22results about How to "Keep active" patented technology

This invention discloses a mouse fixation device for multi-site acupuncture experiments, belonging to the field of mouse acupuncture point experiments. It consists of a support base, a telescopic support rod, and a mouse fixation assembly, which is movably connected to the support rod. The mouse fixation assembly includes a bracket and upper and lower fixation plates, which are adjustable in multiple dimensions via first and second self-locking rotating shafts. The upper and lower fixation plates close with an interference fit between fitting bosses and fitting openings. The central operating port is equipped with a rigid rubber adjustable fixing grid, which, along with a lever, can avoid acupuncture points. The lower fixation plate grid has adhesive straps corresponding to the mouse's limbs and head, with pre-reserved gaps between the plates. A suction cup is provided at the bottom of the support base to stabilize the device. This device achieves double secure fixation of the mouse, with flexible and adjustable operating angles, avoiding acupuncture point obstruction, protecting the mouse from mechanical damage, improving the accuracy and efficiency of acupuncture experiments, and adapting to the needs of multi-site acupuncture experiments on mice.

The invention provides a disinfection method of a ginseng explant, which comprises the following steps: soaking and disinfecting the ginseng explant by using a chemical disinfectant, soaking the soaked and disinfected explant into a first ethanol solution, taking out, igniting residual ethanol on the surface of the explant, burning, and cooling. According to the method disclosed by the invention, conventional chemical disinfection and high-concentration ethanol infiltration-flame burning are creatively combined, and the step-by-step and synergistic sterilization strategy is particularly suitable for explants with complicated structures, such as ginseng roots. Compared with a traditional disinfection method needing long-time soaking and multiple times of rinsing, the method has the advantages that the total disinfection time is greatly shortened and the explant preparation efficiency is remarkably improved by introducing an instantaneous firing step, and the explant preparation efficiency is greatly improved by reducing the total contact time of a chemical disinfectant, inactivating surface polyphenol oxidase by utilizing instantaneous high temperature and combining rapid cooling. The browning reaction is effectively inhibited, and the activity of the explant is ensured.

The invention relates to the technical field of surgical biomedical materials, and provides a method for maintaining tissue activity by hydrogel, which comprises the following steps: S1, dissolving a photo-crosslinkable natural polymer material in a solvent, and standing to obtain a first mixed solution; s2, adding a photo-initiation cross-linking agent into the first mixed solution to obtain a photo-curing adhesive precursor solution; s3, pouring the photocuring adhesive precursor solution into a pore plate mold to enable the volume of the photocuring adhesive precursor solution to be matched with the size of the tissue; and S4, completely immersing the tissue into the photocuring glue precursor solution, and carrying out photocuring by adopting in-situ light irradiation to obtain the hydrogel wrapping the tissue. The hydrogel has the advantages that the hydrogel has excellent tissue fixing performance and biocompatibility, and the original activity of a sample is kept for slice preparation under the condition that the tissue sample is not dehydrated and inactivated.

The present application relates to a kind of reversible immunomagnetic beads for quickly separating target cells and its preparation method. Specifically, the present application provides a kind of reversible immunomagnetic beads, which includes magnetic carrier, affinity ligand and the connecting arm of the connection between the two, wherein the connecting arm contains a nucleotide sequence that can be specifically recognized by enzyme cutting, preferably containing deoxyinosine (dI) base.The present application also provides a kit comprising the magnetic beads and specific cleavage enzyme (such as endonuclease V).The magnetic beads of the present application can be cleaved by enzyme to achieve mild and rapid dissociation of the magnetic beads and target cells, with high separation efficiency and good cell viability, solving the problem of cell damage caused by irreversible binding of traditional immunomagnetic beads.The present application is simple to operate, does not require complex equipment, and the separated cells can be directly used for in vitro expansion, differentiation or cell therapy, and has wide research and clinical application prospects.

