178 results about "6-benzyladenine" patented technology

Soluble and stable liquid compositions containing a plant growth regulator selected from the group consisting of cytokinin and a gibberellin, an acid solubilizer such as citric acid, tartaric acid or glycolic acid and a solvent; as well as methods for making and using the composition are disclosed. The compositions improve solubility, handling, stability, safety, as well as activity improvements such as improved plant growth, yield, fruit thinning or sizing and quality. The compositions are soluble and stable by adding an ethoxylated alkyl alcohol wherein the growth regulator is 6-benzyladenine (6-BA) or forchlorfenuron (CPPU) and the ethoxylated alcohol surfactant is C12-15 alkyl alcohol in propylene glycol. The compositions may also contain a cytokinin such as 6-benzyladenine (6-BA) or forchlorfenuron (CPPU) that is increased in solubility and activity and by synergistically combined with GA₃ or GA₄A₇ as well as in storage stability by adding an antioxidant. The compositions are formulated in a ready-to-mix formulation.

The invention relates to low VOC plant growth regulator compositions comprising less than about 25% of volatile organic compounds (VOCs). The compositions preferably comprise 6-benzyladenine (6-BA) or forchlorfenuron (CPPU). Also provided are methods of using the compositions.

The invention discloses a method for producing anoectochilus formosanus by tissue culture, comprising the following steps: frond selection and asepsis, cluster bud inducing culture and large-scale culture. The key to the technique is characterized in that two different culture media are applied to the inducing culture and the large-scale culture, wherein the inducing culture medium is as follows: H culture medium+ 10-50g/L of cane sugar + 0.1-3.0mg/L of alpha-naphthylacetic acid+ 0.1-5.0mg/L of 6-benzyladenine, and pH value is 5.0-7.0; the large-scale culture medium is as follows: B5 culture medium+ 20-40g/L of cane sugar+ 6-8g/L of agar+ 2.0-3.0g/L of active carbon+ 1.0-2.0mg/L of alpha-naphthylacetic acid+ 2.0-3.0mg/L of benzyladenine, and pH value is 5.5-6.5. The method is easy to operate, features low production cost, high output, high quality and no pollution to the environment and can realize batch production.

The invention discloses a mixed rooting agent suitable for various plants. The mixed rooting agent is prepared from the following components in parts by weight: 18-23 parts of triacontanol, 90-108 parts ofboric acid (HBO3), 170-230 parts of naphthalene acetic acid(NAA), 18-23 parts of 6-benzyladenine(6-BA), 750-860 parts ofindole-3-butytric acid(IBA), 17-25 parts ofmanganese sulfate(MnSO4), 17-25 parts ofmagnesium sulfate (MgSO4), 7-15 parts of glycine and 8-13 parts of glutamic acid.As containing the amino acid components, the mixed rooting agent has the effects of providing nutrients and performing physiological regulation, is capable of improving the adversity ability of crops, and is beneficial to crop growth. With a growth hormone formula, the vigor of root systems can be improved, the growth of root systems and fibril can be promoted, and the rate of rooting of cuttings can be enhanced. By virtue of the mixed rooting agent, the content of chlorophyll of transplanted plants can be increased, and the photosynthesis of plants can be strengthened. By virtue of synergism of various hormones and amino acids, the growth of plants is promoted greatly, root hair of plants can be protected effectively, the adaptation of transplanted plants can be prolonged, the rooting speed of transplanted plants can be increased, and the growth of plants in the early state of transplanting can be enhanced.

The invention provides a flower preservative and a preparation method thereof.The flower preservative is prepared from, by weight, 2-8 parts of zinc gluconate, 2-6 parts of iron gluconate, 3-5 parts of sucrose, 1-5 parts of borax, 2-4 parts of aluminium potassium sulfate, 3-6 parts of aluminum ammonium sulfate, 1-5 parts of chitosan, 2-5 parts of chitin, 3-6 parts of magnesium sulfate, 1-4 parts of traditional Chinese medicine extracts, 2-5 parts of 6-benzyladenine, 1-4 parts of forchlorfenuron, 2-4 parts of gibberellins, 1-5 parts of 2,4-dichlorphenoxyacetic acid, 2-6 parts of salicylic acid, 3-8 parts of 8-hydroxyquinoline citrate, 1-6 parts of inositol hexaphosphate, 1-5 parts of ethylene glycol and 8-12 parts of deionized water.The flower preservative is simple to prepare and excellent in preservation effect.

