Method for tissue culture and rapid propagation of stems of cinnamomum kanehirae hay

A technology of tissue culture rapid propagation and camphor, which is applied in the field of plant tissue culture, can solve the problems of immature technology, inability to carry out large-scale breeding, low survival rate, etc., and achieve the goal of reducing dryness and death, normal shape, and reducing pollution rate Effect

Inactive Publication Date: 2015-08-26
广州甘蔗糖业研究所湛江甘蔗研究中心
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Problems solved by technology

[0006] Aiming at the problems that Cinnamomum camphora species are endangered, the existing cutting and grafting seedling raising techniques are immature, the survival rate is low, and large-scale breeding cannot be carried out, the present invention provides a method for tissue culture and rapid propagation of Cinnamomum camphora stem segments

Method used

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  • Method for tissue culture and rapid propagation of stems of cinnamomum kanehirae hay
  • Method for tissue culture and rapid propagation of stems of cinnamomum kanehirae hay
  • Method for tissue culture and rapid propagation of stems of cinnamomum kanehirae hay

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Influence of different disinfectant treatment and disinfection time on the disinfection effect of Cinnamomum camphora stem

[0043] Collect the young side branches with full axillary buds of the healthy mother tree that have not yet germinated, remove the leaves and keep 1~2 cm petioles, cut into 4~6 cm long stems, first soak in washing powder water for 30 min, and then use 1000 Soak in mg / L thiophanate-methyl for 10 min (blank: when not soaking in 1000 mg / L thiophanate-methyl, rinse with running water for 30 min instead). Soak in 75% alcohol for 30 s on the ultra-clean workbench, rinse with sterile water 3 times, then treat with 0.1% mercury liters for 5-15 minutes, and rinse with sterile water 4-6 times. The ends and petioles were cut off 1 cm each, and cut into stem segments with 1-2 axillary buds, and inoculated on the spare medium.

[0044] Each treatment was inoculated with 30 shoots and stems, and the experiment was repeated 3 times. Thirty days after the...

Embodiment 2

[0051] Example 2 Effect of Different Induction Medium on Induction of Cinnamomum Cinnamomum Stem

[0052] According to the disinfection method in Example 1, collect the young side branches with full axillary buds of the healthy mother tree that have not yet germinated, remove the leaves and retain 1~2 cm petioles, cut into 4~6 cm long stems, first use washing powder Soak in water for 30 min, and then soak with 1000 mg / L thiophanate methyl for 10 min. Soak in 75% alcohol for 30 s on the ultra-clean workbench, rinse 3 times with sterile water, then rinse with 0.1% mercury for 10 min, rinse 4-6 times with sterile water. The ends and petioles were cut off 1 cm each, and cut into stem segments with 1-2 axillary buds, and inoculated in the following induction medium (see Table 2 for the specific formulation of the induction medium) for bud induction culture. The cultivation light intensity is 1500-2000 lux, and the light time is 12 hours / day.

[0053] The induction medium is compose...

Embodiment 3

[0060] Example 3 Effect of Different Proliferation Medium on Proliferation and Culture of Cinnamomum camphora

[0061] The shoots induced in Example 2 were inoculated into a proliferation medium (see Table 3 for the specific formula of the proliferation medium) for proliferation culture. The cultivation light intensity is 1500-2000 lux, and the light time is 12 hours / day.

[0062] The proliferation medium is composed of a combination of a basic medium and hormones, and the basic medium includes the following components: MS medium, Vc 2.0 mg / L, biotin 0.30 mg / L, and L-cysteine ​​5.0 mg / L L, sucrose 30g / L, agar 7 g / L, pH 5.8.

[0063] For each treatment, 40 shoots were inoculated, and the experiment was repeated 3 times. For each generation of bud proliferation and cultivation (ie every 30 days), the corresponding proliferation coefficient and bud growth are calculated.

[0064] Table 3 Effects of different hormone combinations on the proliferation of Cinnamomum camphora buds

[006...

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Abstract

The invention discloses a method for tissue culture and rapid propagation of stems of cinnamomum kanehirae hay. The method comprises the steps of S1. disinfecting explants of the stems of cinnamomum kanehirae hay, inoculating the disinfected explants into an induction medium, and carrying out induction culture; S2. carrying out proliferating cultivation, namely, sequentially inoculating the explants subjected to induction culture into a proliferation medium A and a proliferation medium B, and carrying out proliferating cultivation; S3. carrying out strong seedling culture; and S4. carrying out rooting culture. According to the method, the conditions that fungus and bacterial pollution is easily caused to the explants of cinnamomum kanehirae hay in the primary culture process can be avoided, and the germination rate can be ensured while the pollution rate is reduced. Moreover, the concentration of the hormone of the proliferation medium A is slightly high, so that calluses can be induced; the calluses are inoculated to the proliferation medium B with relatively low hormone concentration, withering and death of leaves can be reduced, a large quantity of calluses can be differentiated, the month propagation coefficient achieves 4-6 times, the form is normal in the propagation process, large-scale industrial production can be met, and the nursery stock breeding efficiency can be greatly improved.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, in particular to a method for tissue culture and rapid propagation of cinnamon camphor stem segments. Background technique [0002] Cinnamomum camphor ( Cinnamomum Kanehirae Hay ) belonging to Lauraceae ( Lauraceae ) Cinnamomum, also known as black camphor, is a native evergreen broad-leaved tree species native to Taiwan, China. Cinnamomum camphora has a straight trunk, tall tree body and changeable primary leaves. It has good ornamental value and is a landscape tree with great development potential. Cinnamomum camphor wood also has a special fragrance, and is not easy to decay, fine material, interlaced texture and easy to plan. It is a famous building, furniture and carving craft material. Cinnamomum camphora oil can be extracted from the whole plant. [0003] The parasitic fungus on the camphor tree - Antrodia cinnamomea, known as "the ruby ​​in the deep forest", contains mor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈月桂官锦燕谭嘉娜罗清文杨俊贤罗剑飘黄海英
Owner 广州甘蔗糖业研究所湛江甘蔗研究中心
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