Culture method of oriental lily test tube bulbs

A method for cultivating bulbs and a technology for bulbs, which are applied in the field of agricultural biology, can solve the problems of unclear mechanism of dormancy release of test tube bulbs, and achieve the effects of reducing energy consumption, reducing production costs and wide application prospects.

Inactive Publication Date: 2010-02-17
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, domestic research on the development mechanism of test tube bulbs and the mechanism of dormancy release is still unclear, and the research on the technology of test tube bulb stem extraction is still in a blank stage

Method used

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  • Culture method of oriental lily test tube bulbs
  • Culture method of oriental lily test tube bulbs
  • Culture method of oriental lily test tube bulbs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Configure the sucrose content as 60g·L -1 MS solid medium.

[0025] Select the disease-free and trauma-free healthy bulbs of the Oriental Lily Siberia (Siberia), peel off the outer scales, and leave the scales in the middle of the bulbs, sterilize in 0.1% mercuric chloride solution for 6 minutes, and rinse with sterile water 3 backups

[0026] (1) The scales of test tube bulbs were used as the initial explants for bulb regeneration to directly induce bulb formation. Induction medium containing 1 mg·L -1 6-BA, 0.1mg·L -1 The sucrose content of NAA is 60g·L -1 MS solid medium.

[0027] (2) After 2 and a half months, the regenerated bulbs were peeled off from the mother scales, and put into the subculture medium for cultivation. Each subculture time was 75 days, and it was cultivated twice. See the bulbs obtained. figure 1 As shown in the photo, the subculture medium contains 05mg·L -1 6-BA, 02mg·L -1 The sucrose content of NAA is 60g·L -1 MS solid medium.

[00...

Embodiment 2

[0034] Select the disease-free and trauma-free healthy bulbs of the Oriental lily "Sorbonne", peel off the outer scales, and leave the middle part of the bulbs, sterilize in 0.1% mercuric chloride solution for 6-10 minutes, and rinse with sterile water. Rinse 3-4 times for later use

[0035] (1) The sterilized scales were used as the initial explants, and inoculated in 1.5 mg·L -1 6-BA, 0.5mg·L -1 The sucrose content of NAA is 60g·L -1 MS solid medium.

[0036] (2) After 3 months, the regenerated bulbs were peeled off from the mother scales and put into the subculture medium for cultivation. The subculture medium contained 1 mg·L -1 6-BA, 1mg·L -1 The sucrose content of NAA is 60g·L -1 MS medium, each subculture time is 3 months.

[0037] (3) The test tube bulbs subcultured twice were placed in the culture medium (MS+0.5mg L-16-BA++60g L-1 sucrose) for inducing stem pumping, and treated at 15°C for 2 weeks.

[0038] (4) Low temperature treatment at 4°C for 9 weeks to r...

Embodiment 3

[0043]Select the disease-free and trauma-free healthy bulbs of Oriental Lily Siberia (Siberia), peel off the outer scales, and leave the scales in the middle of the bulbs, sterilize in 0.1% mercuric chloride solution for 10 minutes, and rinse with sterile water 4 times backup

[0044] (1) The scales of test tube bulbs were used as the initial explants for bulb regeneration to directly induce bulb formation. Induction medium containing 8 mg·L -1 6-BA, 7.5mg·L -1 The sucrose content of NAA is 60g·L -1 MS solid medium.

[0045] (2) After two and a half months, the regenerated bulbs were peeled off from the mother scales, and put into the subculture medium for cultivation, and the subculture time was 80 days each time. The subculture medium contains 7 mg·L -1 6-BA, 8.5mg·L -1 The sucrose content of NAA is 60g·L -1 MS solid medium.

[0046] (3) Place the test tube bulbs subcultured twice (circumference diameter about 5cm; weight 1.5-2.0g) in the culture medium (containing 8...

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Abstract

The invention relates to a culture method of oriental lily test tube bulbs. An MS culture medium is cultured under the darken condition with the temperature of 25 DEG C, wherein the MS culture mediumcontains 0.1-10.1 mg/L<-1> of 6-benzyladenine, 0.1-10.0 mg/L<-1> of rhodofix and 60 g/L<-1> of sucrose. Small lily bulbs are directly induced from explants, subculture is carried out twice, and a testtube bulb with the diameter of 4-5 cm and the weight of more than 1.5g is formed after 5 months. Temperature-variable treatment is carried out on the bulb through 6-BA combination for 8-15 days at the temperature of 10-15 DEG C, then low-temperature storage is carried out for 50-70 days at the temperature of 3-5 DEG C, and the dormancy is relieved. The stooling rate is more than 95% after the bulb is planted in fields. The diameter of the bulb diameter reaches 10-12 cm after one growing period. By adjusting and controlling the shape development of the test tube bulb and carrying out 6-BA combination temperature-variable induction, the dormancy is relieved at low temperature, the stooling rate of the oriental lily test tube bulb in the fields is enhanced, and the expansion speed of the bulb is increased by one time; the 3 growing periods of the culture time of detoxicating stock seeds in the part is shortened to 2 growing periods, therefore, the production cost is decreased by nearly 1/3.

Description

technical field [0001] The invention relates to agricultural biotechnology, in particular to a method for cultivating oriental lily test tube bulbs. Background technique [0002] Lily (Lilium spp.) is a perennial bulbous flower with underground bulbs belonging to the family Liliaceae (Lilium). It is one of the world-renowned ornamental flowers and occupies an important position in the international flower market. In recent years, my country's lily cut flower industry has developed rapidly, however, lily bulbs have always relied on imports. [0003] The production and planting of virus-free lily test tube bulbs is one of the core steps in the commercial production of lily bulbs. After a test tube bulb with a circumference of 3-5cm passes through a growth cycle (6-7 months), the circumference stem can grow to 6-9cm. The bulbs of this size go through another growth cycle, and the circumference will reach 10-12cm. The bulb circumference must go through the third growth cycle t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 刘春明军穆鼎
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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