The utility model discloses a separating type operation panel and an animal experiment device thereof, and belongs to the technical field of animal experiments, the separating type operation panel comprises a base plate, and the base plate is provided with a positioning hole; the separating plate is arranged in the positioning hole; the two constraining assemblies are symmetrically arranged on the left side and the right side of the separating plate and used for constraining the four limbs of the experimental subject; the head positioning
assembly is used for restraining the head of the
experiment object, and the head positioning
assembly comprises two limiting units and a transverse sliding rail arranged at one end of the separating plate. According to the utility model, the separating plate and the substrate are designed, and the separating plate and the substrate are connected in a clamping manner, so that the stable connection between the separating plate and the substrate is ensured, and rapid disassembly and
assembly are facilitated, and therefore, experimental objects can be conveniently transferred on different stations; the binding assembly and the head positioning assembly both adopt sliding adjustment design, the positions can be flexibly adjusted according to the
body type of an
experiment object and experiment requirements, and the flexibility and adaptability of experiment operation are improved.