495 results about "Culture plates" patented technology

The invention discloses tissue cell activity detection equipment, and relates to the technical field of cell activity detection.The tissue cell activity detection equipment comprises a detection box, a box door is hinged to the outer wall of one side of the detection box, and a culture plate is slidably connected to the bottom of an inner cavity of the detection box; at least four sliding grooves are symmetrically formed in the inner wall of the detection box, a detection person pulls an operation handle to promote a connecting base to slide along the surface of a limiting column, a gear is meshed with a plurality of racks to promote the gear to drive a liquid storage tank to rotate by 180 degrees in a reciprocating mode, and then cell suspension is continuously shunted through a plurality of stop levers. Therefore, the liquid storage tank can be continuously rotated when the cell suspension is dripped, so that the cell suspension can be prevented from being in a static state for a long time to generate a precipitation phenomenon, the cell density distribution of the cell suspension in the holes in the culture plate can be ensured to be uniform, the accuracy and the reliability of a detection result can be improved, and the detection efficiency is improved. The quality of detection data can be improved, and meanwhile, the time and the cost of repeated detection due to inaccurate detection results are reduced.

The invention discloses a portable digital constant-temperature incubator which comprises an incubator body shell, an upper cover and an inner container. A heat preservation module is filled between the box body shell and the inner container; a heating module is arranged on the outer side of the inner container, the incubator is provided with a temperature control module, and the temperature control module comprises a controller, a circuit board, a temperature sensor and a temperature displayer. The temperature sensor is installed on the outer wall of the inner container. The temperature control module realizes temperature monitoring, feedback and temperature control; a culture tray and a detection bag bracket are fixed in the inner container; the temperature control module is electrically connected with a power supply module, and the power supply module provides electric energy for the portable digital constant-temperature incubator. Therefore, the invention provides the portable digital constant-temperature incubator which is light, good in temperature control and capable of continuously working in the field.

The utility model discloses a tissue culture flower rapid propagation induction device, and belongs to the technical field of flower planting. Comprising a bottom rod and multiple sets of culture plates, grooves are formed in the culture plates, limiting grooves are formed in the two sides of the grooves, the culture plates are slidably connected with a sliding plate through the limiting grooves, multiple sets of limiting pipes are evenly installed on the upper surface of the sliding plate, and a limiting assembly is installed in the center of the side face of the sliding plate. The tissue culture flower rapid propagation induction device has the beneficial effects that through arrangement of a limiting pipe, the limiting pipe provides limiting for a culture bottle, the situation that the culture bottle is touched and consequently the culture bottle falls off and is damaged is avoided, through arrangement of a limiting assembly, a sliding plate is conveniently limited, and when the sliding plate does not need to be pulled out, the sliding plate is fixed, so that the tissue culture flower rapid propagation induction device is convenient to use. Through sliding connection between the sliding plates and the culture plates, the sliding plates can be conveniently pulled out, the culture plates on the upper layer cannot hinder taking and placing of the culture bottles, and then the culture bottles can be conveniently taken and placed.

The present invention refers to a method for coating a collagen like protein onto a surface of a polymer-scaffold comprising or consisting the following steps: a) plasma-treating the surface of the polymer-scaffold, b) providing a collagen like protein in a solvent, to obtain a collagen like protein-containing solution, c) bringing the plasma treated polymer-scaffold and the collagen like protein containing solution into contact followed by an optionally rinsing, d) removing the solvent and thereby obtaining a collagen like protein coated polymer-scaffold. Furthermore, the present invention refers to an article obtained by the method of the present invention. Moreover, the present invention refers to the use of the article according to the invention for wound healing and bone repair as well as for flask-coating and plate-coating in cell culture.

The present invention refers to a method for coating a collagen like protein onto a surface of a polymer-scaffold comprising or consisting the following steps: a) plasma-treating the surface of the polymer-scaffold, b) providing a collagen like protein in a solvent, to obtain a collagen like protein-containing solution, c) bringing the plasma treated polymer-scaffold and the collagen like protein containing solution into contact followed by an optionally rinsing, d) removing the solvent and thereby obtaining a collagen like protein coated polymer-scaffold. Furthermore, the present invention refers to an article obtained by the method of the present invention. Moreover, the present invention refers to the use of the article according to the invention for wound healing and bone repair as well as for flask-coating and plate-coating in cell culture.

