314 results about "Culture plates" patented technology

The present invention refers to a method for coating a collagen like protein onto a surface of a polymer-scaffold comprising or consisting the following steps: a) plasma-treating the surface of the polymer-scaffold, b) providing a collagen like protein in a solvent, to obtain a collagen like protein-containing solution, c) bringing the plasma treated polymer-scaffold and the collagen like protein containing solution into contact followed by an optionally rinsing, d) removing the solvent and thereby obtaining a collagen like protein coated polymer-scaffold. Furthermore, the present invention refers to an article obtained by the method of the present invention. Moreover, the present invention refers to the use of the article according to the invention for wound healing and bone repair as well as for flask-coating and plate-coating in cell culture.

The present invention refers to a method for coating a collagen like protein onto a surface of a polymer-scaffold comprising or consisting the following steps: a) plasma-treating the surface of the polymer-scaffold, b) providing a collagen like protein in a solvent, to obtain a collagen like protein-containing solution, c) bringing the plasma treated polymer-scaffold and the collagen like protein containing solution into contact followed by an optionally rinsing, d) removing the solvent and thereby obtaining a collagen like protein coated polymer-scaffold. Furthermore, the present invention refers to an article obtained by the method of the present invention. Moreover, the present invention refers to the use of the article according to the invention for wound healing and bone repair as well as for flask-coating and plate-coating in cell culture.

The invention provides a method for producing cell culture meat through 3D scaffold-free co-culture of pseudosciaena crocea stem cell spheres, and belongs to the technical field of preparation of cell culture meat. A method for producing cell culture meat by 3D scaffold-free co-culture of pseudosciaena crocea stem cell spheres comprises the following steps: mixing and inoculating pseudosciaena crocea myoblasts and pseudosciaena crocea precursor adipocytes into a cell culture plate subjected to anti-adhesion treatment for proliferation culture, spontaneously gathering to form cell spheres, and carrying out differentiation culture to obtain the cell culture meat. According to the co-culture mode provided by the invention, high-efficiency myogenic differentiation and adipogenic differentiation of myoblasts and precursor adipocytes of the large yellow croakers can be realized through proliferation culture and differentiation culture, and an in-vivo microenvironment can be effectively simulated, so that cultured meat similar to a traditional meat product in texture and flavor is produced.

The invention discloses a high-flux organoid culture liquid core device which comprises an upper cover module, a transwell module and a porous culture plate, more than one culture hole is formed in the porous culture plate, a liquid inlet hole and a liquid outlet hole are formed in the bottom of each culture hole, at least one culture liquid circulation channel is formed in the porous culture plate, and the culture liquid circulation channels are communicated with the culture holes. The culture solution circulation channel comprises an injection port, an input channel, a discharge channel and a discharge port, the injection port is communicated with the input channel, and the discharge channel is communicated with the discharge port; the porous culture plate comprises a plurality of culture holes and a plurality of culture solution circulation channels, each culture solution circulation channel is communicated with an injection port and a discharge port of at least one culture hole, the transwell module comprises more than one transwell chamber, the transwell chambers are matched with the culture holes and placed on the culture holes, and the upper cover module is used for sealing and covering the porous culture plate. According to the liquid core device, a traditional culture plate and a micro-fluidic technology are combined, high-throughput culture of the organoid is achieved, and the homogeneity of the organoid culture tissue is improved due to the fact that a parallel multi-group organoid culture mode is adopted.

The invention discloses a culture method of high-activity human urine-derived stem cells, which relates to the technical field of biological medicine, and is technically characterized by comprising the following steps: collecting urine samples of pregnant and lying-in women, coating a culture plate with gelatin, inducing adherence, performing passage amplification, performing immunofluorescence identification, performing function detection and other optimized conditions, and separating and culturing the stem cells. Compared with urine-derived stem cells of normal adults, the stem cells derived from pregnant women have stronger activity, higher dryness and faster multiplication capacity. The invention provides a novel and superior cell source for tissue engineering and regenerative medicine.

