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Paper mulberry seedling tissue culture method suitable for extensive plantation

A tissue culture, large-scale technology, applied in the field of plant propagation, can solve the problems of unused ecological and economic benefits, and achieve the effect of strong practical significance, speed up and easy operation

Inactive Publication Date: 2012-05-23
HUBEI GOLE BIO TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, scholars at home and abroad focus on the papermaking raw materials and pharmacological components, etc., which are far from giving full play to their ecological and economic benefits. The introduction, breeding and application research of excellent provenances of mulberry are urgently needed. Strengthen scientific research and development

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Take the healthy and plump germ material at the top of the mulberry tree, use cotton balls to add a small amount of detergent to the water to gently wipe the branches and their buds, rinse with tap water for 1.5 hours, and use 70% alcohol on an ultra-clean workbench Sterilize for 30 seconds, rinse with sterile water for 3 times, sterilize with 0.1% mercuric chloride for 3 minutes, and rinse with sterile water for 3 times. The surface-sterilized materials were inoculated on the blank DCR germination medium and placed in a light incubator for cultivation. In the experiment, 50 apical germs were taken respectively, and 3 groups were set up to repeat the experiment. They were cultured for 3 weeks at a temperature of 25 (±2)°C, a light time of 12h / d, and a light intensity of 1500lx, and the germination rate was counted based on the growth of young leaves. . Then take 50 germinated seedlings with a seedling height of 1.0 to 3.0 cm, cut them into leafy stem segments of 5 to 1...

Embodiment 2

[0020] Take the healthy and plump germ material at the top of the mulberry tree, use cotton balls to add a small amount of detergent to the water to gently wipe the branches and their buds, rinse with tap water for 1.5 hours, and use 70% alcohol on an ultra-clean workbench Sterilize for 30 seconds, rinse with sterile water for 3 times, sterilize with 0.1% mercuric chloride for 3 minutes, and rinse with sterile water for 3 times. The surface-sterilized materials were inoculated on the blank DCR germination medium and placed in a light incubator for cultivation. In the experiment, 50 apical embryos were taken respectively, and 3 groups were set up to repeat the experiment. They were cultured for 3 weeks under the conditions of temperature 25 (±2) ℃, light time 14h / d, light intensity 1800lx, and the germination rate was counted based on the growth of young leaves. . Then take 50 germinated seedlings with a seedling height of 1.0 to 3.0 cm, cut them into leafy stem segments of 5 ...

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Abstract

Belonging to the field of plant propagation, the invention discloses a paper mulberry seedling tissue culture method suitable for extensive plantation. After top plumules are grown into buds on germination medium, the buds are inoculated onto bud induction medium for propagation, axillary buds and adventitious buds are grown out, so that rootless seedlings are obtained, and the healthy rootless seedlings are then transferred onto rooting medium for adventitious root induction. The components of the mediums are as follows: germination medium: DCR; bud induction medium: 6-BA and NAA are added to the germination medium; and root induction medium: 6-benzyladenine (6-BA), Alpha-naphthylacetic acid (NAA) and indolebutyric acid (IBA) are added to the germination medium. After induced propagation, the majority of adventitious bud leaves are thick, stems are bulky, and after multiple times of subculture, the buds grow normally. The paper mulberry seedling tissue culture method has the advantages of low mutation rate and high propagation coefficient, the survival rate of acclimatized seedlings reaches more than 90 percent, and the paper mulberry seedling tissue culture method is suitable for providing seedlings for the extensive plantation of paper mulberries, and can bring good economic and social benefits.

Description

technical field [0001] The invention mainly relates to a tissue culture method for mulberry tree seedlings suitable for large-scale planting, and belongs to the field of plant propagation. Background technique [0002] The mulberry tree is a unique fiber-containing tree species in my country, and it is a special economic forest. Its bast fiber and leaves have extremely high utilization value, and the market development prospect is broad. It is widely distributed, highly adaptable, easy to reproduce, resistant to drought and barren, and is a good ecological and fuelwood tree species. The protein content in the leaves of the mulberry tree is very high, up to 20-30%, much higher than that of rice, corn, and wheat, and second only to soybeans; in addition, the content of amino acids, vitamins, carbohydrates, and trace elements is very rich. The pure natural raw material of the feed, the pigs fed with this feed have high lean meat rate, pure meat quality and delicious taste, whi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 张云峰陈晓炜谢明利胡伶俐
Owner HUBEI GOLE BIO TECH DEV
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