799 results about "Culture plates" patented technology

A cell culture plate that includes an upper portion having at least one discrete chamber with a top edge and a bottom edge; a substantially flat lower portion, wherein at least one layer of polymer fibers has been deposited on the upper surface of the lower portion, and wherein the at least one layer of polymer fibers is conducive to the growth of biological cells thereon; and wherein the bottom edge of the at least one discrete chamber is hermetically sealed to the fiber-coated upper surface of the lower portion to form a well using adhesives, laser welding, or ultrasonic welding.

The present invention relates to a three dimensional construct formed from non-biodegradable and non-cytotoxic polymers that provide an internal and external space for living cells to attach, proliferate and differentiate. The construct is composed of polymer struts and/or fibers which are joined together in a designed 3 dimensional pattern. The 3 dimensional cell culture construct (cell culture insert) is intended to be used together with cell/tissue culture plate, tissue culture flask, bioreactor and the like under normal cell culture conditions. The invention further provides methods of making the 3 dimensional cell culture construct. Finally, the invention provides kits comprising one or more 3 dimensional porous cell culture construct in a package together with other cell culture supplies, such as tissue culture plate and flasks.

The invention relates to an automatic bacteria culture instrument, which comprises a sealed incubator. A rotatable sample disc is arranged in the middle of the incubator; at least one culture bottle base is arranged at the peripheral edge of the rotatable sample disc; a biphase culture bottle is arranged inside the culture bottle base; a mobile device is arranged above the rotatable sample disc; a mechanical hand capable of gripping the biphase culture bottle and enabling the biphase culture bottle to overturn up and down is arranged on the mobile device; and the mobile device is also provided with a camera device facilitating dynamic observation of a culture plate in the biphase culture bottle. The automatic bacteria culture instrument reduces complex manual culture operation programs, shortens the culture time, and is convenient to early discover positive samples. The infection risk due to the contact between the operating personnel and the samples is avoided, and the health of the operating personnel is guaranteed; and the positive results of the samples delivered to the lab can be timely discovered and reported, and precious time for the clinical service is obtained.

The invention relates to a method for artificial culture and propagation of plant root-knot nematodes and for species preservation. The propagation and preservation of the root-knot nematodes in a lab depend on host plants all the time; the invention places a relatively small amount of root-knot nematode ovums or 2nd instar larvas in a particular dual culture medium rather than host roots and performs a upside-down culture under conditions of constant temperature and hermetic closure, the root-knot nematodes ingest, grow and propagate inside the culture medium and a large amount of nematomorphas can be obtained in a short term; additionally, a culture plate is timely transferred to be located under cold conditions, and dish change and subculture are implemented once within a half year so that the propagation and preservation processes of the root-knot nematodes are carried out without the host plants. The inventive advantages lie in: saving the culture program of the root-knot nematodes to host the plants without losing the infectivity of the root-knot nematodes on sensitive host plants; the employed materials have low costs and the experiment method is easy in operation; the invention provides a simple, convenient and fast propagation method for the species preservation of the root-knot nematodes, in particular for a large number of nematomorphas as required in the related researches of the root-knot nematodes.

The invention belongs to the field of microalgae culture, and particularly relates to an inserting-plate type microalgae semi-dry solid adherent culture device. The inserting-plate type microalgae semi-dry solid adherent culture device is characterized by comprising a supporting truss, culture plates, liquid distributing pipes, liquid collecting tanks and a liquid storage bottle, wherein the supporting truss is provided with at least one culture plate, and one liquid distributing pipe is arranged above each culture plate; the liquid collecting tank arranged on the supporting truss is arranged under each culture plate; all the liquid collecting tanks are communicated with the liquid storage bottle by the main liquid pipe; the bottom of the liquid storage bottle is respectively provided with a circulating pump and a carbon-supplementing gas distributing pipe communicated with a carbon dioxide source, and the circulating pump is communicated with a liquid distributing pipe by a pipeline; and each culture plate is provided with a picking scraper. The inserting-plate type microalgae semi-dry solid adherent culture device has the advantages that the yield in unit occupied area is effectively increased, the culture period is shortened, the water consumption for culture is greatly reduced, the cost and the operating cost of the culture device are reduced and the industrial culture on large scale is easily realized.

