28 results about "Amphibian" patented technology

The application discloses a construction method and application of an ovary cell line of Quasipaa spinosa. The method comprises the following steps: selecting metamorphosis period larva ovary tissue, disinfecting and obtaining the ovary tissue, and then using collagenase I digestion solution to digest the ovary tissue at 26 DEG C for 1 hour; after the tissue block adheres to the wall, using a low-osmotic-pressure complete culture medium to carry out primary culture at 25-27 DEG C, wherein the culture medium is 60% diluted DMEM / F12 culture medium containing 10%-15% fetal bovine serum; when the cell confluence degree is higher than 70%, using trypsin-EDTA to digest the cells for 1-2 minutes to subculture, and the subculture culture medium contains 30% cell conditional culture medium; and finally, the cells are cryopreserved through programmed cooling. The ovary epithelial cell line of Quasipaa spinosa is successfully established for the first time, the survival rate of the cells after recovery is high, and the karyotype is stable. The constructed cell line can be used for amphibian reproductive biology research, compound screening and in-vitro conservation of germplasm resources.

The invention provides a molecular marker primer for tailed amphibians and application of the molecular marker primer. The nucleotide sequence of the primer is ISSRSEQ6; and the sequence of the gene is 5 '-AATCAATCAATCAATCAATCAATCC-3'. The primer can be used for carrying out PCR (Polymerase Chain Reaction) amplification on genome DNA (Deoxyribose Nucleic Acid) of tailed amphibians to obtain SNPs.

The utility model belongs to the field of animal breeding, and particularly relates to an amphibian automatic breeding modularization system. Comprising a box body, the box body comprises a bottom plate fixedly arranged at the bottom, a supporting plate is transversely and vertically arranged at the top of the bottom plate, a feeding area is arranged on one side of the supporting plate and abuts against the inner side of one side wall of the box body, and an automatic feeding device is arranged on the outer side of the side wall, abutting against the feeding area, of the box body in a penetrating mode; a top plate of the feeding area can be automatically flushed to prevent food residues from remaining, and the other side of the supporting plate is provided with a water area for animals to survive; a top cover is detachably arranged at the top of the box body, a control unit is arranged at the bottom of the top cover, and a full-spectrum illuminating lamp, a camera, an ultraviolet lamp and a regulation and control unit are detachably arranged at the bottom of the top cover and used for monitoring and adjusting the internal environment.

1. The name of the design product: biological shelter. 2. The use of the design product: for outdoor ground activity biological (such as low-foot poultry, amphibians) to provide space to hide from natural enemies, habitat and breeding. 3. The design points of the design product: in shape. 4. The picture or photo that best shows the design points: perspective view 1.

The present invention relates to formulations and methods for humanely controlling invasive amphibian populations, and in particular the cane toad, comprising 2-phenoxyethanol and ethanol and / or isopropanol as carrier.

An animal proof fence (1) comprising one or several panel elements (2) which are provided to extend upright to form a barrier, wherein the fence (1) comprises an upper zone (A) wherein at the height of the upper zone (A) the one or several panel elements (2) comprise a smooth unperforated surface on which reptiles, such as snakes, amphibians and small mammals cannot climb and a lower zone (B) wherein at the height of the lower zone (B) the one or several panel elements (2) comprise perforations allowing view through the fence (1).

The utility model discloses an amphibian breeding device which comprises a device body, an air cylinder is connected to the back face of the device body, a telescopic rod is slidably connected to the inner wall of the air cylinder, a scraping rod is connected to the end, away from the air cylinder, of the telescopic rod, a sealing frame is connected to the outer surface of the scraping rod, and a sealing plate is hinged to the front face of the device body. The front face of the device body is connected with an impurity box, the front face of the impurity box is hinged to a baffle, and the front face of the baffle is connected with a handle. The air cylinder is arranged to drive the telescopic rod to push impurities out of the device through the scraping rod, the impurities at the bottom are automatically scraped and pushed, the phenomenon that bacteria are generated due to excessive impurities at the bottom can be effectively prevented, and therefore cleanliness and health of water are maintained; the aim of only removing impurities without replacing all water is achieved, water resources are greatly saved, the baffle is opened by pulling the handle for cleaning, the water does not need to be manually drained for cleaning, and the labor intensity is reduced.

