371 results about "Bacterial pollution" patented technology

A fermentation process for the production of ethanol from natural sources, such as corn, comprising introducing a fermentable sugar and an inoculant, and a bacteriophage cocktail into a fermentation system and introducing a cocktail comprising one or more lytic bacteriophage is added to one or more of the fermentable sugar, the inoculant, or the fermentation system. Bacteriophages that infect and lyse the bacteria that contaminate fermentable sugars are selected. The bacteriophage cocktail is added in an amount effective to substantially prevent growth of these bacteria.

The invention provides a method for preparing mesenchymal stem cells of menstrual blood. The method comprises steps as follows: female menstrual blood is collected and taken as a raw material and is subjected to sterile processing; stem cells in the menstrual blood are separated; the mesenchymal stem cells of the menstrual blood are cultured; the mesenchymal stem cells of the menstrual blood are cryopreserved; and the mesenchymal stem cells of the menstrual blood are recovered. The method has the technical characteristics as follows: the female menstrual blood is collected and stored in a preservative fluid; by means of a bacterial pollution prevention method, the pollution probability is reduced from a collection source, a collecting cup is repeatedly washed by sterile water, and the pollution probability is effectively reduced; with the adoption of a differential centrifugation method, bacteria in the menstrual blood are removed as far as possible; a sample is repeatedly separated by HES (hydroxyethyl starch), and the maximum quantity of mesenchymal stem cells of the menstrual blood can be obtained; a serum-free medium is utilized for culturing, components of animal origin are reduced, the cell performance is stable, the in-vitro long-term culturing process of the mesenchymal stem cells of the menstrual blood can be kept, and cellular morphology, multiplication capacity, MSC (mesenchymal stem cell) surface marker expression, differentiation capacity and the like are maintained. The method is simple, practical and convenient to operate, the maximum quantity of required stem cells can be obtained, and the stem cells are successfully cultured.

The invention relates to a colorimetric method for detecting bacterial or fungal pathogens by detecting peptidoglycan or (1-3)-β-D-glucan in a sample.

The invention discloses an edible film-coating freshness preservation agent used for megalobrama amblycephala, which belongs to the technical fields of the freshness preservation and the processing of aquatic products. The freshness preservation agent is a water solution prepared by dissolving the following components in water, wherein each component and the concentration thereof in the water solution are as follows: 14-16g/L of sodium alginate, 95-105g/L of glycerol and 40-60g/L of ascorbate. The invention also discloses a use method of the freshness preservation agent. After the film-coating freshness preservation agent is used for the film coating of the megalobrama amblycephala, a layer of film can be formed on the surface of the megalobrama amblycephala and acts as the functions of reducing bacterial pollution, prohibiting microbial growth, attenuating air oxidation and slowing down the lipid oxidation rate, and the effect of freshness preservation is obviously superior to that of an ordinary low-temperature preservation method. The use method of the film-coating freshness preservation agent is simple, the equipment investment is little, the operation site is small, and the film-coating freshness preservation agent can be suitable for industrial mass production.

The invention provides a method for preparing a decidua mesenchymal stem cell. The method comprises the following steps: performing placenta asepsis by taking the placenta of a male full-term fetus as a raw material; separating a decidual tissue; identifying the decidual tissue and identifying whether the decidua mesenchymal stem cell comes from a maternal tissue; culturing the decidua mesenchymal stem cell; freeze-storing the decidua mesenchymal stem cell; and reviving the decidua mesenchymal stem cell. The method provided by the invention has the technical characteristics that the sampled fetus is male fetus placenta; after the decidual tissue is obtained, sex determination is performed to identify whether the decidual tissue comes from a parent body and is free of pollution of a daughter tissue; by adopting a bacterial pollution prevention method, the pollution possibility is reduced from a sampling source; the surface is washed for a plurality of times, so that the pollution possibility is effectively reduced; a single enzyme is used for digestion to simplify the procedures; a serum-free medium is used for culture to reduce the use of an animal source component; the cell is stable in performance; a long-term in-vitro culture process of the decidua mesenchymal stem cell can be maintained; and the cellular morphology, multiplication capacity, MSC surface marking expression capacity, differentiative capacity and the like of the cell can be maintained.