The present application relates to the technical field of biological nanomaterials, in particular to a multi-level structure calcium phosphate nanomaterial, a preparation method and application thereof.The present application prepares small-scale calcium phosphate nanoparticles through a biomimetic mineralization strategy, and then crosslinks the same with L-lysine or cystamine dihydrochloride through an EDC / NHS-mediated amide coupling method to form a size-controllable multi-level structure aggregate.Compared with the small-scale calcium phosphate nanoparticles, the multi-level structure, as a doping matrix of metal ions such as Cu and Mn, significantly improves the catalytic efficiency of the metal ions, as a drug carrier, improves the loading capacity of the drug, and the multi-level structure shows a higher tumor accumulation effect.The present application has a simple preparation method, can realize the size regulation of the multi-level structure calcium phosphate nanomaterial, and can realize the tumor microenvironment-responsive disintegration of the multi-level structure.The method has good process repeatability and high batch stability, is suitable for developing high-performance nanodrugs, and has good application potential in the biomedical field.

The invention discloses a method and a device for multi-flux in-situ tracing of organoid metabolic flux based on melting DNP. The method comprises the following steps: developing an organoid vitality maintenance technology, a super target molecular probe quality control and shunting technology and combining CSI with a selective excitation technology; a set of multi-flux in-situ real-time metabolic flux tracing method suitable for the organoid is established, a multi-flux multi-metabolic small molecule metabolic flux detection technology is developed, in-situ tracing of an organoid metabolic pathway which is faster and more sensitive than that of a conventional scheme can be provided, and a new technical scheme is expected to be provided for research of an organoid model. According to the method, the metabolic flux detection tracing under the organoid in-situ culture condition can be realized, the high-flux detection tracing can be realized, and the sensitivity and the accuracy are high.

The present invention provides a method for freezing and preserving bovine semen, and relates to the technical field of semen freezing. The method for freezing and preserving bovine semen mainly comprises the following steps: feeding the breeding cattle with mixed folic acid, lycopene, anthocyanin, and anisene in the early stage, artificially collecting semen, adding caffeine, dendrobium phenol, chlorogenic acid, and pinoresinol diglucoside to a basic semen diluent, diluting the bovine semen, precooling the diluted bovine semen, and finally subjecting the precooled semen to low-temperature treatment and then freezing with liquid nitrogen. The present invention overcomes the shortcomings of the prior art, can effectively reduce the effect of freezing on the vitality of bovine semen, ensure the motility and plasma membrane integrity of bovine sperm after freezing, and provide a good foundation for cattle breeding.

The invention relates to the technical field of terahertz security check, and discloses a flat plate type terahertz human body imaging security check system and equipment, which comprises a parameter acquisition module, a parameter comparison module and a degree response module. In the secondary inspection process, the similarity of the second contraband is compared with the similarity of the first contraband, and only when the similarity of the second contraband is equal to or higher than the similarity of the first contraband, the marked suspected contraband is manually inspected. And the condition that the similarity of the second contraband is smaller than that of the first contraband is released, so that all suspected contrabands are not manually inspected once on the basis of ensuring strict inspection from reality, and the security inspection efficiency and the passing efficiency of the to-be-inspected personnel are ensured.

The present invention provides a method for improving the sexual reproduction efficiency of Suaeda salsa, and belongs to the technical field of Suaeda salsa reproduction. The method of the present invention includes collection and storage of Suaeda salsa seeds, pre-sowing vitality detection and management, and planting management; the seed collection includes collecting Suaeda salsa seeds with a moisture content of ≤10% and a purity of ≥95%; the storage conditions are temperature ≤10°C and relative humidity ≤75%; the pre-sowing vitality detection includes a germination rate of more than 85%, and the pre-sowing management includes soaking seeds in fresh water for 6-10h; the planting management includes planting in spring, applying basal fertilizer to the soil available N content of ≥120mg / kg, and irrigating fresh water once in spring and summer. The present invention manages the collection and storage of Suaeda salsa seeds, performs vitality detection before sowing, and adjusts the planting management method, so as to promote the germination of Suaeda salsa seeds and the growth of seedlings, and improve the sexual reproduction efficiency of Suaeda salsa.

