53 results about "Osteoarthritis" patented technology

This invention discloses a protein-polysaccharide ternary nanoparticle loaded with cordycepin, its preparation method, and its applications. The invention employs a layer-by-layer self-assembly method to modify the surface of zein nanoparticles. Since zein has a positive surface charge, the first layer modification uses the anionic polysaccharide sodium alginate for electrostatic bonding, and the second layer modification uses the cationic polysaccharide chitosan, constructing positively charged ternary composite nanoparticles. These nanoparticles have small particle size, high encapsulation efficiency, high drug loading capacity, and a sustained-release effect, significantly reducing cytotoxicity and significantly improving the therapeutic effect on osteoarthritis. This invention develops a novel cordycepin formulation, overcoming the limitations of cordycepin's clinical use.

The invention relates to the technical field of computer-aided teaching and medical image recognition, and particularly discloses an intelligent knee osteoarthritis image analysis teaching system based on computer vision. The system comprises an image data management module, a student interaction and behavior acquisition module, a multi-dimensional skill quantitative analysis module, a personalized knowledge graph construction and gap analysis module, a self-adaptive teaching path planning module and a teaching feedback and report generation module. By collecting and quantitatively analyzing operation behaviors and sight tracks of students, a personal knowledge graph is constructed to accurately position skill defects, and a personalized teaching path is planned according to the skill defects, so that refined evaluation and adaptive teaching guidance of image analysis practical skills are realized.

The application discloses application of mesenchymal stem cell spheroids in preparation of a preparation for treating osteoarthritis, and relates to the technical field of cell therapy and osteoarthritis treatment, and specifically comprises mesenchymal stem cell spheroids with an average diameter of 120-180 mu m and a solution containing 0.5%-10% human serum albumin. The cells are prepared into 120-180 mu m cell spheroids, and the solution containing specific concentration human serum albumin is used as a carrier, so that the cell survival rate is stabilized at more than 90%, and high-activity delivery is realized; the optimized size ensures smooth injection and long-term retention in the joint cavity, and overcomes the shortcoming that single cells are easily removed; after joint cavity injection, the preparation can synergistically promote bone reconstruction and cartilage repair, and produces clear curative effects such as bone mass increase of more than 30%, cartilage degeneration score reduction of more than 50%, and the like, which are quantifiable and verifiable, so that a stable, long-acting and curative drug preparation and application scheme are provided for osteoarthritis treatment.

The invention discloses a knee joint force line adjusting system and method based on intelligent feedback control, and relates to the technical field of medical rehabilitation instruments. The system comprises an overall control and man-machine interaction end, a correction device sensor end and a correction device hardware platform end. The hardware platform end comprises a joint force applying module and a thigh and shank multi-degree-of-freedom motion module; the sensor end is used for detecting joint pressure and lower limb force line state in real time. On the basis of traditional three-point type orthopedics, the applied force is adjusted through the control module according to real-time feedback, and the shank is matched for synchronous stretching and rotating movement, so that the knee joint force line is dynamically adjusted. In addition, the system combines multi-modal data, constructs a closed-loop decision model by using deep learning and large model reasoning technologies, and outputs correction effect evaluation and next training parameter recommendation. The digital and intelligent knee joint correction system realizes digitization and intelligentization of knee joint correction, can effectively enlarge joint gaps, improves stress distribution, and provides a personalized and safe rehabilitation treatment scheme for middle-aged and elderly osteoarthritis patients.

Provided are a chondrogenic stem cell for promoting joint repair and use thereof. Specifically, disclosed is a cell preparation used for alleviating or treating osteoarthritis, comprising a Procr-positive pluripotent stem cell. The Procr-positive chondrogenic stem cell can differentiate and generate downstream chondrocytes in vivo and promote cartilage repair in osteoarthritis damage, which will be a more effective cell treatment method. In addition, Procr proteins can be used as a new target of a drug for treating osteoarthritis.

The utility model discloses an osteoarthritis middle-symptom joint fixator which comprises an upper support, a lower support and a connecting mechanism connected between the upper support and the lower support. The upper support comprises an upper support arm corresponding to the side face of the leg, an upper surrounding part connected to the upper support arm and extending towards the front side of the thigh in an arc mode, and a first upper connecting belt matched with the upper surrounding part to bind the upper support to the leg, and the first upper connecting belt is detachably assembled with the upper surrounding part and / or the upper support arm through a quick snap fastener. The lower support comprises lower support arms corresponding to the side faces of the legs, lower enclosures connected to the lower support arms and extending towards the rear sides of the shanks in an arc mode, and first lower connecting belts matched with the lower enclosures to bind the lower support to the legs, and the first lower connecting belts are detachably assembled with the lower enclosures and / or the lower support arms through quick snap fasteners. The joint fixator for osteoarthritis can be stably worn on the legs of a user and prevented from loosening, and the joint fixator for osteoarthritis can be quickly worn through quick disassembly and assembly of the quick snap fasteners.

