The invention discloses a synthetic adhering culture medium for cell culture. Each liter of synthetic adhering culture medium is prepared from components as follows: 0.08 g of biotin, 20 mu g of corticosterone, 2 g of L-carnitine, 1.2 g of linoleic acid, 1.2 g of ethanolamine, 0.8 g of linolenic acid, 13-17 g of D-glucose, 5 mu g of progesterone, 1.2 g of sodium pyruvate, 0.08 g of retinyl acetate, 4 g of catalase, 0.9-1.2 g of folic acid, 0.8-1.3 g of reduced glutathione, 60-70 mu g of lipoic acid, 2-3 g of transferrin, 6-8 g of human insulin, 0.1 mg of sodium selenite, 500-600 mL of a banana peel extract, 1.2-2 g of naphthylacetic acid and 0.8-1.5 g of vitamin C. According to the synthetic adhering culture medium for cell culture and a preparation method of the culture medium, no animal serum is contained, the stem cell differentiation culture time is greatly shortened and the experiment cost is saved.