The invention discloses a method for eliminating optical fiber gyro constant multiplier acceleration effect. The invention comprises the following steps: adding acceleration regulating connection in the output of optical fiber gyro, obtaining acceleration which is perpendicular to the optical fiber gyro sensitive axis plane by the accelerometer in the connection, obtaining the fitting acceleration effect polynomial function of optical fiber gyro constant multiplier from prior test, regulating the output of optical fiber gyro, eliminating the influence of optical fiber gyro constant multiplier acceleration effect; obtaining the fitting acceleration effect polynomial coefficient from the prior turning platform test, selecting a series of testing points with various axial direction valves, which deviate from the turning platform on the turning platform, obtaining constant multipliers and accelerations from every point by test and calculation, obtaining the fitting constant multiplier acceleration effect polynomial coefficient by the least squares technique. The method can effectively eliminate the effect of acceleration to the optical fiber gyro performance, improve resolve precision of the posture, position and others, and improve the dynamic property of the application system at last.