76 results about "Slice preparation" patented technology

The invention discloses a preparation method of an improved room temperature electron ion fast transfer electrode slicefor a solid-state secondary lithium battery. The method comprises the following steps: (1) evenly mixing an active material, a conductive agent and a fast ion conductor according to a certain proportion; (2) adding a certain amount of a binder into the mixture, and mixing uniformly to obtain a uniform slurry; and (3) preparing the slurry into slices, and drying to obtain the required electrode slice. The preparation method of the electrode slice preparation uses the fast ion conductor material with high temperature high lithium ion conductivity; the material can play the role of increasing the contact area between the active particles and solid electrolyte, and he form a three-dimensional electron and lithium ion transport network, so as to ensure the rapid conduction of the electrons in the electrode also improve the transmission rate of lithium ions between the active particles and electrolyte. Therefore, the preparation method is beneficial to reducing the interface impedance among the active particles in the electrode slice and between the active particles and the solid electrolyte, thereby increasing the power rate performance of the solid-state secondary lithium battery.

A sample slice preparation device of the present invention has a microtome which slices a solid specimen by a knife, a humidifier which imparts moisture to a carrier tape, slice conveyer means for adsorbing a slice by an adsorbing force of moisture imparted to the carrier tape to feed out the slice, transfer mechanism for applying water to the surface of a slide glass to transfer the slice from the carrier tape onto the slide glass by an adsorbing force of applied water, an extension unit which heats the slide glass having the slice adsorbed thereon to evaporate the moisture, thereby extending the slice, and slide glass conveyer means for conveying the slide glass provided with the slice.

The invention discloses a preparation method of natural lemon slice and fruit powder. The preparation method comprises the following steps: (1) slicing lemon fruit and performing dipping treatment, then carrying out microwave vacuum drying till the water content of lemon fruit slices is 12wt% to 15wt%; (2) then carrying out hot-air drying on the lemon fruit slices, then drying by using a hot-air drying oven till the water content of the fruit slices is lower than or equal to 9wt%, and then packing to obtain lemon slices; and (3) finally crushing the lemon slices into 80-100 meshes through a crusher so as to obtain lemon fruit powder. The method combines vacuum microwave puffing treatment and lemon slice preparation by hot-air drying and preparation of a natural fruit powder product, the color, flavor and nutrient content of the prepared product are effectively stored, and the product quality is improved. Along with the growth of the living standard of people and attention to food safety, the preparation method can be applied to composite vegetable and fruit powder and natural health leisure food. Moreover, through vacuum microwave drying, the processing cycle is shortened, the energy is saved, and the consumption is reduced.

The invention discloses a whole breast subserial large slice preparation method. The method comprises the following steps of collecting a tumor sample; performing continuous parallel splitting to obtain sample blocks which are split in parallel; performing soaking and fixing in a neutral formaldehyde solution with the concentration of 4% for 24 hours; removing redundant fat tissues by clipping; performing continuous parallel cutting in parallel to an original slice again to form a slice shape; performing continuous fixing for 48 hours by using the neutral formaldehyde solution with the concentration of 4%; performing sequential dehydration by adopting gradient ethanol; performing transparency treatment by using xylene; performing wax dipping in an electric heating constant temperature incubator at 62-65 DEG C to obtain a paraffin pathological sample; and slicing the paraffin pathological sample by adopting a rotary paraffin slicing machine, wherein each sample block can be subjected toconventional pathological staining including HE through continuous slicing. The thickness of a large slice prepared with the method can reach the conventional slice thickness of 4-5 microns; the slice is complete in tissue structure, smooth, clean and free of folds and pollution; no slice stripping phenomenon occurs; the cell nucleoplasm proportion is clear; and a staining result is consistent with a conventional clinical staining result.

