Improved DY-IV medium suitable for growth of Dinobryon, and preparation method and application thereof
A technology of DY-IV and medium, which is applied in the field of improving DY-IV medium and preparation, and can solve problems such as difficult growth of algae cells
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Embodiment 1
[0034] Embodiment 1 (compared with DY-IV medium)
[0035] The Corocystis collected from Pipahai, Ningwu, Shanxi was cultured artificially in DY-IV medium and improved DY-IV medium.
[0036] A. Cultivation experiment of Cynocystis in DY-IV medium:
[0037] Prepare 1L of DY-IV medium:
[0038] Medium composition ratio:
[0039] CaCl 2 .2H 2 O 0.02g / L, MgSO 4 .7H 2 O 0.074g / L, NaNO 3 0.02g / L, Na 2 HPO 4 0.0066g / L, NaSiO 3 .9H 2 O 0.015g / L, NH 4 NO 3 0.01g / L, KCl 0.01g / L, MES buffer 0.25g / L, metal solution 1ml / L, vitamin solution 1ml / L.
[0040] Components and content thereof of the metal solution: MnCl 2 0.2g / L, ZnSO 4 0.04g / L, NaMoO 4 0.02g / L, CoCl 2 0.008g / L, Na 2 EDTA.2H 2 O 8g / L, FeCl 3 0.7g / L,H 3 BO 3 0.8g / L.
[0041] The components and contents of the vitamin solution: vitamin B1 0.2g / L, vitamin B7 0.0005g / L, vitamin B120.0005g / L.
[0042] Culture medium preparation method:
[0043] 1) Add 900mL of distilled water to a sterilized 1L container,...
Embodiment 2
[0071] Embodiment 2 (compared with DY-IV medium)
[0072] The Corymocystis collected in Yunzhu Lake, Yushe, Shanxi Province is artificially cultivated, and the culture medium preparation and cultivation method are the same as in Example 1, and the specific implementation results are as follows Figure 5 , 6 , 7 and 8.
[0073] The corymocystis cell number in embodiment 1 and embodiment 2 is summed up, and the result is as follows Figure 9 and Figure 10 Shown: grow in the Conecystis cells in DY-IV substratum without proliferation, but the growth rate of Coneocystis Ningwu cells and Conecystis yushe cells in the improved DY-IV medium of the present invention is fast, algae The sustainable growth time of the cells is long, which shows that the improved DY-IV medium of the present invention is most suitable for the growth of Cynomoria cells.
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