The application discloses a kind of functionalized magnetic beads, which are magnetic microspheres formed by wrapping magnetic beads with bifunctional nucleic acid, and can specifically bind mRNA.A fungal mRNA extraction kit composed of functionalized magnetic beads includes grinding material, lysing solution I, lysing solution II, magnetic bead binding solution, washing solution I, washing solution II and eluent.A mRNA extraction method includes sequentially adding grinding material, lysing solution I, lysing solution II, magnetic bead binding solution, washing solution I, washing solution II and eluent after sample pretreatment to obtain mRNA product.The application breaks through the traditional mRNA extraction method, obtains high-quality mRNA, and has the advantages of simple operation, safety, high sensitivity, good reproducibility and low cost.The mRNA obtained by the application can provide high-quality templates for RT-PCR detection, improve the specificity and sensitivity of detection, and has wide application prospects in the fields of clinical diagnosis of diseases, judicial identification, food safety detection, microbial detection and molecular biology.

The present invention provides a doped iridium oxide catalyst and a preparation method and application thereof. The doped iridium oxide catalyst comprises low-crystallinity iridium oxide and a corrosion-resistant metal doped in the low-crystallinity iridium oxide; the corrosion-resistant metal is selected from any one or more of titanium, zirconium, hafnium, vanadium, niobium or tantalum. In the process of preparing the doped iridium oxide catalyst, the corrosion-resistant metal and the acid-labile metal are introduced into the precursor, and the corrosion-resistant metal is added to the IrO x During the formation process, some Ir sites are replaced, forming in-situ doping of corrosion-resistant metals (denoted as "M"), constructing a large number of Ir-O-M bridge bond interfaces, which has the effect of regulating the electronic structure of Ir and O, thereby improving the operating stability of the catalyst. In addition, the acid-labile metals are removed in the subsequent pickling process, creating abundant defect sites, which reduces the IrO x The effect of crystallinity can effectively maintain the high activity of the catalyst.

The invention provides a method for separating human umbilical cord mesenchymal stem cells. The umbilical cord mesenchymal stem cells are obtained by sequentially carrying out umbilical cord tissue treatment, umbilical cord sheet treatment and umbilical cord block culture and separation. The method disclosed by the invention is simple to operate, the separation efficiency of the umbilical cord tissue is improved, the purity of the obtained cells is higher and is obviously superior to the requirement of the ISCT lowest identification standard, the production cost can be effectively reduced, and industrial application is facilitated.

The application belongs to the technical field of biological medicine, and particularly relates to a thymus tissue dissociation reagent, a kit and application thereof in obtaining thymus epithelial cells. The thymus tissue dissociation reagent at least comprises collagenase D, dispersinase II, deoxyribonuclease I, dissociation enzyme and a dissolving reagent; and collagenase II and / or collagenase IV and / or trypsin. The cell survival rate of the thymus tissue dissociation reagent after dissociating the thymus tissue is maintained at a high level of 95.7% to 99.6%, which is significantly higher than that of the prior art; and after sorting, the number of thymus epithelial cells enriched and obtained from each gram of thymus tissue is 1.38e 5 ~1.06e 6 , and the success rate of enriching and obtaining thymus epithelial cells is as high as 95%, which is significantly higher than that of the prior art; and the proportion of epithelial stem cells in the thymus epithelial cells is 65.7%, which is significantly higher than that of the prior art.

The invention discloses a wax sealing composition for wax sealing storage of seeds and its application. The wax sealing composition is prepared by mixing 16 to 20 parts of carboxymethyl chitosan, 6 to 10 parts of polyvinyl alcohol, 6 to 14 parts of propylene glycol and 11 to 13 parts of glycerol by mass. The wax sealing composition can be used to prepare wax sealing liquid. After the seeds are dried, the dried seeds are dipped in wax with the wax sealing liquid to ensure that the surface of the seeds is all thinly waxed. After being dispersed and dried in the shade, they are placed in a ventilated, dry and light-proof place indoors for storage at room temperature or low temperature, which can extend the storage time of the seeds and maintain the vitality of the seeds. The invention provides a new energy-saving method for seed storage, which is simple, low-cost and has good effect in the actual storage of Gaozhou tea oil seeds. The invention conforms to the new idea of ​​the country to promote sustainable agricultural development, and the wax sealing composition has broad application prospects in the actual storage of seeds.

