59results about How to "Realize artificial cultivation" patented technology

An artificial cultivation method of morchella in windy and sandy areas includes following steps: step 1, sequentially preparing mother seeds, raw seeds and cultivation seeds; step 2, building a sunlight greenhouse and a greenhouse for cultivation; step 3, preparing a land: combining with land ploughing before sowing, applying a rotten matrix, and dividing ridge areas; step 4, sowing: uniformly scattering the cultivation seeds to space behind ridge faces, furrowing along lines, and earthing; step 5, performing field management; step 6, harvesting: timely harvesting when cap veins are observed clearly to obtain mature morchella. Relying on resources of wild morchella in the windy and sandy areas of the North, through methods of tissue separation, spore culture or hybridization integration, a strain suitable for local growth is cultivated and domesticated after strict screening and fruiting experiment; according to a scientific formula, a mode of three-stage seed preparation is adopted, facilities like the sunlight greenhouse and the plastic greenhouse are utilized to increase shade and a micro spray irrigation system, and morchella is obtained by cultivation by artificially controlling environment conditions like temperature, humidity, illumination and ventilation, so that a technical gap is filled in.

The invention relates to a method for artificially culturing fuscous dictyostelium boletes in mushroom houses, greenhouses, field and nursery. The method is characterized in that firstly bolete rods are cultured, then (1) the bolete rods are earthed up to culture mushroom in mushroom houses and greenhouses, fuscous dictyostelium boletes substance can be grown and developed to mature after 10-15 days of earthing; (2) the bolete rods are debagged and earthed up to culture mushroom in mushroom houses and greenhouses, and fuscous dictyostelium boletes substance can be grown and developed to mature after 20-25 days of earthing; (3) solid bolete strains are inoculated at the roots of tropical cash crops and flower plants planted in fields to culture mushroom, and fuscous dictyostelium boletes substance can be grown and developed to mature after 30-60 days of inoculation; artificial culture of fuscous dictyostelium boletes and the industrialization production of the fuscous dictyostelium boletes can be fast realized. The culture method is simple and convenient, the culture period is short and the fruiting is regular and the yield is high.

The invention relates to a cultivation and domestication method of wild collybia albuminosa. The method mainly comprises the following steps that 1, mother strain separation is conducted, delicate body cell induced callus tissue is directly extracted from a growing collybia albuminosa nutrition body, hyphae are cultivated gradually, and pure white and strong hypha bodies are selected to serve as mother strains; 2, preparation of mother strain culture media is conducted; 3, the hyphae of which the growth vigor is healthy and strong after expanding propagation is conducted on the main strains are inoculated into the mother strain culture media, culture is conducted in a constant temperature incubator with the temperature of 25 DEG C till the hyphae are overgrown in the culture media, and strong hyphae are selected to serve as original strains; 4, preparation of original strain culture media is conducted; 5, the original strains are inoculated into a cultivation bottle containing the original stain culture media, culture is conducted in the constant temperature incubator with the temperature of 25 DEG C, and cultivated species are obtained. According to the cultivation and domestication method of the wild collybia albuminosa, artificial cultivation of the collybia albuminosa on the condition that termites do not exist can be achieved, fruiting is fast, and the yield is stable.

The invention relates to a technical field of cultivating edible fungi, in particular to a method for artificially cultivating suillus luteus garden. The method for artificially cultivating the suillus luteus comprises the following steps: strains are separately cultivated; the strains are propagated; aseptic seedlings are raised; mycorrhizal seedlings are raised and mushroom is cultivated. By using synthetic suillus luteus wetland pinus mycorrhiza to densely plant in a sterile garden with an interval of 10-50cm, the invention realizes the artificial cultivation and achieves the goal of industrial production of the suillus luteus. The produced suillus luteus has the same morphological characteristics with natural suillus luteus.

The invention discloses a white grassland mushroom domestication method and cultivation process, wherein the white grassland mushroom original breed is obtained through the steps of, selecting different culture medium and culture condition, making female seeds, female seeds culturing, and making original breed as a domestication procedure, and then the cultivation is realized through controlling the culture condition of temperature and humidity.

The invention provides a culture method for a tricholoma matsutake liquid strain. The culture method includes the steps that a culture medium for the tricholoma matsutake liquid strain is prepared, the culture medium for the tricholoma matsutake liquid strain is inoculated with a tricholoma matsutake stock seed for sealing culture, and after hyphae of the tricholoma matsutake stock seed germinate, the culture medium for the tricholoma matsutake liquid strain is shaken once every day until the tricholoma matsutake liquid strain is obtained. The culture method for the tricholoma matsutake liquid strain is easy to operate, cultured richoloma matsutake liquid strain hyphae grow fast and are contaminated by other strains little, the hypha output rate is high, and cost is low.