The invention belongs to the technical field of pharmaceutical preparations, and particularly relates to a composition for treating osteoarthritis and a preparation method thereof. The composition for treating osteoarthritis is prepared from the following raw materials in parts by weight: 1 to 3 parts of non-denatured type II collagen, 0.3 to 0.5 part of cowherb seed cyclic peptide A and 0.4 to 0.7 part of balanosine. According to the composition, through the synergistic effect of multiple active ingredients, the osteoarthritis symptom is jointly relieved, and joint tissue repair is promoted.

The application discloses a kind of food relieving bone joint damage evaluation method, it is related to food efficacy evaluation technical field, for solving the problem of single dimension of existing evaluation method, unstable model leads to poor reliability of conclusion.The application builds composite modeling and multi-modal analysis mechanism, first, a stable animal model is established by using chemical injury combined with forced exercise training, a two-stage increasing dosing regimen is set, and behavior dynamic trend data and histology, imaging multi-modal results are collected synchronously.Then, based on the weight assigned to osteoarthritis stage, a weighted composite score is generated, and a perturbation sensitivity index is calculated, a multi-index collaborative influence map is constructed, a key evaluation node is located, a graded evaluation conclusion is generated based on the food action characteristics and efficacy determination threshold, and false positives caused by single index fluctuations are avoided.Finally, a full-cycle closed-loop evaluation process is formed, which improves the scientificity, reproducibility and self-adaptive ability of efficacy determination in complex biological systems.

The invention provides a knee joint bionic lower limb exoskeleton for osteoarthritis rehabilitation, and relates to the technical field of exoskeleton robots, the exoskeleton robot comprises a waist mechanism, a hip joint mechanism, a knee joint bionic mechanism and a leg mechanism; the waist mechanism is adaptive to different body types through an adjustable waistline adjuster and an adjustable waist height adjuster; the hip joint mechanism transmits power to the front of the thigh protector through a motor driving connecting rod; the knee joint bionic mechanism adopts a sliding square block and damping coupling design to simulate a migration track of an instantaneous rotation center of the knee joint of a human body; the leg mechanism is of an orthotropic chain structure and is combined with a flexible protection pad to achieve rigid force transmission in the vertical direction and flexible adaptation in the horizontal direction. The problem that a traditional exoskeleton is insufficient in bionic property and has binding defects is effectively solved, and the exoskeleton is suitable for rehabilitation training of knee osteoarthritis patients.

The application provides a temporomandibular joint distraction soft tissue traction device, which comprises an X-axis connecting mechanism and a Y-axis adjusting mechanism, the X-axis connecting mechanism further comprises an arch strip, an abutment structure and an arc-shaped abutment distraction mechanism for hooking the patient's jaw bone, the arch strip is vertically connected with the Y-axis adjusting mechanism, the abutment structure comprises two independent ladder blocks arranged above the arch strip, the upper surfaces of the two independent ladder blocks are sequentially provided with a U-shaped support for clamping sliding, a sliding rod, a clamping buckle and a limiting hole, wherein the U-shaped supports are respectively installed at the upper ends of the two independent ladder blocks, and the arch strip and the abutment structure are adopted to promote the middle-aged and old patients suffering from temporomandibular joint osteoarthritis to seek medical treatment, and an operable space is established during the open surgery of the temporomandibular joint (similar to the state of the bag opening being adapted), and the load bearing rod, the clamping buckle, the sliding rod and the specific sliding structure state can be used for the distraction of the temporomandibular joint region.

The application relates to a hyaluronic acid-based quercetin conjugate micelle and a preparation method and application thereof, and comprises the following steps: adding a coupling agent into a hyaluronic acid solution to carry out an activation reaction, then adding a phenylboronic acid solution to carry out a grafting reaction, and carrying out purification treatment to obtain HA-PBA; mixing HA-PBA and quercetin in an organic solvent, carrying out grafting reaction and purification treatment on the obtained mixed solution to obtain the hyaluronic acid-based quercetin conjugate micelle (HPQ). The hyaluronic acid-based quercetin conjugate micelle and KGN can also be used to prepare the hyaluronic acid-based quercetin conjugate micelle loaded with KGN (HPQ@K) through a loading reaction; the prepared HPQ and HPQ@K have a spherical structure, can make the interosseous joints reach a super-lubrication state, and can improve the solubility of quercetin and KGN, and improve the treatment and repair effect of osteoarthritis.