The invention relates to a method for promoting paeonia suffruticosa seedling growth and application thereof. Specifically, the invention provides a paeonia suffruticosa seedling treatment fluid, which comprises: a paeonia suffruticosa seedling growth active agent (gibberellin and/or 6-benzyladenine) and a surfactant, and also can include an agronomically acceptable soluble calcium salt. The growth active agent can break epicotyl dormancy of paeonia suffruticosa seeds, improve the germination rate/germination potential of paeonia suffruticosa seeds, accelerate the paeonia suffruticosa seed growth process, and promote seedling emergency or flowering of paeonia suffruticosa seed seedlings/tissue culture seedlings. The surfactant can make active substances fully enter cells. And the soluble calcium salt is closely related to multiple physiological functions of cells.

The invention discloses a tissue culture method for lilium tenuifolium, which comprises: sterilizing stripped lilium tenuifolium bulb serving as an explant from an intermediate scale part; and inoculating the stripped lilium tenuifolium bulb into a Murashige and Skoog (MS) medium, a first-generation induction medium consisting of 6-benzyladenine (BA) at a concentration of 1.0mg/L, nicotinic acid amide (NAA) at a concentration of 0.1mg/L and vitamin C (Vc) at a concentration of 5mg/L, a sub-generation propagation medium consisting of MS, 6-BA at a concentration of 0.2mg/L and NAA at a concentration of 0.01mg/L, a rooting medium consisting of 1/2of MS, and indole-3-butyric acid (IBA) at a concentration of 0.5 mg/L and bulbing culture medium consisting of 1/2of MS, IBA at a concentration of 0.5 mg/L and 2 percent of white sugar in turn for culture under the culture conditions including a culture temperature of 25+/-1 DEG C, day light and 4 hours of night light supply per day. When the culture method is used, tissue culture lilium tenuifolium seedlings propagated indoor in large scale can be obtained in a short time period, the productivity is high and the potential ecological benefitand social benefit can be created.

The invention discloses a sweet potato distant hybridization breeding method with a high success rate, and relates to the technical field of plant innovation and new variety breeding of crops. The method comprises the following steps of: taking a wide-compatibility parental sweet potato Xushu 18 as a female parent, and regulating the flower season of the Xushu 18 to be synchronous with that of a wild specie sweet potato by a grafting and dark treatment method; performing water culture on a male parent and the female parent indoor to naturally take root and blossom; after the male parent and the female parent blossom, performing hybrid pollination; after pollinating, coating a hybridization treating fluid (40mg/L 6-benzyladenine, 45mg/L 2,4-dichlorphenoxyacetic acid and 40mg/L naphthylacetic acid) on the stem base of an ovary and the whole flower stalk, and continuously treating for 7 days; and for a combined hybridized ovule which stops early development, adopting immature embryo rescue technology. The hybridization method and a hybridization system of the invention have the advantages of high operability and high success rate. The method is also suitable for indoor directed hybridization of a field naturally blossoming sweet potato material and a conventional sexual hybridization breeding of the sweet potato.

The invention discloses a soilless culture method for high-sugar Hami melons, which comprises the steps of sowing and seedling raising, planting, preparing nutrient solution, controlling temperature, pruning and leaving fruit, and the like, wherein the nutrient solution contains 20-50mg/kg gibberellin, 20-50g/kg sodium borate, 3-15g/kg monopotassium phosphate, 10-15mg/kg 6-benzyladenine, 30-50mg/kg brassin and 20-50mg/kg diethyl aminoethyl hexanoate; at different stages, the temperature control is as follows: the temperature is controlled at 18-22 DEG C at night and 25-30 DEG C in the daytime during the period from planting to surviving; the temperature is controlled at 15-20 DEG C at night and 28-35 DEG C in the daytime during the period from surviving to blooming; during a melon growing period, the temperature is controlled at 22-27 DEG C in the daytime and 15-18 DEG C at night, and an ultraviolet lamp is used for illuminating for 1-2 hours with the luminance being 1500-2000muW/cm<2>; and after the melon growing, the shed temperature is kept at 30-35 DEG C in the daytime and 15-18 DEG C at night, and the ultraviolet lamp is used for illuminating for 2-3 hours, with the luminance being 5-10mW/cm<2>. According to the method provided by the invention, the quality such as the sweetness of the Hami melons is increased, and meanwhile, the fruit cracking phenomenon is reduced.

