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Micropatterned hydrogel coating, its preparation method and use

A micropatterning and hydrogel technology, applied in biochemical equipment and methods, biochemical instruments, nervous system cells, etc., can solve the problems of difficult comparison of cell culture results, etc., and achieve easy-to-obtain raw materials, simple preparation methods, and wide application range wide effect

Active Publication Date: 2013-10-16
广州永翔科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the composition of different batches of animal-derived substrates such as matrigel and collagen is uncertain, and the results of cell culture are difficult to compare, and the size of the formed cell spheres is also relatively random.

Method used

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  • Micropatterned hydrogel coating, its preparation method and use
  • Micropatterned hydrogel coating, its preparation method and use
  • Micropatterned hydrogel coating, its preparation method and use

Examples

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preparation example Construction

[0042] The preparation steps of the hydrogel solution: adding the raw materials used to prepare the micropatterned hydrogel coating as a solute into distilled water at 70°C to 90°C, fully stirring and dissolving to form water with a weight percentage of 1 wt% to 10 wt% For the gel solution, keep the temperature of the hydrogel solution within the range of 70°C to 90°C.

[0043] Molding step of the hydrogel coating: select a suitable carrier for carrying the micropatterned hydrogel coating, and evenly spread the prepared hydrogel solution at a temperature of 70°C to 90°C on the carrier Lower the temperature to 40° C. to 60° C., and when the hydrogel solution forms a hydrogel viscous colloid, press the mold on the hydrogel viscous colloid and print the obtained dot matrix pattern. The raw material includes at least one of carrageenan, locust bean gum, gum arabic, xanthan gum, algin, fucoidan, xylan, agarose, mannan, galactomannan, and glucomannan kind.

[0044] The mold is a s...

Embodiment 1

[0048] Preparation of 6-well cell culture plates with micropatterned hydrogel coatings included.

[0049] A standard 6-well flat-bottomed cell culture plate made of ordinary polystyrene, used as a carrier for hydrogel coating preparation after sterilization. First, several powdered solids such as gum arabic, xanthan gum, agarose, and glucomannan were mixed at a mass ratio of 2:1:6:1, and added to distilled water at 90 °C to make the solute mass fraction about 4 wt%. Prepare the hydrogel solution, fully stir to dissolve and keep the hydrogel solution at 90°C. Then, take the hydrogel solution in the above incubation and add it to each well of the prepared 6-well plate. The amount added to each well is about 900 μl. The amount added to each well depends on the volume of a single well of the culture plate or the culture dish. The larger the volume, the amount added should be increased accordingly. For example, the amount added to each well of a 6-well plate should be greater than...

Embodiment 2

[0051] Preparation of Petri dishes with micropatterned hydrogel coatings included.

[0052] A 35 mm standard petri dish made of ordinary polyethylene was used as a carrier for micropatterned hydrogel coating preparation after sterilization. A 90°C hydrogel solution was prepared as described in Example 1, and about 800 μl of the 90°C hydrogel solution was added to a petri dish. The molding curing and demoulding steps of the hydrogel coating are similar to Example 1, the difference is that a mechanical pressurizing device is used in this example to connect the mold of the round-bottom stamp for automatic pressurization, such as Figure 5a and 5b . The device can greatly improve production efficiency and reduce the influence of human factors, Figure 5c Shown is a formed 35 mm cell culture dish containing a micropatterned hydrogel coating.

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Abstract

The invention discloses a micropatterned hydrogel coating, its preparation method and use. The preparation method comprises the following steps: using one of carrageenan, locust bean glue, Arabic gum, xanthan gum, brown algae glue, brown algae sugar glue, xylan, agarose, mannan, galactomannans and glucomannans as a raw material to prepare a hydrogel solution first; spreading the hydrogel solution on a carrier such as a three-dimensional cell ball culture dish or a culture plate and the like; obtaining the coating with lattice patterns uniformly distributed on the surface through moulding and solidification demoulding. The micropatterned hydrogel coating may be used to produce culture products, and the culture products may enable a plurality of cells to propagate in a uniform multicellular ball way without other exogenous power so that the batch preparation of three-dimensional multicellular balls may be realized. The raw materials are easily available, quite low in cost, and natural and toxicity-free; the prepared coating belongs to a transparent nondiffusible coating; the growth of the cells in an animal body is highly simulated; and the coating has excellent stability and biological activity and is applicable to most cell culture products.

Description

technical field [0001] The present invention relates to a hydrogel coating and its preparation method and use, in particular to a micropatterned hydrogel coating and its preparation method, and its application to carriers such as three-dimensional cell sphere culture dishes or culture plates This kind of culture product can promote the proliferation of various cells in the form of uniform multicellular spheres without other external power, and can be used to prepare three-dimensional multicellular spheres in batches. Background technique [0002] Animal cell culture refers to taking relevant tissues from the animal body, dispersing them into individual cells and placing them in a suitable medium to allow these cells to grow and proliferate. Animal cell culture is an essential technology in biological and medical research, and it is widely used in important fields such as antibody preparation, drug screening, toxicity monitoring and detection, stem cell regeneration, tissue a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L5/00C08L5/12C08J3/075B29C59/02C12M3/00C12N5/0797C12N5/0783
Inventor 赵鹏文学军李寅莹刘静
Owner 广州永翔科技发展有限公司
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