This invention discloses a method for obtaining highly active ethanol oxidase through genetic modification. A recombinant expression vector of ethanol oxidase-polyhistidine is constructed using molecular biology methods, thereby obtaining engineered bacteria expressing active recombinant oxidase. By optimizing the expression conditions of the recombinant ethanol oxidase, the activity of the ethanol oxidase produced by this engineered bacteria is improved. Measurements show that this optimized recombinant ethanol oxidase exhibits significantly improved catalytic activity for ethanol compared to wild-type ethanol oxidase, and the product demonstrates good stability, making it suitable for fermentation production.

The invention discloses a storage method and application of cymbidium seeds, and belongs to the technical field of agricultural product storage. According to the method, mixed and sterilized sandy soil and cymbidium orchid seeds are alternately treated at two temperature intervals of 2-6 DEG C and 20-30 DEG C, natural environment temperature change is simulated, seed aging is delayed, and seed vitality, storage stability and germination rate are improved. Meanwhile, mature cymbidium capsules are collected and dried in the shade till the water content of the seeds is smaller than 10%, river sand and matrix soil are mixed and sterilized to serve as a storage medium, the air permeability and the water-retaining property are guaranteed, and the infection risk is reduced; a proper humidity environment is kept by spraying a mixed sterilizing solution, so that the storage effect is further improved; and the effectiveness and the controllability of the storage process are ensured by monitoring the temperature and the humidity in real time. The method is applied to artificial breeding of cymbidium orchid and storage of orchid seeds, the germination rate and the seedling success rate are increased, the genetic stability is kept, and powerful support is provided for production, research and application of cymbidium orchid and orchid.

The hydrothermal formation all-in-one machine comprises a cuboid hangar, wherein a positive needle plate assembly, a suction nozzle rod assembly and a lifting cylinder are arranged on a top plate of the hangar; a mechanism bottom frame is arranged at the bottom of the hangar and is provided with a negative needle plate assembly; the lifting cylinder is connected with the mechanism bottom frame; a top plate of the hangar is connected with a mechanism bottom frame through guide columns, and a mechanism middle frame and a mechanism middle frame battery tray are arranged on the guide columns in a penetrating mode. Vertical side air ducts are arranged on the left side and the right side of the hangar correspondingly. Each side air duct is provided with an upper draught fan assembly, a hydrothermal plate assembly, a bottom draught fan assembly and an energy storage explosion venting exhaust fan from top to bottom. The internal temperature of the hangar for high-speed air internal circulation formation test is constant at 45 + / -2 DEG C, so that the high activity of lithium ions is ensured, the loss of electric energy is effectively reduced, the battery production efficiency is improved, and energy conservation and emission reduction are realized; and the drawable fan is convenient for workers to maintain.

The utility model relates to the technical field of enzymic preparation storage, and provides a large-scale enzymic preparation storage and addition system, which comprises an enzyme storage and addition unit, an enzyme storage and addition unit and a control unit, the enzyme storage tank comprises an enzyme storage inner container and a shell, the enzyme storage inner container is arranged in the shell, and a heat preservation assembly is arranged between the enzyme storage inner container and the shell and used for preserving heat of an enzyme preparation stored in the enzyme storage inner container; a liquid level sensor and a temperature sensor are arranged in the enzyme storage liner and are used for monitoring the storage amount and the temperature of an enzyme preparation in the enzyme storage liner in real time; the upper part of the enzyme storage liner is communicated with the feed port through an upper pipeline for supplementing an enzyme preparation, and the lower part of the enzyme storage liner is communicated with the reaction tank through a lower pipeline for adding the enzyme preparation. The device can reduce labor intensity, improve processing efficiency, accurately measure the dosage of raw materials, maintain the activity of enzyme preparations and improve the economical efficiency of processing.