The invention discloses a device and a method for remotely obtaining a digital slice from a glass slice, and problems caused by defects of the glass slice are solved by the device. The device comprises a slide glass box assembly, a loading platform assembly, an imaging and scanning assembly, a slice taking mechanical arm assembly and a control and data processing unit, wherein the slide glass box assembly is a slide glass placing storeroom; the loading platform assembly can move three-dimensionally in horizontal, perpendicular and vertical directions and meets the focusing demand of the glass slice under an optical microscope; and the slice taking mechanical arm assembly takes out the glass slice from a slide glass box and places the glass slice on the loading platform assembly accurately. According to the invention, the technical requirement for glass slice reading technical personnel is reduced, and the digital slice preparation work can be completed by operating the device; and in addition, the device is suitable for other control modes and can complete work at the same time.

The invention discloses a ternary complex aerogel. The aerogel is a three-dimensional porous structure formed by graphene and carbon nanotube. According to the invention, amorphous silicon is embeddedon a graphene sheet layer. The invention discloses a preparation method of the ternary complex aerogel and a preparation technology for an electrode slice. The nano amorphous silicon particles are loaded on the graphene sheet layer, and large volume effect of silicon can be buffered by a three-dimensional connected framework of the three-dimensional graphene aerogel. According to the addition ofthe carbon nanotube, one on hand, the three-dimensional network formed by the carbon nanotube and the graphene sheet layer has pinning and fettering effects for silicon particles, mutual inhibition isrealized, and agglomeration is not easily generated; on the other hand, through electrostatic action or Pi-Pi bond stacking effect, and graphene bonding carbon nanotube, the electron migration rate between the graphene sheet layer apertures can be enhanced; the addition of the carbon nanotube can greatly increase the machinery performance of the aerogel, and the aerogel can employ a compressed tablet method to prepare the self-supporting electrode slice.

The invention relates to a liquid-based thin cell slide preparation device. The device comprises a rotary centrifugal mechanism and a plurality of liquid-loading slide preparation mechanisms arranged on the peripheral sides of the rotary centrifugal mechanism separately, wherein each liquid-loading slide preparation mechanism comprises a fixing base and filter pipes vertically inserted at the upper part of the fixing base, and rubber gaskets are arranged at the lower parts of the filter pipes. The invention aims to provide the liquid-based thin cell slide preparation device which is convenient to mount and dismount and good in slice preparation effect. The liquid-based thin cell slide preparation device has the advantages as follows: the liquid loading mechanisms are hinged with the rotary centrifugal mechanisms, so that cell sap in the filter pipes is perpendicularly thrown to slides, and the cell sap is uniformly spread; 2, a filter net is arranged at the lower part of each filter pipe and used for filtering the cell sap in the corresponding filtering pipe, so that cell sap entering the corresponding slide doesn't contain impurities; 3, compared with an L-shaped pipe, each straight tubular filtering pipe can contain more cell sap, and cells on each slide are more sufficient.

The invention relates to the technical fields of crystal block slicing and silicon slice preparation, in particular to a crystal block slicing method. The method comprises the following steps of: putting a silicon block to be sliced into a slicer, forming a certain included angle between the sliced surface of the silicon block to be sliced with a slicing wire mesh, and when the slicing wire mesh slices, forming point contact of steel wires in the slicing wire mesh with the side edge of the sliced surface of the silicon block. The method avoids the line contact of a single steel wire with the surface of the crystal block when the steel wires just contact the surface of the crystal block during slicing, the single steel wire forms the point contact with the surface of the crystal block, the contact area is small, the steel wires are easy to slice into the crystal block, and the slipping phenomenon when the line-contact steel wires slice the surface of the crystal block is completely avoided.