The invention relates to a new strain, a culturing method and an application thereof and specifically relates to the new strain of isaria tenuipes, the culturing method and the application thereof. The new strain of isaria tenuipes is Isaria tenuipes HMGIM-W150712, and the collection number is CCTCC No: M2017430. The method comprises the following steps: separating the stock culture, purifying the stock culture and then preparing a liquid stock; inoculating the liquid stock into a culture medium for culturing; and managing the culturing. Through repeated test and research, the inventor finds the culture medium and the culturing method suitable for the strain of isaria tenuipes provided by the invention; the artificial cultivation for the isaria tenuipes is realized; and after the artificial cultivation, the length of the sporocarp thereof is greatly longer than the length of wild strain, the biological conversion rate is about 33.36% and the fruiting period is short.

The present invention provides a method for cultivating Polyporus sclerotium capable of efficiently multiplying the sclerotia of Polyporus umbilicalis in a short period of time. A method for cultivating Polyporus, characterized in that Polyporus is symbiosised with Armillaria which has low phytopathogenicity.

The invention discloses a method for artificially cultivating litchi chinensis bacteria. The method comprises the following steps: (1) carrying out termite mound building; (2) carrying out termite propagation; (3) carrying out mycelium inoculation; and (4) carrying out daily management. The invention further discloses a method for preparing a litchi chinensis wood crude extract. The method comprises the steps of crushing litchi chinensis wood, adding 0.1mol/L to 0.3mol/L of oxalic acid into the crushed litchi chinensis wood, carrying out treatment for 2.5 to 3.5 hours at the temperature of 98 DEG C to 104 DEG C, washing litchi chinensis wood extracted residues by water, and then, diluting the residues by 3 to 5 times, thereby obtaining the litchi chinensis wood crude extract. Compared with the prior art, the method has the beneficial effects that the migration of termites can be effectively prevented, the propagation of the termites and the growth of the litchi chinensis bacteria are promoted, the wild growth conditions of the litchi chinensis bacteria can be simulated to the maximum, the wild taste of the litchi chinensis bacteria is reserved, the good growth and high yield of the litchi chinensis bacteria are guaranteed, the cost is low, the artificial cultivation of the litchi chinensis bacteria is achieved, and the seasonal restriction of growth of the litchi chinensis bacteria is broken through, so that the method has a great economic value.

The invention discloses a cultivation method of an oudemansiella radicata strain. According to the cultivation method, an edible fungus cultivation device is used for cultivating the oudemansiella radicata strain, the edible fungus cultivation device provides proper cultivation conditions for the oudemansiella radicata strain through a vibration cultivation mechanism, and manual cultivation is replaced with mechanical equipment cultivation, so that the culture efficiency and accuracy of the oudemansiella radicata strain are effectively improved; a porous culture medium carrier is prepared in the strain culture process, and a scientific and reasonable strain culture medium is used in cooperation, so that artificial culture of the oudemansiella radicata strain is achieved, various types of bioactive substances are contained in strain culture, a promoting effect on formation of oudemansiella radicata mycelium primordia and growth of fruiting body mycelia, and oudemansiella radicata cultured by the cultured oudemansiella radicata strain has the advantages of thick stipe, bright color, fleshy flesh, high environmental adaptability, high spawn running speed, short production period and stable yield.

The invention discloses a method for seed germination and seedling culture of tutcheria microcarpa. The method comprises the following steps: (1) collecting fresh and ripe fruits and covering the fruits by using a wet tissue with the relative humidity of 70%-90%; placing the fruits into a closed container and carrying out after-ripening treatment under an indoor dark condition; then shucking to obtain seeds; immersing the seeds by using a potassium permanganate solution with the mass percentage of 0.1%-0.3%; and drying in the shade for future use; and (2) spraying a fenaminosulf solution which is diluted for 2,000-3,000 times on a base material and standing for three days; watering clean water thoroughly on the fourth day; scattering the immersed seeds into the base material and covering the base material with a layer of a shading net; and watering or spraying every day for preserving moisture, wherein the base material comprises the following components in mass ratio of ceramsite to perlite to vermiculite to river sand to peat soil being 1 to 2 to 2 to 2 to 4 and the seed germination temperature is 10-29 DEG C. According to the method, the emergence rate of the tutcheria microcarpa is improved through the after-ripening treatment and dormancy breaking, so that the seedling culture needed by artificial cultivation is achieved and artificial cultivation breeding can be carried out by seeding.

The invention discloses a culture method of triplophysa anterodorsalis fry. The method comprises the following steps of (1) temporary culture of the fry, (2) opening of the fry, (3) the feeding stage of live bait, (4) the feeding stage of tubificidae, (5) the eating training stage, (6) the fry culture stage and (7) disease control. Accordingly, by means of precise control of the seven steps, manual culture of the triplophysa anterodorsalis fry is successfully achieved for the first time in China, the fry survival rate reaches 92% or above, the method is simple, and popularization is easy.

