Planting method of polyporus
A cultivation method, the technology of Polyporus, which is applied in botany equipment and methods, horticulture, fungi, etc., can solve the problems of insufficient efficiency and cost, long cultivation time, uneasy supply and quality stability, etc., and achieve the realization of Artificial cultivation, good effect of efficiency
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Embodiment 1
[0028] Production of seed material
[0029] A log (Ф9 cm×30 cm) of a broad-leaved tree (Quercus pagoda) was immersed in a 1 / 2 concentration PDB (potato dextrose broth) medium for 1 day. This log was transferred to a heat-resistant plastic bottle (2 L), and 150 mL of a PDA (potato dextrose agar) medium was further added thereto, and autoclaved at 121° C. for 1 hour in an autoclave. After cooling, the colonies of the following four types of Armillaria genus cultured on PDA plates in advance for 7 days were aseptically cut out to a size of 0.5 cm square, and 4 to 5 colonies were each inoculated into the above-mentioned bottles. They were cultured in the dark at 25° C. for about 4 months to produce seed materials in which Armillaria genus flourished.
[0030] The Lemonella species used
[0031] Highly phytopathogenic strains:
[0032] Armillaria mellea (Armillaria mellea; harvested near Rukatsu Bridge, Nasushiobara Town, Tochigi Prefecture in November 2002)
[0033] Armill...
Embodiment 2
[0055] Production of seed material
[0056] A seed material was produced in the same manner as in Example 1 using Armillaria gallica as the species of Armillaria.
[0057] Manufacture of the fungus bed
[0058] Dig a pit with a length of 60 cm x a width of 50 cm x a depth of 30 cm in the field, lay a layer of dead leaves of a broad-leaved tree at the bottom, and place Armillaria mellea bacteria on the dead leaves. The sclerotium of Polyporus sclerotium about 2 cm in size was sown, and a log (Ф7 cm×45 cm) of a broad-leaved tree (Quercus pagoda) was placed on it. Cover its surroundings with a layer of humus, and then fill the upper part with about 10cm of soil. Add water regularly to keep the soil moist all the time, and cultivate for about 7 months.
[0059] It was found that the sclerotia of the cultured Polyporus umbilicali and Armillaria mellifera were symbiotic and multiplied.
Embodiment 3
[0061] Production of seed material
[0062] A seed material was produced in the same manner as in Example 1, using Armillaria jajali and Armillaria gallica as Armillaria species.
[0063] Manufacture of the fungus bed
[0064] Lay the dead leaves of one deck broad-leaved tree at the bottom of polypropylene box (35cm * 72cm * 35cm), put Armillaria armillaria bacterial material on the dead leaves. The sclerotium of Polyporus sclerotium about 2 cm in size was sown, and a log (Ф7 cm×45 cm) of a broad-leaved tree (Quercus pagoda) was placed on it. Cover its surroundings with a 1:1 mixture of humus soil and sand, and then fill the upper part with about 10cm of soil. At a temperature of 25° C. and a humidity of 65%, they were cultured for about 7 months.
[0065] It was found that the sclerotia of the cultured Polyporus umbilicali and Armillaria mellifera were symbiotic and multiplied.
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