This utility model discloses a device for separating guava pulp and seeds, including a separation tank. A separation component is connected to one side of the separation tank. The separation component includes a sealed bearing, and a positioning rod is connected to the inner ring of the sealed bearing. One end of the positioning rod is connected to a power motor. Two slide rails are formed on the inner side wall of the separation tank. This device uses a positioning block that matches the slide rails, and an annular groove that matches the positioning block and communicates with the slide rails. This allows for easy disassembly and installation of the separation chamber and facilitates internal cleaning. Combined with the pushing force of an electric push rod, the separation rod can be inserted into the separation chamber and the inside of the guava. A support plate covers the separation chamber, enabling it to rotate at high speed. This high-speed rotation of the separation chamber allows guava seeds to fly out from the holes in the separation chamber and be stored in the separation tank. This increases the efficiency and quality of pulp and seed separation while reducing labor.