The invention relates to an anti-oxidation nylon 6 slice polymerization production device and method, and belongs to the technical field of the nylon 6 slice preparation. The anti-oxidation nylon 6 slice polymerization production device comprises an oxygen-isolating distribution device, a tower-sealing polymerization device, an extraction device, a slice processing device, a pressure-control drying device, a packaging device, a desalted water deoxidizing device, and an MVR triple effect evaporating device. The oxygen-isolating distribution device is connected with the tower-sealing polymerization device. The tower-sealing polymerization device is connected with the slice processing device. The slice processing device is connected with the extraction device. The extraction device is connected with the pressure-control drying device. The pressure-control drying device is connected with the packaging device. The tower-sealing polymerization device comprises a tower-sealing compression front polymerization device and a tower-sealing decompression rear polymerization device. A material outlet of the tower-sealing decompression rear polymerization device is connected with a material inlet of the extraction device. The extraction device is connected with the MVR triple effect evaporating device. The desalted water deoxidizing device is respectively connected with the extraction deviceand the tower-sealing compression front polymerization device. The whole process adopts an anti-oxidation design, and the guarantee is provided for producing a high-quality nylon 6 product.

A sample slice preparation device of the present invention has a microtome which slices a solid specimen by a knife, a humidifier which imparts moisture to a carrier tape, slice conveyer means for adsorbing a slice by an adsorbing force of moisture imparted to the carrier tape to feed out the slice, transfer mechanism for applying water to the surface of a slide glass to transfer the slice from the carrier tape onto the slide glass by an adsorbing force of applied water, an extension unit which heats the slide glass having the slice adsorbed thereon to evaporate the moisture, thereby extending the slice, and slide glass conveyer means for conveying the slide glass provided with the slice.

The invention discloses an electrode slice preparation apparatus. The electrode slice preparation apparatus comprises an unwinding mechanism, a coating mechanism, a drying mechanism, a pressing mechanism and a winding mechanism, wherein the unwinding mechanism comprises a first unwinding device used for unwinding a current collector, and a second unwinding device used for unwinding a support base material; a through hole is formed in the current collector; the coating mechanism comprises a back-up roll and a coating apparatus that are tangent; the current collector and the support base material are fitted and then the fitted current collector and the support base material pass through the space between the back-up roll and the coating apparatus; the drying mechanism comprises a drying apparatus; the current collector and the support base material pass through the drying apparatus; the pressing mechanism comprises a first pressing roll and a second pressing roll that are tangent; the current collector and the support base material pass through the space between the first pressing roll and the second pressing roll; and the winding mechanism comprises a first winding apparatus for winding the current collector and a second winding apparatus for winding the support base material. According to the electrode slice preparation apparatus, the current collector and the support base material are mutually fitted through the back-up roll; and the overall tensile strength of the fitted current collector and the support base material is greatly increased. The invention also discloses an electrode slice preparation method.

The invention relates to a preparation method of a ginger fermented dry wine. The preparation method is used for solving the technical problems that an existing ginger wine production process is simple, the stability of a product is poor, the functional component extraction rate is low, the alcohol content is high and the product quality standardization is difficult. The preparation method comprises the following steps: sugar liquor preparation, young ginger slice preparation, young ginger soaking sugar liquor preparation, fermentation liquor blending, inoculated fermentation, and clarification and filtering of the aged wine. The preparation method is widely applied to producing the ginger wine.

The invention discloses an enteromorpha clathrata rice crust and a preparation process thereof. The enteromorpha clathrata rice crust comprises the following components in part by weight: 80 parts of rice or coarse cereals, 5.7 parts of starch, 2 parts of vegetable oil, 10 parts of enteromorpha clathrata powder, 1.5 parts of sugar, 0.5 part of salts, 0.25 part of spices and 0.05 part of flavoring essences. The preparation process of the enteromorpha clathrata rice crust comprises the following steps of: (1) mixing and steaming of main or auxiliary materials, namely mixing the rice or the coarse cereals, the sugar, the salts, the spices and the flavoring essences in the proportion and then steaming the mixture by heating; (2) slice preparation by compressing, namely adding the starch into the mixture in the proportion and then compressing the mixture to prepare slices by using special equipment; (3) frying, namely frying the slices in the vegetable oil; (4) coating, namely coating the fried and de-oiled raw materials with the enteromorpha clathrata powder; (5) drying; and (6) weighing and packing the coated products to obtain the finished products. Added with the enteromorpha clathratas, the color the rice crust is improved and the taste and nutrition of the rice crust is improved; and moreover, the preparation process is simple and has high practicality.

