The invention discloses a rolling linear guide friction force dynamic detection device and detection method thereof. The device comprises a bed body, a servo motor, a worktable, a mother lead screw, arolling linear guide, a slide block, an S type tension sensor, a forward limit switch, a reverse limit switch, a motion control card, a control panel and the like; based on the detection method of the device, firstly parameter is set, loading and running-in are carried out on the linear guide to be detected, then dynamic measurement is carried out on friction force and the measured data is analyzed, and finally the test data and result is saved and printed, qualification decision is given and measurement process is finished. The method of the invention can display distribution situation of dynamic measurement data in real time by a computer, and maximum, minimum and mean value thereof are calculated and compared with design standard of corresponding product and then are judged to be qualified or not; compared with the existing static detection method, the method has the advantages of high efficiency, high stability and high reliability.