The invention discloses a method for separating, cultivating and identifying a skin epithelial cell of a giant salamander. According to the method, a skin tissue is acquired from the tail part of the giant salamander, a tissue explant method is used to separate to acquire the primary skin epithelial cell of the giant salamander, the primary skin epithelial cell of the giant salamander is subjected to primary culture and subculture in a specific culture solution (the ingredients comprise DMEM/F12 (60%), 15% of FBS, 5 mug/mL of insulin, and 10 ng/mL of KGF) at the temperature of 28 DEG C, and specific genes (K5, K10 and P63) of the acquired skin epithelial cell of the giant salamander are expressed and identified through RT-PCR (Reverse Transcription-Polymerase Chain Reaction). The homologous design primer of the gene sequence of the related species is used to conduct PCR amplification on the genes K5, K10 and P63 of the giant salamander to acquire the gene sequences of K5, K10 and P63 of the giant salamander for the first time. The provided method for separating, cultivating and identifying the skin epithelial cell of the giant salamander lays foundations for the deep study of the skin of the giant salamander as well as the skin regeneration, trauma repair and tissue engineering skin construction with the help of the skin epithelial cell of the giant salamander.

The artificial culture of hair weeds seedlings includes the following steps: selecting thallus of hain weeds, cleaning with distilled water, moistening with ethyl alcohol, repeatedly washing with sterile water, placing the cleaned thallus of hair weeds in the liquid culture medium to make photosynthesis and restoration, pulverizing the thallus of the hair weeds so as to obtain free unicell and filament, cultivating the unicell and filament in liquid culture medium to obtain the filament with thickened sheath, taking out the filament and transferring it on the surface of agar culture plates, then quantitatively watering the surface of culture medium at regular time every day.

The invention provides a culture medium used for batrachospermaceae and application of the culture medium. The culture medium used for the batrachospermaceae is mainly prepared from the main components including NaNO3, K2HPO4, KH2PO4, MgSO4.7H2O, CaCl2, NaCl, a PIV metal solution, a vitamin B12 solution, a vitamin H solution, a vitamin B1 solution, a penicillin-streptomycin solution and distilled water. By utilizing the culture medium, fresh batrachospermaceae algae collected in the wild are artificially cultured in a laboratory, and an indoor living state of the batrachospermaceae is kept. With the adoption of the culture medium provided by the invention, the difficulties that previous batrachospermaceae researches are only limited to wild collection and growth and the batrachospermaceae cannot be artificially cultured, so that subsequent life history observation, molecular biological analysis can not be performed and the like are overcome.

The invention relates to the technical field of wild vegetable cultivation, in particular to a cultivation method for portulaca oleracea. The cultivation method for portulaca oleracea includes the steps of seed treatment, seedling growing compartment surface preparation, seedling culture, seedling management, seedling transplanting, and portulaca oleracea harvesting. By sowing portulaca oleracea seeds, culturing the seedlings and then performing transplanting and field planting, the artificial cultivation of wild portulaca oleracea is achieved, the planting yield of the portulaca oleracea is expanded, and the portulaca oleracea appears on a dinner table for the public like other common vegetables. At the same time, the portulaca oleracea seeds undergo accelerating germination treatment through accelerating germination liquid, the germination rate of the portulaca oleracea seeds and the disease resistance of portulaca oleracea plants at a later period are effectively improved, and the growth vigor of the portulaca oleracea plants is improved; no pesticide is applied during the growth of the portulaca oleracea, the fertilization is based on farmyard manure, the medicinal value and edible value of the cultured portulaca oleracea are equivalent to those of wild portulaca oleracea, and the cultured portulaca oleracea is green and healthy.

The invention discloses an artificial cultivation method of red-stripe lysmata vittata larvae. The artificial cultivation method comprises following steps: collection of larvae; density and environmental requirements of larva cultivation; recirculating aquaculture prior to larval metamorphosis and cultivation of larvae. The artificial cultivation method of red-stripe lysmata vittata larvae has following beneficial effects: artificial cultivation is achieved for the first time by adoption of artificial cultivation technology so that survival rate and metamorphosis rate of zoea are greatly increased; and all of these provide quantity and quality assurance for red-stripe lysmata vittata in an aquatic market .

The invention relates to plant tissue culture, and discloses a tissue culture method of rosa graciliflora. The tissue culture method comprises the following steps: (1) axillary bud induction: taking an annual stem section with a stem node of the rosa graciliflora as an explant, and inoculating the explant into a primary culture medium for axillary bud induction culture; (2) cluster bud induction and proliferation: transplanting a germinated axillary bud into a proliferation culture medium for cluster bud differentiation and proliferation; and (3) rooting culture: transferring a seedling obtained in the step (2) into a rooting culture medium, and performing rooting culture for 14-30 days. According to the culture method disclosed by the invention, artificial culture of the rosa graciliflorais realized for the first time, the seed emergence rate, the axillary bud germination rate, the proliferation coefficient and the rooting rate are high, massive rapid propagation of the rosa graciliflora is facilitated, and the tissue culture method has good application prospects.

The invention provides a tricholoma matsutake stock culture medium. The tricholoma matsutake stock culture medium is prepared from the following components in parts by weight: 2-5 parts of sawdust, 1-3 parts of cottonseed hulls, 1-3 parts of corncobs, 1-3 parts of lotus seed hulls, 1-3 parts of bran, 0.05-0.1 part of lime, 0.05-0.15 part of gypsum, 0.3-0.7 part of plant ash and 0.2 part of rice husks. After tricholoma matsutake stock seeds are obtained, artificial cultivation of tricholoma matsutake can be achieved.