A sticking agent for making a bacterial scanning electron microscope observation slice and a preparation method thereof are disclosed; every 100 ml of the sticking agent comprises 3-5mL of egg white, 5-7mL of glutinous rice powder emulsion, and balance of distilled water; the preparation method is as follows: respectively taking 3-5mL of the egg white and 5-7mL of the glutinous rice powder emulsion to put into a beaker, using sterile water to fix the volume to 100mL, evenly mixing, using a disposable needle filter with the specification of 0.22 mu m for filtering to obtain filtrate as the sticking agent; a method for making the scanning electron microscope bacterial ultramicroscopic morphology observation slice from the sticking agent comprises the steps of collection of bacteria, fixation, sticking, dehydration, replacement, critical point drying, sticking on a sample platform and ion sputtering; the effective sticking agent aiming at bacterial scanning electron microscope observation experiments and the preparation method thereof and a slice preparation method are provided, and the effective sticking agent solves slice shedding and mixing phenomena in the bacterial scanning electron microscope observation experiments.

The invention relates to an RNA in-situ hybridization optimization method for cucumber seedling gene localization research. The method comprises the following steps: firstly, improving a plant culture, sampling and fixing method according to the characteristics of cucumber seedling plant materials so as to obtain a complete plant structure containing target tissues; secondly, optimizing operatingtechniques such as slice preparation, hybridization pretreatment, hybridization incubation slice sealing and chromogenic reaction microscopic examination in a targeted manner so that the reliability,accuracy and specificity of a gene localization result are improved; finally, designing a treatment scheme suitable for cucumber seedling mature tissue after the chromogenic reaction is ended in a targeted mode so that interference of cell inclusions can be eliminated, and directly observing the distribution of hybridization signals. The method is not only used for improving the sensitivity and accuracy of experimental results, but also reduces the economic cost by improving the utilization rate of drugs, reducing the consumption of experimental articles, increasing the yield of reaction products, saving the labor cost and the like, so that the method is a scientific research method considering scientificity, innovativeness and practicability.

The invention discloses a health vinegar-egg slice and a preparation method thereof. The health vinegar-egg slice comprises, by weight, 48 to 55% of vinegar-egg powder, 26 to 35% of a filler, 2 to 4% of a lubricating agent and 6 to 15% of a flavoring agent. The preparation method comprises (1) pretreatment: washing fresh eggs and soaking the fresh eggs in chlorine water solution for 5 to 30min, (2) vinegar-egg liquid preparation: breaking fresh egg shells, taking egg white and yolk, uniformly stirring the egg white and yolk, adding vinegar into the mixture, carrying out uniform mixing, carrying out soaking at a temperature of 15-50 DEG C for 12-72h, and filtering the mixture to obtain a vinegar-egg liquid, (3) vinegar-egg powder preparation, (4) vinegar-egg slice preparation: adding the filler, lubricating agent and flavoring agent into the vinegar-egg powder and processing the health vinegar-egg slice finished product. The preparation method is simple and easy and has short time consumption.

An embodiment of the invention discloses an agilawood fragrance style dry-method slice preparation method which can effectively reduce the content of wood gas and harmful substances in a dry-method slice so as to enable the dry-method slice to have a strong agilawood fragrance style. The method comprises the following steps: soaking tobacco stems in water to obtain soaked tobacco stems; carrying out bio-enzyme processing on the soaked tobacco stems, and drying the processed tobacco stems to obtain the tobacco stems subjected to the bio-enzyme processing; laying the tobacco stems subjected to the bio-enzyme processing onto a forming net after mechanical fiber separating to form a fiber thin layer; spraying chitosan adhesive on the surface of the fiber thin layer and carrying out drying to obtain a dry-method slice substrate; spraying aloewood powders on the surface of the dry-method slice substrate; spraying cigarette powders on the surface of the dry-method slice substrate; spraying chitosan on the surface of the dry-method slice substrate; and then, carrying out drying to finally obtain the dry-method slice which has the strong agilawood fragrance style, and of which the content of the harmful substances is reduced.