The invention provides a method for producing cell culture meat through 3D scaffold-free co-culture of pseudosciaena crocea stem cell spheres, and belongs to the technical field of preparation of cell culture meat. A method for producing cell culture meat by 3D scaffold-free co-culture of pseudosciaena crocea stem cell spheres comprises the following steps: mixing and inoculating pseudosciaena crocea myoblasts and pseudosciaena crocea precursor adipocytes into a cell culture plate subjected to anti-adhesion treatment for proliferation culture, spontaneously gathering to form cell spheres, and carrying out differentiation culture to obtain the cell culture meat. According to the co-culture mode provided by the invention, high-efficiency myogenic differentiation and adipogenic differentiation of myoblasts and precursor adipocytes of the large yellow croakers can be realized through proliferation culture and differentiation culture, and an in-vivo microenvironment can be effectively simulated, so that cultured meat similar to a traditional meat product in texture and flavor is produced.

The utility model provides a temperature control behavioral detection device for small-model organisms. The device mainly comprises three systems, namely a behavioral monitoring system, a temperature control system and an optical genetic control system. Wherein the behavioral monitoring system consists of a porous culture plate and an infrared camera device, and is used for recording behaviors of small-model organisms; the temperature control system adopts a 24-hole, 48-hole or 96-hole thermosensitive metal plate structure, the temperature is accurately controlled through a programmable multi-section temperature controller, and a water cooling system is arranged to realize rapid heat dissipation; the optical genetic control system uses an LED lamp with a specific wavelength to realize accurate regulation and control of neurons. According to the device, through the structural design that the thermosensitive metal plate is tightly attached to the culture plate, accurate regulation and control of the culture environment temperature are achieved, and the technical problems that a traditional incubator is slow in temperature response and large in fluctuation are solved. The device is compact in structure, accurate in temperature control and convenient to operate, and is particularly suitable for behavioral research of small model organisms such as drosophila melanogaster.

The invention discloses a high-flux organoid culture liquid core device which comprises an upper cover module, a transwell module and a porous culture plate, more than one culture hole is formed in the porous culture plate, a liquid inlet hole and a liquid outlet hole are formed in the bottom of each culture hole, at least one culture liquid circulation channel is formed in the porous culture plate, and the culture liquid circulation channels are communicated with the culture holes. The culture solution circulation channel comprises an injection port, an input channel, a discharge channel and a discharge port, the injection port is communicated with the input channel, and the discharge channel is communicated with the discharge port; the porous culture plate comprises a plurality of culture holes and a plurality of culture solution circulation channels, each culture solution circulation channel is communicated with an injection port and a discharge port of at least one culture hole, the transwell module comprises more than one transwell chamber, the transwell chambers are matched with the culture holes and placed on the culture holes, and the upper cover module is used for sealing and covering the porous culture plate. According to the liquid core device, a traditional culture plate and a micro-fluidic technology are combined, high-throughput culture of the organoid is achieved, and the homogeneity of the organoid culture tissue is improved due to the fact that a parallel multi-group organoid culture mode is adopted.

The invention discloses a culture method of high-activity human urine-derived stem cells, which relates to the technical field of biological medicine, and is technically characterized by comprising the following steps: collecting urine samples of pregnant and lying-in women, coating a culture plate with gelatin, inducing adherence, performing passage amplification, performing immunofluorescence identification, performing function detection and other optimized conditions, and separating and culturing the stem cells. Compared with urine-derived stem cells of normal adults, the stem cells derived from pregnant women have stronger activity, higher dryness and faster multiplication capacity. The invention provides a novel and superior cell source for tissue engineering and regenerative medicine.

The invention relates to the technical field of preparation of organoids, and discloses a method for regulating and controlling human induced pluripotent stem cells to differentiate into kidney organoids, which comprises the following steps: resuspending a cell cluster formed by human induced pluripotent stem cells by using a basic differentiation culture solution containing a GSK-3beta inhibitor, inoculating the cell cluster into an ultralow adsorption cell culture plate, and starting to culture, marking the time as the 0th hour of differentiation; and after culturing for 70-76 hours, replacing the basic differentiation culture solution containing the GSK-3beta inhibitor with a basic differentiation culture solution containing a Knockout TM serum substitute (KSR), and continuing to carry out differentiation culture. By adopting the method disclosed by the invention, the human induced pluripotent stem cells can be efficiently, quickly, simply, conveniently and stably subjected to directional induced differentiation into kidney organs containing podocytes, renal tubules and other structures, and the time for constructing a kidney in-vitro model can be shortened, so that the research on kidney diseases, kidney development and the like can be greatly assisted; and a powerful platform is provided for kidney toxicity assessment.