The invention belongs to the field of rapid propagation and tissue culture method of plant, more particularly relates to a culture medium of Prunus serrulata Lindl. and a method of rapid propagation and tissue culture. The culture medium consists of a large number of elements, micro elements, ferric salt, organic components, sycrose, agar and a plant growth regulating agent, a large number of elements thereof comprise: ammonium nitrate, potassium sulfate, monopotassium phosphate, magnesium sulfate heptahydrate, calcium nitrate tetrahydrate and calcium chloride dehydrate; the ferric salt thereof comprises: ferrous sulfate heptahydrate and dihydrate sodium ethylene diamine tetracetate; the plant growth regulating agent is 6-benzyladenine and has concentration of 0.5-1.0 milligram/liter; other components are the same as MS culture medium. The pH value of the culture medium is 5.6-5.8. The culture medium of rapid propagation and tissue culture and the method of rapid propagation and tissue culture, provided by the invention, are simple, convenient and easily feasible aiming at characteristics of Prunus serrulata Lindl.. The tissue culture seedling is free from malformation, elongated in internode, enhanced in propagation coefficient, short in propagation period, which is about 50 days, good in culture effect and high in survival rate.

The invention discloses a stem latent bud germination promoter and a method for promoting the germination of stem latent buds by using the stem latent bud germination promoter. The stem latent bud germination promoter comprises a water solution containing the following components with concentration: 10-30 mg/L of N6-kinetin (KT), 50-200 mg/L of 6-benzyladenine (6-BA), 300-1000 mg/L of gibberellin (GA3) and 200-400 mg/L of 2-chloroethyl phosphoric acids (ethephon). The stem latent bud germination promoter and the method for promoting the germination of the stem latent buds by using the stem latent bud germination promoter, which are disclosed by the invention, and can be used for overcoming the defects of the prior art and promoting the latent bud germination, realizing the latent bud germination on a designated position, ensuring the germination amount and cultivating the pot cultures with good tree crowns.

The invention discloses a detoxification, tissue culture and rapid propagation method of common turmeric, which comprises the explant selection and treatment, induction culture, the virus identification of plant regeneration, proliferation culture, transplanting and other steps. In the invention, on the basis of the MS culture medium, the plant growth regulators such as 6-benzyladenine, kinetin, Alpha-banai acid, adenine sulphate are added for the induction culture of common turmeric apical meristem and the proliferation and rooting of multiple shoot two key stages. With the adoption of the packaged technology and method provided in the invention for the tissue culture and rapid propagation of common turmeric, the difficult problems of common turmeric such as virus accumulation, declining quality and yield reduction caused by propagation coefficient and technical stability are met. Therefore, the invention can provide a seedling-raising method with short cycle, high rate of reproduction, low-cost for the factory production of common turmeric.

The invention relates to a culture method of oriental lily test tube bulbs. An MS culture medium is cultured under the darken condition with the temperature of 25 DEG C, wherein the MS culture mediumcontains 0.1-10.1 mg/L<-1> of 6-benzyladenine, 0.1-10.0 mg/L<-1> of rhodofix and 60 g/L<-1> of sucrose. Small lily bulbs are directly induced from explants, subculture is carried out twice, and a testtube bulb with the diameter of 4-5 cm and the weight of more than 1.5g is formed after 5 months. Temperature-variable treatment is carried out on the bulb through 6-BA combination for 8-15 days at the temperature of 10-15 DEG C, then low-temperature storage is carried out for 50-70 days at the temperature of 3-5 DEG C, and the dormancy is relieved. The stooling rate is more than 95% after the bulb is planted in fields. The diameter of the bulb diameter reaches 10-12 cm after one growing period. By adjusting and controlling the shape development of the test tube bulb and carrying out 6-BA combination temperature-variable induction, the dormancy is relieved at low temperature, the stooling rate of the oriental lily test tube bulb in the fields is enhanced, and the expansion speed of the bulb is increased by one time; the 3 growing periods of the culture time of detoxicating stock seeds in the part is shortened to 2 growing periods, therefore, the production cost is decreased by nearly 1/3.

The invention relates to a foliage fertilizer for promoting growth of cotton buds and bolls. The foliage fertilizer is an aqueous solution, and prepared from the following active ingredients: a nitrogen fertilizer, monopotassium phosphate, borax, auxin, 6-benzyladenine and gibberellin. The invention also relates to a preparation method of the foliage fertilizer. By adopting foliage spraying of the foliage fertilizer provided by the invention at a cotton bud period and a boll period, reproductive growth of the cotton can be facilitated; budding and flowering are facilitated; dropping of the cotton buds and bolls is reduced. Thus, the cotton yield is improved. The preparation method of the foliage fertilizer provided by the invention is simple and convenient to operate, and mild in technological condition.

