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76 results about "Slice preparation" patented technology

The slice preparation or brain slice is a laboratory technique in electrophysiology that allows the study of a synapse or neural circuit in isolation from the rest of the brain, in controlled physiological conditions. Brain tissue is initially sliced via a tissue slicer then immersed in artificial cerebrospinal fluid (aCSF) for stimulation and/or recording. The technique allows for greater experimental control, through elimination of the effects of the rest of the brain on the circuit of interest, careful control of the physiological conditions through perfusion of substrates through the incubation fluid, to precise manipulation of neurotransmitter activity through perfusion of agonists and antagonists. However, the increase in control comes with a decrease in the ease with which the results can be applied to the whole neural system.

Preparation method of improved room temperature electron ion fast transfer electrode slice for solid-state secondary lithium battery

The invention discloses a preparation method of an improved room temperature electron ion fast transfer electrode slicefor a solid-state secondary lithium battery. The method comprises the following steps: (1) evenly mixing an active material, a conductive agent and a fast ion conductor according to a certain proportion; (2) adding a certain amount of a binder into the mixture, and mixing uniformly to obtain a uniform slurry; and (3) preparing the slurry into slices, and drying to obtain the required electrode slice. The preparation method of the electrode slice preparation uses the fast ion conductor material with high temperature high lithium ion conductivity; the material can play the role of increasing the contact area between the active particles and solid electrolyte, and he form a three-dimensional electron and lithium ion transport network, so as to ensure the rapid conduction of the electrons in the electrode also improve the transmission rate of lithium ions between the active particles and electrolyte. Therefore, the preparation method is beneficial to reducing the interface impedance among the active particles in the electrode slice and between the active particles and the solid electrolyte, thereby increasing the power rate performance of the solid-state secondary lithium battery.
Owner:QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI

Whole breast tumor small-interval subserial pathological slice preparation method

The invention discloses a whole breast subserial large slice preparation method. The method comprises the following steps of collecting a tumor sample; performing continuous parallel splitting to obtain sample blocks which are split in parallel; performing soaking and fixing in a neutral formaldehyde solution with the concentration of 4% for 24 hours; removing redundant fat tissues by clipping; performing continuous parallel cutting in parallel to an original slice again to form a slice shape; performing continuous fixing for 48 hours by using the neutral formaldehyde solution with the concentration of 4%; performing sequential dehydration by adopting gradient ethanol; performing transparency treatment by using xylene; performing wax dipping in an electric heating constant temperature incubator at 62-65 DEG C to obtain a paraffin pathological sample; and slicing the paraffin pathological sample by adopting a rotary paraffin slicing machine, wherein each sample block can be subjected toconventional pathological staining including HE through continuous slicing. The thickness of a large slice prepared with the method can reach the conventional slice thickness of 4-5 microns; the slice is complete in tissue structure, smooth, clean and free of folds and pollution; no slice stripping phenomenon occurs; the cell nucleoplasm proportion is clear; and a staining result is consistent with a conventional clinical staining result.
Owner:WEST CHINA HOSPITAL SICHUAN UNIV

Ternary complex aerogel and preparation method and electrode slice preparation technology

The invention discloses a ternary complex aerogel. The aerogel is a three-dimensional porous structure formed by graphene and carbon nanotube. According to the invention, amorphous silicon is embeddedon a graphene sheet layer. The invention discloses a preparation method of the ternary complex aerogel and a preparation technology for an electrode slice. The nano amorphous silicon particles are loaded on the graphene sheet layer, and large volume effect of silicon can be buffered by a three-dimensional connected framework of the three-dimensional graphene aerogel. According to the addition ofthe carbon nanotube, one on hand, the three-dimensional network formed by the carbon nanotube and the graphene sheet layer has pinning and fettering effects for silicon particles, mutual inhibition isrealized, and agglomeration is not easily generated; on the other hand, through electrostatic action or Pi-Pi bond stacking effect, and graphene bonding carbon nanotube, the electron migration rate between the graphene sheet layer apertures can be enhanced; the addition of the carbon nanotube can greatly increase the machinery performance of the aerogel, and the aerogel can employ a compressed tablet method to prepare the self-supporting electrode slice.
Owner:涵谷新能源科技(上海)有限公司