A composition for managing, controlling, neutralizing, killing, or repelling an amphibian or reptile comprises at least one active ingredient comprising an effective amount of thymol or a derivative thereof and an effective amount of eugenol or a derivative thereof. The composition is also useful for protecting, guarding, or repelling a pet.

A lure is disclosed. The lure comprises a head, a tail, a first channel extending inside the head to the tail, and a pair of hind legs extending from the tail, the pair including a first leg having a first foot and a second leg having a second foot, the first foot being connected to the second leg and forming a foot body having a second channel. The lure has a line having a first end and a second end, the second end being provided through the first channel and the second channel. The second end is connected to the foot body. The lure can resemble a frog, an insect, or an amphibian.

The present disclosure describes compositions including activated amphibian skin having enhanced functional activity, methods of preparing the compositions, and methods of using the compositions for treating skin conditions and wound healing.

A silt bucket for a road drain, comprising a wall and a bottom area, characterized in that at least one interior area of ​​the silt bucket has one or more integrated exit areas accessible from the interior near the bottom and comprising a climbable structure, wherein the climbable structure is dimensioned and its structural sections are arranged relative to each other in such a way as to enable an amphibian and / or other small animal located in the silt bucket to grasp, support itself and ascend in a directed manner to an upper edge, exit or transition area of ​​the silt bucket, and wherein drainage and / or overflow openings with a free passage cross-section larger than that of the climbable structure are arranged outside the respective exit area.

The invention relates to the technical field of artificial breeding of amphibians, in particular to an ecdysis promoting method for artificially imitating wild breeding of toads and application, according to the ecdysis promoting method, the toads are soaked in a soaking solution containing iodophor, the effective iodine concentration in the soaking solution is 0.1-0.2%, and the soaking time is 10-25 minutes. By the adoption of the ecdysis promoting method, the ecdysis period is greatly shortened, and compared with natural ecdysis (12-15 days), the ecdysis period is shortened to 5-7 days, and efficiency is greatly improved; and the toad cortex quality and yield can be obviously improved, and the toad cortex integrity reaches 92% or above.

The invention discloses a construction method and application of an ovarian cell line of hapalogenys nitens. The method comprises the following steps: selecting a metamorphosis stage larva ovarian tissue, disinfecting and obtaining, and digesting for 1 hour at 26 DEG C by using collagenase I digestive juice; after the tissue block adheres to the wall, a low-osmotic-pressure complete medium is used for primary culture at the temperature of 25-27 DEG C, and the medium is a 60% diluted DMEM / F12 medium containing 10%-15% of fetal calf serum; after the cell confluence degree exceeds 70%, trypsin-EDTA (Ethylene Diamine Tetraacetic Acid) is used for digesting for 1-2 minutes for passage, and a passage culture medium contains 30% of a cell condition culture medium; and finally performing programmed cooling and cryopreservation. According to the method, the ovarian epithelial cell line of the hapalogenys nitens is successfully established for the first time, the cell recovery survival rate is high, and the karyotype is stable. The constructed cell line can be used for amphibian reproductive biology research, compound screening and germplasm resource in-vitro preservation.

The utility model relates to a small amphibious reptile observing and measuring box, and relates to the field of biological agriculture, the small amphibious reptile observing and measuring box comprises a rectangular box body, the surface of the rectangular box body is provided with a rectangular groove, an inner cavity of the rectangular groove is provided with a plug pin rod, a pin plate is arranged below the plug pin rod, and the surfaces of the plug pin rod and the pin plate are fixedly connected with battens. Through clamping between the column block and the round hole, the column block locks the position of the bolt rod in the inner cavity of the rectangular groove through the sliding rod and the column-shaped box, then the bolt rod changes the space of the inner cavity of the rectangular box body through the pin plate, and through the arrangement of the scale marks, the space of the inner cavity of the rectangular box body is changed. The size of small and medium-sized reptiles in the inner cavity of the rectangular box body can be conveniently observed, and the purposes that the space size is properly adjusted by moving the plug pins, the small amphibious reptiles are conveniently observed and measured, and meanwhile the small amphibious reptiles can be prevented from escaping to cause harm to personnel and the environment can be achieved.

