural plant extract rice seed embryo vigor retention agent is reasonable in formulation, fully utilizes the characteristics of all the components, and the components can be reasonably combined and matched, so that the comprehensive bactericidal effect can be achieved; the experiments proves that the natural plant extract rice seed embryo vigor retention agent is very obvious in the sterilizing effect on rice seeds, and effectively inhibits the adverse effect of microbial activities on the seed vigor; the natural plant extract rice seed embryo vigor retention agent achieves the aims of being safe, environment-friendly, practical and effective in the aspect of maintaining the seed vigor; and the natural plant extract rice seed embryo vigor retention agent is easy to process and prepare and convenient to use, and an ideal medicine is provided for maintaining the seed vigor and prolonging the storage period.