Liquid-based thin cell slide preparation device

The invention relates to a liquid-based thin cell slide preparation device. The device comprises a rotary centrifugal mechanism and a plurality of liquid-loading slide preparation mechanisms arranged on the peripheral sides of the rotary centrifugal mechanism separately, wherein each liquid-loading slide preparation mechanism comprises a fixing base and filter pipes vertically inserted at the upper part of the fixing base, and rubber gaskets are arranged at the lower parts of the filter pipes. The invention aims to provide the liquid-based thin cell slide preparation device which is convenient to mount and dismount and good in slice preparation effect. The liquid-based thin cell slide preparation device has the advantages as follows: the liquid loading mechanisms are hinged with the rotary centrifugal mechanisms, so that cell sap in the filter pipes is perpendicularly thrown to slides, and the cell sap is uniformly spread; 2, a filter net is arranged at the lower part of each filter pipe and used for filtering the cell sap in the corresponding filtering pipe, so that cell sap entering the corresponding slide doesn't contain impurities; 3, compared with an L-shaped pipe, each straight tubular filtering pipe can contain more cell sap, and cells on each slide are more sufficient.
Owner:邱加伟

Anti-oxidation nylon 6 slice polymerization production device and method

PendingCN108570148ASolve the problem of easy mixing of oxygenSolve the problem of high dissolved oxygenNylon 6Slice preparation
The invention relates to an anti-oxidation nylon 6 slice polymerization production device and method, and belongs to the technical field of the nylon 6 slice preparation. The anti-oxidation nylon 6 slice polymerization production device comprises an oxygen-isolating distribution device, a tower-sealing polymerization device, an extraction device, a slice processing device, a pressure-control drying device, a packaging device, a desalted water deoxidizing device, and an MVR triple effect evaporating device. The oxygen-isolating distribution device is connected with the tower-sealing polymerization device. The tower-sealing polymerization device is connected with the slice processing device. The slice processing device is connected with the extraction device. The extraction device is connected with the pressure-control drying device. The pressure-control drying device is connected with the packaging device. The tower-sealing polymerization device comprises a tower-sealing compression front polymerization device and a tower-sealing decompression rear polymerization device. A material outlet of the tower-sealing decompression rear polymerization device is connected with a material inlet of the extraction device. The extraction device is connected with the MVR triple effect evaporating device. The desalted water deoxidizing device is respectively connected with the extraction deviceand the tower-sealing compression front polymerization device. The whole process adopts an anti-oxidation design, and the guarantee is provided for producing a high-quality nylon 6 product.
Owner:LIAOCHENG LUXI CHEM ENG DESIGN

Electrode slice preparation apparatus and electrode slice preparation method

The invention discloses an electrode slice preparation apparatus. The electrode slice preparation apparatus comprises an unwinding mechanism, a coating mechanism, a drying mechanism, a pressing mechanism and a winding mechanism, wherein the unwinding mechanism comprises a first unwinding device used for unwinding a current collector, and a second unwinding device used for unwinding a support base material; a through hole is formed in the current collector; the coating mechanism comprises a back-up roll and a coating apparatus that are tangent; the current collector and the support base material are fitted and then the fitted current collector and the support base material pass through the space between the back-up roll and the coating apparatus; the drying mechanism comprises a drying apparatus; the current collector and the support base material pass through the drying apparatus; the pressing mechanism comprises a first pressing roll and a second pressing roll that are tangent; the current collector and the support base material pass through the space between the first pressing roll and the second pressing roll; and the winding mechanism comprises a first winding apparatus for winding the current collector and a second winding apparatus for winding the support base material. According to the electrode slice preparation apparatus, the current collector and the support base material are mutually fitted through the back-up roll; and the overall tensile strength of the fitted current collector and the support base material is greatly increased. The invention also discloses an electrode slice preparation method.
Owner:STARZEON ELECTRONICS DONGGUAN +1

