Medium for the redifferentiation of dedifferentiated chondrocytes into chondrocytes

Abstract

It is intended to provide a medium for the redifferentiation of dedifferentiated chondrocytes into chondrocytes and a method of redifferentiating dedifferentiated chondrocytes into chondrocytes whereby dedifferentiated chondrocytes, which have been dedifferentiated by, for example, culturing in vitro and thus show weakened chondral characteristics, can be efficiently and easily redifferentiated into the original chondrocytes. That is to say, a medium for the redifferentiation of dedifferentiated chondrocytes into chondrocytes which is to be used for redifferentiating dedifferentiated chondrocytes having been dedifferentiated and thus showing weakened chondral characteristics into the original chondrocytes and contains insulin together with at least one member selected from among BMP-2 and its analogs; and a method of redifferentiating dedifferentiated chondrocytes into the original chondrocytes by culturing the dedifferentiated chondrocytes with the use of the medium for the redifferentiation of dedifferentiated chondrocytes into chondrocytes. In a preferred embodiment, the above-described medium for the redifferentiation of dedifferentiated chondrocytes into chondrocytes further contains T3.

Description

technical field [0001] The present invention relates to a medium for redifferentiation of dedifferentiated chondrocytes into chondrocytes for redifferentiation of dedifferentiated chondrocytes that are moderately dedifferentiated in in vitro culture and have weakened cartilage characteristics into original chondrocytes. Background technique [0002] Cartilage constitutes the ear, nose, trachea, joints, intervertebral discs, etc., and plays an important role in maintaining the shape of the body together with bones and performing daily activities. If such cartilage is damaged by trauma such as articular cartilage damage, age-related diseases such as osteoarthritis, inflammatory diseases such as joint rheumatism, major diseases after tumor surgery, or congenital abnormalities, life activities such as walking become difficult or Inability to maintain body shape, etc., and bring about significant obstacles to daily life. The number of patients with these diseases and the like re...

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Problems solved by technology

[0004] In the above-mentioned cartilage regenerative medicine, it is necessary to establish an effective culture technology for chondrocytes. Although there are many reports on the culture and proliferation methods of chondrocytes (see Patent Documents 1 and 2), chondrocytes are depleted with a high probability during culture. Differentiate and change into fibroblast-like, but as a corresponding strategy and solution to the situation in which the chondrocytes dedifferentiate and change into fibroblast-like dedifferentiated chondrocytes, it is proposed to use hydrostatic pressure to make dedifferentiated chondrocytes Methods of redifferentiation of chondrocytes, methods of performing three-dimensional culture to redifferentiate them into dedifferentiated chondrocytes, etc. However, in the case of these methods, the present situation is that the above-mentioned dedifferentiated chondrocytes cannot be redifferentiated efficiently and easily. original chondrocytes

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