385results about How to "Low survival rate" patented technology

Compositions, methods and kits are described for identifying biomolecules (e.g., proteins and nucleic acids) expressed in a biological sample that are associated with the presence, development, or progression of a disease (such as cancer), or more generally determination of the etiology or risk factors associated with a disease. Sample types analyzed by the disclosed methods include but are not limited to archival tissue blocks that have been preserved in a fixative, tissue biopsy samples, tissue microarrays, and so forth. The methods disclosed herein correlate expression profiles of biomolecules with various disease types, and allow for the determination of relative survival rates; in some embodiments, the methods permit determination of survival rates for a subject with cancer. In other embodiments, the disclosure relates to methods for evaluating therapeutic regimes for the treatment, such as treatment of cancer.

The invention relates to a seed coating agent and coated seeds for desert control and aerial seeding, and a preparation method of the agent. The seeding coating agent mainly comprises an inner layer coating agent, a middle layer coating agent and an outer layer coating agent. As the coating method provided by the invention adopts a layered coating technology, not only is the mutual influence among different ingredients of the coating agent alleviated, but also the difficulty caused by the swelling and the adhesion of a water retaining agent which absorbs water in a coating process is avoided; special drying equipment is not needed after the coating is finished, so that the dependence of a traditional coating operation on the drying equipment is avoided; a repellent for birds, rabbits, mice and the like is added to the coating agent, so that the seeds are protected from being eaten while no harm is caused to the environment; and the coated seeds have a high germination rate and can take root rapidly under the action of the water retaining agent and nutrients, so that the seeds for desert control and aerial seeding are promoted to germinate and grow rapidly, and the survival rate of seedlings is remarkably improved.

A method detects a biological condition and detects a change in the biological condition of a mammal. The device determines if the change in the biological condition exceeds a predetermined threshold and sends a signal based at least in part on the determination.

The invention discloses a preparing and purifying method of dealing modularization air-float artificial mattress of wastewater, wherein the main frame of bed display member frame structure as cube box; the bottom, angle and side wall connected with pipes; the top open up to the air; the grid plate is set on the bottom of the bed; the planting box is set on the grid plate; planting vegetation through the planting hole; setting several floats on the side wall; counteracting the weigh of bed body with floatage; keeping balance of bed body in the water; installing ports for passing water on the bottom of float bowl with at least 2 float bowl on the holder; inserting gas-filled lamp into float bowl through ports for passing water; equipping inflating gate valve on the other side of gas-filled lamp. The invention possesses rational structure and simply production, which can improve the survival rate of water plant.

The invention relates to a method for hardening and transplanting savatier monochasma herb. A savatier monochasma herb tissue culture rooting seedling is taken as a test material, and research is carried out from the aspects of a rooting bottle seedling domestication transition method, shading and moisturizing treatment, transplanting matrix screening, matrix ratio, water and fertilizer retentionmanagement and the like. The technical problems of low survival rate and poor transplanting growth adaptability of the savatier monochasma herb rooting seedling in transition in the seedling stage e transplanting into a container are solved, an appropriate seedling hardening technology is provided for the heliophilous savatier monochasma herb rooting seedlings, the savatier monochasma herb rootingseedling smoothly gets through the seedling stage, and a good growth environment is further provided for the transplanted savatier monochasma herb seedling by selecting a high-quality cultivation container, a cultivation medium and a water and fertilizer management mode.

The invention discloses a culture method of Tilapia. The culture method comprises the following steps of: selecting a pond which is 2 meters deep, and disinfecting the pond with unslaked lime after emptying the pond in late October every year; before late November, putting grass carp fries which are all heavier than 250g and feeding with a floating grass carp granulated feed when the water temperature is higher than 15 DEG C; in the next year, putting overwintering Tilapia fries which are all heavier than 200g when the water temperature of the pond is higher than 18 DEG C steadily, and feeding the Tilapia fries twice every day; in late July, fishing 90% of Tilapias for sale, then putting current Tilapia fries which are all heavier than 200g, feeding with a Tilapia floating feed containing 28% of protein in a feed ratio of 1-3%; and fishing all the fishes for sale in late October. By utilizing the culture method, the survival rates of Tilapia and grass carp are higher than 99% and 98% respectively, the yearly output of the pond is improved by 15%, and the commercial fishes are large and uniform in specification and are good in quality. The culture method is a healthy ecological culture mode mainly for culturing Tilapia.