The embodiment of the utility model discloses cell culture detection equipment. The equipment comprises a control device, a base, and a pipetting device, a culture area, a functional area and a reagent area which are arranged on the base. The control device is electrically connected with the pipetting device; the culture area extends along a first direction, a culture plate is arranged in the culture area, and the culture plate is provided with a plurality of culture cavities for cell culture and / or chemical analysis; the reagent area and the culture area are adjacently arranged, the reagent area, the functional area and the culture area are adjacently arranged in the second direction, the second direction is perpendicular to the first direction, and the reagent area is used for containing a container for containing reagents. The cell culture detection equipment has the advantages of high automation degree and small size. Complex operation is integrated into the cell culture and detection equipment, meanwhile, operation which is long in occupied time and low in intelligent requirement is removed, the processing efficiency of the cell culture and detection equipment can be improved, and industrial production of cells or organoids is met.

The utility model discloses a cell culture box for avoiding contamination, wherein the rotating mechanism comprises a motor, a ratchet and a first connecting rod, the motor is fixedly mounted on the bottom of the box, the output end of the motor passes through the bottom of the box and is fixedly connected with a second connecting rod, the first connecting rod is rotatably connected with the second connecting rod, the top of the first connecting rod is rotatably connected with a third connecting rod, the top of the ratchet is fixedly connected to the bottom of a culture plate, and the bottom of the ratchet is fixedly connected with a rotating shaft, and relates to the technical field of cell culture devices, the second connecting rod is rotated by the rotation of the motor, and the ratchet is driven to rotate in coordination with the first connecting rod, the third connecting rod and a pushing component, so that the culture plate and the culture dish in the box are rotated to a certain angle, so as to bring the object to be observed closer for better observation, and at the same time, the angle can be better adjusted to add the required solution to the culture dish that needs to be added with liquid.

The invention relates to the technical field of organoid construction, and discloses a heart organoid construction method which comprises the following steps: preparing a culture medium I containing CD lipid, ITS-X, L-AA2P, alpha-MTG, insulin, FGF2 and a GSK-3beta inhibitor; preparing a culture medium II containing CD lipid, ITS-X, L-AA2P, alpha-MTG, insulin, FGF2 (fibroblast growth factor 2) and VEGFA (vascular endothelial growth factor A); the method comprises the following steps: digesting a human induced pluripotent stem cell mass, suspending the human induced pluripotent stem cell mass in a culture medium I, inoculating the human induced pluripotent stem cell mass into an ultra-low adsorption cell culture plate with a U-shaped hole bottom, culturing for 36-40 hours, removing the culture medium I, adding a culture medium II, and culturing until a heart organ is formed. In the method provided by the invention, two culture media are sequentially used for performing two-stage differentiation culture, namely, the heart organoid can be constructed from the human induced pluripotent stem cells dispersed after digestion, so that the construction process of the heart organoid is simplified, the construction time is shortened, and the operation difficulty is reduced.

The invention relates to an efficient screening method and application of an influenza virus candidate vaccine strain, and the efficient screening method comprises the following steps: (1) diluting a mother generation influenza virus to be screened by using a virus maintenance solution, adding the diluted mother generation influenza virus into a culture plate containing canine kidney cells, adsorbing the canine kidney cells by the influenza virus, and discarding a virus solution in the culture plate; (2) covering the surfaces of the cells with a maintenance medium in a liquid state, cooling and solidifying the maintenance medium, and inversely placing the maintenance medium in an incubator to culture viruses; and (3) selecting virus spots, mixing the selected virus spots with a buffer solution, inoculating the obtained mixture into chick embryos and / or canine kidney cells, and culturing to obtain the candidate vaccine strain of the influenza virus. The method provided by the invention is simple and easy to operate, short in time consumption, low in cost, few in passage times and not easy to mutate, and can achieve the purpose of quickly and effectively screening out influenza virus vaccine candidate strains with excellent growth characteristics from mother generation virus seeds in a short time.

