36 results about "Culture plates" patented technology

The utility model discloses a kind of water culture devices for yunnan rhizoma polygonati breeding, it is related to yunnan rhizoma polygonati cultivation technical field, including culture rack, the culture rack is slidably connected with multiple water storage channels, and the water storage channel upper end is provided with culture plate;Multiple planting holes are evenly provided in the culture plate, and the culture plate upper end is connected with multiple fixed components, and fixed component is located planting hole outer end.The utility model design structure is reasonable, it can simultaneously carry different batches, different genotypes yunnan rhizoma polygonati breeding material, while independent water supply head, gate plate can individually adjust the water supply of each water storage channel, water supply frequency, realize the accurate management of "one tank one strategy", satisfy the individualized demand of different breeding material, to improve the success rate of breeding, can avoid the problem of "excessive supply" or "insufficient supply" caused by unified water supply, accurately match the demand of each growth stage of yunnan rhizoma polygonati, save water resource, nutrient solution and other consumables, reduce breeding cost.

The application discloses a construction method and application of an osteoarthritis cartilage organoid, and the method comprises the following steps: 1) mesenchymal stem cells are inoculated on a round bottom culture plate by a Micromass method, and are cultured in a chondrogenic induction culture medium containing dexamethasone, ascorbic acid and TGF-beta 3 for 10-18 days to form a transparent cartilage organoid; and 2) the transparent cartilage organoid is placed in an osteoarthritis induction culture medium containing dexamethasone, L-thyroxine, inflammatory factors (IL-1beta, IL-6 and TNF-alpha) and beta-glycerophosphate sodium for continuous culture for 5-10 days to obtain an osteoarthritis cartilage organoid; the model simultaneously presents multi-dimensional pathological characteristics such as matrix degeneration, hypertrophy, inflammation activation and vascularization trend, and can be used for osteoarthritis pathogenesis research, drug screening and cell treatment effect evaluation; the construction method is controllable and has good repeatability, and provides a reliable three-dimensional in-vitro model for osteoarthritis research.

The utility model discloses a cell ball culture pore plate which comprises a culture plate, a plurality of culture pores are arranged on the culture plate at intervals, and a plurality of pits are arranged in each culture pore. The cell ball culture device is easy to operate, a large number of uniform cell balls can be obtained in the same culture hole at a time when the cell balls are cultured, the balling efficiency and the balling number of the cell balls can be effectively improved, and the uniformity of experiments is ensured; according to the utility model, the specially designed buffer table can effectively prevent the cell ball from being flushed out of the pit due to the impact of liquid on the bottom of the pit during sample adding, and can prevent the cell ball from being sucked or injured by mistake due to the fact that a gun head is in contact with the bottom of the pit during pipetting; the characteristics of a traditional pore plate are reserved, flux is large, the appearance is matched with most detection machine types on the market, observation is convenient, and the device is suitable for various imaging technologies.

This invention discloses a fully automated cell culture incubator, comprising an incubator, a conveying mechanism, and a storage rack assembly. The storage rack assembly can store multiple sets of culture plates and is rotatably docked with the conveying mechanism. The conveying mechanism can grasp the culture plates on the storage rack assembly and convey them to the outside of the incubator for docking. The advantages of this invention are that the conveying mechanism can grasp and convey the culture plates on the storage rack assembly to the incubator, and simultaneously convey and dock external culture plates with the storage rack assembly. This device can store and culture batches of organisms through the storage rack assembly, seal the incubator through a sealing door assembly, and filter impurities inside the incubator to ensure a suitable growth environment for the samples.

The application relates to the technical field of seedling cultivation, and discloses a cutting device and a rapid propagation method based on a two-stage method of eagle tea, which comprises a top-opened water culture box, the top of the water culture box is detachably connected with a water culture plate, a plurality of through holes are arranged on the water culture plate, clamping assemblies are arranged in the through holes, the clamping assembly comprises wall grooves arranged on the two sides of the through hole and clamping parts located in the wall grooves; the clamping part comprises a clamping plate and an elastic sheet, the clamping plate and the elastic sheet can move transversely in the wall grooves and the through holes, the elastic sheet is arc-shaped, the elastic sheet is fixedly connected with the bottom of the clamping plate, and the interval between the free ends of the two elastic sheets in the clamping assembly is smaller than the interval between the two clamping plates; and the adjusting assembly for adjusting the interval between the two clamping plates in each clamping assembly is further arranged. The application mainly solves the problem that the existing fixing mode of tea seedlings is complicated to operate.