A nutrient solution for rejuvenating trees in gardens is a nutrient solution, containing macroelements, trace elements, organic substances, physiologically active substances and exogenous hormones required for the growth of plants, prepared from ammonium nitrate, potassium nitrate, urea, magnesium sulfate heptahydrate, potassium dihydrogen phosphate, calcium nitrate, calcium chloride dihydrate, sucrose, ferrous sulfate heptahydrate, disodium ethylenediaminetetraacetate, zinc sulfate, copper sulfate, manganese sulfate tetrahydrate, sodium chloride, boric acid, vitamin A, vitamin C, vitamin B1,vitamin B6, humic acid, naphthaleneacetic acid, indolebutyric acid, 6-benzyladenine and gibberellin. The nutrient solution for rejuvenating trees in gardens can improve the survival rate of transplanted trees and promote and accelerate the self-recovering ability of nursery plants in order to make the nursery plants reach an expected landscape effect. The nutrient solution has a good rejuvenatingeffect on famous and old trees planted for many years and having poor growth vigor, and can enhance the resistance, break the dormancy, promote the germination of new shoots and new sprouts and delayaging.

The invention discloses a callus culture method of snow lotus herb, comprising the steps of selection and asepsis of plants, induction and culture of callus and large-scale culture. The core technology of the method is selection of the following large-scale culture medium: MS+0-50g/L of cane sugar+0-10g/L of agar+0.1-5.0mg/L of alpha-naphthylacetic acid+0.1-5.0mg/L of 6-benzyladenine. During large-scale culture, an abiotic elicitor, 40-60mu mol/L of SNP+40-60mu mol/L of MJ+40-60mu mol/L of SA is added. The method is easy to operate, and the active ingredient is high in content and low in production cost, does not pollute environment and can reach the level of industrialization. Syringin produced by the culture technology in the invention is invariant, high in content, low in cost and short in cycle and is very competitive in the markets.

The invention relates to a method for preparing a quick-acting synergist for a fertilizer. The method comprises the following steps of: smashing and sieving 0.1 to 35 percent of sodium 2,4-dinitrophenolate, 0.1 to 20 percent of alpha-nafusaku, 0.1 to 20 percent of indole potassium butyrate, 1 to 15 percent of polyglutamic acid, 0 to 5 percent of 6-benzyladenine, 0.1 to 5 percent of 2-methoxy-5-nitrophenol in proportion to obtain powder or granules of 20 to 60 meshes; uniformly mixing the components in different types; adding 0.1 to 5 percent of potassium hydroxide or sodium hydroxide which is easy to dissolve in water; and totally mixing the components once again to obtain a finished product. The quick-acting synergist for the fertilizer fulfills the aims of remarkably promoting growing effects of crops within 1 to 2 days, saving the using amount of the fertilizer by 20 to 25 percent, improving the utilization ratio of the fertilizer by 30 to 35 percent, increasing the yield of the crops by 20 to 25 percent and increasing both production and income of the crops, and brings positive and considerable economic and social benefits.

The invention discloses a method for promoting the sprouting of a clematis glauca seed, which is characterized by comprising the following steps of: (1) respectively preparing 100-300mg/L of gibberellin solution and20-40mg/L of 6-benzyladenine solution by adopting acetone as a solvent; (2) soaking the clematis glauca seed in 0.1 percent of potassium permanganate solution for one hour; soaking the clematis glauca seed by using the gibberellin or 6-benzyladenine solution for one hour and then placing in a refrigerator at 9 DEG C for refrigerating for one month; and (3) placing the refrigerated seed into a thermostat at 25 DEG C for promoting sprouting. The method has obvious function of promoting the sprouting of the seed by utilizing the gibberellin and the 6-benzyladenine, the seed sprouting rates respectively reach 64 percent and 67 percent, and the sprouting characteristics of the seed are improved.