Enteromorpha clathrata rice crust and preparation process thereof

The invention discloses an enteromorpha clathrata rice crust and a preparation process thereof. The enteromorpha clathrata rice crust comprises the following components in part by weight: 80 parts of rice or coarse cereals, 5.7 parts of starch, 2 parts of vegetable oil, 10 parts of enteromorpha clathrata powder, 1.5 parts of sugar, 0.5 part of salts, 0.25 part of spices and 0.05 part of flavoring essences. The preparation process of the enteromorpha clathrata rice crust comprises the following steps of: (1) mixing and steaming of main or auxiliary materials, namely mixing the rice or the coarse cereals, the sugar, the salts, the spices and the flavoring essences in the proportion and then steaming the mixture by heating; (2) slice preparation by compressing, namely adding the starch into the mixture in the proportion and then compressing the mixture to prepare slices by using special equipment; (3) frying, namely frying the slices in the vegetable oil; (4) coating, namely coating the fried and de-oiled raw materials with the enteromorpha clathrata powder; (5) drying; and (6) weighing and packing the coated products to obtain the finished products. Added with the enteromorpha clathratas, the color the rice crust is improved and the taste and nutrition of the rice crust is improved; and moreover, the preparation process is simple and has high practicality.
Owner:FENGHUA YIZHEN SEAWEED TECH

Sticking agent for making bacterial scanning electron microscope observation slice and preparation method thereof

InactiveCN106596608AEnriching Microscopic Observation and Production TechniquesRich technical approachesMaterial analysis by measuring secondary emissionEmulsionDistilled water
A sticking agent for making a bacterial scanning electron microscope observation slice and a preparation method thereof are disclosed; every 100 ml of the sticking agent comprises 3-5mL of egg white, 5-7mL of glutinous rice powder emulsion, and balance of distilled water; the preparation method is as follows: respectively taking 3-5mL of the egg white and 5-7mL of the glutinous rice powder emulsion to put into a beaker, using sterile water to fix the volume to 100mL, evenly mixing, using a disposable needle filter with the specification of 0.22 mu m for filtering to obtain filtrate as the sticking agent; a method for making the scanning electron microscope bacterial ultramicroscopic morphology observation slice from the sticking agent comprises the steps of collection of bacteria, fixation, sticking, dehydration, replacement, critical point drying, sticking on a sample platform and ion sputtering; the effective sticking agent aiming at bacterial scanning electron microscope observation experiments and the preparation method thereof and a slice preparation method are provided, and the effective sticking agent solves slice shedding and mixing phenomena in the bacterial scanning electron microscope observation experiments.
Owner:HENAN UNIV OF SCI & TECH

RNA in-situ hybridization optimization method for cucumber seedling gene localization research

The invention relates to an RNA in-situ hybridization optimization method for cucumber seedling gene localization research. The method comprises the following steps: firstly, improving a plant culture, sampling and fixing method according to the characteristics of cucumber seedling plant materials so as to obtain a complete plant structure containing target tissues; secondly, optimizing operatingtechniques such as slice preparation, hybridization pretreatment, hybridization incubation slice sealing and chromogenic reaction microscopic examination in a targeted manner so that the reliability,accuracy and specificity of a gene localization result are improved; finally, designing a treatment scheme suitable for cucumber seedling mature tissue after the chromogenic reaction is ended in a targeted mode so that interference of cell inclusions can be eliminated, and directly observing the distribution of hybridization signals. The method is not only used for improving the sensitivity and accuracy of experimental results, but also reduces the economic cost by improving the utilization rate of drugs, reducing the consumption of experimental articles, increasing the yield of reaction products, saving the labor cost and the like, so that the method is a scientific research method considering scientificity, innovativeness and practicability.
Owner:CHINA AGRI UNIV

Dried meat slice preparation method

The invention discloses a dried meat slice preparation method. The dried meat slice preparation method comprises chopping, curing, spreading and sieving, drying and baking, and particularly comprises the following steps: taking meat paste, salt and composite phosphate according to formula amounts, adding a functional protein peptide, and performing chopping to obtain chopped meat paste; adding cooking wine, five spice powder, white sugar, glucose, glutamine transaminase, carrageenan and konjac glucomannan into the chopped meat paste according to formula amounts, performing uniform mixing and curing, taking out to obtain cured meat paste; uniformly and consistently performing spreading and sieving; and drying a sieve plate, taking out semi-finished dried meat slice, and performing cooling, baking, press-cutting and packaging to obtain dried meat slice. The dried meat slice preparation method is simple and feasible and high in mechanical operation degree; the adopted meat paste can be either ground meat or leftovers, so that the raw material utilization ratio can be improved, and industrial large-scale production is easy; and by adoption of the preparation method, the dried meat slice is favorable in flavor, greasy feeling, tenderness, water-retaining property, elasticity and cohesiveness, long in storage and shelf life and improved in quality.
Owner:ZHEJIANG QINGLIAN FOOD

