The invention relates to a stratified flow simulation test water channel system which includes a reservoir, a submersible pump, a flowmeter, a valve switch, a water channel, a hydraulic jack, a position regulator in the water channel, a position-adjustable water inlet, a grid net, a pulling gate, a digital temperature chain and a sampling tube, a backwater sand punching pipe, a multi-channel recorder outside the water channel, a pump-absorption water sample acquisition case, a drain aperture elevation adjusting device and a position-adjustable water outlet. The water channel system has a simple structure and is easy to operate. The system can simulate a moving process of a stratified flow at the beginning of an inflow stage, and can also simulate the moving process of a stratified flow under the release of a valve flow, a stratified flow having the water inlets at different depths, a stratified flow having different bottom slop coarse degrees, and a stratified flow caused by different shapes and different factors. The system, through addressing the problem of singularity in stratified flow simulation types in traditional stratified flow tests, successfully achieves tests of different stratified flow simulation types, and increases reliability and accuracy of density flow analysis and research.