The invention relates to the technical field of preparation of organoids, and discloses a method for regulating and controlling human induced pluripotent stem cells to differentiate into kidney organoids, which comprises the following steps: resuspending a cell cluster formed by human induced pluripotent stem cells by using a basic differentiation culture solution containing a GSK-3beta inhibitor, inoculating the cell cluster into an ultralow adsorption cell culture plate, and starting to culture, marking the time as the 0th hour of differentiation; and after culturing for 70-76 hours, replacing the basic differentiation culture solution containing the GSK-3beta inhibitor with a basic differentiation culture solution containing a Knockout TM serum substitute (KSR), and continuing to carry out differentiation culture. By adopting the method disclosed by the invention, the human induced pluripotent stem cells can be efficiently, quickly, simply, conveniently and stably subjected to directional induced differentiation into kidney organs containing podocytes, renal tubules and other structures, and the time for constructing a kidney in-vitro model can be shortened, so that the research on kidney diseases, kidney development and the like can be greatly assisted; and a powerful platform is provided for kidney toxicity assessment.

The invention relates to an extraction and culture method of urine-derived stem cells, and belongs to the field of cell biology and regenerative medicine. The method comprises the following steps: coating a culture plate with a gelatin solution; collecting clean midstream urine of healthy people and adding antibiotics; centrifugally separating the cell precipitate; washing with a phosphate buffer solution; re-suspending the precipitate by using a urine stem cell complete culture medium for primary culture; and after the cells grow to a certain fusion degree, carrying out passage amplification. The urine stem cell complete medium comprises a basic medium, fetal calf serum, epidermal growth factors, platelet-derived growth factors and basic fibroblast growth factors. By adopting the method disclosed by the invention, the urine-derived stem cells can be efficiently and stably separated from urine, and the obtained cells have high multiplication capacity, good stemness maintaining capacity, multidirectional differentiation potential and immunoregulation function, and can provide an ideal seed cell source for treating diseases such as diabetic erectile dysfunction.

The invention provides a cell culture plate, and belongs to the technical field of biological experiment equipment. The multi-channel pipettor solves the problem that in the prior art, sample adding or sampling of a multi-channel pipettor with smaller sample spacing cannot be realized on a cell culture perforated plate with a larger single-hole area. The cell culture plate comprises a plate body provided with a plurality of culture holes distributed in rows and columns, the plurality of culture holes are divided into a plurality of groups of culture holes in sequence by taking two columns as one group, and the positions of two adjacent and parallel culture holes in each group of culture holes are distributed in a staggered manner. The multi-channel pipettor has the advantage that sample adding or sampling of a multi-channel pipettor such as an 8-channel pipettor or a 12-channel pipettor with smaller sample spacing can be realized on a cell culture perforated plate with a larger single-hole area.

The utility model relates to the technical field of life science, and provides a photographing and image analyzing system for a culture plate, which comprises a support device, an image acquisition device, a moving device and a control device, and is characterized in that the support device is used for placing the culture plate; the moving device is arranged above the supporting device; the image acquisition device is connected with the moving device and is suitable for shooting the culture plate hole by hole under the driving of the moving device so as to acquire an image of a sample in the culture plate; the control device is electrically connected with the image acquisition device and the moving device, the control device is used for controlling the moving device to move so as to drive the image acquisition device to shoot holes one by one, and the control device is further used for analyzing and processing images acquired by the image acquisition device. The photographing and image analyzing system for the culture plate can meet the high-throughput experiment requirement and cannot generate a parallax effect.

The utility model discloses an edible fungus incubator networking alarm device, which relates to the technical field of edible fungus cultivation, comprises a box body, and is characterized in that a box door is arranged on one side of the box body, a sealing gasket is arranged on one side, close to the box body, of the box door, and three mounting blocks are arranged on each of two sides of the inner wall of the box body. And draining plates are arranged among the three groups of mounting blocks. The sealing effect between the box body and the box door can be improved through the arranged sealing gasket, external dust and impurities are prevented from entering the box body to influence thallus embryos, when the box body needs to be atomized and humidified, some small water drops in the culture groove are gathered together, water can fall into the draining groove through the draining holes, and therefore the thallus embryos are prevented from being damaged. Therefore, some water can be prevented from being deposited in the culture tank for a long time to influence thalli in the culture tank, the quality of produced edible mushrooms is ensured, the culture plate can be taken out by pulling the handle, the water collected in the draining tank can be conveniently recycled, and waste of water resources is avoided.

1. The name of the design product: micro-well array tumor organoid culture plate. 2. The use of the design product: for tumor organoid culture, tumor drug screening and personalized treatment. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: combination state diagram. 5. The right view of component 2 is the same as the left view of component 2, and the right view of component 2 is omitted; the bottom view of component 2 is the same as the top view of component 2, and the bottom view of component 2 is omitted. 6. Other circumstances that need to be explained Component explanation: component 1 is a well plate, and component 2 is a micro-well array chip.