The present invention provides a microbial sample processing device, comprising: a machine body provided with a machine chamber and a sealed movable door; and a sealed housing, a stretchable airbag, a sample tray, a mechanical arm, a bar code scanner, a liquefier, culture dishes, a gas-temperature sensor, and a temperature control device which are arranged in the machine chamber, wherein the sample tray is provided with a plurality of sample seats, the sample seats are used for placing the culture dishes, the liquefier is communicated with the culture dishes, each culture dish is provided with culture plates and a streak-inoculation pen used for streak inoculating the sample in the culture dishes from the liquefier on the culture plate. The microbial sample processing device is reasonable in structure, fast and safe, reliable and efficient, can complete the whole process of mixing and liquefying, streak inoculation, dilution and separation, and incubation of microbial community automatically in a one-stop manner, and can replace manual labor and ensure biological safety.

The present invention is concerned with an inlay (10) for a culture plate (20) for microorganisms or cells, a culture plate unit (30) for microorganisms or cells comprising such inlay (10), a culture system for microorganisms or cells comprising such culture plate unit (30), and a method for preparing a culture plate system (40) for microorganisms or cells with such inlay (10). Further, several uses as described herein are part of the present invention. The grid structure (11) is configured to fit into the culture plate (20) and is provided with a plurality of openings (12). The openings (12) are angled. In an embodiment, the openings (12) of the inlay (10) may be rectangular or square.

The present invention relates to a method for differentiating stem cells into vascular cells by culturing them in the form of a three-dimensional cell cluster and the use of the three-dimensional cell cluster for angiogenesis. Specifically, the present invention teaches a method for differentiating stem cells into vascular cells comprising culturing stem cells by adhering them onto a culture plate with a surface having a hydrophobic property or a culture plate onto which a growth factor is immobilized, wherein the cultured stem cells are later detached from the culture plate as their density increases to form a three-dimensional cell cluster and grown in the form of a three-dimensional cell cluster while differentiating into vascular cells. Also disclosed is the use of a three-dimensional cell cluster composed of the vascular cells differentiated from stem cells by the above method as a cell therapy agent for angiogenesis.

The invention discloses a culture solution for inducing mesenchymal stem cells to differentiate into islet-like cells, and an inducing method and application of the culture solution. The culture solution comprises the following materials: niacinamide, Conophylline, cell growth factor, betacellulin and a base medium. The base medium contains 97% of high glucose DMEM (Dulbecco Modified Eagle Medium), 2% of B-27 and 1% of N-2. The inducing method comprises the following steps of: preparing an inducing culture solution; preparing the human mesenchymal stem cells; taking the human mesenchymal stem cells, inoculating the human mesenchymal stem cells into a six-hole ultralow absorption culture plate by 1.5-2*105cells/hole, adding 3ml inducing culture medium into each hole and carrying out suspended induction; and changing liquid at every 3 days, collecting cell supernatant at the ninth day, and storing the cell supernatant at the temperature of -20 DEG C. The culture solution disclosed by the invention has the advantages that the human mesenchymal stem cells are induced to differentiate into the islet-like cells by utilizing the combination of the niacinamide and the Conophylline, so that the inducing cycle is shortened, the suspension cells are beneficial to being clustered to form cell clusters similar to natural islets, further the induced differentiation efficiency is obviously increased and the clinical application risk is reduced; and the function of inducing the secretion of the cell insulin is obviously improved.

The invention relates to an apparatus (1) for culturing anchorage-dependent cells. The apparatus (1) comprises a housing (2) with an inlet (4) and an outlet (5), and a plurality of culture plates (6) removably stacked within the housing (2). The housing (2) has a circumferential wall (7), a base (8) and a top wall (9). The base (8) comprises the inlet (4) and the top wall (9) comprises the outlet (5). The circumferential wall (7) of the housing (2) defines a longitudinal axis thereof, as well as an inner cross section perpendicular to the longitudinal axis. Shape and dimensions of the inner cross section are essentially uniform along the longitudinal axis. The culture plates (6) are arranged at least essentially parallel to each other. Each plate (6) is mounted and sealed to the circumferential wall (7) of the housing (2). Plates (6) are arranged at a distance from each other. Each culture plate (6) has a through hole (14), so that inlet (4) and outlet (5) are in fluid communication. Each through hole (14) is positioned at an outer end of the respective culture plate (6), proximate the circumferential wall (7). Through holes (14) of adjacent culture plates (6) are distally positioned in the plane of the inner cross section of the housing (2).

The invention relates to a device and a method for regulating and controlling the density of soilless cultured plants of a plant factory. The device comprises at least one culturing plate and at least two culturing units, wherein each culturing unit is provided with a culturing hole; each culturing plate is provided with a strip-shaped opening; the culturing units are erected on the strip-shaped openings and can move along the strip-shaped openings so as to regulate the interval; at least one additional culturing plate for regulating the interval between the culturing plates is arranged between the culturing plates; the culturing plates and the culturing units can be provided with matched guide rails for providing convenience for sliding. According to the device disclosed by the invention, each culturing unit moves along the strip-shaped opening of each culturing plate so as to regulate the planting distance; line spacing can be regulated by adding or reducing the additional culturing plates, so that the distance between the soilless cultured plants can be conveniently regulated; a culturing space can be saved; the utilization rate of electric energy of a light source can be increased.

