The invention discloses a production technology of microorganism composite microbial inoculum, and relates to the technical field of microorganism compounding agents. The production technology comprises the following steps of S001, identifying strains for production, and purifying or rejuvenating strains having functional changes or polluted by infectious microbes; and S002, before mass productionof the strains, performing laboratory pre-production test on the strains, determining appropriate growth temperature and growth PH of the strains and appropriate components and proportion of fermentation culture mediums, determining appropriate culture time of the strains, and drawing a growth curve of the strains. According to the production technology, the strains are subjected to identification and growing environment test before production, then bevel activation and strain culture are performed to obtain seed liquid and shake flask strains respectively, and finally, through fermentation with a seed jar and fermentation with a fermenter, the microorganism composite microbial inoculum is prepared. The microorganism composite microbial inoculum is simple in preparation process and low inpreparation cost, the quality of the microorganism composite microbial inoculum is improved, and the meat quality of livestock is effectively guaranteed.