The present invention relates to an automated machine for processing culture plates, comprising an operating unit for receiving culture plates in continuous succession and performing a series of successive treatments on each culture plate. The operating unit comprises a plurality of stationary processing stations (PT1-PT8) distributed at a predetermined interval around an axis (X2) and capable of being activated at least partially simultaneously. It also comprises a rotational drive device (DER) for the culture plates relative to the processing stations, having housings distributed according to the interval of the processing stations such that a rotation of the drive device around the axis brings a given culture plate successively to each of the processing stations. Figure 1

The invention discloses a culture method of in-vitro uniform monolayer cells of prawns, and belongs to the technical field of biology. The method comprises the following steps: (1) obtaining lymphatic tissues of a last-stage prawn at the early stage of shelling, completely stripping an outer membrane to obtain internal tissues, and cutting the internal tissues into small tissue blocks; (2) cleaning the small tissue blocks, inoculating the small tissue blocks to a culture plate coated with a gelatin solution, adding a complete culture solution into an inoculation system at intervals within 12 hours after inoculation for culture, replacing the complete culture solution 24-48 hours after inoculation, and inducing tissue cells to continuously migrate to form an in-vitro cell layer; (3) blowing and beating the cell culture to obtain a cell suspension, and centrifuging to obtain a cell monolayer; and (4) suspending the cell monolayer by adopting a complete culture solution, then inoculating the cell monolayer into a culture plate coated with a gelatin solution, and culturing to obtain the in-vitro uniform monolayer cells of the prawns. According to the method, the prawn monolayer cells which are long in in-vitro survival time, uniform and stable and are cultured in vitro can be obtained, and an important tool is provided for related virology research.

The utility model discloses a tumor cell scratching device, which relates to the technical field of cell experiments and comprises a worktable body, a protective baffle is fixedly mounted at the center of the upper surface of the worktable body, and a scratching component is arranged above the protective baffle. The motor is started, the output end of the motor drives the first gear to rotate, the first gear drives the double-face circular gear ring meshed with the first gear to rotate, and therefore the four second gears meshed with the inner side teeth of the double-face circular gear ring are driven to rotate; when each group of second gears rotate, a correspondingly meshed rack is driven to move in the worktable main body, and when the rack moves, a fixed rod fixed at the top of the rack is driven to synchronously move towards the direction of the culture plate, so that a telescopic rod and an L-shaped positioning block are driven to move towards the direction of the culture plate, and on one hand, the culture plate is quickly centered and accurately positioned; on the other hand, the culture plates with different sizes can be positioned, and the practicability of the device is improved.

The utility model relates to a cell co-culture experimental device easy to change liquid, which comprises a culture plate, and a liquid inlet device is arranged on the side wall of the culture plate close to the bottom so as to discharge new liquid into the culture plate; a liquid discharging device is arranged at the bottom of the culture plate and is used for discharging old liquid in the culture plate. The liquid inlet device comprises a liquid inlet pipe arranged on the side wall of the culture plate, the liquid inlet pipe is obliquely arranged, the liquid inlet direction inclines to the bottom of the culture plate, and an arc-shaped protrusion opposite to a liquid inlet outlet of the liquid inlet pipe is arranged on a bottom plate of the culture plate. The liquid discharging device comprises liquid discharging pipes arranged at the bottom of the culture plate, and the multiple liquid discharging pipes intersect and communicate with a liquid collecting pipe arranged at the bottom of the culture plate. According to the cell co-culture device, cell pollution can be avoided on the basis of quickly realizing cell liquid change, and meanwhile, the cell co-culture process is simplified.

The invention belongs to the field of medicines, and particularly relates to an in-vitro evaluation model for promoting skin wound healing based on a VPA-Exo drug-loaded exosome as well as a construction method and application of the in-vitro evaluation model. The construction method of the in-vitro evaluation model for promoting skin wound healing comprises the following steps: S1, after passage of resuscitated HSF cells and HUVEC cells, taking HSF cells or HUVEC cell suspension in a logarithmic phase, inoculating the HSF cells or HUVEC cell suspension on a culture plate, and culturing on a real-time label-free cell analyzer for 20-28 hours; and S2, then adding VPA-EXO with the concentration of 12.5 mu g / mL-400 mu g / mL to act on the HSF cells or the HUVEC cells.

