The invention designs a semi-closed floating type multi-layer ecological culture water tank. The water tank comprises a water tank floating ring, an upper-layer filtering water permeable ring, a watertank upper-edge water partition ring, an upper-layer grating plate, a sealed water tank vertical wall, a middle-layer grating plate, a bottom-layer grating plate, a conical water tank bottom, a sewage discharge filtering cover, a sewage storage tank, an inflation system, a sewage discharge system and a bait feeding system. By using the water tank, planktonic feeding fishes are stocked in an upper-layer culture area, feed eating fishes are stocked in a middle-layer culture area, and bottom fishes are stocked in a bottom-layer culture area. An inflation system is used for conducting inflation and oxygen enrichment on a water area of the water tank, a bait feeding system is used for feeding bait, residual bait enters the bottom-layer culture area to be eaten by the bottom fishes, fish manureand other pollutants are accumulated in the sewage storage tank, and the accumulated pollutants are discharged out through a sewage discharge pump. At the same time, the upper-layer filtering water permeable ring on the upper portion of the water tank body filters surrounding water when the pollutants are discharged, the water then enters the water tank body, only a small amount of water needs tobe supplemented, the culture waste pollutants are extracted from the water area for discharging, the surrounding environment of the culture areas is not influenced, and the requirements for ecological and environmental protection are met.