The utility model discloses a kind of epilithic microalgae fan-shaped culture devices, belong to microalgae aquaculture technical field, including culture box, the inner bottom middle position of culture box is provided with several epilithic microalgae culture plates in fan-shaped array distribution, the equidistantly of the side wall of culture box is provided with several culture light source.The utility model epilithic microalgae fan-shaped culture device effectively solves the technical bottleneck such as low culture efficiency, high cost of recovery, scale amplification difficult in the industrialization process of epilithic microalgae.

This invention discloses a co-culture immune isolation device after cell transplantation, belonging to the technical field of cell culture devices. The device includes a culture plate with several first cavities inside and placement slots outside the first cavities. A cover plate is provided above the culture plate, and several second cavities are provided on the cover plate. A sealing ring is provided on the outside of the second cavities, and a liquid inlet mechanism is provided inside the cover plate, connected to the second cavities. This invention uses the above-mentioned co-culture immune isolation device after cell transplantation to achieve co-culture of multiple cells by setting multiple independent first cavities, preventing cell migration or direct contact and avoiding immune rejection. The protective cover and liquid inlet mechanism achieve closed-loop automatic liquid inlet, continuously diffusing signal molecules into the first cavities to maintain co-culture homeostasis and achieve co-culture of multiple cells.

The present application provides a method for in vitro purified corneal limbal stem cells to be transdifferentiated into lens spherules, specifically, the present application inoculates corneal limbal stem cells onto a culture plate containing a Matrigel glue layer, and cultures the corneal limbal stem cells in a culture system containing Y-27632 additives in LSCM culture solution, so that lens spherule organoids can be obtained. The method of the present application can realize the rapid transdifferentiation of corneal limbal stem cells into lens spherules, so that they can be implanted into the capsular bag as seed cells for in situ regeneration of the lens.

This utility model discloses a chain-driven, segmented, movable, and environmentally adaptable mushroom rack, comprising: a main body, the main body including at least three segmented modules, adjacent segmented modules being connected by detachable connectors, each segmented module having multiple vertically arranged culture plates inside, and the surface of the culture plates having a fungal culture trough; a support frame, two support frames, with a chain drive mechanism between the two support frames for driving the segmented modules to move; the chain drive mechanism includes a vertically downward mounting plate located in the center of the support frame, with an active sprocket on the mounting plate; the main body of this utility model includes at least three segmented modules, and through segmented modular design, the spacing between the segmented modules can be adjusted according to the growth cycle, improving space utilization; and through the provided chain drive mechanism, multiple segmented modules can be moved synchronously, facilitating adjustment.

The utility model discloses a cell culture well plate that reduces impact force relates to cell culture technical field, including cell culture plate, be provided with injection liquid structure on the cell culture plate, and injection liquid structure includes multiple culture wells and injection liquid hole, and culture well and injection liquid hole all are set up on cell culture plate, and culture well and injection liquid hole are through, and the top of cell culture plate is provided with sealing cover, and the diameter of injection liquid hole is one fifth of culture well diameter, and injection liquid hole and culture well hole neck place are through, and the corner of sealing cover and cell culture plate is provided with mark groove, and a plurality of sealing grooves are seted up on sealing cover and are fixedly connected with lug, and the edge of sealing cover is provided with sealing washer, through being provided with injection liquid structure, the injection liquid hole in injection liquid structure can avoid culture liquid to directly drop in culture well, avoid the cell in culture well inside to cause impact force and vibration, avoid the normal growth of cell.

This invention belongs to the field of biology and discloses a method for co-culturing enteric glial cells (EGCs) and intestinal organoids. Primary cultured and purified enteric glial cells are digested and seeded onto the membrane of a Transwell chamber. Intestinal organoids are placed in individual Matrigel drops in a co-culture plate. The Transwell chambers are placed in the co-culture plate, and growth medium is added to ensure free exchange of the growth medium through the Transwell membrane. The EGC-organoid co-culture system obtained based on this method provides a model for studying diarrhea and intestinal health in large mammals. It also lays the research foundation for developing innovative intervention strategies targeting the enteric nervous system and provides a research model for further exploring the regulatory roles of nutrients or related genes in intestinal health.