The invention provides a method for trivhosantnes kirilouii manim plantation artificial pollination and relates to the trivhosantnes kirilouii manim plantation technical field. The method comprises the following steps: pollen collection is carried out; pollen explosion is carried out, namely, pollen and a pollen explosion promoter are mixed to form a pollen drug, the temperature is controlled at 25-28 DEG C, irradiation of lamp light with an output of 1-5 watts is carried out until pollen is exploded out, and the pollen explosion promoter comprises, by weight, 1 part of ammonium molybdate, 2 parts of potassium acetate and 5 parts of zinc gluconate; pollination is carried out, namely, dilution dipping pollination is carried out at 8-9 o'clock in the morning on a sunny day, a diluent for dilution comprises, by weight, 1 part of boric acid, 12 parts of bentonite, 0.5 part of 6-benzyladenine, 10 parts of starch and 1000 parts of purified water, and the blending method is as follows: pollen is weighed and placed in a measuring cylinder, cane sugar, boric acid, 6-benzyladenine and bentonite are added, then purified water is blended, stirring is carried out, a pollen suspension is prepared, the pollen suspension is dipped and dipping pollination is carried out. Operation is convenient, pollen explosion time is shortened, and the pollination success rate can be raised greatly which facilitates later growth and fruiting of pistillate flowers.

The invention relates to a method for culturing sugar-free tissue of jewel orchid, which comprises the steps of: inoculating the small-segment tissue of the little sterile jewel orchid to a sugar-free induction culture medium, placing the sugar-free induction culture medium in a culture box with a top opening for carrying out tissue induction and culturing cluster buds, wherein the sugar-free induction culture medium comprises an MS culture medium, a porous inorganic material (4-10g/L), alpha-naphthylacetic acid (0.1-3.0mg/L) and 6-benzyladenine (2-4mg/L), and the pH value of the sugar-free induction culture medium is 5-6; and planting the induced cluster buds into a sugar-free strong stock culture medium, placing the sugar-free strong stock culture medium in the culture box with the top opening for culturing strong stocks, wherein the sugar-free strong stock culture medium comprises a B5 culture medium, a porous inorganic material (2-10g/L), peptone (1-5g/L), Hyponex 1 (0.1-3.0g/L), banana paste (20-150g/L) and active carbon (0.1-3g/L), and the pH value of the sugar-free strong stock culture medium is 5.5-6.0. The invention can improve the root-taking rate and the planting percent of the jewel orchid plant, and reduce the production and tissue culture cost.

A tissue culture method for quickly reproducing the raspberries of Tulameen, summit and Blackbuttee includes such steps as choosing explant; preparing the culture medium for induced differentiation from 6-benzyladenine, naphthylacetic acid, cane sugar, agar and activated carbon besides MS culture medium and the culture medium for induced rooting from naphthylacetic acid, can sugar and agar beside 1/2 MS culture medium; pretreating the explant, disinfecting; inoculating; culturing in optical culture room at 25 +/- 2 deg.C under sunlight for 10-12 hr every day; induced differentiation; induced rooting; and culturing sapling.

The invention relates to a regulator for promoting growth of fruit tree flowers and a preparation method of the regulator, belongs to the technical field of fruit tree cultivation and aims to solve the problems of low yield of orchards in season and vigorously growing saplings, hormone residues on fruits and the like. The regulator for promoting the growth of the fruit tree flowers comprises components in parts by weight as follows: 1.2-1.8 parts of choline chloride, 0.6-0.9 parts of sodium bisulfite, 0.6-0.9 parts of potassium bicarbonate, 3.9-5.5 parts of paclobutrazol, 0.8-1.3 parts of uniconazole, 0.2-0.4 parts of 6-benzyladenine, 13.8-16.8 parts of monopotassium phosphate, 11.3-13.8 parts of zinc sulfate, 15.9-19.4 parts of boric acid, 2.4-3.7 parts of ammonium molybdate and 0.6-0.8 parts of a wetting dispersant. A preparation process of the regulator for promoting the growth of the fruit tree flowers is simple, low in cost and convenient to use, the regulator has remarkable shoot controlling, flower growth promoting and yield increasing effects, the hormone residues on the fruits are reduced effectively, the regulator has better social and economic benefits and has wide application prospect in open-ground cultivation, facility cultivation and potting cultivation of various fruit trees.

The invention discloses cut-flower chrysanthemum antistaling agent and a method for refreshing the cut-flower chrysanthemum by using the same. The antistaling agent contains 6-benzyladenine. The cut-flower chrysanthemum antistaling agent can effectively maintain the quality of cut-flower chrysanthemum, prolong the vase life of the cut-flower chrysanthemum for 2-6 days, delay the aging of the cut-flower chrysanthemum, and simultaneously enable the cut-flower chrysanthemum to have full flower type, long-lasting color, bright color and can improve the overall enjoy quality of the cut-flower chrysanthemum.