The invention relates to an extraction and culture method of urine-derived stem cells, and belongs to the field of cell biology and regenerative medicine. The method comprises the following steps: coating a culture plate with a gelatin solution; collecting clean midstream urine of healthy people and adding antibiotics; centrifugally separating the cell precipitate; washing with a phosphate buffer solution; re-suspending the precipitate by using a urine stem cell complete culture medium for primary culture; and after the cells grow to a certain fusion degree, carrying out passage amplification. The urine stem cell complete medium comprises a basic medium, fetal calf serum, epidermal growth factors, platelet-derived growth factors and basic fibroblast growth factors. By adopting the method disclosed by the invention, the urine-derived stem cells can be efficiently and stably separated from urine, and the obtained cells have high multiplication capacity, good stemness maintaining capacity, multidirectional differentiation potential and immunoregulation function, and can provide an ideal seed cell source for treating diseases such as diabetic erectile dysfunction.

The utility model discloses a dust removal device for cell culture plate production, which relates to the field of automatic production and comprises a belt conveyor, a dust blowing mechanism is arranged on the upper side of the belt conveyor and used for impacting dust on a cell culture plate through airflow, and a dust collection component is arranged on the upper side of the belt conveyor; the dust blowing mechanism comprises a mounting frame and a moving assembly used for driving the mounting frame to reciprocate in the length direction of the belt conveyor. A plurality of air pipes are rotationally arranged on the mounting frame, and a plurality of air outlet holes are formed in the sides, facing the upper surface of the belt conveyor, of the air pipes. The device has the beneficial effects that the cell culture plate is moved to the bottom of the dust blowing mechanism through the belt conveyor, high-speed airflow is output to the cell culture plate through the air pipe, dust attached to the cell culture plate is blown up through the airflow, and dust around the cell culture plate can be absorbed through the dust absorption assembly; the dust blowing mechanism is matched with the dust collection assembly, so that dust on the surface of the cell culture plate and in holes can be thoroughly removed.

A tissue culture plate material retainer system includes a culture material retainer having a retainer ring with a retainer ring gap to enable the retainer ring to be compressed for insertion into a culture plate well and a plurality of retainer legs that extend down from the retainer ring to hold culture material down in the well of the culture plate in or under a culture medium. A culture material retainer has a retainer ring that extends along a top and a plurality of retainer legs that extend down from the retainer ring. The retainer ring extends from a first end to a second end forming a retainer ring gap that enables the retainer ring and culture material retainer to collapse or flex radially inward for insertion into a wall.

The utility model discloses a miniature cell culture chamber suitable for ultrahigh-flux living cell imaging. The miniature cell culture chamber comprises a culture box with an opening at the front end, a culture plate is connected in the culture box in a sliding manner; a plurality of imaging grooves are formed in the culture plate; an adapter for placing a culture vessel is arranged at the top of the culture plate; the adapters and the imaging grooves are the same in number and are arranged in a one-to-one correspondence mode. A CO2 inlet and an N2 inlet are formed in the top of the incubator; an air filter, a heating module and a humidifier are arranged in the incubator; a lamp panel is arranged at the top of the incubator; lED lamps are distributed at the bottom of the lamp panel in an array mode. A transparent imaging plate is arranged at the bottom of the incubator. Through cooperation of the culture plate, the imaging groove, the imaging plate, the adapter, the LED lamp and other components, the living cell ultra-high-flux imaging device can achieve living cell ultra-high-flux imaging, is convenient to operate, high in space utilization rate and small in internal space, and can guarantee uniformity of internal gas, temperature and humidity.

The invention provides a cell culture plate, and belongs to the technical field of biological experiment equipment. The multi-channel pipettor solves the problem that in the prior art, sample adding or sampling of a multi-channel pipettor with smaller sample spacing cannot be realized on a cell culture perforated plate with a larger single-hole area. The cell culture plate comprises a plate body provided with a plurality of culture holes distributed in rows and columns, the plurality of culture holes are divided into a plurality of groups of culture holes in sequence by taking two columns as one group, and the positions of two adjacent and parallel culture holes in each group of culture holes are distributed in a staggered manner. The multi-channel pipettor has the advantage that sample adding or sampling of a multi-channel pipettor such as an 8-channel pipettor or a 12-channel pipettor with smaller sample spacing can be realized on a cell culture perforated plate with a larger single-hole area.

