11 results about "Zoledronic acid" patented technology

The application discloses a preparation method of CCR2+ gamma delta T cells with anti-tumor activity and migration ability, and belongs to the technical field of cells; the method comprises the following steps: step (1), PBMC of peripheral blood of a healthy person is extracted, inoculated into complete culture medium, and added with zoledronic acid, IL-15 and metformin, and then is cultured in a T25 culture bottle; step (2), complete culture medium is supplemented, the cell concentration is adjusted, and then the cells are transferred into a T75 culture bottle for culture; step (3), complete culture medium is supplemented, the cell concentration is adjusted, glucocorticoid and nicotinamide are added, and then the cells are transferred into a T150 culture bottle for culture; step (4), cells are collected at D6, rinsed with normal saline, resuspended with complete culture medium, the cell concentration is adjusted, and then the cells are inoculated into a T300 culture bottle for culture; and step (5), complete culture medium is supplemented every two days from D8 to D16, the cell concentration is maintained, and then the cells are harvested at D16 to obtain CCR2+ gamma delta T cells.

The invention provides an extraction method of high-performance gamma delta T immune cells and application of the high-performance gamma delta T immune cells in tumors. The method comprises the following steps: firstly, separating mononuclear cells from peripheral blood, carrying out activation induction by using a serum-free culture medium containing zoledronic acid, IL-15 and OKT3, and supplementing a culture medium containing IL-2 according to a specific proportion for multiplication culture from the third day of culture. Through the optimization process, the expression rate of TCR-gamma delta < + > is as high as 96.84%, and gamma delta T cells with high activity are obtained. An in-vivo tumor inhibition experiment shows that the cell has a remarkable growth inhibition effect on liver cancer nude mouse transplanted tumor, the effect is equivalent to that of a positive control drug, and obvious toxic and side effects are not observed. The method is stable in process and clear in component, and a reliable technical basis is provided for clinical transformation of gamma delta T cells in immunotherapy of solid tumors and hematologic tumors.

The application provides a kind of gamma delta T cell culture method and culture medium, belong to T cell culture technical field.In 1640 culture solution is added modified nanoparticle, zoledronic acid, vitamin C, glutamine, wherein, the concentration of the modified nanoparticle is 0.5-1g / L, the concentration of the zoledronic acid is 0.5-1.5 μmol / L, the concentration of the vitamin C is 200-500 μmol / L, the concentration of the glutamine is 0.1-0.2 mmol / L.The culture medium prepared by the application can efficiently stimulate gamma delta T cell expansion and improve its killing activity, and the survival rate of gamma delta T cells is also improved, and the culture method of gamma delta T cells is simple, the cost is low, and has broad application prospect.

The application belongs to the technical field of biotechnology, and particularly relates to a staged precise regulation in vitro expansion culture method of high-activity gamma delta T cells. In the synergistic activation stage, the complex activator containing zoledronic acid, IL-2, IL-15, IL-12 and IL-21 is used to significantly improve the early activation level of the gamma delta T cells; in the dynamic expansion stage, the concentration of metformin is adjusted step by step to support the continuous and efficient proliferation of the cells; in the gradient maturation stage, the combination system of N-acetyl cysteine, TWS119 and DL-alpha tocopheryl acetate is introduced to help maintain the functional activity and antioxidant state of the cells in the later culture stage. The gamma delta T cells with a purity higher than 90% and an expansion multiple more than 100 times can be obtained by using the method to culture for 10 days, and the cells exhibit significant killing activity to tumor cells, are suitable for the adoptive immunotherapy of tumor and infectious diseases, and have good clinical application prospects.

The invention discloses a zoledronic acid hollow nanosphere, a zoledronic acid hollow nanosphere loaded paclitaxel antitumor material and a preparation method and application thereof, the preparation method of the zoledronic acid hollow nanosphere comprises the following steps: dispersing a zeolite imidazole framework 8 in methanol, adding an aqueous solution of zoledronic acid, standing for 60-180 min, centrifuging and cleaning to obtain the zoledronic acid hollow nanosphere loaded paclitaxel antitumor material. The zoledronic acid hollow nanospheres are obtained; the zoledronic acid hollow nanosphere loaded paclitaxel antitumor material can be prepared by loading paclitaxel on the zoledronic acid hollow nanosphere. According to the anti-tumor material, the synergistic anti-tumor effect of the zoledronic acid hollow nanospheres and the paclitaxel can be effectively exerted, and breast cancer cells MCF-7 can be remarkably killed.