The invention discloses a fully degradable pre-sowing rice pot seedling tray and a preparation method thereof, comprising a seedling tray body, nutrient soil in the seedling tray body and a degradable film above the nutrient soil; the seedling tray body comprises rice straw, cow dung, a degradable adhesive and corn starch, the nutrient soil comprises peat soil, vermiculite and compound micro-fertilizer, and the degradable film is a polyvinyl alcohol film; the seedling tray body of the invention replaces most of the soil with rice straw, which not only reduces soil collection and reduces costs, but also the plant fibers of the straw provide better physical strength for the seedling tray body, and under the bonding effect of the degradable adhesive and corn starch, the firmness of the seedling tray body is stronger and the stability is higher; and the moisture content of the seedling tray body and the nutrient soil of the invention is between 10-12%, which not only ensures the vitality of the seeds, but also facilitates the storage of the seedling tray after preparation, and has high economic benefits.

The invention discloses a synthesis method of porous polylactic acid microspheres, and belongs to the field of biomedical materials. The method comprises the following steps: preparing a first water phase taking a water-soluble polymer as a pore-foaming agent, and dispersing the first water phase in a polylactic acid organic solution to form a water-in-oil-in-water primary emulsion; introducing the primary emulsion into a curing bath containing a poor solvent, so that the organic solvent is extracted, the polylactic acid is cured, and microspheres wrapping pore-foaming agent liquid drops are formed; and finally, washing to remove the pore-foaming agent to form a porous structure. The core of the method is that the internal water-phase liquid drops are used as a sacrificial pore-forming template, and the microspheres are cured by a solvent extraction method. The method is mild in condition, green and environment-friendly, the aperture, porosity, particle size and pore connectivity of the microspheres can be independently and accurately regulated and controlled by adjusting parameters such as emulsification conditions and phase proportion, and the prepared microspheres have the characteristics of open pores and high connectivity and have excellent application prospects in the fields of drug sustained release and tissue engineering.

The invention relates to the technical field of tumor cell acquisition, in particular to a method for capturing tumor cells in whole blood based on acoustic microfluidics. Comprising the following steps: centrifuging whole blood of a tumor patient, and diluting the obtained precipitate to obtain a diluent; enabling the diluent to pass through a micro-fluidic chip, and capturing tumor cells by using an acoustic micro-fluidic technology; the voltage required by the acoustic micro-fluidic technology is 60 Vp-p, and the frequency is 3.4 KHz. According to the method, the lung adenocarcinoma A549 cells can be directly and accurately captured in whole blood, erythrocyte lysis is not needed, the captured cells can be subjected to lossless release, and the method can be used for subsequent research. The method lays an experimental foundation for further development of a method capable of directly capturing circulating tumor cells in whole blood of a lung cancer patient in the future.

The present invention discloses a closed tidal alternating anaerobic and aerobic fermentation equipment and method, which relates to the field of food processing technology. The fermentation equipment includes a tank body and a control pipe group. The tank body is divided into a fermentation chamber and a collection chamber, and the fermentation chamber is located above the collection chamber; the control pipe group includes an ascending pipeline and a descending pipeline, and the ascending pipeline and the descending pipeline can control the tide to realize aerobic and anaerobic fermentation processes. The present invention uses a closed tank body and peripheral components to effectively combine to complete the fermentation and brewing of traditional yellow wine, fruit wine, rice wine, Shanlan wine, etc. or the fermentation process of other fermented foods such as rice vinegar and soy sauce, and can achieve isolation of miscellaneous bacteria, sterilization, alternating aerobic fermentation and anaerobic fermentation, organized control of micro-oxygen source entering the fermentation mash to supply oxygen and organized control of the discharge of carbon dioxide generated inside the fermentation process of the mash, no stirring and no raking, and no damage to the yeast mycelium fermentation bed, and in the middle and late stages of fermentation, the vitality of the fermentation bacteria is maintained by supplementing and perfusing nutrient solution.