In one aspect, the present invention relates to a mammalian cell-based high-throughput assay for the profiling and screening of human epithelial sodium channel (hENaC) cloned from a human kidney c-DNA library and is also expressed in other tissues including human taste tissue. The present invention further relates to amphibian oocyte-based medium-throughput electrophysiological assays for identifying human ENaC modulators, preferably ENaC enhancers. Compounds that modulate ENaC function in a cell-based ENaC assay are expected to affect salty taste in humans. The assays described herein have advantages over existing cellular expression systems. In the case of mammalian cells, such assays can be run in standard 96 or 384 well culture plates in high-throughput mode with enhanced assay results being achieved by the use of a compound that inhibits ENaC function, preferably an amiloride derivative such as Phenamil. In the case of the inventive oocyte electrophysiological assays (two-electrode voltage-clamp technique), these assays facilitate the identification of compounds which specifically modulate human ENaC. The assays of the invention provide a robust screen useful to detect compounds that facilitate (enhance) or inhibit hENaC function. Compounds that enhance or block human ENaC channel activity should thereby modulate salty taste in humans.

The invention belongs to the technical field of biological medicines and particularly relates to a detection method for anti-CD20 monoclonal antibody binding activities. The method comprises the following steps: a. taking a logarithmic growth phase high-expression CD20 cell, carrying out cell counting, and adjusting the cells to proper cell density for planking; b. carrying out series gradient dilution on a reference and a sample to be detected, and adding culture plates planked with the cells in sequence for a period of time; c. collecting the cells, and adding secondary antibody incubation marked with fluorescein isothiocyanate (FITC) for a period of time; and d. detecting by a flow cytometry to obtain the bonding activities of the sample to be detected. With the adoption of the detection method, the requirements in checking aspects of specificity, precision, linearity, range, accuracy and durability and the like can be satisfied; and the method can be effectively used for detecting the anti-CD20 monoclonal antibody binding activities.

The invention discloses a method for detecting the activity of abdominal cavity phagocytic cells by a flow cytometry, which aims at providing a method for fast, accurately, simply and easily detecting the activity of the phagocytic cells through the quantitative analysis on the number of phagocytic yeasts of the phagocytic cells on the basis of eliminating the influence of unendocytosed fluorescence yeast cells on the analysis results. The method comprises the following steps: preparing beer yeast cell freeze-dried powder; using FITC to mark the beer yeast cells; carrying out incubation on the suspension liquid of the fluorescence marked beer yeast cells and the blood serum of the mice at the room temperature; adding the suspension liquid of the abdominal cavity phagocytic cells into a hole of a culture plate; adding the fluorescence marked beer yeast cells after the incubation with the blood serum of the mice and the identical suspension liquid of the abdominal cavity phagocytic cells into the rest holes in the cell culture plate; carrying out wall adherence incubation on the cell culture plate; and using the flow cytometry to obtain data and carrying out analysis on the data. The method of the invention accurately measures the phagocytic rate and the phagocytic index, and has good repetitiveness.

The invention relates to a culture method for plant seedlings. The plant seedlings are cultured by a ventilated root division culture device, the side surfaces of two culture bowls of the device are in butt joint, a cover plate and a culture plate are arranged on the two culture bowls, culture holes are formed in the culture plate, and vent holes are formed in the two sides of the culture holes. The culture method comprises the followings steps: the plant seedlings of 3 days are taken and cultured in a Hoagland's nutrient solution with the concentration of 50 percent for 2 days then in a Hoagland's nutrient solution with the concentration of 100 percent for 12 days, the plant seedlings growing consistently are taken, the stems of the plant seedlings are wrapped by nutrient cotton and fixed in the culture holes of the culture plate, the roots of the plant seedlings are divided into a group A and a group B, the group A is put into the culture bowl containing the Hoagland's nutrient solution, and the group B is put into the culture bowl containing a nutritional deficiency nutrient solution; the pH values of the nutrient solutions are regulated to 6 at 8 o'clock in the morning and 15 o'clock in the afternoon; the nutrient solutions are changed every other three days; and after 4-12 days, the plant seedlings are taken out. The operation is simple, and the personal errors are reduced, so that the research result is more accurate, the culture time is saved, and the working efficiency is improved.