The utility model relates to the technical field of life science, and provides a photographing and image analyzing system for a culture plate, which comprises a support device, an image acquisition device, a moving device and a control device, and is characterized in that the support device is used for placing the culture plate; the moving device is arranged above the supporting device; the image acquisition device is connected with the moving device and is suitable for shooting the culture plate hole by hole under the driving of the moving device so as to acquire an image of a sample in the culture plate; the control device is electrically connected with the image acquisition device and the moving device, the control device is used for controlling the moving device to move so as to drive the image acquisition device to shoot holes one by one, and the control device is further used for analyzing and processing images acquired by the image acquisition device. The photographing and image analyzing system for the culture plate can meet the high-throughput experiment requirement and cannot generate a parallax effect.

The present application relates to the field of in vitro cell culture technology, and specifically relates to a moat-type microwell culture plate. The plate comprises a microwell culture plate main body; a water-fillable trough is arranged on the microplate main body; microwells are arranged within the water-fillable trough, where the openings of the wells face upward; the water level in the water-fillable trough is lower than the openings of the culture wells, the water in the water-fillable trough surrounds the wells and forms a structure similar to a moat, and water vapor is produced when the water evaporates, thereby achieving a humidifying effect. This means of humidification is spontaneous and does not introduce bacteria, thus ensuring that the wells are not contaminated during incubation.

The utility model provides an air purification device. The air purification device comprises a fixing base, a rotating motor, a culture disc and a top cover. The fixing base is provided with a ventilation cavity, and a plurality of through holes are formed in the peripheral wall of the fixing base. The rotating motor is fixedly connected to the bottom surface of the ventilation cavity, and the power output end surface is provided with a slot; the upper surface of the culture tray is provided with a biological filtering material, and the lower surface is connected with a positioning rod suitable for being inserted into the slot; the top cover is detachably connected to the fixing base and provided with a light-transmitting opening suitable for facing the biological filtering material. During use, the fixed seat is fixedly mounted in an air circulation area, and external air enters the ventilation cavity through the through holes and then is purified by the biological filtering material. And when the biological filtering material needs to be replaced, the top cover is detached, the culture tray is taken out upwards, and the positioning rod is separated from the slot. The process of removing the biological filtering material is simple, and compared with the prior art, the air purification device provided by the utility model has the advantage that the regular maintenance efficiency of the biological filtering material is guaranteed.

The utility model discloses an edible fungus incubator networking alarm device, which relates to the technical field of edible fungus cultivation, comprises a box body, and is characterized in that a box door is arranged on one side of the box body, a sealing gasket is arranged on one side, close to the box body, of the box door, and three mounting blocks are arranged on each of two sides of the inner wall of the box body. And draining plates are arranged among the three groups of mounting blocks. The sealing effect between the box body and the box door can be improved through the arranged sealing gasket, external dust and impurities are prevented from entering the box body to influence thallus embryos, when the box body needs to be atomized and humidified, some small water drops in the culture groove are gathered together, water can fall into the draining groove through the draining holes, and therefore the thallus embryos are prevented from being damaged. Therefore, some water can be prevented from being deposited in the culture tank for a long time to influence thalli in the culture tank, the quality of produced edible mushrooms is ensured, the culture plate can be taken out by pulling the handle, the water collected in the draining tank can be conveniently recycled, and waste of water resources is avoided.

1. The name of the design product: micro-well array tumor organoid culture plate. 2. The use of the design product: for tumor organoid culture, tumor drug screening and personalized treatment. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: combination state diagram. 5. The right view of component 2 is the same as the left view of component 2, and the right view of component 2 is omitted; the bottom view of component 2 is the same as the top view of component 2, and the bottom view of component 2 is omitted. 6. Other circumstances that need to be explained Component explanation: component 1 is a well plate, and component 2 is a micro-well array chip.

