4results about How to "Keep active" patented technology

This invention discloses a mouse fixation device for multi-site acupuncture experiments, belonging to the field of mouse acupuncture point experiments. It consists of a support base, a telescopic support rod, and a mouse fixation assembly, which is movably connected to the support rod. The mouse fixation assembly includes a bracket and upper and lower fixation plates, which are adjustable in multiple dimensions via first and second self-locking rotating shafts. The upper and lower fixation plates close with an interference fit between fitting bosses and fitting openings. The central operating port is equipped with a rigid rubber adjustable fixing grid, which, along with a lever, can avoid acupuncture points. The lower fixation plate grid has adhesive straps corresponding to the mouse's limbs and head, with pre-reserved gaps between the plates. A suction cup is provided at the bottom of the support base to stabilize the device. This device achieves double secure fixation of the mouse, with flexible and adjustable operating angles, avoiding acupuncture point obstruction, protecting the mouse from mechanical damage, improving the accuracy and efficiency of acupuncture experiments, and adapting to the needs of multi-site acupuncture experiments on mice.

The invention provides a disinfection method of a ginseng explant, which comprises the following steps: soaking and disinfecting the ginseng explant by using a chemical disinfectant, soaking the soaked and disinfected explant into a first ethanol solution, taking out, igniting residual ethanol on the surface of the explant, burning, and cooling. According to the method disclosed by the invention, conventional chemical disinfection and high-concentration ethanol infiltration-flame burning are creatively combined, and the step-by-step and synergistic sterilization strategy is particularly suitable for explants with complicated structures, such as ginseng roots. Compared with a traditional disinfection method needing long-time soaking and multiple times of rinsing, the method has the advantages that the total disinfection time is greatly shortened and the explant preparation efficiency is remarkably improved by introducing an instantaneous firing step, and the explant preparation efficiency is greatly improved by reducing the total contact time of a chemical disinfectant, inactivating surface polyphenol oxidase by utilizing instantaneous high temperature and combining rapid cooling. The browning reaction is effectively inhibited, and the activity of the explant is ensured.

The present application relates to the technical field of biological nanomaterials, in particular to a multi-level structure calcium phosphate nanomaterial, a preparation method and application thereof.The present application prepares small-scale calcium phosphate nanoparticles through a biomimetic mineralization strategy, and then crosslinks the same with L-lysine or cystamine dihydrochloride through an EDC / NHS-mediated amide coupling method to form a size-controllable multi-level structure aggregate.Compared with the small-scale calcium phosphate nanoparticles, the multi-level structure, as a doping matrix of metal ions such as Cu and Mn, significantly improves the catalytic efficiency of the metal ions, as a drug carrier, improves the loading capacity of the drug, and the multi-level structure shows a higher tumor accumulation effect.The present application has a simple preparation method, can realize the size regulation of the multi-level structure calcium phosphate nanomaterial, and can realize the tumor microenvironment-responsive disintegration of the multi-level structure.The method has good process repeatability and high batch stability, is suitable for developing high-performance nanodrugs, and has good application potential in the biomedical field.

The application belongs to the technical field of biological medicine, and particularly relates to a thymus tissue dissociation reagent, a kit and application thereof in obtaining thymus epithelial cells. The thymus tissue dissociation reagent at least comprises collagenase D, dispersinase II, deoxyribonuclease I, dissociation enzyme and a dissolving reagent; and collagenase II and / or collagenase IV and / or trypsin. The cell survival rate of the thymus tissue dissociation reagent after dissociating the thymus tissue is maintained at a high level of 95.7% to 99.6%, which is significantly higher than that of the prior art; and after sorting, the number of thymus epithelial cells enriched and obtained from each gram of thymus tissue is 1.38e 5 ~1.06e 6 , and the success rate of enriching and obtaining thymus epithelial cells is as high as 95%, which is significantly higher than that of the prior art; and the proportion of epithelial stem cells in the thymus epithelial cells is 65.7%, which is significantly higher than that of the prior art.