This invention discloses an automated
stem cell culture chamber with precise temperature and
humidity control, comprising a chamber body, a culture tray, a
diffusion frame positioned above the culture tray, an adjusting and sealing mechanism, and an
oxygen absorption mechanism. The
diffusion frame has a first and a second permeable membrane along the arrangement direction of the culture containers. The first permeable membrane forms an
oxygen supply interface, and the second permeable membrane forms a hypoxia
communication interface with the
oxygen absorption box. The adjusting and sealing mechanism limits the effective
exposure area of the two permeable membranes respectively through a first sealing plate. The
oxygen absorption box contains oxygen-absorbing material and can be moved closer to or further away from the second permeable membrane to change the volume of the hypoxia buffer chamber. A second sealing plate is provided on the ventilation side of the
oxygen absorption box to adjust the ventilation opening area. Thus, without changing the position of the culture tray, by adjusting the
gas exchange conditions on the
oxygen supply side and the hypoxia side, an adjustable oxygen
concentration gradient environment is formed above the culture tray, suitable for
stem cell hypoxia induction, differentiation comparison, and
drug response culture experiments.