An embodiment of the invention discloses an agilawood fragrance style dry-method slice preparation method which can effectively reduce the content of wood gas and harmful substances in a dry-method slice so as to enable the dry-method slice to have a strong agilawood fragrance style. The method comprises the following steps: soaking tobacco stems in water to obtain soaked tobacco stems; carrying out bio-enzyme processing on the soaked tobacco stems, and drying the processed tobacco stems to obtain the tobacco stems subjected to the bio-enzyme processing; laying the tobacco stems subjected to the bio-enzyme processing onto a forming net after mechanical fiber separating to form a fiber thin layer; spraying chitosan adhesive on the surface of the fiber thin layer and carrying out drying to obtain a dry-method slice substrate; spraying aloewood powders on the surface of the dry-method slice substrate; spraying cigarette powders on the surface of the dry-method slice substrate; spraying chitosan on the surface of the dry-method slice substrate; and then, carrying out drying to finally obtain the dry-method slice which has the strong agilawood fragrance style, and of which the content of the harmful substances is reduced.

The invention relates to a guava fruit slice preparation method and solves the technical problem that guava fruits and guava leaves are not put into industrial production to produce instant food currently. The guava fruit slice preparation method includes the steps of cleaning the guava fruits and the guava leaves, cutting the guava fruits and the guava leaves into pieces, mixing with stirring to form puree, subjecting the puree to primary colloid milling, mixing with a helicase solution, and subjecting the mixture to secondary colloid milling, quick freezing, vacuum lyophilizing and slicing. The guava fruit slice preparation method is widely applied to guava food preparation.

The invention discloses a dried meat slice preparation method. The dried meat slice preparation method comprises chopping, curing, spreading and sieving, drying and baking, and particularly comprises the following steps: taking meat paste, salt and composite phosphate according to formula amounts, adding a functional protein peptide, and performing chopping to obtain chopped meat paste; adding cooking wine, five spice powder, white sugar, glucose, glutamine transaminase, carrageenan and konjac glucomannan into the chopped meat paste according to formula amounts, performing uniform mixing and curing, taking out to obtain cured meat paste; uniformly and consistently performing spreading and sieving; and drying a sieve plate, taking out semi-finished dried meat slice, and performing cooling, baking, press-cutting and packaging to obtain dried meat slice. The dried meat slice preparation method is simple and feasible and high in mechanical operation degree; the adopted meat paste can be either ground meat or leftovers, so that the raw material utilization ratio can be improved, and industrial large-scale production is easy; and by adoption of the preparation method, the dried meat slice is favorable in flavor, greasy feeling, tenderness, water-retaining property, elasticity and cohesiveness, long in storage and shelf life and improved in quality.

The invention provides a Large-breadth rock core slice preparation and a manufacturing method thereof. The manufacturing method comprises the following steps: sectioning a columnar rock core sample along a length direction to obtain the rock core sample with a first cutting surface; fixing the rock core sample on a hard base, mounting the hard base on a cutting machine, performing cutting processing on the rock core sample to obtain a rock core slice with a second cutting surface, and enabling the second cutting surface to be parallel to the first cutting surface; separating the rock core slice from the hard base; and adhering the rock core slice to a transparent matrix to obtain a large-breadth rock core slice preparation. By performing sectioning and cutting processing by using differentways, the size of the obtained rock core slice preparation can be much greater than the size of the existing rock core slice preparation, and the direct observation, the display and the long-term storage are convenient. And meanwhile, the cutting surface damage of the rock core sample in the cutting process is effectively reduced, and the waste of the important rock core and the key position andlike geological document is avoided.