The present disclosure describes automated systems and methods for handling objects, such as culture plates or dishes. For example, in one embodiment, the present disclosure describes an automated stacker and de-stacker comprising a clamping mechanism, a lift pad, a pair of pins, and a cabinet. A stack of culture plates may be stored in the cabinet. During a stacking operation, the pair of pins may be raised to stop a culture plate traveling along a conveyor track. Once stopped, the culture plate may be raised above the conveyor track by the lift pad and clamped by the clamping mechanism. During a de-stacking operation, the clamping mechanism may be opened, and the culture plate may be lowered onto the conveyor track by the lift pad.

This invention discloses an illumination device for solving the concave liquid surface effect in well plate imaging, belonging to the field of microscope light source technology. The device includes an inverted microscope illumination source and several annular light sources distributed in a dome-like pattern around it. The inverted microscope illumination source is composed of a central light source, a collimating lens, and a condenser lens in sequence. The annular light sources contain multiple LED beads, and the size of the rings matches the diameter of the microwells in a common cell culture plate, with adjustable angles. Through the synergistic effect of traditional vertical illumination and dome-shaped oblique illumination, this invention effectively improves the imaging quality of well plates, adapts to microwell plates of different diameters, is easy to operate, and highly practical, providing strong support for cell observation and other related work.

This invention discloses a xenogeneic-free culture method for hiPSCs and its quality monitoring method. The xenogeneic-free culture method includes the following steps: hiPSC cells are seeded into the wells of a culture plate coated with VTN-N matrix gel, and cultured for the first stage using E8 complete medium containing 5-15 µM Y-27632. After 20-30 h of culture, the medium is replaced with conventional E8 complete medium without Y-27632 for the second stage of culture, until the cell confluence reaches 60%-80%. This xenogeneic-free culture method ensures efficient cell expansion under conditions with clearly defined chemical composition. This method further optimizes the seeding density and culture period of hiPSCs, controls cell passage at 60%-80% confluence, and significantly reduces the risk of spontaneous differentiation by limiting the maximum culture time and upper limit of confluence.

The utility model discloses a kind of co-culture plate of neuron and glial cell, it is related to cell co-culture technical field, including culture plate and climbing sheet, the support frame of open shape is arranged in culture plate, the middle position of support frame is provided with multiple climbing pipes of annular distribution, vertical arrangement, the top of climbing pipe is all inclined to the center position of support frame, the bottom of climbing pipe is contacted with culture plate;The center position of climbing sheet is provided with insertion hole, the insertion hole is sleeved in the outside of climbing pipe, and insertion hole inner wall is contacted with the outer wall of climbing pipe;The lower surface of climbing sheet is supported on support frame.The utility model designs the support frame with climbing pipe and the climbing sheet with insertion port, climbing sheet can be inserted on support frame, with the advantage of more convenient installation and use.

The invention discloses a microbial colony counting and morphology intelligent analysis method and system, and relates to the technical field of image processing and microbial detection.The method comprises the steps that a multi-modal imaging module collects images of a culture plate in a transmission light and annular lateral light dual mode and fuses the images to generate a multi-modal image; the bacterial colony instance segmentation module performs pixel-level instance segmentation on the fused image by adopting a multi-scale feature fusion network in combination with a channel-space double attention mechanism, and outputs boundary masks of all bacterial colonies; the morphological feature extraction module calculates geometric, texture and optical parameters to construct morphological feature vectors; the strain primary screening module outputs a candidate strain list and confidence through weighted Euclidean distance matching, the problems that manual counting efficiency is low, adhesion bacterial colonies are missed in weighing, and culture medium background interference exists are solved, and the counting accuracy rate reaches 98% or above.

This invention discloses a model data standardization processing method and system for drug testing, relating to the field of biomedical technology. The method involves collecting cell culture plate numbers, drug concentrations, culture times, and absorbance readings; sorting concentrations and matching absorbance to form experimental sequence values; filtering for consistency in well readings within the same plate to eliminate deviations; normalizing absorbance ratios and generating response coefficients through difference; correcting for deviations in the mean values ​​of different batches to form correction coefficients; and superimposing the correction coefficients and mapping them to the concentrations to rearrange and output standardized drug efficacy data. This invention's model data standardization processing method and system for drug testing synchronizes the cell culture plate numbers and time scales in the original experimental records and performs gradient sorting of drug concentrations, enabling data under different time and dosage conditions to form a sequentially constrained sequence structure, enhancing the correspondence between experimental data in the time and dosage dimensions.