The invention relates to the field of immune cell chemistry, and discloses an immune cell chemical staining instrument and a use method thereof.The immune cell chemical staining instrument comprises a workbench, a sample bearing unit, a temperature control unit, a liquid treatment unit and a dynamic uniform mixing unit, and cover glass in holes of a cell culture plate of the sample bearing unit can bear samples; a reagent in the reagent bin can be added into a hole of the cell culture plate by a pipetting arm of the liquid treatment unit, the temperature control unit can control the temperature of the reagent in the hole of the cell culture plate, and the dynamic uniform mixing unit can drive the cell culture plate to reciprocate, so that liquid in the hole of the cell culture plate is uniformly mixed. Reagents are automatically added through the liquid treatment unit, the experiment operation efficiency is improved, pollution caused by manual intervention is avoided, the reagent temperature is controlled through the temperature control unit, and the experiment accuracy is improved; meanwhile, the dynamic uniform mixing unit is used for driving the cell culture plate to reciprocate, so that the reagent incubation uniform mixing effect is enhanced.

The utility model discloses an incubator for food detection, which comprises an incubator body, an incubator door arranged on the side surface of the incubator body, a control box fixedly connected with the top of the incubator body, a rotating shaft rotationally connected with the outer side of the rotating shaft, a worm gear fixedly connected with one end of the rotating shaft, a rotating plate fixedly connected with one end of the rotating shaft, and a sliding rod fixedly connected with the side surface of the rotating plate. A sliding groove is formed in the side face of the mounting plate, a containing groove is formed in the side face of the mounting plate, a containing plate is arranged on the side face of the mounting plate, a rectangular groove is formed in the side face of the containing plate, and a pull rod is arranged in the rectangular groove. Through the rotating shaft, the worm gear, the rotating plate, the sliding rod, the worm and the mounting plate, the height of the mounting plate is adjusted, culture trays with different heights can be placed, through the placing plate, the pull rod, the spring, the connecting plate, the hinged plate, the inserting rod and the inserting hole, the placing plate can be conveniently disassembled, and the convenience of actual use is enhanced.

The utility model relates to the technical field of sound barriers, and discloses a plug-in type cultural sound barrier which comprises a base, the upper surface of the base is connected with a lower barrier, the upper surface of the lower barrier is connected with a cultural plate, the upper surface of the cultural plate is connected with an upper barrier, and the upper surface of the base is connected with a supporting assembly. The lower barrier, the culture plate and the upper barrier are all located between the supporting assemblies, the upper ends of the supporting assemblies are connected with the two anti-pressing assemblies, and the other ends of the two anti-pressing assemblies are connected with the two sides of the upper end of the culture plate correspondingly. According to the plug-in culture sound barrier, the supporting assemblies are arranged on the base, the lower barriers, the culture plates and the upper barriers are arranged between the supporting assemblies, and the supporting assemblies are wrapped inside, so that when the lower barriers, the culture plates and the upper barriers are used, the lower barriers, the culture plates and the upper barriers can be spliced; therefore, the lower barrier, the culture plate and the upper barrier are prevented from being hindered by the supporting assembly while the supporting effect is not affected.

The invention relates to the technical field of plant culture equipment, in particular to heat-preservation mushroom strain culture equipment which comprises a bottom plate, supporting rods are symmetrically and fixedly connected to the upper surfaces of the two sides of the bottom plate, and the heat-preservation mushroom strain culture equipment further comprises a culture mechanism fixedly installed at the tops of the supporting rods; the heat preservation mechanism is fixedly mounted in the cultivation mechanism. Heat is uniformly diffused into the cavity of the cultivation box above the cultivation plate through the heat dissipation holes in the heat dissipation cover and then is guided out through the air outlet in the top cover, and the bottom of the cultivation box is in a micro-repressing state due to rising of hot air, so that cold air starts to be sucked inwards from the air inlet of the bottom cover, and the temperature of the cultivation box is increased. And cold air is heated by the heating coil and then enters the heat dissipation cover to realize heat circulation, so that the flowing effect of air in the box body is kept while heat preservation is performed on the culture box, and the culture environment of strains is greatly improved.