The invention relates to a seedling scion grafting method, which comprises the following steps of (1) sun-shading greenhouse, cutting seedling bed and cutting substrate preparation; (2) scion and stock preparation: new tips of a well-bred parent tree under the healthy growth condition are selected as scions, new tips of a well-bred parent tree under the strong growth condition are selected as stocks, the length of the stocks is about 2 to 10cm, 1 to 2 sections of leaves are left, and the left leaves are positioned in positions being 1 to 2cm below an upper shearing opening and positions being 1 to 4cm above a lower shearing opening; (3) stock pretreatment; (4) grafting: a cleft grafting method is adopted, the stock is flatly placed to be tightly adhered to a base plate, and a split opening with the length being 0.5 to 1cm is longitudinally cut in the position of the upper shearing opening, the scions with the length being 2 to 3cm is sheared, 1 to 2 buds are remained, a two-sided wedge shape with the sharp top end and the length being 0.8 to 1.2cm is cut in a position being 1cm below a scion leaf, the cut scions are aligned with the split opening of the stock, in addition, at least one side is required to form layer aligning, the cut scions are slightly inserted so that the cut scions and the stock are tightly pasted, the middle position of an aluminum sheet covers a grafting opening, and the aluminum sheet is tightly pinched and folded back so that the fixation is realized; (5) cottage; and (6) daily management.

The invention discloses floating full-ripened pellet feed for marine fishes, and a preparation method thereof. The floating all-ripened pellet feed has the density of not greater than 1 and the ripened degree of 100 percent. The preparation method comprises: taking brown fishmeal, blood globulin powder, cottonseed kernel protein powder, flour, wheat, non-transgenic soybean meal, rapeseed meal, cassava pieces, mineral premix, vitamin premix, monocalcium phosphate, fish oil, calcium propionate, oligosaccharide as raw materials; proportioning the raw materials according to part by weight to form feed base material; using double-shaft extrusion full-ripening expansion machine to adjust quality under the steam pressure between 0.4 and 0.5 MPa, at the humidity between 19 and 23 percent and at the temperature between 85 and 90 DEG C; and ripening the obtained product for 90 to 120 seconds, wherein the expansion parameters of an extruder are as follows: a ripening temperature of 180 DEG C, the chamber pressure between 22 and 28 atm and a screw speed of 450 to 550 r/min. The feed has the advantages of low density, full-ripened property, all destroyed anti-nutritional factors, high stability in water and small feed coefficient.

The invention discloses a fermentation process of a sea cucumber compound feed, which comprises the following steps: 1-2g of bacillus subtilis at least with the concentration of 1010CFU/g, 20-50ml of lactic acid bacteria at least with the concentration of 109CFU/ml, 0.5-0.8g of yeast at least with the concentration of 1010CFU/g, 5-10g of glucose and 300-400ml of purified water or sterile water are added to a sea cucumber feed, and then acetic acid is used for regulating the pH value to 6.0-6.8; and fermentation is carried out for 12-24 hours at 20-35DEG C. The process is simple, the operation is easy, the production cost is low, and probiotics in the feed are increased, thus promoting digestion of nutrients that are difficult to digest, improving the palatability of the feed, enabling stichopus japonicus to grow rapidly and to have high survival rate and improving the water quality.

The invention discloses a method for sterile seeding and rapid propagation of bougainvillea speetabilis. The method comprises the steps of conducting sterile seeding and induction germination on seeds, wherein the seeds are sterilized, then a shoot induction medium is inoculated with the sterilized seeds, and shoot induction culture is conducted at proper temperature with proper illumination time and illumination intensity; conducting seedling breeding through bud expanding propagation and rooting reinduction, wherein a sprout enrichment culture medium is inoculated with germinated buds, and bud propagation subculture is conducted at proper temperature with proper illumination time and illumination intensity; inoculating a rooting medium with the bud propagation subcultured bud seedlings, and conducting rooting breeding of seedlings at proper temperature with proper illumination time and illumination intensity. According to the method, seeds are used as breeding explant, seed treatment is easy, pollution rate is low, the germination rate of seeds reaches 100%, bud propagation rate reaches 5.22 times within 30 days, rooting rate is 95.6%, and seedling breeding is not limited by time or season so that whole-year production can be conducted.