The utility model relates to a kind of porous cell culture plate, including culture plate component, culture plate component includes culture plate, there are several groups of PCR manifold in the bottom of culture plate along left and right direction evenly, and the first connecting part and the second connecting part are detachably mounted together between the two groups of PCR manifold adjacent to left and right, each group of PCR manifold includes several PCR tubes distributed along front and back direction;Support component is provided below culture plate.The utility model is placed in the support component below culture plate component, ensure that culture plate is in horizontal state after being placed, prevent culture plate from shaking left and right, can provide stable support and accurate positioning function.

This utility model relates to the field of cell culture technology and discloses a cell culture plate transfer device, including a base, a top cover on the top of the base, a cavity between the base and the top cover for placing the cell culture plate, a retaining plate fixedly connected to the side wall of the base, a fixing frame fixedly connected to the side wall of the top cover, a snap plate rotatably connected to the side wall of the fixing frame, a cavity inside the snap plate, the end of the retaining plate fitting into the cavity, and a spring between the top cover and the snap plate. In this device, the sliding contact structure between the top cover and the base, the cooperation between the retaining plate and the snap plate, and the automatic return function of the spring ensure the stability of the connection between the base and the top cover. Placing the cell culture plate in the cavity between the base and the top cover effectively prevents cell contamination caused by the cover slipping or accidentally opening during the transfer process. Furthermore, this transfer device is easy to open and close quickly, improving the convenience and stability of operation.

The application belongs to the technical field of cell culture and specifically relates to a cell culture plate for organoid culture and a preparation method thereof, which is formed by physical adhesion of a coating solution to the surface of the cell culture plate and solidification. The coating solution comprises: polymer A, which is a hydrophilic material with a hydrophilic-lipophilic balance value greater than 16; polymer B, which is a polyether substance; and polymer C, which is a non-ionic surfactant; wherein the weight ratio of the three is 1-10:0.1-0.2:0.1-0.5; the cell culture plate is a polystyrene culture plate; and the water contact angle of the coating is 10-60°. Compared with the prior art, the application solves the problem that the coating can be attached only by chemical cross-linking, surface activation or surface grafting in the prior art. The ultra-low adsorption cell culture plate has the following advantages: ultra-low cell adhesion; simple preparation process; no chemical cross-linking agent residue; stable physical and chemical properties; and compatibility with the existing standard production process of the culture plate material.

The utility model relates to the technical field of culture plate stacking, and disclose a kind of gripper structure of three-warehouse cooperative stacking device, comprising: the gripper motor of installation on the stacking warehouse, two output ends of the gripper motor are respectively equipped with first connecting arm and second connecting arm, and the opposite surface of first connecting arm and second connecting arm is equipped with connecting piece;The top of two connecting pieces is equipped with gripper head, and the bottom of two connecting pieces is equipped with guide component;The gripper head includes the connecting cross bar for installation on connecting piece.This gripper structure of three-warehouse cooperative stacking device, by setting connecting cross bar, clamping part and backing plate part, standard microorganism culture plate enters stacking warehouse, starts gripper motor, gripper motor drives first connecting arm and second connecting arm to move towards each other, drives gripper head to clamp, clamping part clamps the side of standard microorganism culture plate, while backing plate part holds standard microorganism culture plate, to avoid standard microorganism culture plate to drop.

This invention discloses a tissue culture chamber, comprising an inner chamber, an outer ring, and an outer ring connecting portion. The inner chamber includes an inner chamber wall and an inner bottom, with the inner bottom disposed within the cavity formed by the inner chamber wall. A central hole is provided on the inner bottom, and inner bottom leakage holes are distributed on the inner bottom around the central hole. The inner chamber wall includes a top ring and inner chamber pillars circumferentially distributed at the bottom of the top ring. The outer ring is disposed on the outer side of the inner chamber, and the outer ring is connected to each of the inner chamber pillars through the outer ring connecting portion. The tissue culture chamber of this invention can stably support cultured tissues on cell culture plates or cell culture dishes, is convenient and quick to use, and can be reused multiple times to reduce costs.

The utility model discloses a detachable culture plate, relates to culture plate technical field, and its technical key points include dismounting and assembling seat, culture plate board body, culture plate board body installation connection in the upper end inside of dismounting and assembling seat, culture plate cover, culture plate cover sleeve joint in the upper end circumference side of dismounting and assembling seat, upper rotary assembling plugboard, upper rotary assembling plugboard rotation installation is in the right -hand side upper side of dismounting and assembling seat, lower rotary assembling plugboard, lower rotary assembling plugboard rotation installation is in the left -hand side lower side of dismounting and assembling seat, and technical effect is that dismounting and assembling seat can be assembled left and right through lower rotary assembling plugboard and upper rotary assembling plugboard, so this can effectively combine together and use, and the lower rotary assembling plugboard is installed to the lower assembling slot of base shell through corresponding insertion, and the upper rotary assembling plugboard is installed to the upper assembling slot of base shell through corresponding insertion, thereby can be assembled in one -to -one correspondence.

