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Preparation method of artificial seeds of submerged plants (hydrilla)

A technology of artificial seeds and submerged plants, applied in botany equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of high experimental conditions and operation requirements of plant tissue culture technology, cumbersome preparation methods, and limited application value. Achieve the effect of eliminating plant tissue culture, reducing the preparation process, and strong applicability

Inactive Publication Date: 2014-01-01
SOUTH CHINA NORMAL UNIVERSITY +1
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Problems solved by technology

However, this method first induces sprouting on a hormone-containing solid medium, that is, induces sprouting through a plant tissue culture method. The experimental conditions and operation requirements of plant tissue culture technology are high, and the preparation method is loaded down with trivial details, high risk, long period, and Application value is subject to certain restrictions

Method used

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  • Preparation method of artificial seeds of submerged plants (hydrilla)
  • Preparation method of artificial seeds of submerged plants (hydrilla)
  • Preparation method of artificial seeds of submerged plants (hydrilla)

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Embodiment

[0022] The artificial seed preparation method of the submerged plant black algae of the present invention comprises the following steps:

[0023] 1) Take the black algae seedlings and wash them with water to remove the plankton on the plant surface;

[0024] 2) Cut off all the roots and leaves of the plant, take the vigorously growing plant, and rinse it with distilled water;

[0025] 3) Cut off the lateral buds and terminal buds with internodes, and place them in a gel solution mixed with sodium alginate + Hoagland's nutrient solution + naphthaleneacetic acid (NAA);

[0026] 4) Use a dropper to drop the gel solution containing black algae buds into CaCl 2 Carry out the curing reaction in the middle, take it out after the curing reaction, rinse with sterile distilled water, and terminate the reaction to obtain the artificial seeds of black algae.

[0027] The above-mentioned gel solution is a gel solution formed by mixing 1.5% sodium alginate + 50% Hoagland's nutrient solut...

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Abstract

The invention relates to a preparation method of artificial seeds of submerged plants (hydrilla). The method comprises the following steps: 1. washing the taken hydrilla seedling with water to remove plankton on the surface of the hydrilla seedling; 2. cutting off the roots and leaves of all the plants, picking up the eugenic plants and washing the eugenic plants with distilled water; 3. cutting off the lateral buds and the terminal buds with internodes and putting the lateral buds and the terminal buds into gel liquid which is formed by mixing of sodium alginate, hoagland's nutrient solution and NAA (nicotinic acid amide); 4. dropping the gel liquid containing hydrilla buds into CaCl2 by a dropper to perform curing reaction, taking out gel liquid after the curing reaction and rinsing with sterile distilled water, and terminating the reaction to obtain the artificial hydrilla seeds. According to the method, the step of plant tissue culture is eliminated, and the method is not required to be implemented under an aseptic condition, the preparation process is greatly reduced, the cost is greatly reduced, the period is shortened, and the survival rate is high.

Description

technical field [0001] The present invention relates to a method for propagating submerged plants, which is a method of applying artificial seed technology to the propagation of submerged plants, in particular to a method for making artificial seeds of submerged plants Hydrilla, which belongs to the artificial seeds of submerged plants Hydrilla Innovative techniques for production methods. Background technique [0002] Artificial seed technology is to embed somatic embryos or meristems (buds, callus, embryoids, etc.) Granules formed that can germinate and emerge under suitable conditions. In 1981, after Lawrence et al. studied the lyoglue embedding tape technology wrapped by cells of celery and celery baskets, there were more and more reports on artificial seeds. In 1987, my country began to incorporate artificial seeds into the national high-tech development plan (863 plan), and studied the artificial seeds of some economic crops such as rubber trees and some endemic frui...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 闫志强刘黾陈章和刘燕吴小业刘俊
Owner SOUTH CHINA NORMAL UNIVERSITY
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