The invention provides a pure traditional Chinese medicine composition for treating male infertility. The pure traditional Chinese medicine composition is made from the following materials in parts by weight: 3-6 parts of aconite accessory tuber, 3-6 parts of cassia, 8-10 parts of fried Chinese yam, 12-20 parts of prepared rehmannia root, 3-6 parts of schisandra fruit, 8-10 parts of fruit of Japanese metaplexis, 5-10 parts of morinda root, 5-10 parts of fructus psraleae, 12-20 parts of calcined dragons bone, 12-20 parts of calcined oyster, 8-10 parts of lotus seed, 12-20 parts of epimeddium, 3-6 parts of alisma rhizome, 3-6 parts of poria, 8-10 parts of pubescent pepper herb, 6-10 parts of fried eucommia bark, 3-6 parts of tree peony bark, 5-10 parts of raspberry, 8-10 parts of gordon euryale seed, 6-10 parts of rhizoma curculiginis, 8-10 parts of milkuetch seeds, 8-10 parts of Chinese chive seed, 8-10 parts of Chinese dodder, 8-10 parts of cherokee rose-hip, 15-25 parts of calcined actinolite, 8-10 parts of glossy privet fruit, 8-10 parts of broomrape, 6-10 parts of common fenugreek seed, 6-10 parts of cynomorium and 6-10 parts of yam. The invention further provides a pure traditional Chinese medicine preparation prepared thereby. The pure traditional Chinese medicine composition is based on strengthening yang and tonifying kidney, aims at reinforcing sperm motility and can effectively treat male infertility and relevant diseases. The pure traditional Chinese medicine preparation has the advantages of simple preparation, low production cost and convenient administration.

The invention provides a sexual propagation method of bletilla striata. The sexual propagation method of bletilla striata particularly comprises the steps of processing of capsules, preparation of nutrients, inoculation and cultivation, and bottle out domestication, and is characterized in that in the step of processing of capsules, an alcohol lamp drying mode replaces a filter paper sucking dry mode in the prior art; in the step of preparation of nutrients, combination of banana slurry and humus is adopted; in the step of inoculation and cultivation, only inoculation, germination cultivation and strong seedling cultivation steps are included, the conventional enlargement cultivation process in an existing tissue culturing technology is omitted, and the process is simplified and the cost is lowered while the purposes are achieved; in the step of bottle out domestication, cultivation is performed through humus in a greenhouse, domestication is finished under the condition of controlling the relative humidity, temperature and shading rate, and qualified bletilla striata seedlings are obtained. Bletilla striata seeds are used for scale seedling culturing, the survival rate is high, manual scale propagation of bletilla striata is achieved, and the obtained bletilla striata seedlings are low in contamination rate, high in differentiation rate and stable in inheritable character.

The invention discloses a method of potato virus eradication, which includes the following steps: taking a material for virus detection, carrying out the tissue cultivation to determined material with virus, and then establishing a simple-bud material; carrying out reproducing successive-transfer cultivation to the established simple-bud material and ultra-low temperature storage treatment to the simple-bud material, reproducing the survived material after ultra-low temperature storage, and then carrying out virus detection to the survived material; and reproducing the material without virus after detection to be used for producing seed potato without virus. Compared with the traditional stem-tip virus eradication, the method of the invention has lower operation difficulty and higher virus eradication rate, and the virus which can not be easily eradicated by stem-tip cultivation can be eradicated by the method. The invention has excellent social and economic values and wide application prospect.

The invention discloses a modified top grafting method for bud grafting of cortex jatrophae and belongs to the technology field of plant grafting. The method comprises the steps of selection of bud grafting time and scionwood, bud grafting operation and post-bud grafting management. The bud grafting is performed during the second third of February to the last third of March or the first third of September to October. A grafting bud of 2.0-3.0cm length and 0.8-1.2cm width is cut from a lignified or semi-lignified twig of good-quality cortex jatrophae by using a grafting knife. A graft interface is formed on a root stock and has the same size as the bud piece. The interface should be cut through the cortex and deep into the xylem. The bud piece is grafted after saps of the grafting bud and the root stock are drained, about 10 minutes after root stock cutting. The grafting bud is inserted into the interface and sealed by binding, followed by timely examination and graft supplementation. After the graft survives, the bondage is removed, the stock bud is erased, and the stock pile above the interface is sheared off, thereby promoting the survival. The method has the advantages of short time, rapid action and high survival rate of grafted bud (above 96%).