The utility model relates to ecological prevention and control technical field especially relates to a multilayer river course wood pile revetment, including water retaining area and revetment area, the revetment area sets up at the outside of river bank, is equipped with the diaphragm between the inside of revetment area and the outside of river bank, water retaining area sets up in the river course of revetment area outside, water retaining area includes first wood pile, water retaining area gravel layer, water retaining area soil layer and submerged plant, the utility model discloses through setting up the double -deck protection of water retaining area and revetment area, has improved the water flow impact resistance of river bank significantly, and water retaining area and revetment area fill gravel, soil and plant and plant, provide the continuous ecological corridor for fish spawning, benthic animal habitat, amphibian migration and bird foraging.

The present application relates to a kind of abdominal spot frog antibacterial peptide Nv-CATH and its gene and application, the antibacterial peptide Nv-CATH is the circular polypeptide coded by the defense peptide gene of amphibian species unique species abdominal spot frog in China, molecular weight 3356.98 Dalton, isoelectric point 11.56, its amino acid sequence is as shown in SEQ ID NO:1.The gene (GenBank accession No.OP264074) of the precursor of the abdominal spot frog antibacterial peptide Nv-CATH is composed of 714 nucleotide sequences, its nucleotide sequence is as shown in SEQ ID NO:2, wherein the 373-522th nucleotide is the encoding gene of mature abdominal spot frog antibacterial peptide Nv-CATH.The application of abdominal spot frog antibacterial peptide Nv-CATH in preparing the therapeutic drug of the infection disease caused by hemolytic staphylococcus, enterococcus faecalis, staphylococcus aureus, klebsiella pneumoniae, acinetobacter junni and / or paratyphi A.The present application provides a new antibacterial peptide Nv-CATH with strong broad-spectrum antibacterial activity.

Small animal guidance system for ground-dwelling small animals, comprising a traffic route VW (2) of a two-way ramp culvert ZRD (7) crossing under it, which extends along a passage for receiving and guiding small animals migrating towards it, in particular amphibians, characterized in that on at least one side of the traffic route (2) in the area in front of the culvert (7) a demarcated, bundling and guiding bundling quarter BQ (4) is arranged and formed on a main crossing axis HQA (3), which defines a central path oriented towards the culvert ZRD (7) at a distance (A) between the bundling quarter (4) and the culvert ZRD (7).

The utility model belongs to the field of wetland ecological restoration, and discloses an artificial aquatic small organism inhabiting platform which comprises a buoyancy frame, the buoyancy frame comprises at least two connected buoyancy units, and the buoyancy units are connected with each other; the buoyancy unit comprises a buoyancy layer and a substrate layer for plant growth; three kinds of hygrophytes with different heights are planted on the substrate layer, and are matched with gravels and deadwood to build a multifunctional biological habitat; the device is easy to manufacture and reasonable in cost, solves the problems of atrophy of biological habitats and reduction of quality and diversity caused by hardening and homogenization of shorelines in artificial lakes, reservoirs and river channels, can quickly build high-quality and diversified biological habitats under the condition that the original shorelines are not transformed, meets the requirements of waterbirds, amphibians, fishes and benthonic animals, and has a wide application prospect. The reconstruction of a wetland food chain is facilitated, so that the biological diversity, the ecological system quality and the stability are effectively improved.

An animal proof fence (1) comprising one or several panel elements (2) which are provided to extend upright to form a barrier, wherein the fence (l) comprises an upper zone (A) wherein at the height of the upper zone (A) the one or several panel elements (2) comprise a smooth unperforated surface on which reptiles, such as snakes, amphibians and small mammals cannot climb and a lower zone (B) wherein at the height of the lower zone (B) the one or several panel elements (2) comprise perforations allowing view through the fence (1).

A lure is disclosed. The lure comprises a head, a tail, a first channel extending inside the head to the tail, a pair of hind legs extending from the tail and including a first leg having a first foot, a second leg having a second foot, the first foot being connected to the second foot forming a foot body having a second channel. The lure has a line having a first end and a second end, and the second end is provided through the first channel and the second channel. The second end is connected to the foot body. The lure may resemble a frog, insect, or amphibian.

The utility model provides an ecological gabion with a biological channel, which relates to the technical field of ecological protection and comprises a plurality of gabion cages, separators are arranged in the gabion cages and divide the gabion cages into a plurality of first compartments and a plurality of second compartments, the first compartments are filled with fillers, and the second compartments are filled with biological channels. The multiple second compartments are vacant, the adjacent second compartments communicate with each other to form inhabitation spaces, the multiple gabion cages are sequentially stacked from bottom to top, and the vertically adjacent inhabitation spaces communicate with each other to form a channel. The gabion cages are stacked from bottom to top, a biological channel from water to land can be formed, the gabion cages stacked up and down are arranged in the horizontal direction or combined with existing gabions to form a revetment, the maximum gradient of the revetment can be 90 degrees, precious shore space is saved, the gabion cages have good anti-scouring capacity and good ecological property, and the gabion cages stacked up and down can be used as the revetment. Amphibian landing channels and fish inhabiting places can be provided.