Composition for improving inflammation in osteoarthritis, characterized in that the composition comprises the following components, measured in parts by weight: 1 to 5 parts by weight of N-acetylglucosamine and 0.1 to 2 parts by weight of calcium element in a calcium source.

The application discloses a knee osteoarthritis image prediction method and system based on a diffusion model. The method comprises the following steps: acquiring multi-modal data of a patient in a baseline period, wherein the multi-modal data comprises X-ray images, MRI sequences and clinical features; adopting a latent diffusion model as a basic prediction network architecture; adding a multi-modal fusion module and a cross-attention mask regulation in a U-Net structure of the latent diffusion model; selecting an AdamW optimizer; performing end-to-end training on the model; inputting the multi-modal data into the trained latent diffusion model; completing feature fusion through adaptive gating; and performing inverse diffusion denoising under the constraint of an inflation joint gap key point mask, and finally decoding and outputting a predicted knee X-ray image. The application can improve the accuracy of joint image prediction.

The application belongs to the technical field of biotechnology, and discloses application of a new sialyloligosaccharide and astaxanthin composition in preparation of a transdermal drug for treating osteoarthritis and a composite gel preparation. The composition provided by the application comprises new sialyloligosaccharide, astaxanthin, carbomer and alginate with a mass ratio of (1-10):(1-10):(0.01-0.5):(0.1-1). The composition can realize efficient enrichment of the new sialyloligosaccharide and astaxanthin in the joint cavity through a diffusion pathway of skin penetration, deep tissue diffusion, joint capsule penetration and joint cavity enrichment of the substance, and the new sialyloligosaccharide and astaxanthin have excellent synergistic effects, can efficiently relieve acute inflammatory symptoms of gouty osteoarthritis, block the disease progression and repair the joint tissue pathological changes, and have excellent application prospects in preparation of the transdermal drug for treating osteoarthritis.

The invention discloses a medical composite material for synergistically regulating cholesterol and oxidative stress. The material comprises a Fe2-N6 / NC carrier, cholesterol oxidase loaded on the Fe2-N6 / NC carrier and a hyaluronic acid targeting layer modified on the surface, the carrier is a nitrogen-doped carbon material loaded with iron double-atom active sites, the distance between adjacent iron atoms is 0.311 + / -0.009 nm, and the iron content is 0.393 wt%; the average particle size of the composite material is 242.5 nm, and the Zeta potential of the composite material is-23.71 mV; the preparation method comprises the following steps: synthesizing Fe2-N6 / NC through mechanochemical-assisted in-situ MOF packaging, ultrasonically loading cholesterol oxidase and modifying with hyaluronic acid. The material has cholesterol degradation activity (the degradation rate is 33.94%) and active oxygen scavenging activity (the scavenging rate is 78.8%), and can be used for preparing an implant material for joint repair or a medical material for articular cavity injection so as to realize coordinated regulation of osteoarthritis cholesterol metabolism disorder and oxidative stress.

The invention provides a nanoparticle composition based on perinatal tissue as well as a preparation method and application thereof, and relates to the technical field of biological medicines. The nanoparticle composition disclosed by the invention is obtained by decellularizing and purifying perinatal tissues. The nano-particle composition obtained by the invention contains rich cell factors and growth factors and more diverse miRNA (micro Ribonucleic Acid) components, and is applied to related joints. Clinical tests carried out in the sports and joint medicine department of the tenth people hospital in Shanghai and a rat model are passed, after the nano-particle composition is injected into the rat model, the pain and function of osteoarthritis can be effectively improved, and no adverse event is generated. The invention provides a novel safe and effective treatment way for promoting tissue repair and regeneration.

This invention proposes a method for constructing an animal model for the prevention of temporomandibular joint osteoarthritis (TMJ) by knocking out the FAM20C gene, belonging to the field of pharmaceutical technology. It solves the problem of providing effective treatment and prevention methods for TTMJ osteoarthritis. It includes the application of the FAM20C gene in the preparation of drugs for the prevention or treatment of TTMJ osteoarthritis. It is mainly used for the prevention or treatment of TTMJ osteoarthritis.

A chitosan thermosensitive glue composition for treating osteoarthritis and a preparation method thereof. The composition comprises 0.5-5% chitosan thermosensitive glue and 10 6 -10 7 / mL stem cells. After mixing mesenchymal stem cells with chitosan thermosensitive glue, the mixture is injected into the joint cavity, which can not only play the effect of chitosan gel in lubricating the joint, but also inhibit inflammatory factors secreted by mesenchymal stem cells, and at the same time, mesenchymal stem cells can promote the regeneration of chondrocytes, effectively treating osteoarthritis.

