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A method for tissue culture and rapid propagation of willow stem segments

A technology of tissue culture rapid propagation and willow drilling, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low operability and repeatability, general growth status, etc., and improve the efficiency of breeding seedlings Effect

Active Publication Date: 2016-05-18
INST OF FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the problems of normal growth, poor operability and low repeatability in the existing tissue culture methods of willow willow, the present invention aims to provide a method for tissue culture and rapid propagation of willow willow stem segments

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Screening the medium for inducing clumping buds

[0032] Four mediums, WPM, DKW, 2 / 3DKW and 1 / 2NRM were used as the basic medium to select the basic medium suitable for the induction culture of the stem segments of Dianthus spp. Prepare a medium with the same types of hormones and the same concentration with the above four basic mediums. The hormone used is 6-BA (6-benzylaminmopurine) with a final concentration of 1.0 mg / L plus NAA with a final concentration of 0.1 mg / L (Naphthaleneacetic acid, 1-Naphthaleneacetic acid), that is, prepare the following medium (pH value is 5.80):

[0033] 1. WPM+6-BA1.0mg / L+NAA0.1mg / L medium

[0034] 2. DKW+6-BA1.0mg / L+NAA0.1mg / L medium

[0035] 3. 2 / 3DKW+6-BA1.0mg / L+NAA0.1mg / L medium

[0036] 4. 1 / 2NRM+6-BA1.0mg / L+NAA0.1mg / L medium

[0037] Cultivation of cluster buds was carried out on the stem segment (3cm in length, with one axillary bud) of Dianthus sibiricum that year. The culture conditions were 10h-12h of light per day and temper...

Embodiment 2

[0041] Example 2 Screening of Dedifferentiation Medium

[0042] Four mediums, WPM, DKW, 2 / 3DKW, and 1 / 2NRM were used as the basic medium to select the basic medium suitable for the dedifferentiation culture of the stems of the willow. Prepare the medium with the same types and concentrations of the above four basic mediums. The additives used are the final concentration of 2.0mg / L 6-BA, 0.1mg / L NAA, 4.5g / L agar and 20g / L glucose , That is, prepare the following medium (pH value is 5.80):

[0043] 1. WPM+6-BA2.0mg / L+NAA0.1mg / L+agar 4.5g / L+glucose 20g / L medium

[0044] 2. DKW+6-BA2.0mg / L+NAA0.1mg / L+agar 4.5g / L+glucose 20g / L medium

[0045] 3. 2 / 3DKW+6-BA2.0mg / L+NAA0.1mg / L+agar 4.5g / L+glucose 20g / L medium

[0046] 4. 1 / 2NRM+6-BA2.0mg / L+NAA0.1mg / L+agar 4.5g / L+glucose 20g / L medium

[0047] The clumping buds obtained in Example 1 were inoculated on the above 4 kinds of culture mediums for dedifferentiation culture, and the culture conditions were 10h-12h light per day, and the temperature wa...

Embodiment 3

[0051] Example 3 Using the method of the present invention to carry out tissue culture and rapid propagation of the stems of Willow

[0052] On April 20, 2014, in Huanren County, Benxi City, the original branches of Dianthus sibiricum were collected. The diameter of the branches should be more than 3mm. The collected branches should be cut into 3cm stems without pests and diseases. There is an axillary bud, and the tissue culture and rapid propagation of the stems of the willow will begin:

[0053] (The pH values ​​of the clumping bud induction medium, dedifferentiation medium, differentiation medium and rooting medium used below are all 5.80.)

[0054] 1) Disinfection of explants: Disinfect the collected stems for 7 minutes with a mercury solution with a mass percentage of 0.3%.

[0055] 2) Cluster bud induction culture: 100 sterilized stem segments are inoculated on the cluster bud induction medium, which is based on DKW medium and contains 1.0 mg / L (final concentration, The same b...

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PUM

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Abstract

The invention belongs to the field of plant tissue culture, and discloses a chosenia stem section tissue culture and rapid propagation method which comprises the following steps: collecting chosenia one-year stem sections for successive 1) sterilization of explants, 2) induction culture of cluster buds, 3) proliferation culture of the cluster buds, 4), rooting culture of the cluster buds and 5) acclimatization and transplant to obtain chosenia tissue culture seedling. The method breaks through the bottleneck that chosenia only can breed by seeds and the survival rate is low, can help to break the chosenia endangered statu, and forms chosenia mass rapid reproduction situation. At the same time, by the use of tissue culture and rapid propagation technology, culture conditions are controllable, anniversary test or production are feasible, growth is fast, cycle is short, repeatability is strong, under breeding conditions in factories, millions of strains of finished and virus-free seedlings can be bred by each chosenia strain in one year, and chosenia seedling breeding efficiency can be greatly improved.

Description

Technical field [0001] The invention belongs to the field of plant tissue culture, and specifically relates to a method for tissue culture and rapid propagation of the stem segments of Salix sibiricum. Background technique [0002] Chosenia arbutifolia (Chosenia arbutifolia), also known as Korean willow and red twig, is a large deciduous tree of the genus Chosenia in the Willow family. It is a single species. This species is now endangered and belongs to an endangered tree species of Vulnerable (V) level, and it is a wild plant under national level II key protection. Dianthus genus is mainly distributed in northeastern Asia, starting from the Arctic Circle in the north, passing through Eastern Siberia in the south, and entering our country. East and West are distributed in eastern Russia and its islands, northeastern China, northern Japan to central and northern Korea. Due to the deterioration of the ecological environment, some areas have been extinct, and some areas have only...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 李振坚孙振元季萍倩韩蕾刘慧兰王正超刘俊祥崔敏
Owner INST OF FORESTRY CHINESE ACAD OF FORESTRY
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