The invention discloses an egg bread slice preparation method, which includes the following operations: (1) kneading 100 parts of high-gluten flour, 20-25 parts of eggs, 1-2 parts of sugar and 4-5 parts of yeast; after kneading the dough, pressing the dough multiple times and then forming the dough into strips; after forming, fermenting the dough strips at 36-38 DEG C, steaming in a pan and then taking out of the pan; (2) naturally cooling steamed bread after steaming, and then slicing into steamed bread slices with a thickness of 2-5 cm; (3) primary baking: placing the steamed bread slices ona baking plate with grids of an oven; and oil spraying: spraying vegetable oil on the surface of the steamed bread slices through an atomizing nozzle, wherein secondary vegetable oil baking: sendingthe steamed bread slices after oil spraying into the oven for baking again; and (4) after secondary baking, evenly spreading a powdery seasoning on the surface of the steamed bread slices through a screen mesh while hot. The steamed bread slices prepared by the method have delicate taste, are not greasy and are not easy to drop slag, can provide better nutrition while providing energy as leisure food, and are obviously different from the existing steamed bread slices; and the prepared steamed bread slices can be called egg bread slices.

The invention relates to a pathological diagnosis specimen preparing and preserving device, and belongs to the technical field of medical apparatus and instruments. The pathological diagnosis specimen preparing and preserving device includes a specimen preparation head, an upward-opening liquid sample bin and a specimen storage box; a slice preparation bin is arranged below a reagent addition bin; a liquid spray type top cover is arranged at the upper side of the reagent addition bin; top cover mobile rings are arranged at the left side and the right side of the liquid spray type top cover respectively; a liquid guiding-out pipe is arranged at the center of the liquid spray type top cover; a liquid dripping nozzle is arranged at the upper side of the liquid guiding-out pipe; a glass slide pressure-reduction pad is arranged at the upper side of a glass slide bearing seat; a disposable puncture head is arranged at the lower side of the glass slide bearing seat; a suction motor is arranged at the left side of a blood sample collection box; a disposable sample pipe is arranged at the upper side of the suction motor; a blood sample guiding-in pipe is arranged at the upper side of the disposable sample pipe; a switch combination station is arranged at the left side of the upward-opening liquid sample bin. The device has the advantages of complete functions and convenient use, is safe and efficient during pathological diagnosis sample and specimen acquirement, preparation and preservation, and reduces the burden of medical personnel.

A cassette stack comprises individual detachable plastic cassettes (2) which are provided to prepare histological preparations for a subsequent cutting preparation. The stack is inserted into a magazine (3) of a pressure system. The cassettes are joined together using an adhesive strip (4) and / or a welding seam. Preferred Features: The cassettes have a rectangular base surface (6) with peripherally molded side surfaces (7) and are arranged against each other in the longitudinal direction. The cassettes are arranged in he stack at an angle of at least 20 degrees to each other.

The invention discloses an automatic negative pressure control method for microfiltration membrane slice preparation and dyeing detection. According to the automatic negative pressure control method for microfiltration membrane slice preparation and dyeing detection, a negative pressure forming system and a negative pressure distribution system are included; the negative pressure forming system comprises a gas negative pressure pump and a surge flask connected with the gas negative pressure pump, a waste liquor flask is connected to the front end of the surge flask, and a negative pressure detection controller is arranged on a pipeline between the surge flask and the waste liquor flask; the negative pressure distribution system comprises a negative pressure distribution device and multiple pinch valves arranged on the negative pressure distribution device, and a negative pressure pipe is connected to each pinch valve and connected with the bottom of a filter type sandwich cup; the negative pressure forming system is communicated with the negative pressure distribution device; the top ends of the filter type sandwich cups are used for adding liquid to be detected. For the automatic negative pressure control method for microfiltration membrane slice preparation and dyeing detection, specimen filling, pumping, dyeing, cleaning and other processes are completed automatically in a fully automatic mode, it is guaranteed that the negative pressure and gas flow of the pipeline are constant, and it is guaranteed that when multiple specimens work simultaneously, the basically consistent state is kept.