Egg bread slice preparation method

The invention discloses an egg bread slice preparation method, which includes the following operations: (1) kneading 100 parts of high-gluten flour, 20-25 parts of eggs, 1-2 parts of sugar and 4-5 parts of yeast; after kneading the dough, pressing the dough multiple times and then forming the dough into strips; after forming, fermenting the dough strips at 36-38 DEG C, steaming in a pan and then taking out of the pan; (2) naturally cooling steamed bread after steaming, and then slicing into steamed bread slices with a thickness of 2-5 cm; (3) primary baking: placing the steamed bread slices ona baking plate with grids of an oven; and oil spraying: spraying vegetable oil on the surface of the steamed bread slices through an atomizing nozzle, wherein secondary vegetable oil baking: sendingthe steamed bread slices after oil spraying into the oven for baking again; and (4) after secondary baking, evenly spreading a powdery seasoning on the surface of the steamed bread slices through a screen mesh while hot. The steamed bread slices prepared by the method have delicate taste, are not greasy and are not easy to drop slag, can provide better nutrition while providing energy as leisure food, and are obviously different from the existing steamed bread slices; and the prepared steamed bread slices can be called egg bread slices.
Owner:杨凌哈牙食品有限公司

Pathological diagnosis specimen preparing and preserving device

The invention relates to a pathological diagnosis specimen preparing and preserving device, and belongs to the technical field of medical apparatus and instruments. The pathological diagnosis specimen preparing and preserving device includes a specimen preparation head, an upward-opening liquid sample bin and a specimen storage box; a slice preparation bin is arranged below a reagent addition bin; a liquid spray type top cover is arranged at the upper side of the reagent addition bin; top cover mobile rings are arranged at the left side and the right side of the liquid spray type top cover respectively; a liquid guiding-out pipe is arranged at the center of the liquid spray type top cover; a liquid dripping nozzle is arranged at the upper side of the liquid guiding-out pipe; a glass slide pressure-reduction pad is arranged at the upper side of a glass slide bearing seat; a disposable puncture head is arranged at the lower side of the glass slide bearing seat; a suction motor is arranged at the left side of a blood sample collection box; a disposable sample pipe is arranged at the upper side of the suction motor; a blood sample guiding-in pipe is arranged at the upper side of the disposable sample pipe; a switch combination station is arranged at the left side of the upward-opening liquid sample bin. The device has the advantages of complete functions and convenient use, is safe and efficient during pathological diagnosis sample and specimen acquirement, preparation and preservation, and reduces the burden of medical personnel.
Owner:SHANDONG RES INST OF TUMOUR PREVENTION TREATMENT

Automatic negative pressure control method for microfiltration membrane slice preparation and dyeing detection

The invention discloses an automatic negative pressure control method for microfiltration membrane slice preparation and dyeing detection. According to the automatic negative pressure control method for microfiltration membrane slice preparation and dyeing detection, a negative pressure forming system and a negative pressure distribution system are included; the negative pressure forming system comprises a gas negative pressure pump and a surge flask connected with the gas negative pressure pump, a waste liquor flask is connected to the front end of the surge flask, and a negative pressure detection controller is arranged on a pipeline between the surge flask and the waste liquor flask; the negative pressure distribution system comprises a negative pressure distribution device and multiple pinch valves arranged on the negative pressure distribution device, and a negative pressure pipe is connected to each pinch valve and connected with the bottom of a filter type sandwich cup; the negative pressure forming system is communicated with the negative pressure distribution device; the top ends of the filter type sandwich cups are used for adding liquid to be detected. For the automatic negative pressure control method for microfiltration membrane slice preparation and dyeing detection, specimen filling, pumping, dyeing, cleaning and other processes are completed automatically in a fully automatic mode, it is guaranteed that the negative pressure and gas flow of the pipeline are constant, and it is guaranteed that when multiple specimens work simultaneously, the basically consistent state is kept.
Owner:HUNAN TECH NEW MEDICAL SYST
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