The invention provides an experimental apparatus for simulation research on water quality and quantity of biotical retained elements under an artificial rainfall condition. The experimental apparatus comprises a rainwater storage tank, a water pump, an aqueduct, a supporting frame, a water quantity regulation tank, a valve, a water outlet pipe, a spray head, rainfall holes, a porous water distribution plate, an experimental soil bin, a small sampling perforated pipe and a large sampling perforated pipe, wherein the lower outlet of the rainwater storage tank is connected with a water inlet of the water pump; a water outlet of the water pump is communicated with the water quantity regulation tank arranged on the supporting frame through the aqueduct; the outlet of the water quantity regulation tank is provided with the valve and connected with the spray head through the water outlet pipe; the porous water distribution plate which is provided with a plurality of rainfall holes is arranged under the spray head and above the experimental soil bin; and the small sampling perforated pipe is positioned on the central part of the experimental soil bin, while the large sampling perforated pipe is positioned on the lower part of the experimental soil bin. The experimental apparatus for the water quality and quantity simulation research on the biotical retained elements under the artificial rainfall condition has a simple structure, can regulate rainfall intensity and uniformity, and realize water sampling of soil in different time and different structural space, and has an application prospect in the field of environment engineering and ecological engineering.