The invention provides an FAMA (Fluorescent Antibody to Membrane Antigen) antigen slice preparation and preservation method for detecting varicella zoster virus (VZV) antibodies. The method comprisesthe following steps: infecting 2BS cells with varicella zoster virus strains, incubating in a 5% of CO2 incubator at a temperature of 35 DEG C to reach a virus replication peak, performing trypsin digestion and cell counting, selecting an appropriate cell quantity to prepare virus cell suspension, dropping the suspension onto a 12-well glass slide, incubating in a 5% of CO2 incubator at a temperature of 37 DEG C, performing cold acetone fixation, cleaning, drying, dropping a protective fluid on the cell surface to seal, and vacuumizing. The antigen slices can be preserved in a refrigerator of2-8 DEG C for one year or longer.

The invention discloses a preparation method of a temporary slice of a mature corn root system, belonging to the technical field of preparation of slices of plant root systems. Aiming at the problemsin the existing temporary slice preparation process that an image is unclear and leading to observational error, the invention provides the preparation method of the temporary slice of the mature cornroot system, which is characterized in that a dyed slice is subjected to vacuumizing treatment so as to remove 99% of bubbles in the slice, so that an observation image is more clear, error is reduced, and convenience is provided for research on anatomical structures of the mature corn root system.

The invention provides a cutting device for preparing soil slices, and a soil slice preparation device, and relates to the technical field of soil detection equipment. The cutting device comprises anoperation platform, a cutting assembly and a bearing assembly; the cutting assembly comprises a driving mechanism and a plurality of cutting knives which are arranged at intervals; one side of each cutting knife is connected with the output end of the driving mechanism so as to drive the cutting knife to move in a vertical direction; the bottom end of each cutting knife is a cutting end; the driving mechanism is located on one side of the operation platform; the operation platform is provided with cutting grooves corresponding to the cutting knives; the bearing assembly comprises a bearing plate; and a plurality of limiting grooves corresponding to the cutting grooves are formed in the bearing plate. The soil slice preparation device comprises the cutting device, and adapts to large-batchsample preparation; and the slice preparation efficiency is remarkably improved.

The invention discloses a method for preparing tissue slices. The method comprises the following steps: 1) fixing a tissue block: fixing the tissue block in a fixing agent; 2) dehydrating: sequentially arranging the tissue block in dioxane aqueous solutions from a lower concentration to a higher concentration for dehydrating; 3) paraffin infiltration coating: completely immersing the dehydrated tissue block into molten paraffin and coating the tissue block by the paraffin; 4) slicing and drying: drying the tissue block, adding the slices into warm water, enabling the slices to be attached into a glass slide coated with a tissue slice adhesive after the slices are fully expanded, and drying; 5) rehydrating: sequentially arranging the tissue block in dioxane aqueous solutions from a higher concentration to a lower concentration for hydrating; 6) dyeing: dyeing the slices by using a dyeing agent; 7) transparentizing, namely immersing the dyed slices into xylene to deparaffinate the slices; and 8) storing. According to the method disclosed by the invention, the slice preparation step is reduced, the slice preparation time is shortened, and tissue block contraction is avoided due to dioxane, so that the quality of the slices is high.

The invention provides a technology for detecting TK6 cell chromosome damage genetic toxicity caused by environmental water and an application of the technology and belongs to the fields of environmental science and health risk assessment. The technology for detecting the TK6 cell chromosome damage genetic toxicity caused by the environmental water comprises a pre-treatment process on an environmental water sample, a cell culture and contamination method, a micronucleus slice preparation process and a result analysis method. The provided micronucleus experiment method can be directly applied to monitoring of the genetic toxicity of the environmental water, and important genetic toxicity effect information is provided for water pollution health risk management.