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5 results about "Amphibian" patented technology

Amphibians are ectothermic, tetrapod vertebrates of the class Amphibia. Modern amphibians are all Lissamphibia. They inhabit a wide variety of habitats, with most species living within terrestrial, fossorial, arboreal or freshwater aquatic ecosystems. Thus amphibians typically start out as larvae living in water, but some species have developed behavioural adaptations to bypass this. The young generally undergo metamorphosis from larva with gills to an adult air-breathing form with lungs. Amphibians use their skin as a secondary respiratory surface and some small terrestrial salamanders and frogs lack lungs and rely entirely on their skin. They are superficially similar to lizards but, along with mammals and birds, reptiles are amniotes and do not require water bodies in which to breed. With their complex reproductive needs and permeable skins, amphibians are often ecological indicators; in recent decades there has been a dramatic decline in amphibian populations for many species around the globe.

Construction method and application of ovary cell line of vijaynathella emeiensis

The application discloses a construction method and application of an ovary cell line of Quasipaa spinosa. The method comprises the following steps: selecting metamorphosis period larva ovary tissue, disinfecting and obtaining the ovary tissue, and then using collagenase I digestion solution to digest the ovary tissue at 26 DEG C for 1 hour; after the tissue block adheres to the wall, using a low-osmotic-pressure complete culture medium to carry out primary culture at 25-27 DEG C, wherein the culture medium is 60% diluted DMEM / F12 culture medium containing 10%-15% fetal bovine serum; when the cell confluence degree is higher than 70%, using trypsin-EDTA to digest the cells for 1-2 minutes to subculture, and the subculture culture medium contains 30% cell conditional culture medium; and finally, the cells are cryopreserved through programmed cooling. The ovary epithelial cell line of Quasipaa spinosa is successfully established for the first time, the survival rate of the cells after recovery is high, and the karyotype is stable. The constructed cell line can be used for amphibian reproductive biology research, compound screening and in-vitro conservation of germplasm resources.
Owner:HUAZHONG NORMAL UNIV

Formulations and methods for controlling invasive amphibians

PendingAU2020297182B2AmphibianZoology
The present invention relates to formulations and methods for humanely controlling invasive amphibian populations, and in particular the cane toad, comprising 2-phenoxyethanol and ethanol and / or isopropanol as carrier.
Owner:ANIMAL CONTROL TECH (AUSTRALIA) PTY LTD

Mud bucket with integrated exit area for amphibians and / or other small animals

UndeterminedDE202026001871U1Small animalAmphibian
A silt bucket for a road drain, comprising a wall and a bottom area, characterized in that at least one interior area of ​​the silt bucket has one or more integrated exit areas accessible from the interior near the bottom and comprising a climbable structure, wherein the climbable structure is dimensioned and its structural sections are arranged relative to each other in such a way as to enable an amphibian and / or other small animal located in the silt bucket to grasp, support itself and ascend in a directed manner to an upper edge, exit or transition area of ​​the silt bucket, and wherein drainage and / or overflow openings with a free passage cross-section larger than that of the climbable structure are arranged outside the respective exit area.
Owner:HATIBA DEUTSCHLAND GMBH

Multi-layer river channel wood pile revetment

The utility model relates to ecological prevention and control technical field especially relates to a multilayer river course wood pile revetment, including water retaining area and revetment area, the revetment area sets up at the outside of river bank, is equipped with the diaphragm between the inside of revetment area and the outside of river bank, water retaining area sets up in the river course of revetment area outside, water retaining area includes first wood pile, water retaining area gravel layer, water retaining area soil layer and submerged plant, the utility model discloses through setting up the double -deck protection of water retaining area and revetment area, has improved the water flow impact resistance of river bank significantly, and water retaining area and revetment area fill gravel, soil and plant and plant, provide the continuous ecological corridor for fish spawning, benthic animal habitat, amphibian migration and bird foraging.
Owner:江西省 中国科学院庐山植物园 +4