Embodiments of this invention include flatbed scanners that have a surface and have a plurality of light sources. Alternatively, a single light source can be moved in two dimensions relative to an object placed on the surface of the scanner. Other embodiments include a scan head that is moveable in two dimensions relative to the flat bed of the scanner. Further embodiments include one or more polarizing filters to reduce glare and increase the quality of a captured image. Theses scanners can be used to capture images of enzyme linked immunosorbent assay (ELISA) substrates, plates having cultures thereon or other objects having irregular lower surfaces. Additional embodiments include methods for capturing images from ELISA substrates, bacterial culture plates, viral plaque assay plates and the like.

The invention relates to a modified zebra fish drug metabolism modeling method, which specifically comprises the steps of (1) enriching metabolites by virtue of a zebra fish metabolism model; (2) separating and analyzing the enriched metabolic components of the zebra fish in the last step by using modern chromatography or the hyphenated technique thereof, capturing or knocking out target components or metabolites; (3) collecting zebra fish germ cells; (4) putting zebra fish embryos in three days after fertilization in a culture plate which holds an embryo culture medium and administrating a drug; (5) dyeing the bones of the zebra fish; and (6) detecting and analyzing the dyed bones of the zebra fish. According to the invention, an adolescent zebra fish osteoporosis model is applicable to in-vivo effective evaluation of anti-osteoporosis activity of Chinese herbal medicinal ingredients such as trace ingredients; an adult zebra fish metabolism model is simple in metabolites and efficient in enrichment; the modern chromatography or the hyphenated technique thereof provide powerful guarantee for the separation and analysis of the complex Chinese herbal medicinal system and the metabolic components. The model obtained by the method has the outstanding advantage that the separation and analysis of Chinese herbal medicinal original-form ingredients and the metabolic components are integrated with the in-vivo anti-osteoporosis activity evaluation, and simple and efficient.

The invention discloses a micropatterned hydrogel coating, its preparation method and use. The preparation method comprises the following steps: using one of carrageenan, locust bean glue, Arabic gum, xanthan gum, brown algae glue, brown algae sugar glue, xylan, agarose, mannan, galactomannans and glucomannans as a raw material to prepare a hydrogel solution first; spreading the hydrogel solution on a carrier such as a three-dimensional cell ball culture dish or a culture plate and the like; obtaining the coating with lattice patterns uniformly distributed on the surface through moulding and solidification demoulding. The micropatterned hydrogel coating may be used to produce culture products, and the culture products may enable a plurality of cells to propagate in a uniform multicellular ball way without other exogenous power so that the batch preparation of three-dimensional multicellular balls may be realized. The raw materials are easily available, quite low in cost, and natural and toxicity-free; the prepared coating belongs to a transparent nondiffusible coating; the growth of the cells in an animal body is highly simulated; and the coating has excellent stability and biological activity and is applicable to most cell culture products.

The invention provides a surface growth type culture plate with a membrane-coated porous plate structure. The culture plate comprises one layer of porous plate, wherein the porous plate is prepared from a rigid water absorption and water seepage material; the porous plate has a first pore diameter; each of the two sides of the porous plate is coated with one layer of ultra-micro-pore membrane; each ultra-micro-pore membrane has a second pore diameter; the second pore diameter is less than the first pore diameter; and the size of the second pore diameter of each ultra-micro-pore membrane can prevent bacteria or algae from entering and allows water and inorganic salt to seep. A culture unit comprises at least one culture plate, a solution supplying device and at least one culture solution recycling device, wherein the liquid supplying device is used for providing a culture solution to the culture plate; and the culture solution recycling device is arranged at the lower end of the culture plate. Furthermore, the invention relates to a surface growth type culture system containing the culture unit. According to the surface growth type culture plate with the membrane-coated porous plate structure, the problems of an existing immersion type microalgae culture photobioreactor system that the manufacturing and maintenance costs are expensive, the space utilization rate is low, the production efficiency is low and the energy consumption is high are solved.

The invention discloses a culture and preparation method for pyricularia oryza conidia. According to the method, the multi-starting-point culture technology and the aerial mycelium conidium production technology are utilized for fast culturing and preparing the pyricularia oryza conidia. The method comprises the following implementation steps that 1, a conidium production culture medium is prepared; 2, a multi-starting-point transplanting thallus is prepared; 3, the multi-starting-point transplanting thallus is planted in a culture plate; 4, the conidia are prepared and cultured; 5, the conidia are eluted and separated. The method has the advantages that fastness and high efficiency are achieved, and conidia of a new generation can reach the ideal state of a high sporulation quantity after five days of culture usually; sterile operation can be kept in the whole culture process, and the contamination probability of a finished conidium solution is greatly reduced; the operation technology is simple, and the result sporulation quantity is large.