An artificial feeding method at low altitude for a host insect ghost moth of Ophiocordyceps sinensis. This method mainly includes: selecting an appropriate disinfectant for performing surface disinfection on ghost moth eggs, and then placing the disinfected eggs in sterile humus; feeding ghost moth larvae with carrots, disinfecting and detecting the fed carrots, and culturing the larvae till male and female pupae are obtained; identifying male and female pupae of the ghost moths, placing them at different culture temperatures, wherein the culture temperature for the male pupae is 2-6° C. lower than that of the female pupae, resulting synchronously emerged male and female adults; placing the emerged male and female adults in an adult cage with simulated plants for mating and staying of the adults, maintaining a humidity over 80%, and employing 50-100 Lux shimmer for facilitating activity and mating of the adults, thus improving fertilization opportunity and hatchability of eggs when the adults after mating lay eggs.

The invention mainly discloses a constant-temperature silkworm frame applied to bombyx mori cocooning. The constant-temperature silkworm frame comprises a bottom frame, a silkworm breeding frame, a feeding frame, a ventilating frame and a top board which are arranged from bottom to top. A first heating device is disposed in the silkworm breeding frame. The first heating device comprises a thermal insulation board, a heating plate, a warming plate and a temperature-sensitive plate. Side portions of the feeding frame are provided with feeding holes, and a feeding plate is disposed in each feeding hole. Front and back sides of the ventilating frame are uniformly provided with air vents. An upper portion of the right side of the ventilating frame is provided with a slot. The top board is inserted into the slot to cover the ventilating frame. The top board is provided with a second heating device and a power supply device. The second heating device comprises a heating lamp, a temperature-sensitive rod, a display screen and an adjusting button. The heating lamp is installed at a lower surface of the top board. The temperature-sensitive rod is movably connected to the top board via a movable plate. Temperature of a silkworm breeding environment is strictly controlled, a good survival environment is provided for silkworms, survival rates of the silkworms are improved, feeding is convenient, permeability is good, cultivation space is large, breeding efficiency is high, and the constant-temperature silkworm frame is suitable for large-scale silkworm breeding.

The present invention provides, among other things, therapeutic compositions and methods that can effectively treat, slow or prevent mild cognitive impairment (MCI) or Alzheimer's disease (AD), in particular, based on therapeutically effective amount of nifedipine, oxidized or nitroso nifedipine derivatives, thyroxine (T4), triiodothyronine (T3) and combinations thereof.

The invention discloses a method for cutting lonicera similis hemsl, which comprises steps of seedling bed preparation, branch selection, the cutoff of cutting wood, clear water treatment of the cutting wood, willow branch water treatment of the cutting wood, cutting, seedling bed management and the like. The method performs breakthrough improvement on the prior cutting method, can ensure that the survival rate of the lonicera similis hemsl is remarkably improved by performing the clear water treatment on the cutting wood before the cutting, ensure that the survival rate of the cutting can reach over 60 percent, has good growth, improves the survival rate by over 50 percent than the prior cutting method, and has extremely remarkable effect; and the survival rate of the cutting wood can also be further improved if the cutting wood is subjected to the willow branch water treatment before the cutting. In addition, the method also has the characteristics of simple and easy operation, lower cost and the like, and is easy for popularization.

The application discloses a plant watering reminding method, device, equipment and storage medium, and it belongs to the field of image data processing. The method includes: acquiring a target video,wherein the target video includes an appearance image of the plant; extracting a part image corresponding to different parts of the plant from the target video, the part image comprises at least one of a leaf part image, a stem image and a root image; inputting a part image into a water content prediction model to obtain the water content of the plant, water content prediction model is obtained from the sample part image and sample water content date training; performing watering reminding according to the water content; In the embodiment of the application, since the water content predictionmodel is obtained from training a large number of sample part images and sample water content data, as a result, the predicted water content can truly reflect the current water shortage state of the plant, so that the user can irrigate the plants at a suitable time according to the watering reminding.