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Improved DY-IV medium suitable for growth of Dinobryon, and preparation method and application thereof

A technology of DY-IV and medium, which is applied in the field of improving DY-IV medium and preparation, and can solve problems such as difficult growth of algae cells

Active Publication Date: 2018-08-03
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, it is difficult to grow algae cells cultured in DY-IV medium, and it is impossible to artificially cultivate the Corymocystis collected in the field in the laboratory

Method used

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  • Improved DY-IV medium suitable for growth of Dinobryon, and preparation method and application thereof
  • Improved DY-IV medium suitable for growth of Dinobryon, and preparation method and application thereof
  • Improved DY-IV medium suitable for growth of Dinobryon, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1 (compared with DY-IV medium)

[0035] The Corocystis collected from Pipahai, Ningwu, Shanxi was cultured artificially in DY-IV medium and improved DY-IV medium.

[0036] A. Cultivation experiment of Cynocystis in DY-IV medium:

[0037] Prepare 1L of DY-IV medium:

[0038] Medium composition ratio:

[0039] CaCl 2 .2H 2 O 0.02g / L, MgSO 4 .7H 2 O 0.074g / L, NaNO 3 0.02g / L, Na 2 HPO 4 0.0066g / L, NaSiO 3 .9H 2 O 0.015g / L, NH 4 NO 3 0.01g / L, KCl 0.01g / L, MES buffer 0.25g / L, metal solution 1ml / L, vitamin solution 1ml / L.

[0040] Components and content thereof of the metal solution: MnCl 2 0.2g / L, ZnSO 4 0.04g / L, NaMoO 4 0.02g / L, CoCl 2 0.008g / L, Na 2 EDTA.2H 2 O 8g / L, FeCl 3 0.7g / L,H 3 BO 3 0.8g / L.

[0041] The components and contents of the vitamin solution: vitamin B1 0.2g / L, vitamin B7 0.0005g / L, vitamin B120.0005g / L.

[0042] Culture medium preparation method:

[0043] 1) Add 900mL of distilled water to a sterilized 1L container,...

Embodiment 2

[0071] Embodiment 2 (compared with DY-IV medium)

[0072] The Corymocystis collected in Yunzhu Lake, Yushe, Shanxi Province is artificially cultivated, and the culture medium preparation and cultivation method are the same as in Example 1, and the specific implementation results are as follows Figure 5 , 6 , 7 and 8.

[0073] The corymocystis cell number in embodiment 1 and embodiment 2 is summed up, and the result is as follows Figure 9 and Figure 10 Shown: grow in the Conecystis cells in DY-IV substratum without proliferation, but the growth rate of Coneocystis Ningwu cells and Conecystis yushe cells in the improved DY-IV medium of the present invention is fast, algae The sustainable growth time of the cells is long, which shows that the improved DY-IV medium of the present invention is most suitable for the growth of Cynomoria cells.

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Abstract

The invention discloses an improved DY-IV medium suitable for the growth of Dinobryon. The main components of the modified DY-IV medium of Dinobryon include CaCl2.2H2O, MgSO4.7H2O, Na2HPO4, NaNO3, NaSiO3.9H2O, NH4Cl, KCl, MES buffer, a metal solution, a vitamin solution and distilled water. The medium can be used to perform laboratory artificial culture on fresh Dinobryon collected in the field. The problem that subsequent life history observation and molecular systematic analysis cannot be performed due to the previous Dinobryon study is only limited to fixed study of the collection in the field and the addition of a Lugol's iodine solution and the Dinobryon cannot be artificially cultured is solved in the invention.

Description

technical field [0001] The invention relates to an algae culture medium, in particular to an improved DY-IV medium suitable for the growth of Corymocystis and its preparation method and application. Background technique [0002] The genus Chrysocystis belongs to the Chrysophyta, which is widely distributed in fresh water bodies and plays an important role in indicating oligotrophic water bodies. Because there is no cell wall to surround, the protoplast is easily degraded and destroyed, the species identification is difficult, and the classification system is complex and changeable. At present, there is no report on the laboratory culture of Corymocystis, and the research on it is limited to collecting samples in the field and fixing them with Lugol's iodine solution for morphological observation. [0003] Initially, DY-IV medium was used for the cultivation of Cynocystis, and the formula of DY-IV medium was CaCl 2 .2H 2 O0.02g / L, MgSO 4 .7H 2 O 0.074g / L, Na 2 HPO 4 0...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 姜小蝶冯佳谢树莲吕俊平刘琪南芳茹
Owner SHANXI UNIV
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