The invention discloses a laboratory culture dish fixing frame and a tray thereof, the laboratory culture dish fixing frame comprises a tray body, a groove formed in the tray body and used for placing a culture dish and a grabbing part corresponding to the groove, the grabbing part comprises a hollow point arranged at the edge of the groove, and the tray comprises a tray body. A step-shaped mounting groove matched with the step-shaped slot is formed in the inner wall of the tray body; according to the invention, the groove is formed, and the grabbing part for conveniently grabbing the culture dish in the groove is arranged in the edge area of the groove, so that the culture dish is convenient to take and place, the problem that the culture dish or the culture plate is inconvenient to take and place in the experiment process is avoided, in addition, the culture dish placing area is used for placing the culture dish, and a groove type arrangement mode is adopted, so that the culture dish or the culture plate is convenient to take and place. The sealing film is arranged between the culture dishes, so that an interval exists between the culture dishes, and the problems that the sealing film possibly adheres to other culture dishes or culture plates, toppling or culture solution leakage is caused, and experimental results are affected are solved.

Disclosed is a chemically defined airway basal stem cell culture medium. The provided culture medium involves a simple preparation method and high safety, and does not require a feeder layer or a matrigel-coated culture plate. By means of the culture medium, primary cultured airway basal stem cells achieve good stability; and after multiple passages, the normal morphology of the basal stem cells can be maintained and good differentiation potential can be maintained. In addition, the culture medium has clear components, and can meet both scientific research-grade and clinical-grade applications of airway basal stem cells.

The utility model relates to the technical field of cell culture, and discloses a culture plate for an organoid, which comprises a plate body, a plurality of culture holes and a plurality of cells, the cover body is detachably arranged on the plate body and used for forming a closed space, and the culture holes are located in the closed space formed by the plate body and the cover body; wherein each culture hole is internally provided with a cell culture chamber, and a layer of sealing gasket for independently sealing each culture hole is arranged between the plate body and the cover body. According to the utility model, the culture plate can achieve effective sealing stability during transportation, so that the cell culture plate can be conveniently transported among different laboratories.

The present invention relates to the technical field of cell culture, and specifically to a cell culture device for immune cell amplification, which includes an incubator, a culture plate disposed inside the incubator, a culture bottle disposed above the culture plate, a bottle-turning mechanism fixedly disposed on the upper surface of the culture plate and driving the culture bottle to rotate by 90 degrees, a medicine-adding mechanism for injecting nutrient solution into the culture bottle, a liquid-sucking mechanism for sucking out the nutrient solution from the culture bottle, a substrate fixedly disposed on the rear inner wall of the incubator and located below the culture plate, and an active precipitation mechanism fixedly disposed on the substrate and alternately driving the culture plate to perform upward acceleration movement and downward uniform movement. The present invention adopts the active precipitation mechanism to make the culture bottle in a superheavy state, accelerating the precipitation of cells in the culture bottle. The medicine-adding mechanism can automatically supplement the nutrient solution into the culture bottle, and the liquid-sucking mechanism can suck out part of the old nutrient solution in the culture bottle, facilitating semi-quantity replacement of the cells.

The invention discloses a multilayer adherent cell culture reactor and a use method. The culture reactor comprises a shell, a plurality of layers of cell culture plates are arranged in the shell in an array manner; and the liquid inlet is used as an inlet of a cell suspension. The liquid outlet is an outlet of the culture medium; the air holes are formed in the upper surface and the lower surface of the shell. And the quality control chamber is communicated with the inner cavity of the shell. The use method comprises the following steps: uniformly mixed cells and a culture medium enter the inner cavity of the shell through the liquid inlet for positive culture; after front culture is completed, the multi-layer adherent cell culture device is inverted; uniformly mixed cells and a culture medium enter the inner cavity of the shell through the liquid inlet for reverse culture. By arranging the multiple layers of cell culture plates with double surfaces capable of culturing, the cell culture area is greatly increased, the space utilization rate is improved, the use amount of a culture medium is reduced, the prepared quality control chamber is communicated with the inner cavity of the shell, and the cell growth state is conveniently, rapidly and accurately observed on the premise of not polluting the internal environment and not damaging the culture stability through isolation of the partition opening.

The utility model discloses a kind of water culture devices for yunnan rhizoma polygonati breeding, it is related to yunnan rhizoma polygonati cultivation technical field, including culture rack, the culture rack is slidably connected with multiple water storage channels, and the water storage channel upper end is provided with culture plate;Multiple planting holes are evenly provided in the culture plate, and the culture plate upper end is connected with multiple fixed components, and fixed component is located planting hole outer end.The utility model design structure is reasonable, it can simultaneously carry different batches, different genotypes yunnan rhizoma polygonati breeding material, while independent water supply head, gate plate can individually adjust the water supply of each water storage channel, water supply frequency, realize the accurate management of "one tank one strategy", satisfy the individualized demand of different breeding material, to improve the success rate of breeding, can avoid the problem of "excessive supply" or "insufficient supply" caused by unified water supply, accurately match the demand of each growth stage of yunnan rhizoma polygonati, save water resource, nutrient solution and other consumables, reduce breeding cost.

