The invention discloses a radiobiological functional experiment box which comprises a box body and matched fixing supports. The box body comprises a box rear plate structure, a box bottom bin structure, a three-dimensional fixing substrate structure, a sterile sleeve opening structure, a box chamber, box side plates and an incident center point. The matched fixing supports comprise an in-vitro cell experiment fixing support, an insertion piece fixing support, a clamshell type fixing support and a limb end locating type fixing support. Functions of the radiobiological functional experiment box include irradiation, anatomy, injection, temporary rearing and the like, the coverage of use objects is wide, sterile operation and ingenious fixing of irradiated portions can be achieved, and it is guaranteed that experimental subjects keep awake and alive. Multiple animals can be fixed and irradiated at the same time, so that specific operation time of experiments is shortened, and a dose is reduced. The radiobiological functional experiment box is reasonable in design, convenient and safe to operate, environmentally friendly and high in practicality and universality.