The invention discloses a tooth root organ, a construction method and application thereof and an induction medium, and belongs to the technical field of biomedicine. The construction method disclosed by the invention comprises the following steps: reviving and resuspending engineered dental epithelial cells to prepare a dental epithelial cell suspension; dental sac stem cells are taken, re-suspended and then inoculated into a cell culture plate, the culture plate is centrifuged, the dental sac stem cells are gathered at the bottoms of culture holes to form dental sac stem cell clusters, a culture medium is added, culture is conducted, and dental sac stem cell microspheres are formed; and centrifuging the culture plate, absorbing the supernatant, adding a dental epithelial cell suspension, standing, and adding a culture medium for continuous culture, so that the dental epithelial cells are compounded on the surfaces of the dental capsule stem cell microspheres to form tooth root organs. The invention discloses the tooth root organoid obtained by adopting the construction method and application of the tooth root organoid. The invention further discloses an induction culture medium for inducing and differentiating the tooth root organoid.

The present invention relates to an automated machine for processing culture plates, comprising an operating unit for receiving culture plates in continuous succession and performing a series of successive treatments on each culture plate. The operating unit comprises a plurality of stationary processing stations (PT1-PT8) distributed at a predetermined interval around an axis (X2) and capable of being activated at least partially simultaneously. It also comprises a rotational drive device (DER) for the culture plates relative to the processing stations, having housings distributed according to the interval of the processing stations such that a rotation of the drive device around the axis brings a given culture plate successively to each of the processing stations. Figure 1

The invention discloses a culture method of in-vitro uniform monolayer cells of prawns, and belongs to the technical field of biology. The method comprises the following steps: (1) obtaining lymphatic tissues of a last-stage prawn at the early stage of shelling, completely stripping an outer membrane to obtain internal tissues, and cutting the internal tissues into small tissue blocks; (2) cleaning the small tissue blocks, inoculating the small tissue blocks to a culture plate coated with a gelatin solution, adding a complete culture solution into an inoculation system at intervals within 12 hours after inoculation for culture, replacing the complete culture solution 24-48 hours after inoculation, and inducing tissue cells to continuously migrate to form an in-vitro cell layer; (3) blowing and beating the cell culture to obtain a cell suspension, and centrifuging to obtain a cell monolayer; and (4) suspending the cell monolayer by adopting a complete culture solution, then inoculating the cell monolayer into a culture plate coated with a gelatin solution, and culturing to obtain the in-vitro uniform monolayer cells of the prawns. According to the method, the prawn monolayer cells which are long in in-vitro survival time, uniform and stable and are cultured in vitro can be obtained, and an important tool is provided for related virology research.

The utility model discloses a tumor cell scratching device, which relates to the technical field of cell experiments and comprises a worktable body, a protective baffle is fixedly mounted at the center of the upper surface of the worktable body, and a scratching component is arranged above the protective baffle. The motor is started, the output end of the motor drives the first gear to rotate, the first gear drives the double-face circular gear ring meshed with the first gear to rotate, and therefore the four second gears meshed with the inner side teeth of the double-face circular gear ring are driven to rotate; when each group of second gears rotate, a correspondingly meshed rack is driven to move in the worktable main body, and when the rack moves, a fixed rod fixed at the top of the rack is driven to synchronously move towards the direction of the culture plate, so that a telescopic rod and an L-shaped positioning block are driven to move towards the direction of the culture plate, and on one hand, the culture plate is quickly centered and accurately positioned; on the other hand, the culture plates with different sizes can be positioned, and the practicability of the device is improved.

The utility model relates to a cell co-culture experimental device easy to change liquid, which comprises a culture plate, and a liquid inlet device is arranged on the side wall of the culture plate close to the bottom so as to discharge new liquid into the culture plate; a liquid discharging device is arranged at the bottom of the culture plate and is used for discharging old liquid in the culture plate. The liquid inlet device comprises a liquid inlet pipe arranged on the side wall of the culture plate, the liquid inlet pipe is obliquely arranged, the liquid inlet direction inclines to the bottom of the culture plate, and an arc-shaped protrusion opposite to a liquid inlet outlet of the liquid inlet pipe is arranged on a bottom plate of the culture plate. The liquid discharging device comprises liquid discharging pipes arranged at the bottom of the culture plate, and the multiple liquid discharging pipes intersect and communicate with a liquid collecting pipe arranged at the bottom of the culture plate. According to the cell co-culture device, cell pollution can be avoided on the basis of quickly realizing cell liquid change, and meanwhile, the cell co-culture process is simplified.