The invention relates to bionic chitosan hybrid nanogel for treating prostatic cancer osseous metastasis and a preparation method and application thereof, the hybrid nanogel is prepared by the following steps: carrying out a cross-linking reaction on a chitosan monomer and a cross-linking agent glutaraldehyde, introducing ultra-small ferroferric oxide nanoparticles in the reaction process to form the hybrid nanogel, and then loading zoledronic acid to obtain the bionic chitosan hybrid nanogel for treating prostatic cancer osseous metastasis. And further coating a prostate cancer cell membrane. After entering a tumor-bearing nude mouse body through tail vein injection, the pH response type bionic hybrid nanogel prepared by the invention has the advantages of remarkable prostate cancer bone metastasis inhibition and excellent T1 magnetic resonance imaging performance, and has potential clinical application value.

The invention belongs to the field of biomedical materials, and particularly discloses targeted multi-controlled-release functionalized nanoparticles as well as a preparation method and application thereof. The functionalized nanoparticles are prepared according to the following steps: S1, dissolving PLGA or PLGA and functional compound molecules in acetone, dropwise adding into a TPGS aqueous solution, and volatilizing and centrifuging to obtain drug-loadable PLGA nanoparticles; s2, after the nano-particles react with tannic acid, centrifugal purification is conducted, the nano-particles are resuspended in a TPGS solution for self-assembly, then zoledronic acid is added for a reaction, and the nano-particles are obtained. According to the nanoparticles, bone targeted enrichment is realized by virtue of the affinity of zoledronic acid and bone hydroxyapatite, multiple drug controlled release is realized by virtue of tannic acid crosslinking, therapeutic factors can be stably released for a long time, an osteoporosis pathological microenvironment is corrected, side effects are reduced, and the nanoparticles can be used for treating osteoporosis. The preparation process is simple and efficient, the cost is low, and the reproducibility is good.

This invention provides a nanoformulation capable of enhancing the immune response to in situ tumor vaccines. The nanoformulation comprises zoledronic acid, all-trans retinoic acid, and metal ions. Preferably, the metal ion is a lanthanide metal, such as Gd. 3+ Preferred, zoledronic acid: Gd 3+ The mass ratio of all-trans retinoic acid is 4:1:5. The nano-formulation consists of zoledronic acid and Gd... 3+ Zol / Gd-NPs nanocoagulation polymers were formed by mixing in water. All-trans retinoic acid was dissolved in anhydrous ethanol to prepare albumin-encapsulated nanoparticles (A-NPs), which were then mixed with the Zol / Gd-NPs nanocoagulation polymers and sonicated to obtain the final product. These nanoparticles, on the one hand, can utilize zoledronic acid to eliminate macrophages, thereby reversing the inhibition of intratumoral dendritic cells (DCs) by the tumor immunosuppressive microenvironment; on the other hand, all-trans retinoic acid can significantly increase the expression of MHC-I and tumor antigens in tumor cells, thus ensuring the activation of CD8. + T cells can effectively recognize and kill tumors, generating a systemic anti-tumor immune response.

The application discloses a universal DNT cell public library, a DNT cell preparation and a preparation method thereof, 200 mL of peripheral blood of a healthy adult is separated to obtain mononuclear cells, the mononuclear cells are cultured for 12 days by using zoledronate in vitro, and cell surface markers are detected; when the proportion of CD3+CD4-CD8- is greater than 50%, the cells are frozen to obtain DNT public library cells; when needed, the DNT public library cells are co-cultured with zoledronate for 7 days, and a DNT cell preparation with high purity and high quality can be obtained. The application avoids the ethics and operation burden of repeatedly collecting blood of a donor, reduces production cost and risk of dependence on the donor, has a short preparation period, can support multi-batch production, and has stable quality between batches, and can provide sufficient raw material reserves for clinical trials and patients with diseases.

The application discloses a kind of bovine collagen peptide for increasing bone density and a preparation method thereof, and relates to the technical field of enzyme processing preparation of protein peptide.The application relates to the application of bovine collagen peptide in the field of composition.The composition containing bovine collagen peptide is prepared from the following components in mass fraction:bovine collagen peptide 25-45 parts, calcium citrate 12-28 parts, vitamin D 0.0002-0.0004 parts, extract of rhizoma drynariae 6-14 parts, extract of astragalus 4-9 parts, auxiliary materials 12-38 parts, and zoledronate derivative 0.01-0.05 parts.The application breaks through the limitation of single component and poor synergistic effect in the prior art, and forms a multi-dimensional action system of "inhibiting bone resorption, promoting bone formation and supplementing matrix" by matching bovine collagen peptide, calcium citrate, traditional Chinese medicine extract and modified zoledronate derivative, so that the effect of improving bone density is improved.

Provided in the present invention are a γδT cell culture medium, a culture method and the use. The γδT cell culture medium comprises a basal medium, IL-2, IL-7, IL-15, IL-18, IL-21, zoledronic acid and berberine at a concentration of 0.25-5 μM.