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Tissue culture method for fastly reproducing saplings of Rubus spp (Tulaming or Xiumeite) and blackberry (Heibati)

A technology of tissue culture rapid propagation and production method, which is applied in the field of rapid seedling propagation, can solve the problems of breeding seedling speed and quantity limitation, difficulty, low survival rate, etc.

Inactive Publication Date: 2002-06-26
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
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Problems solved by technology

[0003] Raspberry Tulameen, Summit and Blackbuttee were successfully introduced and domesticated in my country from Oregon, USA. Seedling rapid propagation technology has become the main limiting factor restricting popularization and cultivation. Raspberry Tulameen, At present, Summit can only use the root tiller method to raise seedlings. Each raspberry Tulameen and Summit can breed more than 20 seedlings a year at most; although blackberry Black buttee can also carry out layering and cuttings to breed seedlings in addition to root tillers, the speed and quantity of seedlings are slow. Limited and unable to meet the high demand of the market
In addition, when asexual reproduction techniques such as cuttings and layering are used, the survival rate of Tulameen and Summit is low and difficult

Method used

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Embodiment Construction

[0019] Below in conjunction with embodiment the present invention is described in further detail.

[0020] According to the technical scheme of the present invention:

[0021] 4.1. Utilize the explants of three varieties of Raspberry Tulameen, Summit and Blackbuttee to carry out tissue culture and rapid breeding to produce seedlings, including utilizing Raspberry Tulameen (Tulameen) The explants of three varieties of , Summit and Blackbuttee were used for tissue culture to produce virus-free seedlings.

[0022] 4.2 Tissue culture rapid breeding production seedlings of three varieties of raspberry Tulameen, Summit and Blackbuttee and tissue culture virus-free seedling production adopt (1) selection of explants (2) preparation of medium; (3) explant pretreatment and disinfection; (4) inoculation; (5) cultivation (6) propagation; (7) induction of rooting; The culture conditions are that after inoculation, they are placed in a light culture room for cultivation, with 10h to 12h ...

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Abstract

A tissue culture method for quickly reproducing the raspberries of Tulameen, summit and Blackbuttee includes such steps as choosing explant; preparing the culture medium for induced differentiation from 6-benzyladenine, naphthylacetic acid, cane sugar, agar and activated carbon besides MS culture medium and the culture medium for induced rooting from naphthylacetic acid, can sugar and agar beside 1 / 2 MS culture medium; pretreating the explant, disinfecting; inoculating; culturing in optical culture room at 25 + / - 2 deg.C under sunlight for 10-12 hr every day; induced differentiation; induced rooting; and culturing sapling.

Description

[0001] 1. Field [0002] The present invention belongs to a production technique for rapid propagation of seedlings of three varieties of raspberry Tulameen, Summit and Blackbuttee, specifically utilizing the production technology of raspberry Tulameen (Tulameen) ), Summit and Blackbuttee (Blackbuttee) three varieties of explants were used for tissue culture, and in the invented medium formula matrix, callus tissue, clustered shoots and rooting were induced to achieve proliferation The purpose of rapid propagation and detoxification. 2. Background technology [0003] Raspberry Tulameen, Summit and Blackbuttee were successfully introduced and domesticated in my country from Oregon, USA. Seedling rapid propagation technology has become the main limiting factor restricting popularization and cultivation. Raspberry Tulameen, At present, Summit can only use the root tiller method to raise seedlings. Each raspberry Tulameen and Summit can breed more than 20 seedlings a year at most;...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈铁山郭军战朱首军武苏里彭少兵王丽玲
Owner NORTHWEST A & F UNIV
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