The present invention discloses a drug sensitivity detection microwell plate, and relates to the technical field of microbiological detection, the drug sensitivity detection microwell plate comprises a bottom plate and a cover plate, the top of the bottom plate is integrally fixed with a culture plate body, and the height difference between the outer edge of the culture plate body and the bottom plate forms a step. In the using process of the culture plate, an operator only needs to execute the action of palm pressing, synchronous liquid supplementing operation on all the hole sites in the culture plate body can be synchronously achieved, the liquid supplementing efficiency is remarkably improved, the process of continuously executing the actions of turnover liquid supplementing, vertical liquid supplementing and return pressing is extremely simplified, and the operation efficiency is greatly improved. The method not only can effectively reduce the learning cost of a user and is beneficial to large-scale mass production and popularization, but also greatly shortens the time of cell liquid change compared with the traditional pipette liquid change, can effectively avoid long-time dehydration death of cells caused by overlong liquid change time, improves the quality of the cultured cells, and reduces the production cost of the cells. And the accuracy and success rate of the experiment are improved.

The invention discloses intelligent feed processing feeding equipment capable of reducing air pollution, and relates to the technical field of feed production, the intelligent feed processing feeding equipment comprises a transmission mechanism, the transmission mechanism is mounted on one side of a fermentation tank, the fermentation tank is filled with silage raw materials, the silage raw materials are subjected to fermentation treatment in the fermentation tank, and the transmission mechanism is mounted on the other side of the fermentation tank; carbohydrates in the raw materials are decomposed through microorganisms, silage raw material fermentation products are used as feed, the feed is put onto the transmission mechanism from the fermentation tank and conveyed through the transmission mechanism, and the top end of the fermentation tank is communicated with a conveying pipeline. Carbon dioxide generated by silage fermentation is collected through the conveying pipeline and directionally conveyed to the algae culture plate through the ventilation shell, the carbon dioxide is converted into organic matter through algae photosynthesis, the carbon dioxide recovery rate is increased, and it is avoided that the carbon dioxide is directly discharged to pollute the atmosphere; meanwhile, the equipment is closed in the whole process and is matched with terminal dust removal treatment, the dust emission concentration is reduced, unorganized emission is greatly reduced, and the environment-friendly requirement is met.

The utility model relates to a fine line device for an experiment for detecting cell migration ability, which comprises a fine line main body, the adhesive sheets are arranged at the two ends of the fine line main body, and adhesive protective layers are arranged on the adhesive sheets; and the two ends of the fine line main body exceed the adhesive sheet. The thin line main body with the structure can be conveniently adhered to a culture plate through the adhesive sheet and is also convenient to tear off, and a blank area is left after the thin line main body is torn off and is used for observing and recording the cell migration capability. The utility model provides a fine line device for an experiment for detecting cell migration ability, which is detachable, simple and convenient to operate, and capable of ensuring the consistency of scratch width and reducing damage to scratch edge cells and the bottom surface of a culture plate. The problems that scratches engraved by a gun head are inconsistent in width, and damage to cells and the bottom face of a culture plate is large are solved.

The utility model discloses a cell culture unit and a cell culture plate comprising the cell culture unit, which belong to the field of cell culture and comprise culture tubes and caps arranged on the culture tubes. The outer part of the open end of the culture tube is sleeved with a positioning ring clamped with the cap, and an observation hole is formed in the central area of the cap. According to the cell culture unit and the cell culture plate containing the cell culture unit, the cell culture unit adopts a modular design, so that each culture unit can be independently subjected to sampling inspection and replacement, the sampling inspection cost is reduced, and the design that the observation hole is matched with the micron-sized pore membrane allows a user to observe the cell growth condition without opening the cap, so that the cell culture efficiency is improved. The risk of pollution is reduced, due to the design of the cell culture plate, especially the arrangement of the shading cover and the ventilation holes, the optimal growth environment is provided for cell types sensitive to light, and meanwhile gas exchange and dust prevention are ensured.