The application relates to the technical field of plant tissue culture, and particularly discloses a fig tissue culture device and a culture method, which comprises a transparent cover body, a container and a culture plate. The container is internally provided with a plurality of containing grooves, the orientations of all containing groove openings are consistent, one side of the culture plate is provided with convex blocks, the number of which is not less than that of the containing grooves, the other side of the culture plate is provided with culture grooves corresponding to the positions of each convex block, and the bottom of each culture groove is provided with a plurality of through holes. The fig tissue culture device avoids cross-area transmission of bacterial contamination, improves the safety of the fig tissue culture device and the success rate of explant tissue culture, the fig tissue culture method realizes batch replacement of culture liquid, avoids bacterial contamination of the explant from external air, and improves the survival rate of long-period culture of the explant.

The utility model provides a kind of cell culture plate, belong to biological experimental equipment technical field.It solves the problem that the smaller sample spacing multichannel pipettor sampling or sampling cannot be realized on the cell culture multi-well plate with larger single-hole area in prior art.This cell culture plate includes a plurality of row-column distribution culture holes plate body, a plurality of culture holes are divided into multiple groups of culture holes with two columns as a group in sequence, the position of adjacent and parallel two culture holes in each group of culture holes is staggered distribution.The utility model has the advantage that smaller sample spacing multichannel pipettor such as 8 channels or 12 channels pipettor sampling or sampling can be realized on the cell culture multi-well plate with larger single-hole area.

The utility model relates to biological experiment equipment technical field, concretely relates to a cell culture plate, the cell culture plate includes: culture plate main part, culture hole, set up in the upper surface of culture plate main part, is enclosed by bottom wall and side wall, side wall sets up in the side of culture hole, and is connected with culture plate main part, bottom wall is connected in the below of side wall, and bottom wall is the taper type of wide on the top narrow, mounting groove sets up in the inside of side wall, limit along, through mounting groove, makes detachable connection between limit along with side wall. The utility model discloses through setting limit along, increase the limiting resistance of cell liquid in culture hole in the process of oscillation or shaking, limit the fluctuation amplitude of cell liquid in culture hole in the process of oscillation or shaking, under the condition of guaranteeing the cell liquid cell concentration uniformity, avoid cell liquid to splash out culture hole, effectively guarantee the accuracy of tumor cell proliferation inhibition experiment.

This invention relates to the field of microbial detection technology and discloses a method for detecting the colony content of cosmetics based on an intelligent sensing system. The method includes: projecting three-phase phase-shifted structured light onto a culture plate, demodulating to obtain the diffuse reflection alternating component matrix, and calculating the reduced scattering coefficient matrix to decouple static scattering interference; subsequently extracting the true microbial absorption coefficient matrix excluding powder interference, and calculating the speckle time contrast matrix in conjunction with continuously acquired speckle time series; exponentially fusing the two to construct a biochemical and physical activity fusion index matrix, and obtaining the cumulative biomass through local background smoothing and spatial integration; finally, adaptively generating a judgment threshold based on the global data of the culture plate, performing step-constraint screening to remove artifacts, and calculating the absolute total colony count. This invention effectively overcomes the problem of serious early false detections caused by cosmetic matrix artifacts, achieving accurate, closed-loop quantitative identification of live colonies without dependence on external sample libraries.

The utility model discloses a kind of cell amplification multi-well plates of gas-liquid exchange, belong to cell culture technical field, including culture plate body and cover plate, the culture plate body top is equipped with multiple large holes, culture well is arranged in the large hole, the bottom of large hole and culture well is connected, the culture well bottom is provided with gas permeable membrane by annular groove, the culture well bottom is equipped with connecting hole, the large hole outside edge is equipped with isolated boss, the culture plate body bottom is provided with gas exchange assembly, the utility model is when using, the rounded table shape of culture well increases gas-liquid contact area, combine the good gas permeability of gas permeable membrane, and design independent air inlet pipeline and air outlet pipeline and each culture well are communicated, can realize efficient gas-liquid exchange, provide sufficient oxygen for cell, timely discharge carbon dioxide, ensure that cell grows under good gas environment, promote the proliferation and activity of cell keep.