The invention discloses a preservative for aquatic products, and is particularly suitable for fish products. The preservative is prepared by dissolving the following components in water: 50 to 75 weight percent of sodium hexametaphosphate, sodium tripolyphosphate and sodium pyrophosphate, 15 to 20 weight percent of sodium alginate, 5 to 10 weight percent of ascorbic acid and 2 to 10 weight percent of glycerol, wherein the weight ratio of the sodium hexametaphosphate to the sodium tripolyphosphate to the sodium pyrophosphate is 10:2:1. The coating preservative is used for forming a layer of film on the surfaces of the aquatic products after the aquatic products are coated to achieve the effects of reducing bacterial pollution, inhibiting the growth of microbes and weakening air oxidation, and a refreshment effect is obviously superior to that of the ordinary low-temperature preservation method. A using method for the preservative is simple, the preservative can be suitable for industrial batch production, the investment of equipment is low, and an operation site is small.

The invention aims to provide nano modified polylactic acid with high toughness and good flame resistance and a preparation method thereof. The nano modified polylactic acid is prepared by mixing the following raw materials in percentage by weight: 45 to 94.6 percent of polylactic acid, 5 to 45 percent of nano particles, 0.2 to 5 percent of thermal stability and 0.2 to 5 percent of processing aid. The preparation method comprises the following steps of: A, putting the weighed polylactic acid, nano particles, thermal stability and processing aid into a high-speed mixer in a ratio and mixing the raw materials; and B, conveying the raw materials mixed in the step A to a double-screw extruder, and melting, extruding and granulating the raw materials under high-speed shearing, mixing and conveying of screws to obtain a composite material. The polylactic acid is modified by using the nano particles, so the impact resistance, flame resistance, ageing resistance and bacterial pollution resistance of the polylactic acid composite material can be improved, and the application range of the polylactic acid composite material is enlarged.

The invention relates to a current enzyme electrode for detecting catalase-positive bacteria and a preparation method thereof in the technical field of biological sensing; the electrode comprises an electrode substrate, wiring terminals, electrode connection cables, a working electrode, a counter electrode, an insulation layer and reaction substrate films, and is characterized in that the wiring terminals, the electrode connection cables, the working electrode and the counter electrode are respectively arranged between the electrode substrate and the insulation layer; the working electrode is arranged in the middle of the electrode with a gap left between; one end of each electrode connection cable is respectively connected with the working electrode and the counter electrode, and the other end thereof is respectively connected with the two wiring terminals; and the reaction substrate films are arranged above the working electrode and the counter electrode. The electrode which is prepared by the method has the advantages of high sensitivity and no need of bacterial culturing, can realize continuous detection of a plurality of samples by replacing different disposable electrode strips, is simple to operate, is easy for industrial popularization, and has important practical value in food bacterial pollution monitoring.

A process for preparing the injection of carnosine features that the chitosan and oligose are used for adsorbing the modified protein and fat to improve the purity of product, and the 6-aminopurine phosphate is used as the protector for increasing the sterilizing temp and time to 121 deg.C and 30 min, so having no bacterial pollution.

The invention relates to a medical cotton swab asepsis storage box, and discloses a storage and taking device through which cotton swabs can be conveniently taken out and bacterial pollution can be effectively prevented. The medical cotton swab asepsis storage box is characterized in that a fixing frame is arranged on upper edges on the left side and the right side of a main box through fixing grooves, a limiting plate is arranged on the fixing frame and parallel to the bottom of the main box, a cover plate is arranged on the main box and located below the limiting plate, the cover plate is provided with a taking outlet, a taking-out cover is hinged to the portion, on one side of the taking outlet, of the cover plate through a connection strip and located above the taking outlet, multiple partition plates are distributed on the cover plate at equal intervals and located in the main body, the distance between every two adjacent partition plates is slightly larger than the diameter of each cotton swab, multiple baffles are evenly distributed on the taking-out cover and arranged between the partition plates in a one-to-one corresponding mode, the taking outlet is divided by the baffles into multiple small windows, multiple shifting needles are evenly arranged on the cover plate and located between the partition plates correspondingly, and the shifting needles are located on one side of the taking outlet.

A beverage bottle boxing machine comprises a rack, a rotating disc driven by a driving device to rotate is installed in the center of the rack, multiple processing stations for containing packaging boxes are concavely formed in the rotating disc, the periphery of the rotating disc is sequentially connected with a packaging box loading device, a beverage bottle feeding and upper cap loading device, a lower cap loading device, a lower pressing device, a bottle cap loading device, a bottle cap screwing device and a discharging output device, and the processing stations sequentially pass through the devices along with rotation of the rotating disc. The beverage bottle boxing machine is high in automation degree, production efficiency is improved, labor is saved, cost is reduced, bacterial pollution caused by manual contact in the boxing process is reduced, and the beverage bottle boxing machine is suitable for wide application and popularization.

