16 results about "Amphibian" patented technology

The application discloses a construction method and application of an ovary cell line of Quasipaa spinosa. The method comprises the following steps: selecting metamorphosis period larva ovary tissue, disinfecting and obtaining the ovary tissue, and then using collagenase I digestion solution to digest the ovary tissue at 26 DEG C for 1 hour; after the tissue block adheres to the wall, using a low-osmotic-pressure complete culture medium to carry out primary culture at 25-27 DEG C, wherein the culture medium is 60% diluted DMEM / F12 culture medium containing 10%-15% fetal bovine serum; when the cell confluence degree is higher than 70%, using trypsin-EDTA to digest the cells for 1-2 minutes to subculture, and the subculture culture medium contains 30% cell conditional culture medium; and finally, the cells are cryopreserved through programmed cooling. The ovary epithelial cell line of Quasipaa spinosa is successfully established for the first time, the survival rate of the cells after recovery is high, and the karyotype is stable. The constructed cell line can be used for amphibian reproductive biology research, compound screening and in-vitro conservation of germplasm resources.

The present invention relates to formulations and methods for humanely controlling invasive amphibian populations, and in particular the cane toad, comprising 2-phenoxyethanol and ethanol and / or isopropanol as carrier.

The utility model discloses an amphibian breeding device which comprises a device body, an air cylinder is connected to the back face of the device body, a telescopic rod is slidably connected to the inner wall of the air cylinder, a scraping rod is connected to the end, away from the air cylinder, of the telescopic rod, a sealing frame is connected to the outer surface of the scraping rod, and a sealing plate is hinged to the front face of the device body. The front face of the device body is connected with an impurity box, the front face of the impurity box is hinged to a baffle, and the front face of the baffle is connected with a handle. The air cylinder is arranged to drive the telescopic rod to push impurities out of the device through the scraping rod, the impurities at the bottom are automatically scraped and pushed, the phenomenon that bacteria are generated due to excessive impurities at the bottom can be effectively prevented, and therefore cleanliness and health of water are maintained; the aim of only removing impurities without replacing all water is achieved, water resources are greatly saved, the baffle is opened by pulling the handle for cleaning, the water does not need to be manually drained for cleaning, and the labor intensity is reduced.

A composition for managing, controlling, neutralizing, killing, or repelling an amphibian or reptile comprises at least one active ingredient comprising an effective amount of thymol or a derivative thereof and an effective amount of eugenol or a derivative thereof. The composition is also useful for protecting, guarding, or repelling a pet.

A silt bucket for a road drain, comprising a wall and a bottom area, characterized in that at least one interior area of ​​the silt bucket has one or more integrated exit areas accessible from the interior near the bottom and comprising a climbable structure, wherein the climbable structure is dimensioned and its structural sections are arranged relative to each other in such a way as to enable an amphibian and / or other small animal located in the silt bucket to grasp, support itself and ascend in a directed manner to an upper edge, exit or transition area of ​​the silt bucket, and wherein drainage and / or overflow openings with a free passage cross-section larger than that of the climbable structure are arranged outside the respective exit area.

The invention relates to the technical field of artificial breeding of amphibians, in particular to an ecdysis promoting method for artificially imitating wild breeding of toads and application, according to the ecdysis promoting method, the toads are soaked in a soaking solution containing iodophor, the effective iodine concentration in the soaking solution is 0.1-0.2%, and the soaking time is 10-25 minutes. By the adoption of the ecdysis promoting method, the ecdysis period is greatly shortened, and compared with natural ecdysis (12-15 days), the ecdysis period is shortened to 5-7 days, and efficiency is greatly improved; and the toad cortex quality and yield can be obviously improved, and the toad cortex integrity reaches 92% or above.

The invention discloses a construction method and application of an ovarian cell line of hapalogenys nitens. The method comprises the following steps: selecting a metamorphosis stage larva ovarian tissue, disinfecting and obtaining, and digesting for 1 hour at 26 DEG C by using collagenase I digestive juice; after the tissue block adheres to the wall, a low-osmotic-pressure complete medium is used for primary culture at the temperature of 25-27 DEG C, and the medium is a 60% diluted DMEM / F12 medium containing 10%-15% of fetal calf serum; after the cell confluence degree exceeds 70%, trypsin-EDTA (Ethylene Diamine Tetraacetic Acid) is used for digesting for 1-2 minutes for passage, and a passage culture medium contains 30% of a cell condition culture medium; and finally performing programmed cooling and cryopreservation. According to the method, the ovarian epithelial cell line of the hapalogenys nitens is successfully established for the first time, the cell recovery survival rate is high, and the karyotype is stable. The constructed cell line can be used for amphibian reproductive biology research, compound screening and germplasm resource in-vitro preservation.