The invention relates to the technical field of biomedicine, in particular to a preparation method and application of wrinkled Nb2CTx-SeNPs based on a physical-chemical cooperative signal. The core of the preparation method disclosed by the invention is to realize the synergism of physical and chemical signals, and the nanoscale fold topological structure constructed by a special process can provide unique physical stimulation for the macrophage and guide the functional phenotype of the macrophage. Meanwhile, the anti-inflammatory potential of the substrate material Nb2CTx and the biological activity of the loaded SeNPs for removing active oxygen and relieving oxidative stress jointly form a composite chemical signal. The simultaneous delivery and synergistic effect of physical and chemical dual signals endow the nano-platform with the ability of efficiently remodeling the functional homeostasis of macrophages, and the nano-platform can be used for treating osteoarthritis.

Genetic markers and a method for the diagnosis or prognosis of degenerative temporomandibular joint osteoarthritis. The method involved detection of a mutation of one or both of SMARCA2 and NOTCH1 or a mutant protein encoded thereby. The detection can be determined from a biological sample, such as saliva, which can be obtained by a non-invasive method. The method involves a random forest-based deep & wide model that is constructed by a machine learning (deep learning) method taken together with the genetic marker mutation information and has excellent diagnostic and predictive abilities with an accuracy of 97%.

The disclosure relates to pharmaceutical compositions and methods of using pharmaceutical compositions comprising effective dosages of an adenoviral-based biological delivery and expression system for use in the treatment or prevention of osteoarthritis in human or mammalian joints by long-term inducible gene expression of human or mammalian interleukin-1 receptor antagonist (IL-1Ra) in synovial cells, comprising a helper-dependent adenoviral vector containing a nucleic acid sequence encoding for human or mammalian interleukin-1 receptor antagonist (IL-1Ra), left and right inverted terminal repeats (L ITR and R ITR), the adenoviral packaging signal and non-viral, non-coding stuffer nucleic acid sequences, wherein the expression of the human or mammalian interleukin-1 receptor antagonist (IL-1Ra) gene within synovial cells is regulated by an inflammation-sensitive promoter.

The invention discloses a pharmaceutical composition for targeted removal of osteoarthritis articular cavity apoptotic cells and application, and belongs to the technical field of biological medicines. The pharmaceutical composition comprises a targeting ligand, an apoptotic cell scavenger and a drug carrier, the targeting ligand is selected from an anti-phosphatidylserine monoclonal antibody or Annexin V protein, and can be specifically combined with phosphatidylserine on the surface of an apoptotic cell; the apoptotic cell scavenger is selected from demethoxycurcumin, andrographolide and the like, and can promote apoptotic cell phagocytosis and inhibit inflammatory factor release; and the drug carrier is hyaluronic acid-polylactic acid copolymer nanoparticles, and has good articular cavity retention property and drug sustained release effect. The pharmaceutical composition disclosed by the invention can be used for accurately targeting apoptotic cells in osteoarthritis articular cavities, efficiently removing the apoptotic cells, inhibiting inflammatory response and delaying cartilage degeneration, is good in biological safety and provides a new effective means for treating osteoarthritis.

This invention relates to a traditional Chinese medicine composition containing celeon bone and its use in improving joint aging and injury. It belongs to the field of traditional Chinese medicine processing technology. The preparation method of celeon bone in the traditional Chinese medicine composition includes the following steps: (1) celeon bone is pulverized to obtain bone powder; (2) the bone powder is added to water and enzymatically hydrolyzed with protease, then inactivated to obtain an enzymatic hydrolysate; (3) microorganisms are added to the enzymatic hydrolysate for fermentation to obtain a fermentation broth; (4) the fermentation broth is inactivated, and solid-liquid separation is performed to obtain a clear liquid; (5) the clear liquid is dried to obtain the final product. The microorganisms include at least one of Bacillus subtilis, Lactobacillus helveticus, Pediococcus pentosaceus, Saccharomyces cerevisiae, Lactobacillus paracasei, and Lactobacillus plantarum. The celeon bone powder prepared by this invention can exert a good anti-inflammatory effect at low doses and can be used to prepare drugs for osteoarthritis caused by joint aging or injury, especially for treating osteoarthritis, rheumatoid arthritis, and other osteoarthritis. It also has good safety and broad application prospects.