The invention discloses a method for tissue culture and rapid propagation of stems of cinnamomum kanehirae hay. The method comprises the steps of S1. disinfecting explants of the stems of cinnamomum kanehirae hay, inoculating the disinfected explants into an induction medium, and carrying out induction culture; S2. carrying out proliferating cultivation, namely, sequentially inoculating the explants subjected to induction culture into a proliferation medium A and a proliferation medium B, and carrying out proliferating cultivation; S3. carrying out strong seedling culture; and S4. carrying out rooting culture. According to the method, the conditions that fungus and bacterial pollution is easily caused to the explants of cinnamomum kanehirae hay in the primary culture process can be avoided, and the germination rate can be ensured while the pollution rate is reduced. Moreover, the concentration of the hormone of the proliferation medium A is slightly high, so that calluses can be induced; the calluses are inoculated to the proliferation medium B with relatively low hormone concentration, withering and death of leaves can be reduced, a large quantity of calluses can be differentiated, the month propagation coefficient achieves 4-6 times, the form is normal in the propagation process, large-scale industrial production can be met, and the nursery stock breeding efficiency can be greatly improved.

Bacterial contamination of industrial water systems lead to biofouling by biofilms and corrosion from bacterial induced corrosion. This invention provides a method for control of fouling and contamination of industrial water systems caused by bacteria. Prevention or reduction of process interruptions and general contamination, fouling and corrosion is achieved by the destruction of targeted problematic bacteria with naturally occurring, non-engineered bacteriophage virulent for targeted bacteria. The invention also provides for in-situ confirmation of the proper identification of target bacteria and a mobile laboratory adapted to implement the method.

The invention provides a method for producing an avian influenza vaccine by using cells cultured by a spherical microcarrier bioreactor. The method comprises the following steps of: selecting seed cells; passaging cells reproduced in a spinner bottle; harvesting the cells in the spinner bottle; inoculating the cells; culturing the inoculated cells in the bioreactor; inoculating avian influenza virus; reproducing the avian influenza virus; collecting avian influenza virus liquid; inactivating the avian influenza virus liquid; preparing an avian influenza cell vaccine; and immunizing the avian influenza cell vaccine. By the method, the defects that the avian influenza vaccine produced by the traditional process is long in production period, large in inter-batch difference, high in endotoxin content and influenced by the supply of embryo eggs, and a tablet carrier is difficult to amplify are overcome; and the prepared avian influenza vaccine product is small in inter-batch difference, free from bacterial pollution, low in endotoxin content and formaldehyde content, stable in quality and short in the production period, and the tablet carrier is easy to amplify.

The invention relates to medicine smearing equipment, in particular to uniform medicine smearing equipment for affected parts in the dermatology department. The technical problem to be solved is to provide the uniform medicine applying equipment for the affected part in the dermatology department, which is convenient to operate, saves manpower, reduces bacterial pollution, improves the applying effect and reduces waste by quantitative extrusion. According to the technical scheme, the equipment for evenly smearing the medicine on the affected part for the dermatology department comprises a supporting frame and a smearing assembly, wherein the smearing assembly is arranged on one side of the bottom of the supporting frame; and a material extruding assembly, wherein the top of the supporting frame is provided with the material extruding assembly. According to the device, firstly, people install ointment needing to be wiped on the material extruding assembly on the supporting frame, the material extruding assembly is moved forwards to extrude the ointment, the ointment falls on the smearing assembly, then people hold the device by hand to paste the smearing assembly on an affected part to roll, the ointment is smeared on the affected part for treatment, and the effect of keeping clean and sanitary is achieved.

The invention relates to an oral cleaning device provided with a protective cover. The protective cover can achieve an effect of preventing dust or bacterial pollution, and the surface of the protective cover is a mirror surface capable of reflecting light and forming an image, so that the use convenience is achieved; the oral cleaning device is convenient to carry, so that teeth and oral cavity can be cleaned anytime and anywhere.

The invention discloses a snap bean preservative and belongs to the technical field of preservatives. The snap bean preservative is characterized by consisting of nobiletin, chitosan, sodium alginate, ascorbic acid, phytic acid, calcium chloride and water. The snap bean preservative is prepared from various selected ingredients, the ingredients give full play to a synergistic effect on the premise of exerting respective functions, and the formula is reasonable; during a use process, the snap bean preservative can be used after the above ingredients are mixed uniformly, is a spray-type preservative and is simple to operate; meanwhile, compared with the prior art, the snap bean preservative is good in film-forming properties, is capable of effectively reducing water evaporation, reducing bacterial pollution, reducing rusty spots, reducing the malondialdehyde content, keeping the rigidity and chlorophyll content of snap beans and prolonging the preservation time, and can be stored at the temperature of 9+/-1 DEG C for 30 days.