The utility model relates to ecological prevention and control technical field especially relates to a multilayer river course wood pile revetment, including water retaining area and revetment area, the revetment area sets up at the outside of river bank, is equipped with the diaphragm between the inside of revetment area and the outside of river bank, water retaining area sets up in the river course of revetment area outside, water retaining area includes first wood pile, water retaining area gravel layer, water retaining area soil layer and submerged plant, the utility model discloses through setting up the double -deck protection of water retaining area and revetment area, has improved the water flow impact resistance of river bank significantly, and water retaining area and revetment area fill gravel, soil and plant and plant, provide the continuous ecological corridor for fish spawning, benthic animal habitat, amphibian migration and bird foraging.

The present application relates to a kind of abdominal spot frog antibacterial peptide Nv-CATH and its gene and application, the antibacterial peptide Nv-CATH is the circular polypeptide coded by the defense peptide gene of amphibian species unique species abdominal spot frog in China, molecular weight 3356.98 Dalton, isoelectric point 11.56, its amino acid sequence is as shown in SEQ ID NO:1.The gene (GenBank accession No.OP264074) of the precursor of the abdominal spot frog antibacterial peptide Nv-CATH is composed of 714 nucleotide sequences, its nucleotide sequence is as shown in SEQ ID NO:2, wherein the 373-522th nucleotide is the encoding gene of mature abdominal spot frog antibacterial peptide Nv-CATH.The application of abdominal spot frog antibacterial peptide Nv-CATH in preparing the therapeutic drug of the infection disease caused by hemolytic staphylococcus, enterococcus faecalis, staphylococcus aureus, klebsiella pneumoniae, acinetobacter junni and / or paratyphi A.The present application provides a new antibacterial peptide Nv-CATH with strong broad-spectrum antibacterial activity.

Small animal guidance system for ground-dwelling small animals, comprising a traffic route VW (2) of a two-way ramp culvert ZRD (7) crossing under it, which extends along a passage for receiving and guiding small animals migrating towards it, in particular amphibians, characterized in that on at least one side of the traffic route (2) in the area in front of the culvert (7) a demarcated, bundling and guiding bundling quarter BQ (4) is arranged and formed on a main crossing axis HQA (3), which defines a central path oriented towards the culvert ZRD (7) at a distance (A) between the bundling quarter (4) and the culvert ZRD (7).

An animal proof fence (1) comprising one or several panel elements (2) which are provided to extend upright to form a barrier, wherein the fence (l) comprises an upper zone (A) wherein at the height of the upper zone (A) the one or several panel elements (2) comprise a smooth unperforated surface on which reptiles, such as snakes, amphibians and small mammals cannot climb and a lower zone (B) wherein at the height of the lower zone (B) the one or several panel elements (2) comprise perforations allowing view through the fence (1).

A lure is disclosed. The lure comprises a head, a tail, a first channel extending inside the head to the tail, a pair of hind legs extending from the tail and including a first leg having a first foot, a second leg having a second foot, the first foot being connected to the second foot forming a foot body having a second channel. The lure has a line having a first end and a second end, and the second end is provided through the first channel and the second channel. The second end is connected to the foot body. The lure may resemble a frog, insect, or amphibian.

The utility model discloses an assembly type spiral channel bank protection structure, which belongs to the field of bank protection structures, and comprises a plurality of protection components, each protection component comprises a dike block, one side of the dike block is provided with a first bulge, the other side of the dike block is provided with a first groove, the first bulge is matched with the first groove of the dike block of the adjacent protection component, and the first bulge is matched with the first groove of the dike block of the adjacent protection component. The first grooves are matched with the first protrusions of the embankment blocks of the adjacent protection assemblies. The fastening strips are connected with the embankment block, and a gap is formed between every two adjacent fastening strips; and the spiral channel body is propped between the embankment block and the plurality of fastening strips and is communicated with the gap. The utility model has the technical effects of not only being capable of adapting to water level change to provide inhabiting space for aquatic animals, but also being capable of serving as a buffer channel for land and water to provide a channel for amphibians.

The application discloses a kind of triflufensulfuron-methyl metabolite prediction and preparation method, it is related to pesticide detection technical field, including M-1, M-2 and M-3;Wherein the structural formula of M-1: the structural formula of M-2: the structural formula of M-3: the application further provides the preparation method of triflufensulfuron-methyl main metabolite M-1, M-2 and M-3, the determination of its synthetic method is conducive to further carry out triflufensulfuron-methyl main metabolite in the adsorption / desorption of environment, leaching, migration and conversion environmental behavior research and to aquatic organisms (zebrafish, black shell shrimp), terrestrial animals (red aposhechens earthworm) and amphibian (tadpole) acute toxicity and chronic toxicity mechanism research.

The application discloses an amphibian radiation dose rate acquisition method and device, and belongs to the field of nuclear radiation protection. The method comprises the following steps: calculating a first radiation dose rate of an amphibian in a first ecosystem and a second radiation dose rate of the amphibian in a second ecosystem; and acquiring a radiation dose rate of the amphibian according to the first radiation dose rate and the second radiation dose rate. The method can solve the problem of low accuracy of the calculation result of the amphibian radiation dose rate in the related art, and has a safety hazard.