Embodiments include a cryogenic device for alleviating pain by cryogenically treating a nerve, the cryogenic device including a handpiece; a needle coupled to a distal end of the handpiece, the needle including a needle lumen, the needle being configured for insertion into a skin of a patient along an insertion axis at a site laterally displaced from a treatment zone proximate to the nerve. The needle is configured to resiliently bend after insertion away from the insertion axis, such that at least a portion of the needle is adapted to traverse a skin layer laterally toward the treatment zone. The device includes a cooling fluid supply tube extending distally into the needle lumen; and a cooling fluid source, wherein the cooling fluid source is coupled to the cooling fluid supply tube to direct cooling fluid into the needle lumen.

This invention belongs to the technical field of bone and joint health products, and provides a composition for improving osteoarthritis, its preparation method, application, and product. The composition comprises, by weight, 1-300 parts mangosteen fruit powder, 1-150 parts eggshell membrane, and 1-250 parts turmeric. The composition of this invention is simple in composition, and through the compounding of mangosteen fruit powder, eggshell membrane, and turmeric in a specific ratio, it shows significant effects in repairing osteoarthritis.

The application discloses a preparation method and application of a multi-morphology fasudil targeted nanomedicine system for diabetes osteoarthritis, and belongs to the technical field of medicines, and comprises the following steps: 1) weighing free fasudil, dissolving the free fasudil in an organic phase solvent, fully stirring and dissolving, and preparing fasudil organic drug stock solution, which is marked as Fas; 2) slowly adding an acid solution to the Fas; the application takes free fasudil as an active drug, constructs Fas@PB-Sr 2+ The composite nano intermediate is subjected to CAP polypeptide targeted modification to obtain multi-morphology targeted nanoparticles. Through a low-temperature hydrothermal process, drug thermal degradation is avoided, square, rod, spherical and porous mesoporous composite morphologies are generated by regulating raw material ratio and reaction conditions, and cartilage specificity enrichment is realized in combination with the CAP polypeptide, multiple pathological damages such as cartilage wear, synovial inflammation and abnormal vascular hyperplasia of diabetes osteoarthritis are improved, joint local drug residence time is prolonged, and systemic drug toxic side effects are reduced.

The application discloses a kind of gait instability evaluation method, device and equipment of knee osteoarthritis patient, comprising: constructing univariate gait event sequence, for multiple predicted steps, out-of-sample multi-step prediction is carried out, the geometric mean absolute prediction error is calculated, and the error-predicted step relationship is logarithmically transformed, and instability proxy index is obtained by adaptive linear growth section regression;Determine the predictability horizon PH in combination with the clinical allowable error threshold, construct the confidence weight using the fitting quality index of linear section, only the instability proxy index is weighted correction, and normalized mapping is carried out, and output instability score and risk classification, simultaneously generate instability profile rolling with time.The present application has strong robustness and interpretability to short-time, noisy clinical data, and the fitting quality index can be fed back to the rolling window update and result output strategy as a reliability control signal to suppress the interference of low-quality data on overall evaluation.

This invention discloses a curcumin liposome microneedle for treating temporomandibular joint osteoarthritis, its preparation method, and its application, belonging to the field of medical biomaterials technology. The preparation method includes the following steps: first, curcumin, cholesterol, and lecithin are evaporated under reduced pressure in a solvent to form a lipid / curcumin membrane; then, phosphate buffer is added for hydration; finally, the hydrated solution is subjected to ice-water bath sonication and centrifugation, and the supernatant is collected to obtain a curcumin liposome solution; methacrylic acid gelatin, water, the curcumin liposome solution, and a photoinitiator are mixed evenly to obtain a casting solution; then, the casting solution is poured into a microneedle mold, and finally, it is cross-linked by light and dried to obtain the final product. This invention constructs a microneedle drug delivery system that directly delivers curcumin liposomes to the dermis through microneedles, overcoming the problem of low blood supply utilization due to the lack of blood vessels in cartilage, improving drug delivery efficacy and comfort, and achieving effective treatment for temporomandibular joint osteoarthritis, with good clinical application prospects.

The application discloses application of UFM1 protein or a coding gene thereof in preparation of a medicine for treating osteoarthritis, and belongs to the technical field of biotechnology.The application proves that knockout of UFM1 accelerates aging of human chondrocytes, overexpression of specific UFM1 protein or a coding gene thereof can relieve aging of human chondrocytes, and specific knockout of UFM1 in a mouse experimental model can accelerate osteoarthritis of the mouse, and injection of UFM1 recombinant protein into a joint cavity of the mouse can relieve osteoarthritis of the mouse.Therefore, the UFM1 protein or the coding gene thereof can be used as a drug target, and has a good prospect in screening and preparation of the medicine for treating osteoarthritis.