The present application provides a kind of softening vertical bulkhead construction sponge city's ecosystem, is composed of three basic units, plant bearing unit, fixed unit and plant fixer;Plant bearing unit is divided into two layers, and the upper layer support is fixed by steel welding connection between the lower layer, and the upper layer support is separated into multiple frame bodies by multiple horizontal steels in the frame, and the frame body is divided into multiple small square frames by multiple vertical steels;Multiple horizontal steels are welded in the lower layer support frame, and are arranged on the lower layer frame corresponding to the center line of the upper layer small square frame, to support the plant fixer.The application can improve the ecological environment of water body, enhance the self-purification of water body, provide habitat, breeding and refuge for various aquatic organisms and other amphibians, and ultimately form a healthy, multiple biological symbiotic water-land composite ecosystem.

The utility model discloses an assembly type spiral channel bank protection structure, which belongs to the field of bank protection structures, and comprises a plurality of protection components, each protection component comprises a dike block, one side of the dike block is provided with a first bulge, the other side of the dike block is provided with a first groove, the first bulge is matched with the first groove of the dike block of the adjacent protection component, and the first bulge is matched with the first groove of the dike block of the adjacent protection component. The first grooves are matched with the first protrusions of the embankment blocks of the adjacent protection assemblies. The fastening strips are connected with the embankment block, and a gap is formed between every two adjacent fastening strips; and the spiral channel body is propped between the embankment block and the plurality of fastening strips and is communicated with the gap. The utility model has the technical effects of not only being capable of adapting to water level change to provide inhabiting space for aquatic animals, but also being capable of serving as a buffer channel for land and water to provide a channel for amphibians.

The application discloses a kind of triflufensulfuron-methyl metabolite prediction and preparation method, it is related to pesticide detection technical field, including M-1, M-2 and M-3;Wherein the structural formula of M-1: the structural formula of M-2: the structural formula of M-3: the application further provides the preparation method of triflufensulfuron-methyl main metabolite M-1, M-2 and M-3, the determination of its synthetic method is conducive to further carry out triflufensulfuron-methyl main metabolite in the adsorption / desorption of environment, leaching, migration and conversion environmental behavior research and to aquatic organisms (zebrafish, black shell shrimp), terrestrial animals (red aposhechens earthworm) and amphibian (tadpole) acute toxicity and chronic toxicity mechanism research.

The invention discloses an amphibian unmanned aerial vehicle undercarriage structure, and relates to the technical field of unmanned aerial vehicle undercarriages. Comprising an annular frame mounted on an unmanned aerial vehicle body, connecting rods are fixed to the two sides of the annular frame, mounting bases are fixed to the bottom ends of the connecting rods, hollow bottom frames are mounted on the mounting bases, and the two hollow bottom frames are symmetrical about the circle center of the annular frame. By arranging the arc-shaped extension piece, the propeller assembly and other structures, the arc-shaped rack can be controlled to move according to requirements, then the arc-shaped extension piece is controlled to stretch out of the end of the hollow bottom frame, and due to the fact that the end of the hollow bottom frame is of a quarter-circle arc-shaped structure, the shape of the arc-shaped extension piece is matched with the end of the hollow bottom frame; when the unmanned aerial vehicle runs on the water surface, the end of the hollow bottom frame is matched with the arc-shaped extending pieces to form a C-shaped structure, at the moment, the four propeller assemblies are located at the working positions, and the purpose that the unmanned aerial vehicle runs on the water surface can be achieved based on control over the four propeller assemblies.

The application discloses an amphibian radiation dose rate acquisition method and device, and belongs to the field of nuclear radiation protection. The method comprises the following steps: calculating a first radiation dose rate of an amphibian in a first ecosystem and a second radiation dose rate of the amphibian in a second ecosystem; and acquiring a radiation dose rate of the amphibian according to the first radiation dose rate and the second radiation dose rate. The method can solve the problem of low accuracy of the calculation result of the amphibian radiation dose rate in the related art, and has a safety hazard.